DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pfas and PFAS

DIOPT Version :9

Sequence 1:	NP_477212.1	Gene:	Pfas / 33847	FlyBaseID:	FBgn0000052	Length:	1354	Species:	Drosophila melanogaster
Sequence 2:	NP_036525.1	Gene:	PFAS / 5198	HGNCID:	8863	Length:	1338	Species:	Homo sapiens

Alignment Length:	1377	Identity:	682/1377 - (49%)
Similarity:	890/1377 - (64%)	Gaps:	69/1377 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 ILRYY-DVQAHSAAEEESVLRRLREEDGAVVSVRMERCYHLEYSAQAEHSLALDELLVWLVKQPL 66
            :|.:| ....|..|......|:|:.:...:..|..|.||::.::|:|..|....:.|:||...||
Human     4 VLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPL 68

  Fly    67 SKGQSLSRQPALQSTGSSQLLLEIGPRFNFSTPYSTNCVNIFQNLGYSEVRRMETSTRYLVTFGE 131
            .. ..::|:..| ..||:.||||:|||.|||||.|||.|::.:..|...|.|:||:.||.::|..
Human    69 LL-DDVARESWL-LPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAH 131

  Fly   132 GSKAPEAARFVPLLGDRMTQCLYTEENTPKASFD-EQLPERQANWHFVPVLEEGRAALERINQEL 195
            ...|...|..:..|.||||:..:..   |..||. |.:|| ..|.. :.:|.|||.|||:.||||
Human   132 PPSAEVEAIALATLHDRMTEQHFPH---PIQSFSPESMPE-PLNGP-INILGEGRLALEKANQEL 191

  Fly   196 GLAFNDYDLDYYHDLFAKELGRNPTTVELFDCAQSNSEHSRHWFFRGRMVIDGVEQPKSLIRMIM 260
            |||.:.:|||:|...| :||.|||:|||.||.|||||||||||||:|::.:||.:...||...||
Human   192 GLALDSWDLDFYTKRF-QELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIM 255

  Fly   261 DTQAHTNPNNTIKFSDNSSAMVGFDHQTIVPSSVVAPGAVRLQSVQSDLIFTAETHNMPTAVAPF 325
            .||..:||||.:||.|||||:.|.:.:.:.|.....|...:.|.....::|||||||.||.|.||
Human   256 STQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPF 320

  Fly   326 SGATTGTGGRLRDVQGVGRGGVPIAGTAGYCVGALHIPGYKQPYEPLDFKYPATFAPPLQVLIEA 390
            ||||||||||:||||..|||...:|||||||.|.||||||..|:|...|:||..||.||:|.|||
Human   321 SGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEA 385

  Fly   391 SNGASDYGNKFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMREKLPPARGQL 455
            |||||||||||||||::|||.|.||.  ....||.|::||||||||:|:|.|....|..|..|..
Human   386 SNGASDYGNKFGEPVLAGFARSLGLQ--LPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGME 448

  Fly   456 LAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACLDLGEQNPILA 520
            :.|:|||||||||||||||||::||...::|||.||||||.|||.|:|||:|||::..:.|||.:
Human   449 VVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICS 513

  Fly   521 IHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAILCNADQRELLE 585
            :|||||||||||||||.:|  |||:|::..|||||||:.|||:|||||||:||:|..:..|:.|.
Human   514 LHDQGAGGNGNVLKELSDP--AGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLT 576

  Fly   586 KICRRERCPISFVGVVTGDGRVTLLEKPAPKDLEQALNASNRSEV------SPFDLELKYVLGDM 644
            .:..|||||..|||.:|||.|:.|::     |.|..:..:.:.:.      :|.||||::|||.|
Human   577 HVSARERCPACFVGTITGDRRIVLVD-----DRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKM 636

  Fly   645 PKRTYDLKREQTPLKELSLPKGLLLDEALERVLSLVAVGSKRFLTNKVDRCVGGLIAQQQCVGPL 709
            |::.:.|:|:...|:.|:||.||.:.:||||||.|.||.|||:|||||||.||||:|||||||||
Human   637 PRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPL 701

  Fly   710 QAPLADYALTTVSHFSHSGIATSIGTQPLKGLLDPAAMARMCVAEALSNLVFVKISELADVKCSG 774
            |.||||.|:..:||....|.||::|.||:|.||||...||:.|||||:||||..:::|.||||||
Human   702 QTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSG 766

  Fly   775 NWMWAAKLPGEGARMFDACKELCQILEELHIAIDGGKDSLSMAAKVGGETIKSPGTLVISTYAPC 839
            |||||||||||||.:.|||:.:..::..|.:|:|||||||||||:||.||:::||:||||.||.|
Human   767 NWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVC 831

  Fly   840 PDVRLKVTPDLKGP-GAGSKTSLLWINLE-NSARLGGSALAQAYAQQGKDTPNLTRSDVLGKAFA 902
            ||:...||||||.| |.|   .||::.|. ...||||:||||.::|.|:..|:|...:.|.:||:
Human   832 PDITATVTPDLKHPEGRG---HLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFS 893

  Fly   903 VTQSLLGDGLIQAGHDVSDGGLLVCVLEMAIGGLSGLRVDLSEPLAKLKNFDKSVEKLNRPELAV 967
            :||.||.|.|:.:|||||||||:.|:||||..|..||:||:..|...:              |:|
Human   894 ITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDV--------------LSV 944

  Fly   968 LFAEECGWVVEVLDTDLERVRSTYEKAGVPNYYLGVTEGFGLDSRVVLKNGKSELLDQPLRVLYK 1032
            |||||.|.|:||.:.||.:|...|..||:....||.|...|..:.|.:....:.:|::|:..|..
Human   945 LFAEEPGLVLEVQEPDLAQVLKRYRDAGLHCLELGHTGEAGPHAMVRVSVNGAVVLEEPVGELRA 1009

  Fly  1033 KWERTSYELEKLQANPECAEAEYNSLEYRQAPQYRGPQNVQAELTLKRSSAP-------VRVAVL 1090
            .||.||::|::|||.|.|...|...|..|..|.|..|.      |..::|.|       .|||:|
Human  1010 LWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPP------TFPKASVPREPGGPSPRVAIL 1068

  Fly  1091 REEGVNSEREMMACLLRANFEVHDVTMSDLLQGTASVSQYRGLIFPGGFSYADTLGSAKGWAANI 1155
            ||||.|.:|||......|.|||.||||.||..|...:..:||:.|.|||||||.|||||||||.:
Human  1069 REEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAV 1133

  Fly  1156 LHNPRLLPQFEAFKRRQDVFSLGICNGCQLMTLIGFVGSAKSEVGAD---------PDVALLHNK 1211
            ..:||...:...|::|.|.||||:||||||:.|:|:||...:|..|:         |.:.|.||.
Human  1134 TFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNL 1198

  Fly  1212 SQRFECRWATVKIPSNRSIMLGSMKDLVLGCWVAHGEGRFAFRDEKLISHLQSEQLVTLQYVDDV 1276
            |.|:|.|||:|::....::||..|:..||..|.|||||..||...:|.:.:::..|..|.:.||.
Human  1199 SGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDD 1263

  Fly  1277 GKPTELYPLNPNGSPQGIAGLCSSDGRHLALMPHPERCSSMYQWPYVPSSFEVSPTQSESPWQIM 1341
            |.|||.|||||||||.|:||:||.||||||:||||||....:||.:.|..|:   |.:.|||..:
Human  1264 GNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFD---TLTTSPWLQL 1325

  Fly  1342 FNNAYNWCVKSN 1353
            |.||.||.::.:
Human  1326 FINARNWTLEGS 1337

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pfas	NP_477212.1	PLN03206	18..1349	CDD:178745	677/1355 (50%)
		PurL_repeat1	223..612	CDD:100034	231/388 (60%)
		PurL_repeat2	715..1004	CDD:100035	149/290 (51%)
		GATase_5	1085..1349	CDD:290241	134/272 (49%)
PFAS	NP_036525.1	FGAM_synt	7..1333	CDD:188163	680/1368 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165152573
Domainoid	1	1.000	285	1.000	Domainoid score	I1629
eggNOG	1	0.900	-	-		E1_COG0046
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H5970
Inparanoid	1	1.050	1245	1.000	Inparanoid score	I179
Isobase	1	0.950	-	0	Normalized mean entropy	S1981
OMA	1	1.010	-	-		QHG55304
OrthoDB	1	1.010	-	-		D38224at2759
OrthoFinder	1	1.000	-	-		FOG0004359
OrthoInspector	1	1.000	-	-		oto89268
orthoMCL	1	0.900	-	-		OOG6_101332
Panther	1	1.100	-	-	LDO	PTHR10099
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2247
SonicParanoid	1	1.000	-	-		X3082
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	17	16.750