DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col4a6

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_444415.2	Gene:	Col4a6 / 94216	MGIID:	2152695	Length:	1691	Species:	Mus musculus

Alignment Length:	1826	Identity:	786/1826 - (43%)
Similarity:	939/1826 - (51%)	Gaps:	285/1826 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 KNCTAGYAGC--VPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDK 133
            |.|  |...|  |..|..|||.||.|||:|..|..|..||.|..|.||.||::|.|||.|..|.|
Mouse    29 KPC--GSRDCHGVCTCFPEKGARGHPGPIGTQGPAGPEGFTGPTGLSGLKGERGSPGPLGPYGSK 91

  Fly   134 GERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGE 198
            |::|..|:.|..|:.|:.|..|.||..|..|.|||:|..|..|..|..|.||..|..|..|.||.
Mouse    92 GDKGPIGVPGFVGISGIPGHPGQPGPRGPPGPDGCNGTQGAVGFPGPDGYPGILGPPGLPGHKGA 156

  Fly   199 KGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASC 263
            ||||....|... |.:|:||..|..|:.||.|..|..|:.|..||.|.:||.|..||.|.:|...
Mouse   157 KGEPVPFQGSIT-GIRGDPGLPGLDGIPGPSGSSGSPGDAGPIGPPGLQGPPGLPGLPGPEGNMG 220

  Fly   264 YGPMKPGAPGIKGEKGEPASSFPVKPTHTV--MG-PRGDMGQKGEPGLVGRKGEPGPEG--DTGL 323
            .|  ..|..|:||:.|.|..:.|...|..:  || |:|..|.|||||..|..|..||.|  :.|.
Mouse   221 LG--FQGEKGVKGDVGLPGPAGPPPSTGELEFMGFPKGKKGSKGEPGPPGFPGMSGPPGVPEFGS 283

  Fly   324 DGQKGEKGLPGGPGDRGRQGN---FGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPG--------- 376
            .|:|||||:.|.||.||..|:   .|.||..|:||..|.||:||.||..|:||:.|
Mouse   284 TGEKGEKGILGLPGPRGPMGSEGIQGHPGRQGKKGTSGFPGINGFPGIKGEKGDIGVRGPDSFTD 348

  Fly   377 -----RAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQK 436
                 .:|..|.||:||.||.    ||..|..|.:||.   |.||...|.|:.|..||||:.|.|
Mouse   349 ADGAVISGYPGDPGVLGLPGL----RGDEGVQGQRGPS---GTPGLPSLTGLPGALGPQGFPGLK 406

  Fly   437 GGAGLPGRP--GNEGPPGKKGEKGTAGLNGPKGSIG---PIGH-----PGPPGPEGQKGDAGLPG 491
            |..|..||.  |..|.||:.|..|..||.||.|..|   ..||     ||.||.:|::|..|  .
Mouse   407 GDQGNSGRTTFGEAGLPGRVGLPGLPGLPGPSGPPGRTFETGHLSNIEPGFPGLQGEQGPKG--H 469

  Fly   492 YGIQGSKGDAGI---------------PGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGA 541
            .|::|.|||:|.               ||.||::|..|.:||||..|.||..     |..|.||.
Mouse   470 QGLKGVKGDSGFCACEGGAPNIGPHGEPGLPGIQGPIGLQGFKGTKGDPGSR-----GASGPAGT 529

  Fly   542 PGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPP------- 599
            ||..|..|:.|..|:||:..:..  |.|.|..:..| |.:||.||.|.|||||..|.|
Mouse   530 PGLFGPRGQTGLKGKKGEPTVSR--GSKMSGDKGDP-GPQGTPGLAGTPGKDGRPGLPGLPGIQG 591

  Fly   600 -GERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPG 663
             |..|:|||||..|:      |||||.||..|.||.                      |.||.||
Mouse   592 DGGSGFPGERGLPGL------PGEKGHDGPIGPPGI----------------------GLPGPPG 628

  Fly   664 AKGVQGFKGAEGLPGIPGPKGEFG------FKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKG 722
            .:|:.|.||.:||||..|.:|..|      ..|..|.||.||..|.||..|..|.||||     |
Mouse   629 PRGLPGDKGVDGLPGQQGLRGAQGVTLPCIIPGSYGPSGFPGAPGFPGSKGARGLPGIP-----G 688

  Fly   723 EPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGY 787
            :||.||..|..|..|.....|..|.||          .:|.||.|   |.||.||:||.||:.|.
Mouse   689 KPGTHGSKGGPGSPGLMHLPGLPGFPG----------VRGEKGLP---GFPGLPGKDGYPGKAGS 740

  Fly   788 TGLKGNTGPQGPP-GVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQP---GPRGEP 848
            .||.|..|..|.. |.|     ||..||:|.||..|..|.||..||.|..|.||:|   ||:||.
Mouse   741 PGLPGFKGAAGDIFGAE-----NGASGEQGLQGLPGDKGFPGDSGLPGPKGLNGKPGMLGPKGER 800

  Fly   849 GISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDV 913
            |  .||..|||      |:.|..|.|.|.|..|..|..|.|      ||||:||.||..|::||:
Mouse   801 G--NPGTSGPP------GQPGPSGSTDPFGIKGTSGFPGAP------GLPGISGHPGKKGQRGDI 851

  Fly   914 GPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGK 978
            |..|..|..|.||:.|:.|.:|..|..|.||..|:.|:||.:|.:|..|:.||.||.|:.|.||.
Mouse   852 GQPGSTGKRGLPGIKGLPGPQGSPGFLGSPGLSGVTGLPGIEGQKGEKGSSGPVGFPGLPGLPGL 916

  Fly   979 RGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGL---------MGIKGDQGLAGAPGQQG 1034
            .|..|:.|.||:.|..|..|.||..|..|.||.||..|:         :..||::|..|:.|..|
Mouse   917 PGADGLKGFSGSFGKVGQPGQTGTSGEKGDRGDPGPVGISSPRPPMLNLWFKGEKGSRGSAGSDG 981

  Fly  1035 LDGMPGEKGNQGFPGLDGPPGLPGDASE-KGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPG---- 1094
            ..|..|:||..|.|||.||||..|..:. ||..|.|            |:||..|.:||||
Mouse   982 FPGPRGDKGEPGIPGLPGPPGATGQTNTIKGLNGRP------------GSPGSMGRRGLPGLKGS 1034

  Fly  1095 LAVHGRAGPPGEKGDQ---GRSGIDGRDGINGEKGEQG-LQGVWGQPGEKGSVGAPGIPGAPGMD 1155
            |.:.|..|.||:.|.|   |.||:.|..||.|.||:|| ..|:.|.||.||..|..|..|..|.|
Mouse  1035 LGIAGFPGMPGKSGSQGLTGTSGLLGATGIPGLKGDQGQTLGISGSPGPKGQPGELGFKGVKGKD 1099

  Fly  1156 GLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPAT 1220
            ||.|..      ||||::||.|:.|::|.|            ||.|:||.:|..|:.|.||||
Mouse  1100 GLVGDR------GYPGNKGDGGKVGITGDP------------GFPGSPGLQGISGMNGDPGLP-- 1144

  Fly  1221 VPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASG--------LNGIPG 1277
                       |..|:.|..|..|..||.||.|..||.|..||.|.||..|        :.|:||
Mouse  1145 -----------GSSGHLGSIGRPGPSGLIGPKGFPGAPGLHGLNGLPGTKGTHGTPGASITGVPG 1198

  Fly  1278 AKGDIGPRGEIGYPGVTI--------KGEKGLPGRPGRNGRQGLIGAPGLI---------GERGL 1325
            ..|..||:||.|.||:.|        ||:||..|.||..|..|..||||:.         |:.|.
Mouse  1199 PAGLPGPKGERGVPGIVIGDPGKQGSKGQKGDQGSPGLQGPAGTPGAPGISLPSVIAGQPGDPGR 1263

  Fly  1326 PGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEG 1390
            |||.||.|..|.|||.||.|...::      |.||..||||.|||      |||||.:||.||.|
Mouse  1264 PGLDGERGRPGPPGPPGPPGPSSDQ------GDPGDSGFPGIPGL------QGFKGNQGLPGFSG 1316

  Fly  1391 QKGDKGDRGLQGPSGL---PGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLP 1452
            ..|:.|.:|::|..||   ||.:||.||.|:||:.|..||      |||:||:|..|.  ||
Mouse  1317 LSGELGLKGMRGEPGLMGTPGKIGQPGDPGFPGMKGKAGP------RGFSGPQGAPGH--TP--- 1370

  Fly  1453 GQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGP 1517
               ....|..||||.|.||..|..|..|:||..|..||      :|.||..|:.|:.|..|.|||
Mouse  1371 ---IAEAMQVPPGPLGLPGIDGIPGLIGDPGSQGSVGL------QGSKGLPGIPGKDGPSGLPGP 1426

  Fly  1518 KGDRGEP------GERGYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTE 1576
            .|..|:|      |..|:|||.|..|..|:||.|....   ..|..:.:|||||.||.|..|.:.
Mouse  1427 SGILGDPGLPGLQGPPGFEGAPGNQGPIGQPGMPGHGV---RVGYTLVKHSQSEQVPLCPTGMSR 1488

  Fly  1577 LWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPM 1641
            ||.|||||:|:|.:.|||||||..|||:|||||:|.:.|..|.||:||.||||::||:|.|.|||
Mouse  1489 LWVGYSLLFVEGQEKAHNQDLGFAGSCLPRFSTMPFIYCNINEVCHYARRNDKSYWLSTTAPIPM 1553

  Fly  1642 MPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQAL 1706
            |||...:|.||||||.|||||:..||||||.|.||.||.||..||||||||||||.|..||||:|
Mouse  1554 MPVGQTQIPQYISRCSVCEAPSQAIAVHSQDIIVPQCPLGWHSLWIGYSFLMHTAAGAEGGGQSL 1618

  Fly  1707 QSPGSCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPF-ERPQQQTIKAGERQSHVSR 1770
            .|||||||||||||||||:||:||||::....|||:..:|....| |.|..:|:|.|:..:.|||
Mouse  1619 VSPGSCLEDFRATPFIECSGARGTCHYFANKYSFWLTTVEERGQFREEPVSETLKTGQLHTRVSR 1683

  Fly  1771 CQVCMK 1776
            ||||||
Mouse  1684 CQVCMK 1689

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	26/56 (46%)
		Collagen	322..380	CDD:189968	30/74 (41%)
		Collagen	413..465	CDD:189968	24/53 (45%)
		Collagen	499..561	CDD:189968	28/76 (37%)
		Collagen	574..632	CDD:189968	30/65 (46%)
		Collagen	657..714	CDD:189968	27/62 (44%)
		Collagen	765..824	CDD:189968	27/59 (46%)
		Collagen	854..911	CDD:189968	23/56 (41%)
		Collagen	884..943	CDD:189968	25/58 (43%)
		Collagen	923..982	CDD:189968	26/58 (45%)
		Collagen	1028..1085	CDD:189968	21/57 (37%)
		Collagen	1229..1287	CDD:189968	25/65 (38%)
		Collagen	1318..1376	CDD:189968	26/66 (39%)
		Collagen	1399..1458	CDD:189968	25/61 (41%)
		Collagen	1477..1534	CDD:189968	25/62 (40%)
		C4	1555..1662	CDD:128421	65/106 (61%)
		C4	1663..1777	CDD:128421	73/115 (63%)
Col4a6	NP_444415.2	Collagen	492..548	CDD:189968	25/60 (42%)
		Collagen	664..738	CDD:189968	39/91 (43%)
		Collagen	843..900	CDD:189968	23/56 (41%)
		Collagen	1118..1175	CDD:189968	28/81 (35%)
		Collagen	1328..1397	CDD:189968	35/82 (43%)
		Collagen	1401..1460	CDD:189968	26/64 (41%)
		C4	1468..1572	CDD:279721	62/103 (60%)
		C4	1576..1688	CDD:279721	70/111 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	146	1.000	Domainoid score	I4520
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1346	1.000	Inparanoid score	I143
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	1	1.000	-	-		mtm8822
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	10.870