Protein Alignment APOB and cv-d

DIOPT Version :10

• Sequence 1: NP_000375.3
• Gene: APOB / 338
• HGNCID: 603
• Length: 4563
• Species: Homo sapiens
• Sequence 2: NP_732076.1
• Gene: cv-d / 41921
• FlyBaseID: FBgn0265048
• Length: 1470
• Species: Drosophila melanogaster
• Alignment Length: 1755
• Identity: 324/1755 - (18%)
• Similarity: 585/1755 - (33%)
• Gaps: 590/1755 - (33%)

Human   829 YIFM----ENAFELPTGAGLQLQISSSGVIAPGAK---------------AGVKLEVANMQAELV 874
            |:|:    .:||.: .|...|:.....|.:..|||               ...||.: ..|:|||
  Fly    11 YLFLIFDAGHAFSI-IGLNKQMLYEYEGNVLVGAKPQDEGHQAPPTTGWIVRGKLTL-QRQSELV 73

Human   875 AKPSVSVEFVT--NMG-------IIIPDFARSGVQMNTNFFHESGLEAHVALKAG----KLKFII 926
            ...::.::.||  |.|       .:.|.:....:.:.     :.|..:||..|.|    .:.|
  Fly    74 LAAALVIDDVTLNNSGEKFLQNKEMYPPYKPFKIALT-----KDGAISHVVFKEGDPIWSMNF-- 131

Human   927 PSPKRPV------KLLSGG----NTLHLVSTTKTEVIPPLIENRQSWSVCKQVFPGLNYCTSGAY 981
               ||.:      ::.|.|    :.|.:..|.:||.   .:.||.::...::. |.:..|.  .|
  Fly   132 ---KRAIASVLQFQMKSSGAFVVDELGIHGTCRTEY---FVSNRTNYISIRKT-PEVKTCK--PY 187

Human   982 SNASSTDSASYYPLTGDTRLELELRPTGEIEQYSV----SATYELQREDRALVDTLKFVTQAEGA 1042
            |.|..|..::..|.|    .|.:       .|.||    .|.|.:...:    :|..:::.|. |
  Fly   188 SEAVHTTRSNVPPNT----CEFD-------HQKSVIIGNEAIYGMSPHN----ETGYYLSMAH-A 236

Human  1043 KQTEATMTFKYNRQSMTLSSEV------QIP-DFDVDLGTILRVN-----------DESTEGKTS 1089
            |.|....||:...::..::||:      :.| |..:|:.|.:...           ::.|.|::.
  Fly   237 KGTTLIHTFESTGEAQFINSELLLNFLNETPIDNPIDIETSMAAEPSNLELQRLDPNDPTGGRSP 301

Human  1090 YRLTLDIQNKKITEVALMGHLSCDTKEERKIKGVISIPRLQAEARSEILAHWSPAKLLLQMDSSA 1154
            .:     |...|.:...:.....:..|..:.|  .|.|  .....|:::      |||.:||..:
  Fly   302 QQ-----QETLIAQAGTLLDSLAEALETTEFK--FSEP--YDSTLSDVI------KLLSEMDFDS 351

Human  1155 TAYGSTVSKRVAWHYDEEKIEFEWNTGTNVDTKKMTSNFPVDLSDYPKSLHMYANRLLDHRVPQT 1219
            ..                |:..|.:.||:...:.:.:.|    .:....:...|:..|.|.:...
  Fly   352 LT----------------KLYREVDIGTSYRQETIRNIF----HEIIPRIGTKASVFLTHHLVLN 396

Human  1220 DMTFRHVGSKLIVAMSSWLQKASGSLPYTQTLQDHLN-----------SLKEFN--LQNM----G 1267
            .:|...:..:|::.|...:.:.|..|  .|..:|.||           ::..|.  :.|:    |
  Fly   397 KLTKPQIAVQLLIPMPFHIFELSAEL--VQKCEDFLNIGPDRPDVRQAAILSFATLIHNVYVAKG 459

Human  1268 LPDFHIPE------NLFLKS---DGRVKYTLNKNSLKIEIPLPFGGKSSRDLKMLETVRTPALHF 1323
            :......|      |.:|..   |.::.|....|:|::       |..:..|:.:  |:.|..|
  Fly   460 IDKEKFEEYVQKYFNAYLSDRDFDQKMLYLQGLNNLQL-------GNVANYLEPI--VQDPNEH- 514

Human  1324 KSVGFHLPSREFQVPTFTI-------PKLYQLQVPL---------LGVLDLSTNVYSNLYNWSAS 1372
                   ...:||....|:       .::|::..|:         |.|..::..:.||
  Fly   515 -------EDLKFQAAWTTLALADRRAERIYEVYWPIFESRNASLELRVAAVTLLLISN------- 565

Human  1373 YSGGNTSTDHFSLRARYHMKAD-SVVDLLSYNVQGSGETTYDHKNTFTLSCDGSLRHKFLDSNIK 1436
                .|:....|:......:.| .:::.....|....||||        .|...||..     :.
  Fly   566 ----PTAARLISIHRIIQSETDPHMINYYRTTVTSISETTY--------PCYQHLRRL-----LS 613

Human  1437 FSHVEKLGNNPVSK----GLLIFDASSSWGPQMSASVHLDSK-----KKQHLFVKEVKIDGQFRV 1492
            :.| ..|...|.|:    |..|||             :.|||     ..|...|.:.|.|  ..|
  Fly   614 YMH-RHLPQKPESRYWVTGNYIFD-------------YRDSKFGIGAMLQVFLVGDPKSD--MPV 662

Human  1493 SSFYAKGTYGLSCQRDPNTGRLNGESNLRFNSSYLQGT--NQITGRYEDGTLSLTS----TSDLQ 1551
            .:|:...|..|        |:..|:..|...:..|..|  |::..|......:..|    .:.||
  Fly   663 VAFFKFDTEAL--------GKFTGQLALYIKARGLPDTILNKMQSRNGSDPFTFKSIKALLAMLQ 719

Human  1552 SGIIKNTASLKYENYELTLKSDTNGKYKNFATSNKM------DMTFSKQNALLRSEYQADYESLR 1610
            :.|| |:..|..| :.|.::..|   ..::..:.:|      |....:...::|::...:.:::|
  Fly   720 APII-NSKDLHLE-FILQMEGKT---VLSYYLNQRMFRQLTYDNILERMQQIIRTDSHINMQTVR 779

Human  1611 F-----FSLLSGSLNSHGLELNADILG------TDKINSGAHKATLRIGQDGISTSATTNLKCS- 1663
            :     :::.:....|..:.|...:|.      |::.||...|.||.|.....|.::..:...:
  Fly   780 WPFMNRYTVPTVLGTSSDVLLQTTVLTSLRGNITEQRNSPITKHTLEIDARYSSYASVRSRSYNP 844

Human  1664 LLVLENELNAELGL--------------SGASMKLTTNGRFREHNAKFSLDGKAA------LTEL 1708
            .|.|::|:|.|.|.              ||:..:..:..|.:...:..|...:|.      :|:.
  Fly   845 FLNLDHEINREQGFLIYIPFSSELHLNESGSKCRRYSFSRPQNLTSGLSFKSRAVTKTRGLITKT 909

Human  1709 SLGSAYQAMILGVDSKN----IFNFKVSQEGLKLSNDMMGSYAEMKFDHTNSLNIAGLSLDFSSK 1769
            :.....:.|:  .:.:|    :|::.::..|::|
  Fly   910 AAAPFEEIMV--PEGRNDVVQLFSYPMTDLGVRL------------------------------- 941

Human  1770 LDNIYSSDKFYKQTVNLQLQPYSLVTTLNSDLKYNAL----DLTNN---GKLRLEPLKLHVAG-- 1825
                                  |:.|.||..:||..:    :.|.|   |.:.:..| :::.|
  Fly   942 ----------------------SMTTNLNELIKYRGMLLKSEFTENGFSGNMVVNAL-MYIFGFT 983

Human  1826 ---NLKGAYQNNEIKHIYAISSAALSASYKADTVAKVQ-------GVEFSHRLNTDIAGLASAID 1880
               ::...:..|....:|...:..:..::.|:.|.|..       |:...|   ||        .
  Fly   984 QLSSIHLGHDRNFTMLMYNEKNTRIEGNFCAEDVLKTSDMKGKQIGLTLEH---TD--------H 1037

Human  1881 MSTNYNSDSLHFSNVFRSVMAPFTMTIDAHTNGNGKLALWGEHTGQLY--SK---------FLLK 1934
            |:.|:.:|:||..|:        |:.:.|.|..|     |.:.|||:.  ||         ..|.
  Fly  1038 MNENHAADALHRWNI--------TLDVLASTKSN-----WFKLTGQVQRNSKDDEDDWKACTKLT 1089

Human  1935 AEPLAFT-FSHDYKGSTSHHLVSRKS-------------ISAALEH--------KVSALLT---P 1974
            .|||.|| ..|...|.....|.:.:|             .:...||        |:|...|   |
  Fly  1090 YEPLVFTKRPHTLNGDVVFGLATEESECPEKGSTVQFAARAGPSEHARAFLRSDKISLTDTDFCP 1154

Human  1975 AE-------QTGTWKLKTQFNN----NEYSQDLDAYNTKDKIGVELTGRTLADLTLLDSPIKVPL 2028
            .|       .|..:..::.|.|    .:|..||...|.  ....||....|..|....|..||..
  Fly  1155 KEVLKFSPIPTSRYCKRSNFENFTSITQYDMDLKFDNM--PAWFELWSNRLDHLVSALSADKVDS 1217

Human  2029 L-LSEPINIIDALEMRDAVEKPQ-EFTI---VAFVKYDKNQDVHSINLPFFETLQEYFERNRQTI 2088
            | :|:.|||        :::.|| :|.:   |..||:..:|      :|||..|...|:.:.:
  Fly  1218 LHMSQEINI--------SMQTPQDQFRLAVEVNGVKWRFHQ------IPFFYKLDSKFDASHE-- 1266

Human  2089 IVVLENVQRNLKHINIDQFVRKYRAALGKLPQQANDYLNSFNWERQVSHAKEKLTALTKKYR--- 2150
                         :..|..:::..:.:..:....:|||.:.   |:::...:.||.|.....
  Fly  1267 -------------LTFDSGLKRSCSVINGIVNTFDDYLINL---REIAVRPDCLTLLVADCSPLP 1315

Human  2151 -----ITENDIQ---------IALDDAKINFNEKL--SQLQTYMIQFDQYIKDSYDLHDLKIAIA 2199
                 :|.:.:|         :.:.....||..:.  |.|.|
  Fly  1316 QIAVFVTPSPVQGLSTNYGLRVHIGQNYFNFRARTDNSSLPT----------------------- 1357

Human  2200 NIIDEIIEKLKSLDEHYHIRVNLVKTIHDLHLFIENIDFNKSGSSTASW-IQNVDTKYQIRIQIQ 2263
                         ||...|.:|..:|.|::.            .....| |:..|  |..|:::.
  Fly  1358 -------------DEPVLIYLNQDQTPHNVR------------KKPYQWPIETSD--YDFRVELN 1395

Human  2264 EKLQQLKRHIQNIDIQHLAGKLKQHIEAIDVRVLLDQLGTTISFERIN----DILEHVKHFVINL 2324
            |         |||.|                 |...||.:||.|:..|    :|....||.:..|
  Fly  1396 E---------QNILI-----------------VECTQLSSTIQFDLYNILNFEIYGVYKHQMCGL 1434

Human  2325  2324
              Fly  1435  1434

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
APOB	NP_000375.3	Heparin-binding	32..126
		Vitellogenin_N	46..597	CDD:460170
		Heparin-binding	232..306
		Vit_open_b-sht	632..937	CDD:462702	29/145 (20%)
		Heparin-binding	902..959		14/70 (20%)
		DUF1081	960..1071	CDD:461916	26/121 (21%)
		OM_channels	1448..>1865	CDD:473880	82/492 (17%)
		Heparin-binding	2043..2178		27/157 (17%)
		235kDa-fam	<2060..>2490	CDD:130673	48/289 (17%)
		Heparin-binding	3161..3236
		Basic (possible receptor binding region)	3174..3184
		LDL receptor binding	3373..3393
		Heparin-binding	3383..3516
		Basic (possible receptor binding region)	3386..3394
		ApoB100_C	4494..4550	CDD:463606
cv-d	NP_732076.1	LPD_N	28..589	CDD:214755	117/658 (18%)
		VWD	1277..1435	CDD:214566	41/237 (17%)