Protein Alignment APOB and vit-3

DIOPT Version :10

• Sequence 1: NP_000375.3
• Gene: APOB / 338
• HGNCID: 603
• Length: 4563
• Species: Homo sapiens
• Sequence 2: NP_001367903.1
• Gene: vit-3 / 24104675
• WormBaseID: WBGene00006927
• Length: 1603
• Species: Caenorhabditis elegans
• Alignment Length: 1832
• Identity: 346/1832 - (18%)
• Similarity: 618/1832 - (33%)
• Gaps: 565/1832 - (30%)

Human     9 LALLALPALLLLLLAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSA 73
            ||:.|.|||                        |.| |....:|.|.::....||:|.|....|.
 Worm    12 LAIAASPAL------------------------DRT-FSPKSEYVYKFDGLLLSGLPTTFSDASQ 51

Human    74 TRINCKVELEVP-------QLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSE--EFAAAMSRY 129
            |.|:|:..|:..       ||.......|.....|.:   |:.|:|.::..:.|  |......|.
 Worm    52 TLISCRTRLQAVDDRYIHLQLIDIQYSASHIPQSEQW---PKIKSLEQRELSDELKELLELPFRA 113

Human   130 ELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLV------------PPETEEAKQVLFLD-- 180
            :::..:....|.    ..::..:..|.||.|::...:            ..:.|..|..||.:
 Worm   114 QIRNGLVSEIQF----SSEDAEWSKNAKRSILNLFSLRKSAPVDEMSQDQKDMESDKDSLFFNVH 174

Human   181 --TVYGNCSTHFTV-----KT---------------------RKGNVATEISTERDLGQCDRFKP 217
              |:.|:|...:|:     ||                     |.|:...|...|   ||..:.:.
 Worm   175 EKTMEGDCEVAYTIVQEGEKTIYTKSVNFDKCITRPETAYGLRFGSECKECEKE---GQFVKPQT 236

Human   218 IRTGISPLALIKGMTRPLSTLISSSQSCQYTLDAKRKHVAEAICKEQHLFLPFSYKNK-----YG 277
            :.|          .|.....|..|.....|||:...:.|.::..:.:..|:..|..|:     .|
 Worm   237 VYT----------YTFKNEKLQESEVHSIYTLNVNGQEVVKSETRSKVTFVEESKINREIKKVSG 291

Human   278 MVAQVTQTLKLEDTPKINSRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQELKKLTISEQNI 342
            ...::..:::.|   |:..:|:.:|.:.....|::.:.....:|.:.:.:.:||      .|||.
 Worm   292 PKEEIVYSMENE---KLIEQFYQQGDQAEVNPFKAIEMEQKVEQLDEIFRQIQE------HEQNT 347

Human   343 -QRANLFNKLVTELRGLSDEAVTSLLPQLIEVSSPITLQA-----------LVQCGQPQCSTHIL 395
             :..:|..:.|...|..:.|       :|.:|.:.|..:|           |...|......|::
 Worm   348 PETVHLIARAVRMFRMCTIE-------ELKKVHTTIYTKAEKKVQLVIETTLAVAGTKNTIQHLI 405

Human   396 QWLKRVHANPLLI---------------DVVTYLVALIPEPSAQQLREIFNMARDQRSRATLYAL 445
            ...::....||..               .:...|:.|...|.:::...:       |..|.|.|.
 Worm   406 HHFEKKSITPLRAAELLKSVQETLYPSEHIADLLIQLAQSPLSEKYEPL-------RQSAWLAAG 463

Human   446 SHAVNNYHKT------NPTGTQELLDIANYLMEQIQDDCTGDEDYTY---LILRVIGNMGQTMEQ 501
            |.......||      .|...|.........|:..:     :.|.||   |.|:.:||.|..:..
 Worm   464 SVVRGFASKTQDLPLIRPASRQTKEKYVRVFMQHFR-----NADSTYEKVLALKTLGNAGIDLSV 523

Human   502 LTPELKSSILKCVQSTKPSLMIQKAAIQALRKME---PKDKDQEVLLQTFLDDASPGDKRLAAYL 563
            .      .:::.:|..:..|.|:..|:.|||.::   |: |.|:|||..:.:..:..:.|:||..
 Worm   524 Y------ELVQLIQDPRQPLSIRTEAVDALRLLKDVMPR-KIQKVLLPVYKNRQNKPELRMAALW 581

Human   564 MLMRS-PSQADINKIVQILPWEQNEQVKNFVASHIANILNSEELDIQDLKKLVKEALKESQLPTV 627
            .:|.: |.:..:..||..:..|.|:.|..|..:.:.....|.....|.|      |::.|:   |
 Worm   582 RMMHTIPEEPVLAHIVSQMENESNQHVAAFTYNVLRQFSKSTNPCYQQL------AVRCSK---V 637

Human   628 MDFRKFSRNYQLYKSVS-LPSLDP---ASAKIEGNLIFDPNNYLPKESMLKTTLTAFG------F 682
            :.|.::....|:..:.| ||..:.   :..:.:...||:.|.:|||| :..:..|.||      |
 Worm   638 LLFTRYQPQEQMLSTYSQLPLFNSEWLSGVQFDFATIFEKNAFLPKE-VQASFETVFGGNWNKYF 701

Human   683 ASADLIEIGLEGKGFE----PTLE--ALFGKQGFFPDSVNKALYWVNGQVPDGVSKVLVDHFGYT 741
            |     ::|...:.||    .|||  :|:|||
 Worm   702 A-----QVGFSQQNFEQVILKTLEKLSLYGKQ--------------------------------- 728

Human   742 KDDKHEQDMVNGIMLSVEKLIKDL------KSKEVPEARAYLRILGEELGFASLHDLQLLGKLLL 800
            .|:...:.:.:||.: :::::|.:      :..:...|.|...:..:|:.:..|
 Worm   729 SDELRSRRVQSGIQM-LQEIVKKMNIRPRVQQTDSQNAHAVFYLRYKEMDYIVL----------- 781

Human   801 MGARTLQGIPQMIGEVIRKGS----------KND--FFLH--YIFMENAFELPTGAGLQLQISSS 851
              ...::.|..::.:.:|.|.          .||  |.||  ..|.|....:||..|:.|.||..
 Worm   782 --PIDMETIDNVVEKYVRNGEFDIKSLLTFLTNDSKFELHRALFFYEAERRIPTTIGMPLTISGK 844

Human   852 --GVIAPGAKAGVKLEVANMQAELVAKPSVSVEFVTNMGIIIPDFARSGVQMNTNFFHES-GLEA 913
              .:::...|..::||....:..|...|:|:...||.|....|...:....:.:...|.. ..|:
 Worm   845 MPTILSINGKVSIELEKLGARLVLDIVPTVATTHVTEMRFWYPVIEQGVKSLQSARLHTPLRFES 909

Human   914 HVALKAGKL----KFIIPSPK--------RPVKLLSGGNTLHLVSTTKTEVIPPLIENRQSWSVC 966
            .|.||...|    ||::|..|        |||..:.               :|   :|:.|..|
 Worm   910 TVELKKNTLEITHKFVVPENKKTTVSVHTRPVAFIR---------------VP---KNQDSEYV- 955

Human   967 KQVFPGLNYCTSGAYSNASSTDSASYYPLTGDTRLELELRPTGEIEQYSVSATYELQREDRALVD 1031
                           .....|.|.|.|.::           |.||::...:....:..:...|..
 Worm   956 ---------------ETEEKTISHSQYQMS-----------TEEIDRQYETFGLRINAQGNVLSQ 994

Human  1032 -TLKFVTQAE---------GAKQTEATMTFKYNRQSMTLSSEVQI-----PDFDVDLGTILRVND 1081
             ||..|...|         ..:..|.|..........|..||::.     .:||::       |:
 Worm   995 WTLPMVLMTEQDFEFTLENKNRPVEFTARVTIGNLEKTDLSEIKFDKIFEKEFDLE-------NN 1052

Human  1082 ESTEGKTSYRLTLDIQNKKITEVALMGHLSCDTKEERKIKGVISIP------------------- 1127
            || |.:..|      .:|.|.|:          :.|:..|.:|::.
 Worm  1053 ES-ENRRQY------FHKMIREI----------QSEQGFKNLITLKLEAPQQMYWNTELRTVCDK 1100

Human  1128 -----RLQAEARSEILAH----WSPAKLLL----QMDSSATAYGSTVSKRV------AWHYDEE- 1172
                 :::.:||...:.|    |:....||    ||.||.........:.|      .|...::
 Worm  1101 WIRMCKVEMDARRSPMEHENKEWTLRTELLAARPQMPSSLRQLREQPHREVQLALNAKWGSSKKS 1165

Human  1173 KIEFEWNTGTNVDTKKMTSNFPVDLSDYPK-SLHMYANRLLDHRV-------PQTDMTFRHVGSK 1229
            :|.|......:.:.||...|...:....|: .|.:.|.||....|       |:::.||..: ..
 Worm  1166 EITFNAQLEQSTEQKKFLRNIEREYKGIPEYELLIKAARLNQVNVVSEYKLTPESEYTFSRI-FD 1229

Human  1230 LIVAMSSWLQKASGSLPYTQTLQDHLNSLKEFNLQNMGLPDFHIPENLFLKSDGRVKYTLNKNSL 1294
            ||.|.:.|                   ::.|..:||               .|.||...|:...|
 Worm  1230 LIKAYNFW-------------------TVSEKRVQN---------------EDRRVVLQLSVEPL 1260

Human  1295 KIEIPLPFGGKSSRDLKMLETVRTPALHFKSVGFHLPSREFQVPTFTIPKLYQL------QVPLL 1353
                        ||.. |..|::||....:.....:|  ...:||.....::|.      ....:
 Worm  1261 ------------SRQY-MNMTIQTPEQEVELKNVRIP--RVVLPTIARRAMFQQTWEKTGATCKV 1310

Human  1354 GVLDLSTNVYSNLYNWSASYSGGNTSTDHFSLRA-------RYHMKADSV----VDLLSYNVQGS 1407
            |..::||  :.|:.     |....|:.  :||.|       |:.:.|..:    .:||...|:..
 Worm  1311 GQSEVST--FDNVI-----YRAPLTTC--YSLVAKDCSEQPRFAVLAKKINKNSEELLVKVVRRE 1366

Human  1408 GETTY---DHKNTFTLSCDGSLRHKFLDSNIKFSHVEKLGNNPVSKGLLIFDASSSWGPQMSASV 1469
            .|...   |.|  |.:..|   ..|...:.::..::|.||:|     |::.
 Worm  1367 EEIVVKKSDDK--FLVKVD---EKKVNPTELEQYNIEILGDN-----LIVI-------------- 1407

Human  1470 HLDSKKKQHLFVKEVKIDGQFRVSSFYAKGTYGLSCQRDPNTGRLNGESNLRFNSSYLQGTNQIT 1534
                 :..|   .||:.||....::..:..:....|..   .|..:||.:..|.::
 Worm  1408 -----RLPH---GEVRFDGYTVKTNMPSVASQNQLCGL---CGNNDGERDNEFMTA--------- 1452

Human  1535 GRYEDGTLSLTSTSDLQ----SGIIKNTASLKYENYELTLKSDTNGKYKNFATSNKMDMTFSKQN 1595
            ..||        |.|::    |.::||.        |..:::|...:.||:  .||.:....|.:
 Worm  1453 DNYE--------TEDVEEFHRSYLLKNE--------ECEVENDRISEKKNY--RNKWNREEKKSD 1499

Human  1596 ALLRSEYQADYE 1607
            .:..|:|:.:|:
 Worm  1500 YVSSSDYENNYD 1511

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
APOB	NP_000375.3	Heparin-binding	32..126		23/102 (23%)
		Vitellogenin_N	46..597	CDD:460170	124/646 (19%)
		Heparin-binding	232..306		15/78 (19%)
		Vit_open_b-sht	632..937	CDD:462702	73/355 (21%)
		Heparin-binding	902..959		14/69 (20%)
		DUF1081	960..1071	CDD:461916	20/125 (16%)
		OM_channels	1448..>1865	CDD:473880	28/164 (17%)
		Heparin-binding	2043..2178
		235kDa-fam	<2060..>2490	CDD:130673
		Heparin-binding	3161..3236
		Basic (possible receptor binding region)	3174..3184
		LDL receptor binding	3373..3393
		Heparin-binding	3383..3516
		Basic (possible receptor binding region)	3386..3394
		ApoB100_C	4494..4550	CDD:463606
vit-3	NP_001367903.1	Vitellogenin_N	24..617	CDD:460170	124/647 (19%)
		Vit_open_b-sht	651..935	CDD:462702	69/336 (21%)
		VWD	1300..1454	CDD:214566	35/206 (17%)