DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and si:ch211-196i2.1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_009293536.1	Gene:	si:ch211-196i2.1 / 564099	ZFINID:	ZDB-GENE-091204-150	Length:	1813	Species:	Danio rerio

Alignment Length:	1714	Identity:	588/1714 - (34%)
Similarity:	720/1714 - (42%)	Gaps:	457/1714 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    28 TAGTAGSIQDSVKHYNRN-----------EPKFPIDDSYDIVDSAGVARGDLPPKNCTAGYAGCV 81
            |:...|.::.:.||.:.|           .||.|:|.:...:|.:....|...|...........
Zfish   337 TSSRDGKLESTSKHLDENITIDKHKPGKPLPKKPVDTTIINLDVSEKPTGSTVPSREIHLMTTSS 401

  Fly    82 PKCIAEKGNRGL-----------PGPL---------GPTGLKGEMGFPGMEGPSGDKGQKGDPGP 126
            |    |.|...|           ||..         |.|..|.    |.:...||. |.:..|..
Zfish   402 P----EDGESNLLKEDFEVTTQAPGRTSWDNVSKGSGRTPSKS----PDIHAGSGG-GSEEHPDT 457

  Fly   127 YGQRGDKGE--RGSPG--LHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPR 187
            ....|.:|:  |||.|  ...|.|..|..||.|.||.|                  |.:|.|||:
Zfish   458 IVLTGRQGDIVRGSDGKMYRIQRGPMGPMGPPGEPGCP------------------GNAGYPGPK 504

  Fly   188 GYAGQLGSKGEKGEPAKENGDYAKGEKGEPG------WRGT-----------------AGLAGPQ 229
            |..|..|.:|..|.|.      ..|..|.||      ||.|                 ||....|
Zfish   505 GDKGAFGVRGRPGRPG------FLGSPGPPGLPSFYLWRNTPEDWAAFQQTSFFQLLLAGWPRAQ 563

  Fly   230 GFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVM 294
            |.||.:||.|..   ||:||                   ||.|   ||:|.|
Zfish   564 GLPGPEGEMGKP---GAQGP-------------------PGEP---GERGPP------------- 590

  Fly   295 GPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGE 359
            |..||||.:|..|:|||.|..|.:|:.||||.:|..|:|      |.:|..|..|.:|..|::||
Zfish   591 GRMGDMGDRGPKGVVGRAGVYGRDGENGLDGPQGPSGIP------GPKGPLGYKGESGSHGEKGE 649

  Fly   360 PGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVD 424
            .|:.|..|..|.||:||..|:                :|.||.||..||      |||||:.|::
Zfish   650 EGITGSEGPCGDKGKPGEKGS----------------KGAPGAPGAVGP------PGPQGIRGME 692

  Fly   425 GL---PGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGD 486
            ||   |||.|.:|..|..||||.||..|..|..|.:|..|..|..|..|.:|.|||.||:|.:|.
Zfish   693 GLEGHPGPDGEDGMNGSPGLPGAPGAPGWTGLIGAQGANGSRGEPGPSGAVGLPGPQGPQGLEGQ 757

  Fly   487 AGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRP 551
            .|.|     |.:|..|:.|..||.|.|||.|..|..|..||              ||.:|..|..
Zfish   758 IGPP-----GQRGPPGLSGREGLHGPKGEPGPVGPVGIRGD--------------PGFEGLKGVL 803

  Fly   552 GTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQ 616
            ||||.||..||||:.|........||||:||..|..|:.|..|.:||.|..|:|      ||.|.
Zfish   804 GTPGPKGFTGIKGNRGPDGDQGDIGPKGNKGARGAAGVQGIQGEQGPIGFPGFP------GIRGP 862

  Fly   617 TGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPG 681
            .||.|.:||||..|..|..|:                           :|..|.:|.|||.|.||
Zfish   863 PGPQGNQGEDGEAGPHGKVGK---------------------------EGSTGSRGPEGLSGKPG 900

  Fly   682 PKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKG 746
            |:|..|..|::|.:|.||.            ||:||.  .|..|..|:.|.:|.:|..|.:|.||
Zfish   901 PRGLKGRTGQRGHTGMPGP------------PGLPGP--PGPFGAEGKPGTQGLQGLVGNAGNKG 951

  Fly   747 EPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGP 811
                         :.|.:|.||:.|..||||..|.||..         ||.|.||..|.||..|.
Zfish   952 -------------STGLQGIPGERGQDGPPGTMGPPGRE---------GPMGSPGPPGERGFGGK 994

  Fly   812 RGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGP 876
            .|.||.||.||:.|.||..|:||.||..|.   ||||                    |.||||||
Zfish   995 TGMKGPQGPVGMYGFPGTVGMRGRPGHTGD---RGEP--------------------GPRGPTGP 1036

  Fly   877 IGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKG 941
            ||..|..|.   ||..|:.||||..||.|..|.:|..||.||.|..|..||.|..|.||..|..|
Zfish  1037 IGKTGVPGP---PGPVGEKGLPGPQGRQGDPGHQGTDGPCGPPGRHGVSGVIGKPGPRGSIGPPG 1098

  Fly   942 EPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPV 1006
            |||..|..|:||.:|:.|.||.||.||..|:.|:||..||.|:.|..|..|..|..|..|..|..
Zfish  1099 EPGPSGPPGIPGPQGENGVPGIDGEKGEKGMMGSPGDDGPLGLEGQQGLTGPAGLDGAPGRKGDK 1163

  Fly  1007 GGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGP 1071
            |.|||.|..||.|.|||   .|..|.:|..|.||..||:|.   .||.|:.||...||:||:.||
Zfish  1164 GDRGPIGTSGLQGPKGD---IGPVGDKGPPGPPGLVGNEGD---SGPAGVAGDPGSKGEKGDIGP 1222

  Fly  1072 SGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQ 1136
            .|..|..||     |                  |:.|:||..||.|..|..|.:||.||.|..|.
Zfish  1223 PGFTGLEGP-----W------------------GDTGEQGEKGIKGAKGQIGMQGEPGLVGGVGH 1264

  Fly  1137 PGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTG 1201
            ||.||:.|.||.||..|.||..|:.|..|..|.||:.|:.|..|::|..|::|:||..|.||..|
Zfish  1265 PGLKGAEGVPGHPGLVGPDGPAGSKGKTGPSGSPGEPGNPGPEGIAGPKGVRGDTGKGGTQGGVG 1329

  Fly  1202 APGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGP 1266
            ..||.|.                .|.||..|..|..||.|::||.||.||.|:      :|:|||
Zfish  1330 LVGPIGP----------------LGPKGLSGPEGEKGEPGDKGEPGLEGPDGL------QGVQGP 1372

  Fly  1267 PGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGE 1331
            ||..||.|                    .:||.|:.|.||..|.||.:|.||..|..|:.|..|:
Zfish  1373 PGLPGLEG--------------------SQGEAGMRGPPGLPGAQGGVGKPGPDGREGMKGEKGD 1417

  Fly  1332 PGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKG 1396
            .|..|.||.:|..|.:|:||.|               ||:...||:|.|||         |||.|
Zfish  1418 QGKNGAPGKLGHIGRRGKRGKA---------------GLRATRGPRGEKGE---------KGDVG 1458

  Fly  1397 DRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML 1461
            ::||.|..|..|:.|.:|:.      |::|..||.||:|..||.|..||||..|..|..|.||
Zfish  1459 EKGLPGWGGSMGIQGPRGEP------GDEGVKGAEGEKGDQGPFGSPGRDGIKGSLGPLGPPG-- 1515

  Fly  1462 PPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGE 1526
             ||||||:.|..|.:||:|.||.||..||.|.:|.:|.:|.            |||.|.||.|
Zfish  1516 -PPGPKGDQGNIGPSGPRGTPGIPGLPGLFGDKGLKGFQGP------------PGPPGTRGLP-- 1565

  Fly  1527 RGYEGAIGLIGQKGEPGAPAPAALDY-LTGILITRHSQSETVPACSAGHT--------------- 1575
                         |.||.|.|..:.. ||.|.|.....|...|..|...|
Zfish  1566 -------------GPPGPPGPPGISVNLTLIQIKDLMYSSDKPNFSLIQTLLDSLYHDLQLLVDP 1617

  Fly  1576 -------------ELW-------TGYSLLYVDGND---------YAHNQDLGSPGSCVPRFSTLP 1611
                         |||       :|:  .|:|.|.         |.:..:.|:.....||.:.||
Zfish  1618 PDGTKQHPASTCLELWLCHPNYTSGF--YYIDPNQGSPLDALLAYCNFSESGAETCLHPRDAHLP 1680

  Fly  1612 VLSCGQNNVCNYASRNDKTFWLTT 1635
            .     ....|.:..|.|..||::
Zfish  1681 T-----RTWLNDSGNNSKFHWLSS 1699

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	19/60 (32%)
		Collagen	322..380	CDD:189968	23/57 (40%)
		Collagen	413..465	CDD:189968	25/54 (46%)
		Collagen	499..561	CDD:189968	24/61 (39%)
		Collagen	574..632	CDD:189968	26/57 (46%)
		Collagen	657..714	CDD:189968	17/56 (30%)
		Collagen	765..824	CDD:189968	27/58 (47%)
		Collagen	854..911	CDD:189968	22/56 (39%)
		Collagen	884..943	CDD:189968	27/58 (47%)
		Collagen	923..982	CDD:189968	29/58 (50%)
		Collagen	1028..1085	CDD:189968	24/56 (43%)
		Collagen	1229..1287	CDD:189968	22/57 (39%)
		Collagen	1318..1376	CDD:189968	17/57 (30%)
		Collagen	1399..1458	CDD:189968	24/58 (41%)
		Collagen	1477..1534	CDD:189968	20/56 (36%)
		C4	1555..1662	CDD:128421	24/125 (19%)
		C4	1663..1777	CDD:128421
si:ch211-196i2.1	XP_009293536.1	LamG	67..217	CDD:304605
		Collagen	573..631	CDD:189968	32/101 (32%)
		Collagen	618..677	CDD:189968	27/80 (34%)
		Collagen	663..720	CDD:189968	31/78 (40%)
		Collagen	699..757	CDD:189968	26/57 (46%)
		Collagen	819..877	CDD:189968	26/63 (41%)
		Collagen	855..907	CDD:189968	26/84 (31%)
		Collagen	1002..1076	CDD:189968	42/99 (42%)
		Collagen	1200..1257	CDD:189968	28/82 (34%)
		Collagen	1254..1312	CDD:189968	27/57 (47%)
		Collagen	1338..1396	CDD:189968	30/83 (36%)
		Collagen	1371..1425	CDD:189968	25/73 (34%)
		Collagen	1434..1491	CDD:189968	29/86 (34%)
		COLFI	1618..1813	CDD:295304	18/89 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910