Protein Alignment Bub1 and bub1

DIOPT Version :9

• Sequence 1: NP_001285621.1
• Gene: Bub1 / 33758
• FlyBaseID: FBgn0031696
• Length: 1099
• Species: Drosophila melanogaster
• Sequence 2: NP_588140.1
• Gene: bub1 / 2538736
• PomBaseID: SPCC1322.12c
• Length: 1044
• Species: Schizosaccharomyces pombe
• Alignment Length: 1208
• Identity: 259/1208 - (21%)
• Similarity: 427/1208 - (35%)
• Gaps: 356/1208 - (29%)

  Fly    26 PLASPDEMHGFLNDKQAWEHAISLYQG--------PDPLDHWYNYICWYEN---------HAQSD 73
            |...|.|....|.:.|    .::|:|.        .||:|.||..|.|...         :...|
pombe    16 PETKPRESKTRLEEIQ----RLALFQEELDIIEELDDPVDVWYRCIEWLLETRFLGMETVNKMLD 76

  Fly    74 PELKYRETLERCLTVYEHNDYYRQDVRLVRLWLKYIA---MQTDPLHFYQVLFQRGTGRQVAAFY 135
            ..::|   ||||.  :..||.....::|.::...|..   :|.....||| |..:|.|.::|.||
pombe    77 DAIQY---LERCR--FALNDVRHLLIQLAKIKQSYETPDELQQAAKQFYQ-LASKGIGLELALFY 135

  Fly   136 IGWAAYYESREEYKDAEAVFNLAFQEKAQSTSELQHAHTKFAYARSLFYQRQQQQQQQQQQHQQH 200
            ..:.:.....:.:|:|..||:.|...:|:....|.....:|:.|..|              |..|
pombe   136 EEYGSLLIRMQRWKEASEVFHAAVSREARPLVRLLRNAAEFSRAYDL--------------HNAH 186

  Fly   201 -------------PPQDALQQLTNYAQQQMPQSYNQHRPQPYQ-----------QNVYQQYHPQA 241
                         ||:..|      ..:.:..|....:|:.:|           ||....  |||
pombe   187 PSIHDAPYSSPFPPPRIVL------GSKPVSSSTLPSKPKSFQVFSDASSSRDSQNASDL--PQA 243

  Fly   242 QA--HQAPQPHQPAPQQQLPPEQQVPYQT--------HYQER-------PRY----EPHPATQSP 285
            ::  .:|..|:.|....: ...::|.|..        :.:||       .||    :|:.|...|
pombe   244 KSLESEANTPNLPLLYDK-SSGKRVEYSAFNFLALYENGEERSMEECRAQRYLSSIQPNTAASFP 307

  Fly   286 TAIPPSQVQQQSHYAPVAESHYAPAQQSQLPPQQTTVPQLHAQQPQQQQNGNGNPPPQQSPPVTN 350
            ..:|.:::   |.:...:.|:.:|..:                          ||..:||...|.
pombe   308 KVVPKNEI---SVHHDSSSSNVSPIYK--------------------------NPVAEQSDTPTR 343

  Fly   351 EVAGLRLPRNFHAYGRNNHETWKPALTLEEPDDPSRVCHYAKQLVYPPGAGVEYSPEEILARKFK 415
            .     ||:|:....::.    .|.|.:.:...|                 |..||:
pombe   344 S-----LPKNYAYVAKST----SPELKVFDTVMP-----------------VALSPK-------- 374

  Fly   416 QLMDQKAKPSEPPEQEQQTLYD----------SYETEKSYYMTAVDGALYGQNTSSGQEN---TG 467
                ...||..|....:..|.|          |...|||           .::..|.|.:   |.
pombe   375 ----PAQKPPSPTIHTKAALADILDIFNQPLRSESLEKS-----------SKSPISAQSSYLGTP 424

  Fly   468 EEDEDNDAEEGEEEEDGGEENEEDDSDEEEEDDEEEEEHSGPYTNGVQFSAQTTFEQENRSIKIK 532
            .::::|.:..|.....|       .|.||..|.......|..|.:.:.        ..|:::...
pombe   425 LKNDENSSNSGATSLTG-------RSQEEHLDFIPSLTPSKNYPSKIY--------SPNKNLDFS 474

  Fly   533 FRKEPSSTY-SAYTIENVYQQQQQQQQEQHQIIHQPPQAVHHPSPDPAPASPIPIQRQRNGSHHH 596
            .....:.|| ::..:|||.::|                    |..:..|::   :|.:.
pombe   475 HTASKAETYKNSNELENVKREQ--------------------PFSELLPST---LQEET------ 510

  Fly   597 FHPYMLGQTSTPKSEANGYRRARTKVKRSKFQPDLCSNSNSASSVADVASSSVLAGA--PGTFND 659
                ..|.|||  :.||..||.... ..|...|........:...:.|.|:|||:.|  .|....
pombe   511 ----ATGTTST--TFANAKRRPEDS-NISPTNPKKLHTLPRSPQYSTVDSNSVLSPAMPKGYMFV 568

  Fly   660 NANFSFSSATALDNSNSSLALAVDRLNFRDTSQQQILHPVNKTLQIHNNNNNTSNNNNGTSTMAD 724
            |.|.|....:::.|                        ||           .|..:.||......
pombe   569 NENQSMKHESSVSN------------------------PV-----------ATIPHENGKHDFGQ 598

  Fly   725 FSTFQENSYFATQHD---------TEAQERRLSKA--VETIARHMDKEAIDPFNSELCRAFLAKL 778
            .|..:...:|....|         |...|..::..  :.|:...:|:...|.....|..:.|...
pombe   599 LSPIEHKPFFPKNDDELPGPSGYLTMPYEEAMASLSNLPTLINPLDQSLRDLLFQVLRPSLLRDK 663

  Fly   779 DFPGNHDAHASYKIVQTPLPKI------------SNTRTLNVLE-------GVTFSIDKEVGRGS 824
            |:..:..:.|..:.:::.:.||            ||..:|:..|       ....|:..::|:|:
pombe   664 DYHEHETSFALVEHIESFVSKIKPKAGGPGRRRSSNRHSLDGPEFHLFYPPNTNLSVISKLGQGA 728

  Fly   825 YGSVY---KATDSRTGNV------------VALKYQKPPNTWEIYICDQVLKRIKEPEVLPGVMD 874
            :..||   ...::..|:|            .|||.:.||:.:|.|:..|.:.|:|.......::.
pombe   729 FAPVYLVKSKIETENGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKGLRETNSILP 793

  Fly   875 ISTAIIAPNASLIATEFSPFGSLLDINNKIRQAT--TKVMHESLVMHFSAQICNIVDHLHRQHII 937
            :....:..:.|.:..::.|.||:||:.|.:..:|  :..|.|.||:.||.:...|::.||...||
pombe   794 VHQLHMFHDTSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIEFLRIIEALHTHKII 858

  Fly   938 HADIKPDNFLLMRVPNVD---SPLPS-----------LRLIDFGCAIDMTLFPDGEKTKFRKVVQ 988
            |.|:|.||.||......|   ||:.|           :.|||||..||::||.  ||.||.....
pombe   859 HGDLKADNALLRLETVADSEWSPIYSPEGLYGWSFKGIYLIDFGRGIDLSLFE--EKVKFIADWD 921

  Fly   989 TDGFTCIEMQEGRSWSYETDLFCIAATVHVMLFGDYMQPQKKGSSWEIR----QKLPRYLKKHVW 1049
            ||...||||:|||.|:|:.|...:||.::.||||.|::.:.:..:.:.|    |::.||..:.:|
pombe   922 TDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRIEVINGQRRQVLTQRMKRYWNQDLW 986

  Fly  1050 TKFFGDLLNMQADKLPALHEMRLIFEEEAYRMDSELQK 1087
            .:.|..|||      |.||     ..||...|..||.|
pombe   987 HRLFDLLLN------PTLH-----VSEENLPMTEELSK 1013

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Bub1	NP_001285621.1	Mad3_BUB1_I	38..158	CDD:214817	34/139 (24%)
		STKc_Bub1_BubR1	813..1096	CDD:270883	97/310 (31%)
bub1	NP_588140.1	Mad3_BUB1_I	30..158	CDD:214817	34/137 (25%)
		STKc_Bub1_BubR1	718..1040	CDD:270883	97/309 (31%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	116	1.000	Domainoid score	I1508
eggNOG	1	0.900	-	-		E1_KOG1166
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	172	1.000	Inparanoid score	I1177
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0004065
OrthoInspector	1	1.000	-	-		otm47060
orthoMCL	1	0.900	-	-		OOG6_103677
Panther	1	1.100	-	-	LDO	PTHR14030
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2819
TreeFam	0	0.000	Not matched by this tool.
	9	8.950