DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col4a6

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_017212581.1	Gene:	col4a6 / 554268	ZFINID:	ZDB-GENE-101001-2	Length:	1717	Species:	Danio rerio

Alignment Length:	1829	Identity:	796/1829 - (43%)
Similarity:	943/1829 - (51%)	Gaps:	266/1829 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 KNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGF---PGMEGPSGDKGQKGDPGPYGQRGD 132
            ::|    :|..| |...||.||.||.|||.|..|..||   ||..||..::|..|..||.||.||
Zfish    30 RDC----SGPCP-CFPLKGGRGQPGELGPVGSGGPEGFYGGPGPVGPKAEQGHIGRKGPPGQNGD 89

  Fly   133 KGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKG 197
            ||..|.||..|..||||..|..|..|.||::|.:|..|..|..||:|.||:||..|:.|
Zfish    90 KGPMGVPGYPGNDGVPGHPGQEGPRGPPGLDGCNGTQGDTGYSGLDGYSGIPGSPGFPG------ 148

  Fly   198 EKGEPAKENGDYAKGEKGEPGWRG-------TAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGL 255
                        .||:||||.:..       |.|..||||:|         ||.|.:||.|..||
Zfish   149 ------------LKGQKGEPSYLNIPSQNILTQGPPGPQGYP---------GPIGPRGPDGFDGL 192

  Fly   256 KGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMG-----------QKGEPGLV 309
            .|..|.    |..||:|| :|.||||.....|.|    .|.||:..           .|||.|.|
Zfish   193 PGLPGP----PGPPGSPG-QGYKGEPGEKGDVGP----QGVRGNPSVQFNAPAISTINKGEKGDV 248

  Fly   310 GRKGEPG-------------PEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQK------G 355
            |..|:||             |:.|.|:.|.||..|.||.||.:|....|..||..|:|      |
Zfish   249 GPPGDPGHPLVNGVVEFVGIPKADKGIQGDKGYAGNPGLPGRQGPVNPFRYPGEVGEKGILGLPG 313

  Fly   356 DRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGP------------KGP 408
            .||.|||||.||.||:||..|..|..|:|||.||.    |..|.||||||            ||.
Zfish   314 GRGPPGLNGPPGLPGRKGFTGSEGFPGQPGLNGPK----GDIGDPGPPGPIVDSPVFEIKGSKGD 374

  Fly   409 RGYVGAPGPQGLNGVDGLPGPQGYNGQ----KGGAGLPGRPGNEGPPGKKGEK-----GTAGLNG 464
            ||:.|.||..|..|..||||..|..|.    .|..|.||.||:.||.|::|..     |:.||.|
Zfish   375 RGFPGYPGFPGDTGYGGLPGQPGAPGPPAFFTGRPGAPGFPGSRGPKGQRGNPGRDSYGSEGLPG 439

  Fly   465 PKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSK 529
            .:|..||.|.|||||.........|||:           ||.||.:|..|.:|.||..|..||..
Zfish   440 SQGLSGPPGLPGPPGTSSNFIVTPLPGF-----------PGQPGPRGPFGSQGIKGFKGENGDCN 493

  Fly   530 LGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDG 594
            ....||.||.|.||.      ||.||..|..|:||::            ||.|..||||..|..|
Zfish   494 CLGGGTIGAQGPPGP------PGFPGFIGLNGLKGEI------------GDNGYQGLPGPQGPQG 540

  Fly   595 ARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYP 659
            .|   |:||:.|.:|..|........|:||:.|:.|.||                 ||    |:.
Zfish   541 RR---GDRGFNGRKGEKGNAFFPQGKGQKGDLGQQGPPG-----------------PL----GFS 581

  Fly   660 GAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRD--GYPGIPG-QSIK 721
            .:||..|..||.|..||||.||    ||..|:||..|.||:.|.||.:|..  ||||:.| :.::
Zfish   582 NSPGRDGSPGFPGQPGLPGEPG----FGLTGDKGFPGIPGSKGRPGASGEPGIGYPGVAGFKGMQ 642

  Fly   722 GEPGFHGRDGAKGDKGSFGRS-----------GEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGP 775
            |||||:|..|..|..||.|.|           .|.|.|..|.|..|    :|:||.||:.|:|||
Zfish   643 GEPGFNGLPGPPGPPGSKGDSCRQAGLIDASLEEGGTPLPCRLPGI----QGDKGFPGRPGVPGP 703

  Fly   776 PGEDGSPGERGYTGLKGNTGPQGPPGV---EGPRGLNGPRGEKGNQGA--VGVPGNPGKDGLRGI 835
            .|..||.|..|..|..|:.|.:|.||.   .||:|..||||:.|..|.  :|:.|:||:||.
Zfish   704 KGRVGSAGLNGRPGFDGSKGERGEPGSGGRPGPQGFPGPRGDAGLPGTTNIGLQGSPGRDGF--- 765

  Fly   836 PGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGV 900
               .|.|||:||||.......|..|..|..||.||:|..|..|||      |.||..|..|.||:
Zfish   766 ---PGSPGPKGEPGAVLGATPGDLGRQGRPGEPGDKGQPGVPGFP------GNPGVDGRNGFPGI 821

  Fly   901 SGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDG 965
            .|..|:.|..|.||||||.|.. |.|  ||.|.||..||||..|.||..|.||.||.||.||..|
Zfish   822 KGERGVNGLPGPVGPIGPPGEL-PQG--GIPGPRGPPGAKGFDGPPGCQGFPGLKGMRGDPGPPG 883

  Fly   966 P---KGFAGVTGAPGK---RGPA---------GIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAP 1015
            |   ||..|:.|.||.   |||.         ||||..|.||:||:.||.|..|..|..|.||.|
Zfish   884 PGGIKGIQGIPGIPGSQGFRGPPGPTGFTERDGIPGRPGIKGEKGSLGLLGFQGLPGSPGIPGGP 948

  Fly  1016 GLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGP--PGLPGDASEKGQKGEPGPSGLRGDT 1078
               ||||..|..|..|..|.||..|.:|::|.||..||  |.:|.:...||:.||||..|..|.|
Zfish   949 ---GIKGLPGFRGQDGLIGRDGPRGPRGDRGEPGPPGPSTPPIPVERLVKGEPGEPGIVGGPGFT 1010

  Fly  1079 GPAGTPGWPGEKGLPGLAVHGRAGPPG----EKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGE 1139
            |..|..|:||..|.||.     .||||    .||..|:.||.|..|.:|..|.:|..||.|.||.
Zfish  1011 GQRGDKGFPGPPGRPGF-----PGPPGFADQAKGLPGQPGIPGVPGSSGFPGPKGFPGVMGFPGF 1070

  Fly  1140 KGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGD-------RGDKGEPGLSGLPGLKGETGPVGLQ 1197
            .|..|..|:||.|   |.|||.|.|||.|..||       .|.||..|.||.||..|..|..|..
Zfish  1071 SGVKGDNGVPGVP---GFPGAQGPPGAKGEKGDSYASSGPTGPKGNIGNSGFPGGPGRPGEPGDS 1132

  Fly  1198 GFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRG 1262
            ||.|..|.:|::|..|.||:|..|    |..|..|.||..|..|.:|..|..|..|..|..||:|
Zfish  1133 GFPGVLGYQGQKGFPGDPGIPGFV----GSSGPPGPRGLPGYPGLRGRDGDPGRPGGPGLPGDKG 1193

  Fly  1263 LQGPPGASGLNGIPGAKGDIG-----PRGEIGYPGVT-IKGEK-----GLPGRPGRNGRQGLIGA 1316
            ..|.||..||||:||::|..|     ..|.:|.|||. .|||:     |:|||||..|.:|..||
Zfish  1194 FPGSPGLDGLNGLPGSRGPPGVPGVIEGGRVGPPGVNGQKGERGTSQPGVPGRPGEKGVRGDTGA 1258

  Fly  1317 PGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKG 1381
            .||.|..|.||..|:|.|..:|||:|..|..|..|..|..|.||..|.||...::||.|..||.|
Zfish  1259 RGLRGYPGNPGPPGQPSLSNIPGPLGDPGLPGLDGREGPFGPPGLPGPPGPGTVQGDRGDPGFPG 1323

  Fly  1382 ERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRD 1446
            ..|:.|..|:.|..|..||||.   ||..||:|:.|:.|:.|..|..|.||:.|.:||:|..|..
Zfish  1324 RPGIIGPRGEPGGFGAPGLQGN---PGFKGQRGEPGFSGVPGRPGSPGEPGDYGRSGPRGLKGTS 1385

  Fly  1447 GTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGN 1511
            |..|.||...... :....|.|:|||||.:|..|:||.|   ||.|..|..|.||.||.:|..|.
Zfish  1386 GPVGNPGFVFASN-ITARMPSGDPGQPGLDGFAGQPGIP---GLPGSPGNPGPKGFRGQVGRLGP 1446

  Fly  1512 VGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPA-PAALDYLTGI--LITRHSQSETVPACSAG 1573
            ||..|..|..|:||..|.:|..||.|.:|:||.|. ||.....|||  .:.:||||..||.|..|
Zfish  1447 VGLAGSPGFTGDPGPSGIQGPTGLQGPRGKPGLPGIPAPPTRSTGIGYTLVKHSQSTRVPMCPEG 1511

  Fly  1574 HTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAA 1638
            .|.||.|||||||:|.:.|||||||.||||:|.|||:|.|.|..|.:|.|||||||::||:|.||
Zfish  1512 MTRLWEGYSLLYVEGQEKAHNQDLGLPGSCLPHFSTIPFLYCTANEICYYASRNDKSYWLSTTAA 1576

  Fly  1639 IPMMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGG 1703
            ||||||.|.||..|||||.|||.|:..||||||.:.:|.||.||..|||||||||||..|..|||
Zfish  1577 IPMMPVANQEISPYISRCSVCEVPSQPIAVHSQDLTIPTCPQGWRSLWIGYSFLMHTGAGGEGGG 1641

  Fly  1704 QALQSPGSCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPF-ERPQQQTIKAGERQSH 1767
            |:|.||||||||||||||||||||:||||::....|||:..::.::.| :.|.|:|:|..:.:|.
Zfish  1642 QSLMSPGSCLEDFRATPFIECNGARGTCHYFANKYSFWLTTVDVNEQFVQGPTQETLKGDQFRSR 1706

  Fly  1768 VSRCQVCMK 1776
            :||||||||
Zfish  1707 LSRCQVCMK 1715

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	28/59 (47%)
		Collagen	322..380	CDD:189968	30/63 (48%)
		Collagen	413..465	CDD:189968	24/60 (40%)
		Collagen	499..561	CDD:189968	24/61 (39%)
		Collagen	574..632	CDD:189968	21/57 (37%)
		Collagen	657..714	CDD:189968	27/58 (47%)
		Collagen	765..824	CDD:189968	28/63 (44%)
		Collagen	854..911	CDD:189968	22/56 (39%)
		Collagen	884..943	CDD:189968	29/58 (50%)
		Collagen	923..982	CDD:189968	33/64 (52%)
		Collagen	1028..1085	CDD:189968	25/58 (43%)
		Collagen	1229..1287	CDD:189968	25/62 (40%)
		Collagen	1318..1376	CDD:189968	25/57 (44%)
		Collagen	1399..1458	CDD:189968	25/58 (43%)
		Collagen	1477..1534	CDD:189968	25/56 (45%)
		C4	1555..1662	CDD:128421	70/108 (65%)
		C4	1663..1777	CDD:128421	69/115 (60%)
col4a6	XP_017212581.1	None

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	161	1.000	Domainoid score	I3989
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1311	1.000	Inparanoid score	I148
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	1	1.000	-	-		mtm6613
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	10	9.870