Protein Alignment PDZ-GEF and sos2

DIOPT Version :10

• Sequence 1: NP_609012.2
• Gene: PDZ-GEF / 33881
• FlyBaseID: FBgn0265778
• Length: 1573
• Species: Drosophila melanogaster
• Sequence 2: XP_002938172.3
• Gene: sos2 / 100497015
• XenbaseID: XB-GENE-1006170
• Length: 1333
• Species: Xenopus tropicalis
• Alignment Length: 1695
• Identity: 293/1695 - (17%)
• Similarity: 509/1695 - (30%)
• Gaps: 654/1695 - (38%)

  Fly    56 TMASNYASSLEAEEIDLSGL----------VESVVDSDEEDLAESMDSLTVRDAVRDCLEK---- 106
            |:::...|....||:.|..|          |:.|.:..::.....:|...:.|| :..:||
 Frog    37 TLSAKEDSLYYVEELILQLLNKLCIAQPRTVQDVEERVQKTFPHPIDKWAIADA-QSAIEKRKRR 100

  Fly   107 DPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWSVLIN 171
            :|.....:.:..||:...|.|...:::|.: .|:...:...::..||..|.:
 Frog   101 NPLLLPVDKIHPLLKEVLGYKIDYHVSLYI-VAVLEYISADILKLAGNYVFN------------- 151

  Fly   172 GAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTD--------YYRIQ 228
                |.|...::::::      :....||:...  |..:.||...|.::..:        ||.:.
 Frog   152 ----IRHFEITQQDIK------VSMCADKVLMD--MFDQDDDIGLVALSDEEPSSTGELRYYELV 204

  Fly   229 HQGEENTRRHEDENGFVVMV--------------TELRSIGGAGTDSAGSGGSATGASASLNMKR 279
            .......|::..|...::.|              |::.:|                .|..:::..
 Frog   205 RTEIAGERQYLRELNLIIKVFREAFLSNRKLFTPTDIETI----------------FSNIMDIHE 253

  Fly   280 GHVVIRGTPERLLQQLVEENSMTDP-----TYVEDF----------LLTHRIFIQNPQEVTSKL- 328
            ..|.:.|    |::..||....:.|     :..||.          .|:..|...|.||..:.|
 Frog   254 LTVKLLG----LIEDTVEMTDESSPHPLVGSCFEDLAEEQAFDPYETLSQDILASNYQEHFNTLM 314

  Fly   329 ------LHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLL 387
                  ||:   :.:.....:.::..:.|::|:.|          |..:.:.||.|.|.||.:..
 Frog   315 SKPSVALHF---QSIAEGFKEAVKYVLPRLMLVPV----------YHCLHYFEVLELLQERSEDQ 366

  Fly   388 SQLRLLHIACAAKARMRSCTLTR-----SSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVE 447
            .....|..|..|...:| |::.|     |.|..|
 Frog   367 EDRECLKQAITALLNLR-CSMERIFNKHSPRRRP------------------------------- 399

  Fly   448 PGSKAQDVGLKRGDQIHEVNGQSL--DHVTSKRALEILTGTTHLSISVKSNLLGF--KEIMQALE 508
                        |:.|..:..:.:  .||..|:..||           :.|:..:  |:|.|
 Frog   400 ------------GEPIWRLYNRQMRSKHVAIKKMNEI-----------QKNIDSWEGKDIGQ--- 438

  Fly   509 HGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDM 573
                                       |.|:.. :.|       .|:.|.|.:..|:       .
 Frog   439 ---------------------------CCNEFI-MEG-------SLTRVGAKHERHI-------F 461

  Fly   574 LLDQPDCAPPQTPPVSGSGNMAS---NFMQQLLQSVNNSS--------AKKSGGNSNSDQQDTKG 627
            |.|               |.|.|   |..|..:...|.:.        .:|...|...|..:.|.
 Frog   462 LFD---------------GLMISCKMNHGQSRIPGYNTAEYRLKEKLIMRKVQINDKEDTSEYKN 511

  Fly   628 GFMTLAPKRRLQKALAK--------MNLLNKQNHGSSLNDSSDTLL----NDPKSKL-------- 672
            .|..|:.........||        |..|....:.|.|:...||:|    |:...:|
 Frog   512 AFELLSKDENSIVFAAKTAEEKNNWMAALISLQYRSMLDRMLDTVLIQEENEQPLRLPSPDVYRF 576

  Fly   673 ----SAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAH 733
                |..:.....:..|.:|..:..||.:.:                          .:..|..|
 Frog   577 VEEDSEENIVFEDSVQSRSGIPIIKGGTVVK--------------------------LIERLTYH 615

  Fly   734 IHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPS 798
            ::..                |::....|..|::  .||         ..|::.|.::.|.|.||
 Frog   616 MYAD----------------PNFVRTFLTTYRS--FCK---------PQELLNLLIERFEIPDP- 652

  Fly   799 SNFSLCEVSVGDGGMVKQRRLP----------DQLQNLAERISFAARYY-----------LKLND 842
                  |.:..|...:::...|          :.:|.:..|:....|::           |:|.|
 Frog   653 ------EPTESDKMAIEKGEQPISANLKRFRKEYVQPVQLRVLNVFRHWVEHHFYDFERDLELLD 711

  Fly   843 STEPLVPD----------ELALELVRES----------NVHF----------------------L 865
            ..|..:.:          |...:::|..          |:.|                      :
 Frog   712 RLESFISNVRGKSMKKWVESIAKIIRRKKQAQANGVSHNITFESPFPPIEWHISRTGQCETFDLM 776

  Fly   866 HLNAYELAIQLTLQDFANFRQIESTEYVDELF-----ELRSRYGVPMLSKFAELVNREMFWVVSE 925
            .|:..|:|.||||.:...:|.::.:|.|..::     |:.|    |.|.|..........|....
 Frog   777 TLHPIEIARQLTLMESDLYRAVQPSELVGSVWTKEDKEINS----PNLLKMIRHTTNLTLWFEKC 837

  Fly   926 ICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFND 990
            :....|...|:.:..:.|:|.:..:|..|||.:..|||.:....|.||..|:|.|..:.:::.::
 Frog   838 LVETENFEERVAVFSRIIEILQVFQELNNFNGVLEIVSAVNSVPVYRLDHTFEALQERKKKILDE 902

  Fly   991 LQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGND--TRVDG--LINFEKLRM 1051
            ..:|     :...:::.::.......|.:||:.|...::.....||.  .:..|  ||||.|.|.
 Frog   903 AVEL-----SQDHFKKYLAKLKSINPPCVPFFGIYLTNILKTEEGNPDFLKRQGKELINFSKRRK 962

  Fly  1052 LAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRK 1116
            :|:....:....:.||.|.:                                     :|.|:|..
 Frog   963 VAEITGEIQQYQNQPYCLRT-------------------------------------EADIQRFF 990

  Fly  1117 KSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEP---------AHGSTYSGSI 1172
            ::.   ||...|.|.:..                |.|...|.|.||         :..:||
 Frog   991 ENL---NPIGNFGEKEFT----------------DYLFNKSQEIEPRNCKQPPRFSRKTTY---- 1032

  Fly  1173 SHGNTSHRSGGGGSISGGAGGSSGGGGGGSSSLN------AGDQLSIYSHTSSSSAPNSSLSLRK 1231
                 |.:|.|....||....|||...|....|.      :..:::...|.|:.|.|.|
 Frog  1033 -----SLKSPGIRPNSGRHLSSSGTLRGHPMPLEREPYKISFSRITESDHESTISPPIS------ 1086

  Fly  1232 RHPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLS-------------------L 1277
              |::|:....|::|..|......:|::   ||:::      |||                   |
 Frog  1087 --PNTPSTPPASASSELSVFVDLDLHSS---FGSST------MLSHGFLPNSKSFFSSCGSLHRL 1140

  Fly  1278 SESSKIRPHQPFVPR-----HGSTMAG---------VIPPLHHMHAAHGFSTPSPGGVVTSPATS 1328
            ||...:.|  |..||     .||.:.|         .|||          ..|.|..:  .|...
 Frog  1141 SEEPLLPP--PLPPRRKVDPEGSNLVGHSKLDDDPPAIPP----------RLPPPPKI--QPRVP 1191

  Fly  1329 AVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPP--PLPSVDLSLESSSVTTFRD 1391
            |.......|..||                          |.|||  |:|.....:.......|.:
 Frog  1192 AYTGPFDGPLHSP--------------------------PPPPPRDPIPDTPPPVPQRPPEHFIN 1230

  Fly  1392 LPLRKSVTSGSISSCDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPPVYTAADCRLLQQISNNAVT 1456
            .||       ::.....|.:|::|.:.
 Frog  1231 CPL-------NLQPPPLGRLHREQDWF-------------------------------------- 1250

  Fly  1457 RNLNSPCQSTNTPPSTPTPPPNQPTATIQLSAPPTAAAYMHARSQHQQLQQQQQSLAMPPPPPPP 1521
            |.:|:...|.|||||||:|...:....:..|         |:...|          .:.||.||.
 Frog  1251 REVNTAPNSPNTPPSTPSPRALRRCCGLSTS---------HSNLSH----------VLAPPVPPR 1296

  Fly  1522 YNVPP 1526
            .|..|
 Frog  1297 QNSNP 1301

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
PDZ-GEF	NP_609012.2	CAP_ED	128..237	CDD:237999	15/116 (13%)
		RasGEFN	279..400	CDD:214571	28/142 (20%)
		PDZ_RapGEF2_RapGEF6-like	404..496	CDD:467237	13/98 (13%)
		RA_PDZ-GEF1	759..841	CDD:340483	17/102 (17%)
		RasGEF	871..1050	CDD:459872	45/187 (24%)
sos2	XP_002938172.3	HFD_SOS1_rpt1	18..95	CDD:467039	11/58 (19%)
		HFD_SOS1_rpt2	104..176	CDD:467040	13/97 (13%)
		RhoGEF	200..382	CDD:238091	37/214 (17%)
		PH_SOS	438..544	CDD:269963	26/165 (16%)
		RasGEFN	596..740	CDD:214571	28/203 (14%)
		RasGEF	775..1014	CDD:238087	61/303 (20%)