DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col4a4

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_009290110.1	Gene:	col4a4 / 554270	ZFINID:	ZDB-GENE-101001-1	Length:	1670	Species:	Danio rerio

Alignment Length:	1884	Identity:	753/1884 - (39%)
Similarity:	928/1884 - (49%)	Gaps:	341/1884 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 FWKRLLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRNEPKFPIDDSYDIVDSAGVARGDL 68
            |.:|||.|.::....:|.|    ||....|
Zfish    14 FCRRLLLALLMIQTTLGGD----KTEECGG----------------------------------- 39

  Fly    69 PPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDK 133
              ::|:      |.:|...||.:|.||.||..|.:|..|.||..|..||||::|..|..|..|.|
Zfish    40 --RDCS------VCQCFPPKGEQGNPGMLGRQGPQGPQGSPGPPGLRGDKGERGTIGFPGVSGPK 96

  Fly   134 GERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGE 198
            ||.|:.|:.|.||:.||.|..|..|.||:.|||||:|..|..||.|..|.||..|..|..|.||.
Zfish    97 GELGARGVEGHAGLDGVPGHPGPGGLPGLPGKDGCNGTKGECGLNGEVGRPGLPGPVGLPGFKGA 161

  Fly   199 KGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASC 263
            ||||.... .|.   .|:||..|..|:.||||..|..|.:|..|..|:.||:|:.||||.:|
Zfish   162 KGEPFYRT-IYL---PGKPGADGPPGMVGPQGLQGIAGPKGPPGWVGSDGPKGKPGLKGPRG--- 219

  Fly   264 YGPMKPGAPG--IKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPG------------LVGRKGE 314
                .||.||  :.|.||:                .||.|.:|:||            :.|.|||
Zfish   220 ----PPGDPGRSLAGPKGD----------------EGDQGIQGDPGPFEYVEPNPEDYIKGEKGE 264

  Fly   315 PGPEGDTGLDGQ---------KGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPG 370
            .||.|..|:.||         |||||:.|.||.||..|.   ||.||::|.:.|.|:|||||..|
Zfish   265 QGPRGYIGIPGQEGPEGPSAEKGEKGVIGLPGVRGPPGY---PGQTGRQGPKAERGVNGLPGLYG 326

  Fly   371 QK---GEPGRAGATGKP--------GLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVD 424
            .|   |.||..|..|||        ||.|.|||||.       ||..|.||.:|.|||.|..|:
Zfish   327 TKGLMGIPGDPGPKGKPVESQKYVIGLPGDPGPPGN-------PGAPGDRGLMGIPGPSGDPGL- 383

  Fly   425 GLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAG-LNGPKGSIGPIGHPGPPGPEGQKGDAG 488
            .|||..|..|..|.:||            ||:||.|| :.|...|.|..|..|.|||:||||:.|
Zfish   384 SLPGAMGDTGLMGLSGL------------KGQKGGAGSIYGSLLSYGEPGKRGFPGPKGQKGNPG 436

  Fly   489 LPGY-------GIQGSKGDAGIPGYPGLKGSKGERGFKGNA-GAPGDSKLGRPGTPGAAGAPGQK 545
            .|||       |..|.||:.||.||.|::|.||..|  |.| ..|||    .||.||..|.||:.
Zfish   437 TPGYYCLPGERGDLGRKGERGIVGYKGVQGRKGPDG--GCACDPPGD----LPGDPGPPGPPGEP 495

  Fly   546 GDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGE--- 607
            ||.|:.|..|..|::|:.||                  :|:.||.|..|..||.|.:|..||
Zfish   496 GDPGQKGFRGPLGEIGLSGD------------------NGVNGITGSRGLTGPRGVKGKKGEVMT 542

  Fly   608 ---RGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQG 669
               :|..|..|.:|..|::|..|::|:.|..|:||.|......|..| .||:|..||.|.|||:|
Zfish   543 LSIKGEKGDLGPSGQHGKQGYTGKSGVDGIDGDPGDPGPLGDDLFGP-PGDRGCQGAIGVKGVRG 606

  Fly   670 FKGAEG--LPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGA 732
            ..|..|  :.|..|.:|:.|..|.:||:|.||..|..|.....| |||||..           |.
Zfish   607 QTGPPGGCIMGSIGRRGQQGDPGPQGLAGFPGPKGLQGETLPCG-PGIPGPR-----------GC 659

  Fly   733 KGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQ 797
            ||..|..|..|:.|.|| |.               ||.|..||.||.|.||:.|.||.:|..|..
Zfish   660 KGMPGGIGDKGKLGNPG-CI---------------GQNGTRGPKGEIGEPGDCGITGQRGPDGYL 708

  Fly   798 GPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLN 862
            |.||..||.|::       ..|:.|:||:.|.:||:         |..|:.....|||:||.|:.
Zfish   709 GDPGEPGPCGIS-------INGSPGIPGSRGPEGLK---------GSCGDRMFGAPGPLGPSGVQ 757

  Fly   863 GLQGEKGDRGPTGPIGFPGADGSVGYP---GDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGP 924
            |.||.||..||.||||..|..|..|||   |:||..|.||..||||...|:.|.|..|..|..|.
Zfish   758 GCQGPKGVPGPPGPIGLQGMSGVPGYPGRKGERGKDGAPGPPGRPGKSPEQCDKGDEGLPGKKGE 822

  Fly   925 PGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSG 989
            .|:.|..|..|..|:.||.|.||:    |.||.||.||..||:|..|:.|..|..|..|.||..|
Zfish   823 QGLIGHRGYPGEKGSLGELGCPGI----GAKGARGKPGTPGPQGCIGLQGPAGVNGDGGNPGYPG 883

  Fly   990 AK---GDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLD 1051
            .|   |..|..|..||.|..|..||||..||.|:            .|..|.||..|.:|..|..
Zfish   884 PKGLPGPCGQNGCPGNIGQTGCLGPPGPKGLKGL------------NGCSGFPGFNGTKGQKGCV 936

  Fly  1052 GPPGLPGDA---SEKGQKGEPGPSGL---------RGDTGPAGTPGWPGEKGLPGLAVHGRAGPP 1104
            ||||.|||.   ..||:.|.|||:|.         :||.||.||||:||..|.||.. .|..|||
Zfish   937 GPPGFPGDLFYHPVKGEIGPPGPNGENGFCGERGDKGDPGPPGTPGYPGLNGPPGFE-RGCRGPP 1000

  Fly  1105 GEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAG------- 1162
            |.:      |:.|..|:.|..|.:|:.|..|:.|:.|..|..|:||.||..|..|..|
Zfish  1001 GAR------GVPGEPGVRGPHGPKGIVGFPGENGDNGDPGLSGMPGTPGYRGYDGPKGDSGESIC 1059

  Fly  1163 APGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGD 1227
            .||..|..|..||.|.||..|..|..|:.||.|..|..|.|||.|..|..|.||:
Zfish  1060 TPGRTGERGPCGDNGVPGPCGERGDNGDIGPAGPDGNCGPPGPPGINGHIGPPGV---------- 1114

  Fly  1228 KGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPG 1292
               .|..|..|..|.:|..|..||.|..|.:|..||:||||..||:|:.|.|   |.:||||..|
Zfish  1115 ---VGPPGTCGRHGLEGPPGPPGPPGSNGNEGCTGLRGPPGPPGLHGLDGLK---GVKGEIGSRG 1173

  Fly  1293 VTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGER------G 1351
            :...||:|..|..|..|.||..|:.| ..:.|:.|..|..||.|.|||:||.|..||.      |
Zfish  1174 LGSTGERGDQGPEGDKGDQGDPGSRG-PSQTGVRGPKGPQGLQGDPGPMGPPGIVGEECRVPMPG 1237

  Fly  1352 LAGSPGQPGQDGFPGAPGLKGD----------------TGPQGFKGERGLN------GFEGQKGD 1394
            |.|..|.||.||.||..||.||                .|..|||||:|.:      |.:|.:|.
Zfish  1238 LGGERGFPGLDGPPGPMGLMGDPGNAFTVCGECGDPGGPGSLGFKGEKGCHGSGGTPGCQGPQGP 1302

  Fly  1395 KGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPG 1459
            ||::||.|..|:|||.|..||.      |..||.||.|::|.|||||.         ||...:|.
Zfish  1303 KGEKGLCGLMGIPGLPGPMGDC------GPLGPSGAKGDKGDTGPKGD---------PGNVIKPT 1352

  Fly  1460 MLPPP-GPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGE 1523
            ..||| ||.|:||.||:   .||.|:.|:.|..|.:||:||:|..|:.|:||..|..||.|:.|:
Zfish  1353 TCPPPQGPPGDPGPPGQ---IGEVGKNGDNGSGGPKGEKGERGIIGIFGQTGPPGEDGPLGNPGD 1414

  Fly  1524 PGER---GYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLY 1585
            .|||   |::|..|..|.:|.||.....:    ||.|:..||||..||.|.||.|:||.||||||
Zfish  1415 QGERGPAGFKGPPGPDGDRGPPGRNETLS----TGFLLVMHSQSRYVPTCPAGLTQLWNGYSLLY 1475

  Fly  1586 VDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIR 1650
            ::|.:.||.||||..|||:|.|||:| .||...:.|:|||||||::||:|||.||..|::..:|.
Zfish  1476 LEGQERAHTQDLGQAGSCLPVFSTMP-FSCCNMDTCDYASRNDKSYWLSTNAPIPNKPLKGQDIE 1539

  Fly  1651 QYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLED 1715
            ::||||||||||...||:|||....|.||..|..||.|:||:|:|..|:.||||:|.|.||||:|
Zfish  1540 EHISRCVVCEAPTPTIAIHSQDRLDPVCPPKWRSLWTGFSFMMYTGSGDEGGGQSLTSTGSCLQD 1604

  Fly  1716 FRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSH--VSRCQVCMKN 1777
            ||:.||:||.|.:|||.::.::.|||| .::.......|....| ..|||..  .|||.:||.|
Zfish  1605 FRSQPFVECQGPRGTCSYFASIYSFWM-TIDMEHNDSSPHGPVI-TEERQQRDSTSRCSICMMN 1666

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	26/56 (46%)
		Collagen	322..380	CDD:189968	31/69 (45%)
		Collagen	413..465	CDD:189968	20/52 (38%)
		Collagen	499..561	CDD:189968	28/62 (45%)
		Collagen	574..632	CDD:189968	19/63 (30%)
		Collagen	657..714	CDD:189968	23/58 (40%)
		Collagen	765..824	CDD:189968	23/58 (40%)
		Collagen	854..911	CDD:189968	32/59 (54%)
		Collagen	884..943	CDD:189968	26/61 (43%)
		Collagen	923..982	CDD:189968	25/58 (43%)
		Collagen	1028..1085	CDD:189968	27/68 (40%)
		Collagen	1229..1287	CDD:189968	23/57 (40%)
		Collagen	1318..1376	CDD:189968	29/79 (37%)
		Collagen	1399..1458	CDD:189968	24/58 (41%)
		Collagen	1477..1534	CDD:189968	25/59 (42%)
		C4	1555..1662	CDD:128421	62/106 (58%)
		C4	1663..1777	CDD:128421	52/115 (45%)
col4a4	XP_009290110.1	Collagen	259..316	CDD:189968	26/59 (44%)
		Collagen	850..909	CDD:189968	25/58 (43%)
		Collagen	998..1056	CDD:189968	23/63 (37%)
		Collagen	1377..1436	CDD:189968	25/58 (43%)
		C4	1446..1549	CDD:279721	59/103 (57%)
		C4	1555..1664	CDD:279721	50/110 (45%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.720