DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pfas and PUR4

DIOPT Version :9

Sequence 1:	NP_477212.1	Gene:	Pfas / 33847	FlyBaseID:	FBgn0000052	Length:	1354	Species:	Drosophila melanogaster
Sequence 2:	NP_177566.3	Gene:	PUR4 / 843766	AraportID:	AT1G74260	Length:	1407	Species:	Arabidopsis thaliana

Alignment Length:	1375	Identity:	640/1375 - (46%)
Similarity:	857/1375 - (62%)	Gaps:	87/1375 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 ILRYYDVQAHSAAEEESVLRRLREE-DGAVVSVRMERCYHLEYSAQAEHSLALDE---LLVWLVK 63
            ::.:|.|.....:....:|:.::.: ...:||:..|:.:::...::.:     ||   :|.|:::
plant    87 VIHFYRVPLIQESANAELLKAVQTKISNQIVSLTTEQSFNIGLESKLK-----DEKLSVLKWILQ 146

  Fly    64 QP-----LSKGQSLSRQPALQSTGSSQLLLEIGPRFNFSTPYSTNCVNIFQNLGYSEVRRMETST 123
            :.     |.....|.|:   :..|...:::|:|||.:|:|.:|||.|:|.:..|..||.|:|.|.
plant   147 ETYEPENLGTDSFLERK---KQEGLHAVIVEVGPRLSFTTAWSTNAVSICRACGLDEVTRLERSR 208

  Fly   124 RYLVTFGEGSKAPEAARFVPLLGDRMTQCLYTEENTPKASFDEQLPERQANWHFVPVLEEGRAAL 188
            |||:...|.....:...|..::.||||:|:||::   ..||:..:...:..  :|||:|:||.||
plant   209 RYLLFSKEPLLENQIKEFAAMVHDRMTECVYTQK---LVSFETNVVPEEVK--YVPVMEKGRKAL 268

  Fly   189 ERINQELGLAFNDYDLDYYHDLFAKELGRNPTTVELFDCAQSNSEHSRHWFFRGRMVIDGVEQPK 253
            |.||||:||||::.||.||..||.:::.|:||.|||||.|||||||||||||.|.|||||....|
plant   269 EEINQEMGLAFDEQDLQYYTRLFREDIKRDPTNVELFDIAQSNSEHSRHWFFAGNMVIDGKPMDK 333

  Fly   254 SLIRMIMDTQAHTNPNNTIKFSDNSSAMVGFDHQTIVPSSVVAPGAVRLQSVQS---DLIFTAET 315
            ||::::..|......|:.|.|.|||||:.||....:.|   :.||:|.|..|.:   |::|||||
plant   334 SLMQIVKSTWEANRNNSVIGFKDNSSAIRGFLVNQLRP---LLPGSVCLLDVSARDLDILFTAET 395

  Fly   316 HNMPTAVAPFSGATTGTGGRLRDVQGVGRGGVPIAGTAGYCVGALHIPGYKQPYEPLDFKYPATF 380
            ||.|.||||:.||.||.|||:||....|||...:|.|:|||||.|::.|...|:|...|:||:..
plant   396 HNFPCAVAPYPGAETGAGGRIRDTHATGRGSFVVASTSGYCVGNLNMEGSYAPWEDSSFQYPSNL 460

  Fly   381 APPLQVLIEASNGASDYGNKFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMR 445
            |.|||:||:|||||||||||||||:|.|:..::|:.  ..:..|.|::||||||.|:|.:..|..
plant   461 ASPLQILIDASNGASDYGNKFGEPMIQGYTRTFGMR--LPSGDRREWLKPIMFSAGIGQIDHTHI 523

  Fly   446 EKLPPARGQLLAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACL 510
            .|..|..|.|:.|||||.||||:|||||||: :.|..||||||||||||||||..||.||||||:
plant   524 TKGEPEVGMLVVKIGGPAYRIGMGGGAASSM-VSGQNDAELDFNAVQRGDAEMSQKLYRVVRACI 587

  Fly   511 DLGEQNPILAIHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAIL 575
            ::||:|||::|||||||||.||:||::.|  .||.|..:...:||.|::.||:|||||||.:|||
plant   588 EMGEKNPIISIHDQGAGGNCNVVKEIIYP--QGAEIDIRAVVVGDHTMSVLEIWGAEYQEQDAIL 650

  Fly   576 CNADQRELLEKICRRERCPISFVGVVTGDGRVTLLEKPAPKDLEQALNASNRSEVSP---FDLEL 637
            ..|:.||:|:.||:|||..::.:|.:.|.||.||::..|      |...|......|   .||||
plant   651 VKAESREILQSICKRERLSMAVIGTINGGGRCTLIDSTA------AAKCSKEGLPPPPPAVDLEL 709

  Fly   638 KYVLGDMPKRTYDLKREQTPLKELSLPKGLLLDEALERVLSLVAVGSKRFLTNKVDRCVGGLIAQ 702
            :.|||||||:|:...|.....:.|.:..|:.|.:||:|||.|.:|.||||||.||||||.||:||
plant   710 EKVLGDMPKKTFKFNRIAYAREPLDIAPGITLMDALKRVLRLPSVSSKRFLTTKVDRCVTGLVAQ 774

  Fly   703 QQCVGPLQAPLADYALTTVSHFSHSGIATSIGTQPLKGLLDPAAMARMCVAEALSNLVFVKISEL 767
            ||.|||||..|||.|:...:....:|.|.:||.||:||||||.||||:.|.|||:|||:.|::.|
plant   775 QQTVGPLQITLADVAVIAQTFTDLTGGACAIGEQPIKGLLDPKAMARLAVGEALTNLVWAKVTAL 839

  Fly   768 ADVKCSGNWMWAAKLPGEGARMFDACKELCQILEELHIAIDGGKDSLSMAAKVGGETIKSPGTLV 832
            :|||.|||||:||||.|||:.|:||...|.:.:.||.||||||||||||||...||.:|:||.||
plant   840 SDVKASGNWMYAAKLEGEGSAMYDAAIALSEAMIELGIAIDGGKDSLSMAAHADGEVVKAPGNLV 904

  Fly   833 ISTYAPCPDVRLKVTPDLKGPGAGSKTSLLWINL-ENSARLGGSALAQAYAQQGKDTPNLTRSDV 896
            ||.|..|||:...||||||  ..|....||.::| :...|||||||||.:.|.|.|.|:|.....
plant   905 ISAYVTCPDITKTVTPDLK--LGGDDGILLHVDLAKGKRRLGGSALAQVFGQIGNDCPDLDDVPY 967

  Fly   897 LGKAFAVTQSLLGDGLIQAGHDVSDGGLLVCVLEMAIGGLSGLRVDLSEPLAKLKNFDKSVEKLN 961
            |...|...|:|:.:.|:.||||:|||||:|..||||..|..|:.:||:.....|
plant   968 LKNVFDGVQALIAENLVSAGHDISDGGLVVTALEMAFAGNKGINLDLASNGISL----------- 1021

  Fly   962 RPELAVLFAEECGWVVEVLDTDLERVRSTYEKAGVPNYYLG-VTEGFGLDSRVVLKNGKSELLDQ 1025
               ...||:||.|.|:|:..|:|:.|........|....:| ||:...::.:|   :|.:. |.:
plant  1022 ---FETLFSEELGLVLEISKTNLDAVMEKLRAFDVTAEIIGNVTDSPLIEVKV---DGITH-LSE 1079

  Fly  1026 PLRVLYKKWERTSYELEKLQANPECAEAEYNSLEYRQAPQYRGPQNVQAELTLKRSS-------- 1082
            ....|...||.||::|||||....|.|.|...|::|..|.::        |:...||
plant  1080 KTSFLRDMWEDTSFQLEKLQRLASCVEMEKEGLKFRHEPNWK--------LSFIPSSTNNNYMSQ 1136

  Fly  1083 -APVRVAVLREEGVNSEREMMACLLRANFEVHDVTMSDLLQGTASVSQYRGLIFPGGFSYADTLG 1146
             ...:|||:||||.|.:|||.|....|.||..|||:||||.|..::.|:||::|.|||||||.|.
plant  1137 DVKPKVAVIREEGSNGDREMSAAFYAAGFEPWDVTVSDLLAGDITLDQFRGIVFVGGFSYADVLD 1201

  Fly  1147 SAKGWAANILHNPRLLPQFEAFKRRQDVFSLGICNGCQLMTLIGFVGSAKSEVGADPDVA---LL 1208
            |||||||:|..|..:|.||:.|.:|.|.||||||||||||.|:|:|..  .:||...|.:   .:
plant  1202 SAKGWAASIRFNEPVLSQFQEFYKRPDTFSLGICNGCQLMALLGWVPG--PQVGGSLDTSQPRFV 1264

  Fly  1209 HNKSQRFECRWATVKIPSNRSIMLGSMKDLVLGCWVAHGEGRFAFRDEKLISHLQSEQLVTLQYV 1273
            ||:|.|||||:.:|.|..:.||||..|:...||.|.||||||..|.||.::.|:....|..|:|.
plant  1265 HNESGRFECRFTSVTIKDSPSIMLKGMEGSTLGVWAAHGEGRAYFPDEGVLDHMLHSDLAPLRYC 1329

  Fly  1274 DDVGKPTELYPLNPNGSPQGIAGLCSSDGRHLALMPHPERCSSMYQWPYVPSSFEVSPTQSESPW 1338
            ||.|..||.||.|.||||.|||.:||.||||||:|||||||..|:|:|:.|:|::|... ..|||
plant  1330 DDDGNVTEAYPFNLNGSPLGIAAICSPDGRHLAMMPHPERCFLMWQFPWYPTSWDVEKA-GPSPW 1393

  Fly  1339 QIMFNNAYNW 1348
            ..||.||.:|
plant  1394 LKMFQNARDW 1403

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pfas	NP_477212.1	PLN03206	18..1349	CDD:178745	638/1360 (47%)
		PurL_repeat1	223..612	CDD:100034	214/391 (55%)
		PurL_repeat2	715..1004	CDD:100035	135/290 (47%)
		GATase_5	1085..1349	CDD:290241	143/267 (54%)
PUR4	NP_177566.3	PLN03206	102..1405	CDD:178745	638/1360 (47%)
		PurL_repeat1	303..686	CDD:100034	214/390 (55%)
		PurL_repeat2	787..1061	CDD:100035	135/289 (47%)
		GATase_5	1139..1404	CDD:290241	143/268 (53%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	284	1.000	Domainoid score	I383
eggNOG	1	0.900	-	-		E1_COG0046
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H5970
Inparanoid	1	1.050	1130	1.000	Inparanoid score	I25
OMA	1	1.010	-	-		QHG55304
OrthoDB	1	1.010	-	-		D38224at2759
OrthoFinder	1	1.000	-	-		FOG0004359
OrthoInspector	1	1.000	-	-		oto3400
orthoMCL	1	0.900	-	-		OOG6_101332
Panther	1	1.100	-	-	LDO	PTHR10099
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3082
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.840