Protein Alignment PDZ-GEF and RAPGEF3

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: XP_011536054.1
• Gene: RAPGEF3 / 10411
• HGNCID: 16629
• Length: 938
• Species: Homo sapiens
• Alignment Length: 1116
• Identity: 264/1116 - (23%)
• Similarity: 405/1116 - (36%)
• Gaps: 427/1116 - (38%)

  Fly    84 EEDLAESMDSLTVR--DAVRD-CLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMV 145
            ||:|||::..|:.|  ||:.. .|.|.|.:|::|:::::.|....:||..:::.:|:|.|.:|::
Human   213 EEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLL 277

  Fly   146 FAVVDKAGTVVMSDGEELDSWSVLINGAVE-IEHANGSREELQMGDSFGILPTMDKLYHRGVMRT 209
            |....|||||:.|.|::..||.::..|:|. :.|..|....|..||.||.|..::.......:..
Human   278 FEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIIL 342

  Fly   210 KCDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASAS 274
            :.|:|.|:.:.:.|:.||....|..|.|.| |:|.||:|.| |:..|||.....:.|
Human   343 REDNCHFLRVDKQDFNRIIKDVEAKTMRLE-EHGKVVLVLE-RASQGAGPSRPPTPG-------- 397

  Fly   275 LNMKRGHVVIRGTPERLLQQLVE----ENSMTDP--TYVEDFLLTHRIFIQNPQEVTSKLLHWFD 333
               :..:.|:.||||::|:.|:|    ::|..||  |::.|||||||:|:.:.| :.:.|||.|.
Human   398 ---RNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQ-LCAALLHHFH 458

  Fly   334 LEQVDAHKTQ------ELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQL-- 390
            :|.....:.:      ..|.::.|:|..||..:.:....|.....||:....|:.|...||.|
Human   459 VEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLR 523

  Fly   391 ---------RLLHIAC---AAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYI 443
                     ..|...|   :.:.:.|:..:...::||||
Human   524 EQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPL-------------------------- 562

  Fly   444 SHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALE 508
                |||..   .::.||::
Human   563 ----PGSSC---AIQVGDKV--------------------------------------------- 575

  Fly   509 HGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDM 573
                                   |..||..|.:.|       |.:| .|:||.|..|..|:
Human   576 -----------------------PYDICRPDHSVL-------TLQL-PVTASVREVMAALA---- 605

  Fly   574 LLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQD--TKGGFMTLAPKR 636
                                                            |:|  |||
Human   606 ------------------------------------------------QEDGWTKG--------- 613

  Fly   637 RLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQ 701
               :.|.|:|                                             |.|
Human   614 ---QVLVKVN---------------------------------------------SAG------- 623

  Fly   702 SNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKA 766
                          ||.|     ||          |.|....|:......|....|..|
Human   624 --------------DAIG-----LQ----------PDARGVATSLGLNERLFVVNPQEV------ 653

  Fly   767 DQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERIS 831
                           ||::           |.                     ||||
Human   654 ---------------HELI-----------PH---------------------PDQL-------- 663

  Fly   832 FAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQD---FANFRQIESTEYV 893
                         .|.|.....|:||          :|.:||.|||..|   |.:..|:|...||
Human   664 -------------GPTVGSAEGLDLV----------SAKDLAGQLTDHDWSLFNSIHQVELIHYV 705

  Fly   894 DELFELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSM 958
            .....||. .....|.:|....|...:||.:|:|.......|.:::::|||:|.|.||.:|.||.
Human   706 LGPQHLRD-VTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSF 769

  Fly   959 FAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYP 1023
            ||::.||.:.|:|||..|||:||.|.::|::.|:.|:|||.|...|| |..|:|  ..|:|||.|
Human   770 FAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYR-LALAKL--SPPVIPFMP 831

  Fly  1024 IVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSS--PYDLLSILELKGQSPSNALFS 1086
            ::.||:||||.||.|.|:.||||||:||:|:..|:| |.|.|  |..|         ||..
Human   832 LLLKDMTFIHEGNHTLVENLINFEKMRMMARAARML-HHCRSHNPVPL---------SPLR---- 882

  Fly  1087 LNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDED 1151
             :::|....::....:       :|...:..||.:|            ....||:..||::.::.
Human   883 -SRVSHLHEDSQVARI-------STCSEQSLSTRSP------------ASTWAYVQQLKVIDNQR 927

  Fly  1152 LLHKFSLECEP 1162
            .|.:.|.|.||
Human   928 ELSRLSRELEP 938

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	33/109 (30%)
		RasGEFN	279..400	CDD:214571	41/146 (28%)
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237	10/91 (11%)
		RA_PDZ-GEF1	759..841	CDD:340483	8/81 (10%)
		RasGEF	871..1050	CDD:459872	78/181 (43%)
RAPGEF3	XP_011536054.1	DEP_Epac	100..238	CDD:239884	10/24 (42%)
		CAP_ED	262..371	CDD:237999	32/108 (30%)
		REM	407..526	CDD:100121	37/119 (31%)
		RasGEF	673..905	CDD:238087	96/267 (36%)