DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Bub1 and Bub1

DIOPT Version :9

Sequence 1:	NP_001285621.1	Gene:	Bub1 / 33758	FlyBaseID:	FBgn0031696	Length:	1099	Species:	Drosophila melanogaster
Sequence 2:	NP_001106650.1	Gene:	Bub1 / 12235	MGIID:	1100510	Length:	1059	Species:	Mus musculus

Alignment Length:	1186	Identity:	282/1186 - (23%)
Similarity:	466/1186 - (39%)	Gaps:	261/1186 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    33 MHGFLNDKQAWEHAISLYQGPDPLDHWYNYICWYENHAQSDPELKYRET-LERCLTVYEHNDYYR 96
            |....|..:.:|..:..|.|.|||..|.::|.|.|.:...:.|  |..| ||..:..:.|...|.
Mouse     1 MDNLENVFRMFEAHMQSYTGNDPLGEWESFIKWVEENFPDNKE--YLMTLLEHLMKEFLHKKNYH 63

  Fly    97 QDVRLVRLWLKYIAMQTDPLHFYQVLFQRGTGRQVAAFYIGWAAYYESREEYKDAEAVFNLAFQE 161
            .|.|.:...||:....:|...|::.|:.:|.|.:.:..|:.||.:.|::.|.:.|.|:|......
Mouse    64 NDSRFINYCLKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHN 128

  Fly   162 KAQSTSELQHAHTKFAYARSLFYQRQQQQQQQQQQHQQHPPQDALQQ--LTNYAQQQ----MPQS 220
            :|:....||..:..| .||........|....:..|.    ...|.|  :||.:.::    :|:|
Mouse   129 EAEPKELLQQQYRLF-QARLTGIHLPAQATTSEPLHS----AQILNQVMMTNSSPEKNSACVPRS 188

  Fly   221 YNQH------RPQPYQQNVYQQYHPQAQAHQAPQPHQPAPQQQLPPEQQVPY------------- 266
            ....      .....:.|:.:|........:.......||:   |..|||.|
Mouse   189 QGSECSGVASSTCDEKSNIREQRVIMISKSECSVSSSVAPK---PEAQQVMYCKEKLIRGDSEFS 250

  Fly   267 -----QTHYQERPRYEPHPATQSPTAIPPSQVQQQSHYAPVAESHYAPAQQSQLPPQQTTVPQLH 326
                 ...|.:|.::|             ..|.:..:|....|   |.|.:.||..|:  :.:||
Mouse   251 FEELRAQKYNQRKKHE-------------QWVSEDRNYMKRKE---ANAFEEQLLKQK--MDELH 297

  Fly   327 AQQPQQQQNGNGNPPPQQSPPVTN------------EVAGLRLPRNFHAYGRNNHETWKPALTLE 379
            .:..|..:..:.:.|..::.|..:            |:.|..|....|....::.|  ||.   |
Mouse   298 KKLHQVVELSHKDLPASENRPDVSLVCVGQNTCSQQELRGPSLSSISHQTSESSGE--KPQ---E 357

  Fly   380 EPDDPSRVCHYAKQLVYP----PGAGVEYSPEEILARKFKQLMDQKAKPSEPPEQEQQTLYDSYE 440
            ||..|..|......|::|    |...|..|.:.:...:.......:..|...||.::..     |
Mouse   358 EPSVPLMVNAVNSTLLFPAANLPALPVPVSGQSLTDSRCVNQSVHEFMPQCGPETKEVC-----E 417

  Fly   441 TEKSYYMTAVDGALYGQNTSSGQ-ENTGEEDEDNDAEEGEEE--------EDGGEENEEDDSDEE 496
            |.|   :.:::......|||.|. :.|..:.:.:.....:|.        :.....:..||.||.
Mouse   418 TNK---VASINDFHTTPNTSLGMVQGTPCKVQPSPTVHTKEALGFIMDMFQAPTLPDISDDKDEW 479

  Fly   497 EEDDEEEEEHSGPYTNGVQFSAQTTFEQENRSIKIKFRKEPSSTYSAYTIENVYQQQQQQQQEQH 561
            ...|:.|:          .|.||             |:|...|: ..:.::.:.
Mouse   480 PSLDQNED----------AFEAQ-------------FQKNAVSS-GDWGVKKIM----------- 509

  Fly   562 QIIHQPPQAVHHPSPDPAPASPIPIQRQRNGSHHHFHPYMLGQTSTPKSEANGYRRARTKVKR-- 624
                             ..:|..||....|..:     |.|.|   ||::..|   |||..:|
Mouse   510 -----------------TLSSAFPIFEDGNKEN-----YGLPQ---PKNKPLG---ARTFGERSL 546

  Fly   625 SKFQPDLCSNSNSA---------SSVADVASSSVLAGAPGTFNDNANFSFSSATAL--------- 671
            ||:.    |.||..         |:|..:..:..||.:|.:..|     |:||..|
Mouse   547 SKYS----SRSNEMPHTDEFMDDSTVCGIRCNKTLAPSPKSIGD-----FTSAAQLSSTPFHKFP 602

  Fly   672 --------DNSN------SSLALAVDRLNFRDTSQQQILHPVNKTLQIHNNNNNTSNNNNGTSTM 722
                    |..|      :.:||...:.|..|.|:.:.|.|:.:.:         |.:....|..
Mouse   603 ADLVQIPEDKENVVATQYTHMALDSCKENIVDLSKGRKLGPIQEKI---------SASLPCPSQP 658

  Fly   723 ADFSTFQENSYF------------ATQHD-TEAQERRLSKAVETI----ARHMDKEAIDPFNSEL 770
            |....|.:.:.|            ||..| ::|......:.|:|:    |.....|  :|::.||
Mouse   659 ATGGLFTQEAVFGLEAFKCTGIDHATVEDLSDANAGLQVECVQTLGNVNAPSFTVE--NPWDDEL 721

  Fly   771 CRAFLAKLDFPGNHDAHASYKIV---QTPLPKISNTRTLNVLEGVTFSIDKEVGRGSYGSVYKAT 832
            ....|:.|..|     ..||...   |:.||.| .|:|...|..:...::..:|.|::..|::|.
Mouse   722 ILKLLSGLSKP-----VTSYSNTFEWQSKLPAI-KTKTEYQLGSLLVYVNHLLGEGAFAQVFEAI 780

  Fly   833 DSRTGNVVA-----LKYQKPPNTWEIYICDQVLKRIKEPEVLPGVMDISTAIIAPNASLIATEFS 892
            .....|..:     ||.|:|.|:||.||..|:::|:| |||....:...:|.:..|.|::..|..
Mouse   781 HGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLK-PEVHHMFIKFYSAHLFKNGSILVGELY 844

  Fly   893 PFGSLLDINNKIRQATTKVMHESLVMHFSAQICNIVDHLHRQHIIHADIKPDNFLLMR--VPNVD 955
            .:|:||::.|..:..:.|||.::||:.|:.::..:|:.:|...|||.|||||||:|..  :...|
Mouse   845 SYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVHSCEIIHGDIKPDNFILGHRFLEQAD 909

  Fly   956 SPLPS-LRLIDFGCAIDMTLFPDGEKTKFRKVVQTDGFTCIEMQEGRSWSYETDLFCIAATVHVM 1019
            ..|.: |.|||.|.:|||.|||.|  |.|....:|.||.|.||...:.|:|:.|.|.:|||::.|
Mouse   910 EDLATGLALIDLGQSIDMKLFPKG--TVFTGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCM 972

  Fly  1020 LFGDYMQPQKKGSSWE---IRQKLPRYLKKHVWTKFFGDLLNM-QADKLPALHEMRLIFEEEAYR 1080
            |||.||:.:.:|..|:   :.::||..   .:|.:||..:||: ....||:|..:|   :.....
Mouse   973 LFGSYMKVKNEGGVWKPEGLFRRLPHL---DMWEEFFHIMLNIPDCHNLPSLDFLR---QNMKKL 1031

  Fly  1081 MDSELQKQIRTLSNIL 1096
            ::.:...:|:||.|.|
Mouse  1032 LEQQYSNKIKTLRNRL 1047

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Bub1	NP_001285621.1	Mad3_BUB1_I	38..158	CDD:214817	35/120 (29%)
Bub1	NP_001285621.1	STKc_Bub1_BubR1	813..1096	CDD:270883	99/294 (34%)
Bub1	NP_001106650.1	Necessary for kinetochore localization. /evidence=ECO:0000250	1..146		40/145 (28%)
		Mad3_BUB1_I	6..125	CDD:285506	35/119 (29%)
		Nuclear localization signal. /evidence=ECO:0000255	58..65		2/6 (33%)
		Necessary for interaction with KNL1. /evidence=ECO:0000250	99..132		8/32 (25%)
		Necessary for interaction with BUB3. /evidence=ECO:0000250	230..256		7/43 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	335..360		8/24 (33%)
		Essential for loading of BUBR1, MAD1L1 and MAD2L1 to kinetochores. /evidence=ECO:0000250	447..465		5/25 (20%)
		KEN box 1. /evidence=ECO:0000250	523..525		0/1 (0%)
		KEN box 2. /evidence=ECO:0000250	612..614		0/1 (0%)
		PKc_like	761..1047	CDD:304357	102/300 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167832000
Domainoid	1	1.000	143	1.000	Domainoid score	I4633
eggNOG	1	0.900	-	-		E1_KOG1166
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1411806at2759
OrthoFinder	1	1.000	-	-		FOG0004065
OrthoInspector	1	1.000	-	-		otm42662
orthoMCL	1	0.900	-	-		OOG6_103677
Panther	1	1.100	-	-	LDO	PTHR14030
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2819
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	12	11.800