Protein Alignment Col4a1 and col4a1

DIOPT Version :10

• Sequence 1: NP_723044.1
• Gene: Col4a1 / 33727
• FlyBaseID: FBgn0000299
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_694040.5
• Gene: col4a1 / 554269
• ZFINID: ZDB-GENE-081105-114
• Length: 1640
• Species: Danio rerio
• Alignment Length: 1845
• Identity: 797/1845 - (43%)
• Similarity: 966/1845 - (52%)
• Gaps: 342/1845 - (18%)

  Fly    51 IDDSYDIVDSAGVARGDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPS 115
            ||:|        .|:|.....:|    .||  .|   .|.:|..|.:|..||.|.:|||||:
Zfish    17 IDES--------TAKGGCSGSSC----GGC--DC---SGVKGAKGEVGLPGLMGLVGFPGMQ--- 61

  Fly   116 GDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGL 180
               |.:|.||..|.:||:||.|:||:.|..|:||..|..|.||.||:.|.:|..|..||||..|.
Zfish    62 ---GHEGPPGSMGPKGDRGEIGAPGIKGSRGLPGQAGFPGRPGIPGLPGLEGAPGPQGIPGCNGT 123

  Fly   181 SGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPG--------WRGTAGLAGPQGFPGEKGE 237
            .|..|..|..|..|..|..|.|.      .||.||:||        .:|..||.|..||||:||.
Zfish   124 KGERGFDGIPGITGLLGPPGIPG------IKGGKGDPGGVIGLPIPLKGDRGLPGQHGFPGQKGN 182

  Fly   238 RGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQ 302
            .|..||.|..||              |||  ||.||..|:||||....      |.:|.:|:.||
Zfish   183 AGQQGPVGPPGP--------------YGP--PGRPGDPGQKGEPGDKL------TFIGEKGEKGQ 225

  Fly   303 K---GEPGLVGRKGE----------PGPEGDTGLDGQKGEKG--LPGGPGDRGRQGNFGPPGSTG 352
            :   |.||..||..|          |||.|:||..|.||:||  .|...|.:|..|..||.|..|
Zfish   226 RGLTGPPGPPGRSEEYEGPATTLYLPGPPGETGSTGDKGDKGSCAPHQHGIKGEPGPPGPIGKPG 290

  Fly   353 QKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGP 417
            :.|..||.|..|.|||||..|..|..|..         ||||.|.|:||||||.   |..||.|.
Zfish   291 KDGQSGEKGEIGFPGNPGLDGFKGDKGER---------GPPGYGAGSPGPPGPP---GLPGAEGE 343

  Fly   418 QGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLN--GPKGSIGPIGHPGPPGP 480
            .|..|..|.|||.|    :...|.||.||..|..|||||||..|.:  ||||:.|..|.||||||
Zfish   344 TGFQGEPGAPGPPG----RYIEGPPGPPGFPGEIGKKGEKGADGRSFPGPKGTDGQPGPPGPPGP 404

  Fly   481 --------EGQKGDAGLPG-YGIQGSKGDA----------GIPGYPGLKGSKGERGFKGNAGAPG 526
                    :|..|..|.|| .|..|.|||.          |.||.||.:|.||.:||.|.||..|
Zfish   405 INEECDVTKGAPGPPGPPGLQGEVGQKGDQGETCTECNAFGPPGLPGPQGPKGLQGFPGPAGIKG 469

  Fly   527 DSKLGRPGTP-GAAGAPGQKGDAGRPGTPGQKGDM----GIKGDVGGKCSSCRAGPKGDKGTSGL 586
            :..|..|..| |.||..|..|..|:||..|:.||:    |:|||.|      ..|..||:|..|:
Zfish   470 EKGLPGPSGPAGNAGFNGAPGLMGKPGAQGEPGDIFVAPGLKGDKG------LPGTTGDRGFPGV 528

  Fly   587 PGIPGKDGARGPPGERGYP------GERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCD 645
            .|:|||||..|.||.:|.|      |:||.||..|..|||||:|..|..|: |..|:||.
Zfish   529 DGLPGKDGRPGYPGTKGEPAKFGIKGDRGPDGDFGVPGPPGERGPPGEPGI-GRPGQPGD----- 587

  Fly   646 LSLIEPLKGDKGYPGAPGAKGVQGFKGAEG----LP---GIPGPKGEFGFKGEKGLSGAPGNDGT 703
                   ||..|:|||||..|:||.||..|    ||   |.|||:||.|..|.:|..|.||..|.
Zfish   588 -------KGSAGFPGAPGRPGLQGAKGEAGKVISLPGPQGAPGPRGESGRPGLQGDRGFPGERGV 645

  Fly   704 PGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAK----GNK 764
            ||..|..|.||:||..:.|.||..|..|..|..|..|.|||.|:.|        :|.:    |.|
Zfish   646 PGFPGDKGDPGLPGIGLPGPPGPKGYSGIPGPSGIPGESGEPGQDG--------LPGRAGTPGQK 702

  Fly   765 GEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGK 829
            ||||: |:|||.|..|.||..|:.|.|||||..|.||.||..|..||:|.|||      ||.||.
Zfish   703 GEPGR-GLPGPKGTTGLPGVPGFPGEKGNTGMPGVPGTEGRTGPPGPQGLKGN------PGPPGV 760

  Fly   830 DGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGD 894
            .|..|.||    |...||||  .||||||||           || |||      |..|..||:|.
Zfish   761 HGALGPPG----PPGLGEPG--APGPMGPPG-----------GP-GPI------GQSGIKGDKGY 801

  Fly   895 AGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRG 959
            .||||:.                   :.|||               |:.||||:.|:||:||..|
Zfish   802 QGLPGLD-------------------MPGPP---------------GDRGSPGVPGLPGSKGLPG 832

  Fly   960 APGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQ 1024
            .||..|..||      ||:|||.|..|:.|..|..|..||.||.|..|.:|.||.||:.|..||.
Zfish   833 QPGVPGKDGF------PGERGPKGEIGIMGMAGPPGFQGLVGNPGDPGQKGDPGMPGIRGDFGDL 891

  Fly  1025 GLAGAPGQQGLDGMPGEKGNQGFPGLDGPPG---------LPGDASEKGQKGEPGPSGLRGDTGP 1080
                           |.||.:|.|||.||||         :.||..:.|.:|:|||:|.:|..|.
Zfish   892 ---------------GTKGERGEPGLQGPPGNMSDVDMEHMKGDKGDIGDRGDPGPTGEKGFPGT 941

  Fly  1081 AGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGA 1145
            :|.||.||:.|:|        |.||:.||:|..||.|:.|..||.|:.|.:|..|.||..|..||
Zfish   942 SGDPGMPGKDGMP--------GSPGQPGDKGDPGIIGKPGSIGEPGDTGARGDMGYPGSMGPKGA 998

  Fly  1146 PGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERG 1210
            .|:.|:||..|..|..|:      .||:||.||||: |:||..|..|.:|..||.|.||.||::|
Zfish   999 KGVGGSPGHPGFKGTDGS------KGDKGDPGEPGI-GIPGPPGTKGNIGSPGFPGEPGEKGQKG 1056

  Fly  1211 IRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGI 1275
            ..|..|.|.|    :|.||.||..||.|..|:.||:|:   .|:.|:.|:.|..|.||..||.|.
Zfish  1057 TMGMTGTPGT----QGHKGDQGSIGYPGSPGKPGEKGV---GGLPGSPGEPGTPGRPGEPGLQGP 1114

  Fly  1276 PGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGP 1340
            ||..|:.|..|:.|.||.|  ||:|.||.|||       |.||..||.|..|..|.|||.|:||.
Zfish  1115 PGPHGEKGQSGQDGIPGFT--GERGEPGVPGR-------GLPGSRGEPGGKGDKGNPGLPGIPGS 1170

  Fly  1341 IGPAGSKGERGLAGSP---GQPGQDGFPG--APGLKGDTGPQGFKGERGLNGFE---------GQ 1391
            .|..|:||::||:|.|   |:||:.|.||  ..|.|||.|.||..||||..|.:         |.
Zfish  1171 PGIPGTKGDKGLSGLPGDQGRPGERGLPGQAMEGPKGDRGDQGQPGERGATGEQGPPGIPGSAGS 1235

  Fly  1392 KGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKG 1456
            ||:|||.|.|   |.||..|.|||.|:.|::|.      ||..|:.||||..|..|.||.||.||
Zfish  1236 KGEKGDVGFQ---GAPGSRGYKGDKGFIGVSGE------PGLPGYNGPKGEMGPQGVPGFPGTKG 1291

  Fly  1457 EPGMLPP---------PGPKGEPGQPGRNGP----KGEPGRP---GERGLIGIQGERGEKGERGL 1505
            |.|::..         ||.||..|.||..||    ||:||.|   |.:||:||||..|.||::|:
Zfish  1292 EQGVIGSKGEGGDRGFPGLKGSEGPPGPPGPHTYVKGDPGPPGPQGPQGLVGIQGFPGAKGQQGV 1356

  Fly  1506 I---------GETGNVGRPGPKGDRGE---PGERGYEGAIGLIGQKGEPGAPAPAALDYLTGILI 1558
            :         |..|..|.||.||:.|:   ||.|||.|..|..|..|..|.|.|:::|:  |.|:
Zfish  1357 MGLQGLKGSDGTPGYNGHPGAKGEPGQTGPPGPRGYPGPPGPDGIPGHIGPPGPSSMDH--GFLV 1419

  Fly  1559 TRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNY 1623
            |||||:..||.|..|.|.::.|||||||.||:.:|.||||:.|||:.:||.:|.|.|..|||||:
Zfish  1420 TRHSQTIDVPHCPQGTTPIYDGYSLLYVQGNERSHGQDLGTAGSCLRKFSPMPFLFCNINNVCNF 1484

  Fly  1624 ASRNDKTFWLTTNAAIP--MMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLW 1686
            |||||.::||||...:|  |.||....|:.:||||.||||||.|||||||||::|:||:||..||
Zfish  1485 ASRNDYSYWLTTPEPMPMSMAPVTGESIKPFISRCAVCEAPAMVIAVHSQTIQIPNCPDGWASLW 1549

  Fly  1687 IGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPF 1751
            |||||:|||:.|..|.||||.|||||||:||:.|||||:| :|||::|....|||:..:|.:..|
Zfish  1550 IGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHG-RGTCNYYANSYSFWLATIEDTDMF 1613

  Fly  1752 ERPQQQTIKAGERQSHVSRCQVCMK 1776
            .:|...|:|||..::|:||||||||
Zfish  1614 TKPVPATLKAGSLRTHISRCQVCMK 1638

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	gly_rich_SclB	<107..>361	CDD:468478	110/276 (40%)
		gly_rich_SclB	<355..>642	CDD:468478	139/318 (44%)
		gly_rich_SclB	<543..820	CDD:468478	131/297 (44%)
		gly_rich_SclB	<727..>968	CDD:468478	96/244 (39%)
		gly_rich_SclB	<969..>1218	CDD:468478	105/257 (41%)
		gly_rich_SclB	<1186..>1420	CDD:468478	111/247 (45%)
		gly_rich_SclB	<1321..>1547	CDD:468478	116/267 (43%)
		C4	1555..1662	CDD:128421	60/108 (56%)
		C4	1663..1777	CDD:128421	69/114 (61%)
col4a1	XP_694040.5	gly_rich_SclB	<40..>211	CDD:468478	82/198 (41%)
		gly_rich_SclB	<296..>542	CDD:468478	116/267 (43%)
		gly_rich_SclB	<469..>737	CDD:468478	127/295 (43%)
		gly_rich_SclB	<700..>972	CDD:468478	148/365 (41%)
		gly_rich_SclB	<890..>1142	CDD:468478	119/290 (41%)
		gly_rich_SclB	<1056..>1290	CDD:468478	115/258 (45%)
		C4	1417..1524	CDD:460201	58/106 (55%)
		C4	1527..1638	CDD:460201	66/111 (59%)