Protein Alignment Pfas and pfas

DIOPT Version :9

• Sequence 1: NP_477212.1
• Gene: Pfas / 33847
• FlyBaseID: FBgn0000052
• Length: 1354
• Species: Drosophila melanogaster
• Sequence 2: NP_001135692.3
• Gene: pfas / 100216263
• XenbaseID: XB-GENE-6001438
• Length: 1324
• Species: Xenopus tropicalis
• Alignment Length: 1365
• Identity: 720/1365 - (52%)
• Similarity: 911/1365 - (66%)
• Gaps: 65/1365 - (4%)

  Fly     1 MVILRYYDVQAHSAAEEESVLRRLREEDGAVVSVRMERCYHLEYSAQAEHSLALDELLVWLVKQP 65
            ||:|.:|    .|.......||....|:  :.|::.|.||::.::..|..|....:.|.||...|
 Frog     1 MVVLHFY----RSLQNSSETLRLPGAEN--IQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCP 59

  Fly    66 LSKGQSLSRQPALQSTGSSQLLLEIGPRFNFSTPYSTNCVNIFQNLGYSEVRRMETSTRYLVTFG 130
            ... ||:|...:|.|. .|.||:|||||.||||..|||.|:|.:::|.:.|.|:|.|.|||:.|.
 Frog    60 FDP-QSISDTSSLHSE-PSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQ 122

  Fly   131 EGSKAPEAARFVPLLGDRMTQCLYTEENTPKASFDEQL-PERQANWHFVPVLEEGRAALERINQE 194
            ...:..|....:..|.||||:|:|.|   |..||:..: ||:.   :.|.:|.:||||||:.|.|
 Frog   123 TRPQGSEEQELLASLYDRMTECVYPE---PVRSFEVSVRPEKV---YEVDILGQGRAALEKANTE 181

  Fly   195 LGLAFNDYDLDYYHDLFAKELGRNPTTVELFDCAQSNSEHSRHWFFRGRMVIDGVEQPKSLIRMI 259
            |||||:.:|||||..|| :.:||||::||.||.|||||||||||||:|::.:||.|:..||..||
 Frog   182 LGLAFDSWDLDYYSSLF-QRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMI 245

  Fly   260 MDTQAHTNPNNTIKFSDNSSAMVGFDHQTIVPSSVVAPGAVRLQSVQSDLIFTAETHNMPTAVAP 324
            |.||..:||||.|||.|||||:.|.:..:::|:.....|...|......|||||||||.||.|||
 Frog   246 MKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAP 310

  Fly   325 FSGATTGTGGRLRDVQGVGRGGVPIAGTAGYCVGALHIPGYKQPYEPLDFKYPATFAPPLQVLIE 389
            |||||||||||:||||..|||...||||||||.|.||||||..|:|.|.::||..||.||:|.||
 Frog   311 FSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDLAYQYPVQFARPLEVAIE 375

  Fly   390 ASNGASDYGNKFGEPVISGFALSYGLNSAADASQRDEYVKPIMFSGGLGTMPATMREKLPPARGQ 454
            ||||||||||||||||::|||.|:||.  ..:.:|.|:|||||||||:|:|....|.|..|..|.
 Frog   376 ASNGASDYGNKFGEPVLAGFARSFGLR--LPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGM 438

  Fly   455 LLAKIGGPVYRIGVGGGAASSVEIQGSGDAELDFNAVQRGDAEMENKLNRVVRACLDLGEQNPIL 519
            .:.|:||||||||||||||||:::||...:||||.||||||||||.|:||.||||::.|.:|||.
 Frog   439 HVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPIC 503

  Fly   520 AIHDQGAGGNGNVLKELVEPGFAGAVIFSKEFQLGDPTITALELWGAEYQENNAILCNADQRELL 584
            :||||||||||||||||.||  .||||::|.|||||||::.||:|||||||:||:|...|..|.|
 Frog   504 SIHDQGAGGNGNVLKELSEP--QGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFL 566

  Fly   585 EKICRRERCPISFVGVVTGDGRVTLLEKPAPKDLEQALNASNRSEVSPFDLELKYVLGDMPKRTY 649
            ..:|||||.|:.|||.:|||||:.|:.   ..|.:.|.::::|:.| |.||:|::|||.||::.:
 Frog   567 RSVCRRERSPVDFVGKITGDGRIVLIN---GSDTDPAPDSTDRNAV-PVDLQLEWVLGKMPRKEF 627

  Fly   650 DLKREQTPLKELSLPKGLLLDEALERVLSLVAVGSKRFLTNKVDRCVGGLIAQQQCVGPLQAPLA 714
            .|.|....|:.|.||.||.:.:||:|||.|.:|.|||:|||||||.|.||:|||||||||..|||
 Frog   628 VLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLA 692

  Fly   715 DYALTTVSHFSHSGIATSIGTQPLKGLLDPAAMARMCVAEALSNLVFVKISELADVKCSGNWMWA 779
            |.|:.::|:....|.||:||.||:|.||:|||.||:.|||||:||:|.::::|.|||||||||||
 Frog   693 DVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWA 757

  Fly   780 AKLPGEGARMFDACKELCQILEELHIAIDGGKDSLSMAAKVGGETIKSPGTLVISTYAPCPDVRL 844
            ||||||||.::|||..:|.::.:|.:|||||||||||||:||.||:|:||:||||.||.|||:..
 Frog   758 AKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITT 822

  Fly   845 KVTPDLKGPGAGSKTSLLWINL-ENSARLGGSALAQAYAQQGKDTPNLTRSDVLGKAFAVTQSLL 908
            .||||||.|  |.|..||::.| ....|||||||||.|:|.|:..|:|.....|...|.|||.||
 Frog   823 TVTPDLKNP--GQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLL 885

  Fly   909 GDGLIQAGHDVSDGGLLVCVLEMAIGGLSGLRVDLSEPLAKLKNFDKSVEKLNRPELAVLFAEEC 973
            .|.::.||||||||||:.|:||||..|..||.:::|.....:              |.:|||||.
 Frog   886 RDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNV--------------LELLFAEEP 936

  Fly   974 GWVVEVLDTDLERVRSTYEKAGVPNYYLGVTEGFGLDSRVVLKNGKSELLDQPLRVLYKKWERTS 1038
            |.|:||.:..:|.|...|..|||....:|.|:..|..|.|.::....|::::.|..|...||.||
 Frog   937 GLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETS 1001

  Fly  1039 YELEKLQANPECAEAEYNSLEYRQAPQYRGPQNVQAELTLKRSSAPV----------RVAVLREE 1093
            ::||:|||||.|...|...|..|:.|.|        .||...|..|:          ||||:|||
 Frog  1002 FQLERLQANPSCVSQEEAGLRVREGPSY--------HLTFNPSEIPLVPPSVGGNQPRVAVVREE 1058

  Fly  1094 GVNSEREMMACLLRANFEVHDVTMSDLLQGTASVSQYRGLIFPGGFSYADTLGSAKGWAANILHN 1158
            |.|.:|||.|.||.|.|:|.||||.|||.|..::..:|||:|.|||||||.|||||||||::..|
 Frog  1059 GSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFN 1123

  Fly  1159 PRLLPQFEAFKRRQDVFSLGICNGCQLMTLIGFVG--SAKSEVGADP--DVALLHNKSQRFECRW 1219
            ..:..|||.|:||.|.||||:|||||||.|:|:||  :....||..|  .|.|.||.|.|||.|:
 Frog  1124 ASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTVGDLPTQGVLLSHNLSGRFESRF 1188

  Fly  1220 ATVKIPSNRSIMLGSMKDLVLGCWVAHGEGRFAFRDEKLISHLQSEQLVTLQYVDDVGKPTELYP 1284
            .|:||..:.||:|..|....||.|||||||...||..|:..::.|..|..|.||||.|||||.||
 Frog  1189 VTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYP 1253

  Fly  1285 LNPNGSPQGIAGLCSSDGRHLALMPHPERCSSMYQWPYVPSSFEVSPTQSESPWQIMFNNAYNWC 1349
            :||||||.|||||||:||||||:|||||||...:|||::|.|:..|  ...|||..:|.|.|.||
 Frog  1254 MNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHS--LDVSPWMRLFQNGYRWC 1316

  Fly  1350  1349
             Frog  1317  1316

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pfas	NP_477212.1	PLN03206	18..1349	CDD:178745	713/1346 (53%)
		PurL_repeat1	223..612	CDD:100034	245/388 (63%)
		PurL_repeat2	715..1004	CDD:100035	150/289 (52%)
		GATase_5	1085..1349	CDD:290241	152/277 (55%)
pfas	NP_001135692.3	PurL1	24..1317	CDD:330252	712/1338 (53%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	305	1.000	Domainoid score	I1365
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H5970
Inparanoid	1	1.050	1326	1.000	Inparanoid score	I151
OMA	1	1.010	-	-		QHG55304
OrthoDB	1	1.010	-	-		D38224at2759
OrthoFinder	1	1.000	-	-		FOG0004359
OrthoInspector	1	1.000	-	-		oto103103
Panther	1	1.100	-	-	LDO	PTHR10099
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X3082
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	11.080