DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col3a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_114474.1	Gene:	Col3a1 / 84032	RGDID:	71029	Length:	1463	Species:	Rattus norvegicus

Alignment Length:	1688	Identity:	620/1688 - (36%)
Similarity:	725/1688 - (42%)	Gaps:	453/1688 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    57 IVDSAGVARGDL----PPKNCTAGYAGCVP--KCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPS 115
            :.||..|...|:    .|.:|....   :|  :|.|.......|.|:.|.|             :
  Rat    55 VCDSGSVLCDDIMCDDEPLDCPNPE---IPFGECCAICPQPSTPAPVIPDG-------------N 103

  Fly   116 GDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPG------APGINGKDGCDGQDGI 174
            ..:|.||||||   .|..|..|.|||.||   ||:.||.|:||      ..|.|.....|..|..
  Rat   104 RPQGPKGDPGP---PGIPGRNGDPGLPGQ---PGLPGPPGSPGICESCPTGGQNYSPQFDSYDVK 162

  Fly   175 PGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERG 239
            .|:.|:.|.|||.|..|..|..|..|.|         |..|.||::      ||.|.||:.|..|
  Rat   163 SGVGGMGGYPGPAGPPGPPGPPGSSGHP---------GSPGSPGYQ------GPPGEPGQAGPAG 212

  Fly   240 DSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKG 304
            ..||.||.||.|..|..||.|       :||.|   ||:|.|             ||.|..|..|
  Rat   213 PPGPPGAIGPSGPAGKDGESG-------RPGRP---GERGLP-------------GPPGIKGPAG 254

  Fly   305 EPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNP 369
            .||.      ||.:|..|.||:.||||..|.||.:|..|..|..|:.|..|.||.||..|.||.|
  Rat   255 IPGF------PGMKGHRGFDGRNGEKGETGAPGLKGENGLPGDNGAPGPMGPRGAPGERGRPGLP 313

  Fly   370 GQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNG 434
            |..|..|..||.|..|..|||||||.. |.||.||.||..|..|:||..|..|..|.|||||:.|
  Rat   314 GAAGARGNDGARGSDGQPGPPGPPGTA-GFPGSPGAKGEVGPAGSPGSNGSPGQRGEPGPQGHAG 377

  Fly   435 QKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKG 499
            .:|.   ||.|||.|.||.|||            :||
  Rat   378 AQGP---PGPPGNNGSPGGKGE------------MGP---------------------------- 399

  Fly   500 DAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKG 564
             |||||.|||.|::|.                 ||..||.|||||:|.:|.
  Rat   400 -AGIPGAPGLLGARGP-----------------PGPAGANGAPGQRGPSGE-------------- 432

  Fly   565 DVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRT 629
                                     |||:||:|.||.||..||.|..||      ||.|||||:.
  Rat   433 -------------------------PGKNGAKGEPGARGERGEAGSPGI------PGPKGEDGKD 466

  Fly   630 GLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGL 694
            |.|   ||||.               .|.||.||.:|..||:|..|..|.||.||..|.:|..|.
  Rat   467 GSP---GEPGA---------------NGVPGNPGERGAPGFRGPAGPNGAPGEKGPAGERGGPGP 513

  Fly   695 SGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMP 759
            :|..|..|.|||.|..|.|||.|  :.|.||..|.||..|..||.|.||..|.||
  Rat   514 AGPRGVAGEPGRDGTPGGPGIRG--MPGSPGGPGNDGKPGPPGSQGESGRPGPPG---------- 566

  Fly   760 AKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVP 824
            ..|.:|:||..|.|||.|.||:||:.|..|     || |.||:.||.|.||..|.:|..|..|.|
  Rat   567 PSGPRGQPGVMGFPGPKGNDGAPGKNGERG-----GP-GGPGLPGPAGKNGETGPQGPPGPTGAP 625

  Fly   825 GNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYP 889
            |:.|.         .|.|||:|..||  ||..||||.||..||.|.:|..|..|.||..|..|.|
  Rat   626 GDKGD---------AGPPGPQGLQGI--PGTSGPPGENGKPGEPGPKGEAGAPGVPGGKGDSGAP 679

  Fly   890 GDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGN 954
            |:||.   ||.:|.||:.|..|..||.|..|.|||||.||..            |.|||.|||
  Rat   680 GERGP---PGTAGTPGLRGGAGPPGPEGGKGPAGPPGPPGTS------------GPPGLQGMP-- 727

  Fly   955 KGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMG 1019
             |:||.||:.|||   |..|.||..|..|:|               |.|||.|..||.|.||..|
  Rat   728 -GERGGPGSPGPK---GEKGEPGGAGADGVP---------------GKDGPRGPAGPIGPPGPAG 773

  Fly  1020 IKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTP 1084
            ..||:|..|||               |.||:.||.|.||   |:|:.|.|         ||||.|
  Rat   774 QPGDKGEGGAP---------------GLPGIAGPRGGPG---ERGEHGPP---------GPAGFP 811

  Fly  1085 GWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIP 1149
            |.||:.|.||  ..|..|.|||||:.|..|..|..|.:|..|..|.|||   .||:||.|.||..
  Rat   812 GAPGQNGEPG--AKGERGAPGEKGEGGPPGAAGPPGGSGPAGPPGPQGV---KGERGSPGGPGAA 871

  Fly  1150 GAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQ 1214
            |.||..||||.         ||:.|:.|.||.||.|   |:.||         |||.|..|..|.
  Rat   872 GFPGGRGLPGP---------PGNNGNPGPPGPSGAP---GKDGP---------PGPAGNSGSPGN 915

  Fly  1215 PGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAK 1279
            ||       :.|.||..|:.|..|..|.||..|..||.|:||..|.|||.|||      |:||.:
  Rat   916 PG-------VAGPKGDAGQPGEKGPPGAQGPPGSPGPLGIAGLTGARGLAGPP------GMPGPR 967

  Fly  1280 GDIGPRGEIGYPGVTIKGEKGLPGRPGRNGR------QGLIGAPGLIGERGLPGLAGEPGLVGLP 1338
            |..||:|        ||||.|.||..|.||.      |||.|.||..||   ||..|.||..|.|
  Rat   968 GSPGPQG--------IKGESGKPGASGHNGERGPPGPQGLPGQPGTAGE---PGRDGNPGSDGQP 1021

  Fly  1339 GPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGP 1403
            |..|..|.||:||..||||.||..|.||.|   |..||            .|:.||:|:.|..||
  Rat  1022 GRDGSPGGKGDRGENGSPGAPGAPGHPGPP---GPVGP------------SGKNGDRGETGPAGP 1071

  Fly  1404 SGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKG 1468
            ||.||..|.:               ||||.:|..|.||..|..|:.|:.|.:|.||   .|||.|
  Rat  1072 SGAPGPAGAR---------------GAPGPQGPRGDKGETGERGSNGIKGHRGFPG---NPGPPG 1118

  Fly  1469 EPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAI 1533
            .||..|..|..|.||..|.||.:|..|..|:.|..   |..|.:|.|||:|:|   ||||.||:.
  Rat  1119 SPGAAGHQGAVGSPGPAGPRGPVGPHGPPGKDGSS---GHPGPIGPPGPRGNR---GERGSEGSP 1177

  Fly  1534 GLIGQKGEPGAP-APAALDYLTGILITRHSQSETVPACSAGHT------ELWTGYSLLYVDG-ND 1590
            |..||.|.||.| ||.                   |.|..|..      |...|:|..|.|. .|
  Rat  1178 GHPGQPGPPGPPGAPG-------------------PCCGGGAAIAGVGGEKSGGFSPYYGDDPMD 1223

  Fly  1591 YAHNQD--LGSPGSCVPRFSTL---------PVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPV 1644
            :..|.:  :.|..|...:..:|         |..:|.....|:...::.: :|:..|....|..:
  Rat  1224 FKINTEEIMSSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGE-YWVDPNQGCKMDAI 1287

  Fly  1645 ENIEIRQYISRCVVCEAPANVIAVHSQTIEVP------DCPNGWEGLWIGYSFLMHTAVGNGG 1701
            :           |.|........:::..:.||      |.....:.:|.|.|.       |||
  Rat  1288 K-----------VFCNMETGETCINASPMTVPRKHWWTDAGAEKKHVWFGESM-------NGG 1332

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	22/62 (35%)
		Collagen	322..380	CDD:189968	27/57 (47%)
		Collagen	413..465	CDD:189968	24/51 (47%)
		Collagen	499..561	CDD:189968	21/61 (34%)
		Collagen	574..632	CDD:189968	23/57 (40%)
		Collagen	657..714	CDD:189968	26/56 (46%)
		Collagen	765..824	CDD:189968	28/58 (48%)
		Collagen	854..911	CDD:189968	27/56 (48%)
		Collagen	884..943	CDD:189968	24/58 (41%)
		Collagen	923..982	CDD:189968	26/58 (45%)
		Collagen	1028..1085	CDD:189968	19/56 (34%)
		Collagen	1229..1287	CDD:189968	26/57 (46%)
		Collagen	1318..1376	CDD:189968	28/57 (49%)
		Collagen	1399..1458	CDD:189968	21/58 (36%)
		Collagen	1477..1534	CDD:189968	25/56 (45%)
		C4	1555..1662	CDD:128421	21/124 (17%)
		C4	1663..1777	CDD:128421	9/45 (20%)
Col3a1	NP_114474.1	VWC	33..89	CDD:278520	9/36 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	97..1195		580/1485 (39%)
		Nonhelical region (N-terminal)	155..169		4/13 (31%)
		Triple-helical region	170..1195		549/1393 (39%)
		Collagen	233..292	CDD:189968	31/87 (36%)
		Collagen	278..331	CDD:189968	24/52 (46%)
		Collagen	353..424	CDD:189968	42/131 (32%)
		Collagen	413..472	CDD:189968	37/123 (30%)
		Collagen	452..505	CDD:189968	31/76 (41%)
		Collagen	629..686	CDD:189968	30/70 (43%)
		Collagen	<1061..1103	CDD:189968	21/56 (38%)
		COLFI	1230..1462	CDD:279718	20/122 (16%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810