DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col4a2

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_017213247.1	Gene:	col4a2 / 553355	ZFINID:	ZDB-GENE-070725-3	Length:	1670	Species:	Danio rerio

Alignment Length:	1807	Identity:	773/1807 - (42%)
Similarity:	956/1807 - (52%)	Gaps:	256/1807 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    60 SAGVAR--GDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPL---GPTGLKGEMGFPGMEGPSGDKG 119
            :|||.:  |....::|:   .||  :|..|||.|||||||   ||||.||..|.||::||.|.||
Zfish    30 NAGVKKSTGPCGGRDCS---GGC--QCYPEKGARGLPGPLGPQGPTGPKGRQGEPGLQGPKGMKG 89

  Fly   120 QKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMP 184
            :.|:.|..|.:|..|..|.||.:|..||||..||:   ||.|..|.|||:|..|..|:.|..|:.
Zfish    90 EHGEAGFVGPKGSVGMEGVPGFNGADGVPGHPGPS---GARGKAGPDGCNGTRGDSGMPGFPGLE 151

  Fly   185 GPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGP 249
            |.:|..|..|.|||||:|. |...|.:..:|:||..|..|:.||.|.||.:|..|..||:|.:|.
Zfish   152 GGQGTPGWPGMKGEKGDPL-EVWVYMERFRGDPGPAGFPGIVGPTGDPGYRGLPGFQGPWGPQGL 215

  Fly   250 RGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTV----MGPRGDMGQKGEPGLVG 310
            :|..|.|||:     ..:..|..|..||.|||........|..:    .|.:|.||.||:.|.:|
Zfish   216 KGAKGQKGER-----TKILKGVKGELGEIGEPGPPGVFSQTSPIPSDWQGEKGKMGSKGDKGDLG 275

  Fly   311 RKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGL----------NGL 365
            ........|.:|..|.:|..||.|.||.||..|..|.||:.|:|||.||||.          .|.
Zfish   276 YLSTKAESGVSGFAGARGNPGLDGWPGPRGDTGLPGFPGNNGRKGDVGEPGELVGSPFDDTGVGP 340

  Fly   366 PGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTP---GPPGPKGPRGYVGAPGPQGLNGVDGLP 427
            ||.||::|..|..|..|..|..||||||..|:.|.   ||.||:||:|..|..|..|.....  |
Zfish   341 PGPPGERGPVGNYGRKGAKGQPGPPGPPPYGQQTVELWGPEGPRGPKGASGETGEPGNPATQ--P 403

  Fly   428 GPQGYNGQKGGAGLPGRPGN-----EGPPGKKGEKGTAGLNGPKG-------SIGPIGHPGPPGP 480
            ||.|.:|..|..|.||..|:     :|.||.:|..|:||..||||       |:.|  .||||||
Zfish   404 GPPGPDGSPGSVGPPGPKGSLEEYFKGSPGHRGRPGSAGKKGPKGDHGLCECSVKP--PPGPPGP 466

  Fly   481 EGQKGDAGLPG-YGIQGSKGDA------GIPGYPGLKGSKGERGFKG------NAGAPGDSKLGR 532
            .|..||.|:.| :|.||.:||.      |:||:||.:|..|.:|.||      ..|:.||
Zfish   467 PGDSGDPGMAGEWGQQGDQGDPGTKGANGLPGFPGTEGLPGPKGRKGELMEAVQKGSAGD----- 526

  Fly   533 PGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARG 597
            ||.||.:|.||::   ||||..|:.|:.|.:|..|       .||.|:.|..|..|.||:.|..|
Zfish   527 PGDPGHSGFPGEQ---GRPGIDGRDGEPGFRGPPG-------EGPVGEPGAKGYSGPPGRSGLIG 581

  Fly   598 PPGERGYPGE--RGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPG 660
            |   :|.||:  ....|::|..|..|:.|.|||||.||..|:.........|:.....||   ||
Zfish   582 P---KGNPGQVLGATKGLSGLPGDDGQTGPDGRTGPPGPPGDCSSRGGSQWSIPVECIGD---PG 640

  Fly   661 APGAKGVQGFKGAEGLPGIPGPKGEFGFK---GEKGLSGAPGNDGTPGRAGRD------GYPGIP 716
            .||..|:.|.:|.||.||:|||||..||.   |:||..|.||..|.||..|..      ||||..
Zfish   641 PPGESGLPGPRGLEGFPGVPGPKGTAGFSGDLGDKGERGEPGRGGPPGPKGFTGPRGDLGYPGTK 705

  Fly   717 GQSIKGEPGFHGR---DGAKGDKGSFGR-----SGEKGEPGSCALDEIKMPAKGNKGEPGQTGMP 773
            |  :||.||..|:   |||.|.||..|.     ||..|:||        :|  |::|:.|.||.|
Zfish   706 G--VKGPPGLSGKPGLDGASGGKGEHGEVFGASSGAPGDPG--------LP--GHRGDTGLTGDP 758

  Fly   774 GPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGR 838
            |.||..|..|..|.|||||.:   ||||::|.:|..||      .|..|.||..|..|..|.||.
Zfish   759 GLPGYFGMEGMPGMTGLKGES---GPPGLQGEQGRPGP------PGTFGFPGQTGYTGPPGPPGN 814

  Fly   839 NGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGR 903
            .|:|||.|     |.|..|.|||.|.:|.||..|..|.|   |..|::|.|||:|:.|.||..|.
Zfish   815 IGEPGPSG-----RRGDQGEPGLIGARGVKGAIGDPGNI---GVTGNIGPPGDQGETGGPGFFGL 871

  Fly   904 PGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKG 968
            ||:.|.||.      .|:.|.||..|..|::|..|.|||      :|:||::|.:||||:.|.||
Zfish   872 PGLKGSKGG------QGMTGFPGKTGERGLKGFSGTKGE------IGLPGHQGAKGAPGSPGQKG 924

  Fly   969 FAGVTGAPGKRGPAGIPG-----VSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAG 1028
            ..|:||.||.:....|..     :.|:|||.      ||.|..|..||.|..|..||.|.:|..|
Zfish   925 DRGLTGPPGDKPVIHITPHMKEVMKGSKGDH------GNMGDPGFTGPRGTKGFPGIPGGEGKDG 983

  Fly  1029 APGQ----QGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGE 1089
            .||:    :|:.|:|||      |||:||            ||.|||:||            ||.
Zfish   984 HPGEPSVVKGVKGLPGE------PGLEGP------------KGMPGPTGL------------PGI 1018

  Fly  1090 KGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGM 1154
            :|.||:     :||.|.||..|..|..|..||.|.||:.|           .::..||..|..|.
Zfish  1019 EGFPGM-----SGPKGNKGSSGAYGRPGDSGIKGFKGDLG-----------PTISLPGSTGLRGE 1067

  Fly  1155 DGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPV------GLQGFTGAPGPKGERGIRG 1213
            .|.||:.||.|..|.||:||..|..|:.|:.|.:||.|.|      |||||.|:.|.||.
Zfish  1068 TGHPGSTGAKGLYGMPGERGSSGLDGIEGMKGYQGEPGTVGPPGSDGLQGFPGSQGHKGR----- 1127

  Fly  1214 QPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGAS--GLNGIP 1276
                       .||.|..|..|..|:.|::|..||.|..|:.|.||.:|.||.||..  |..|.|
Zfish  1128 -----------SGDPGQTGTTGIQGQPGQKGFPGLKGIFGLDGLKGQKGNQGVPGTDNWGQPGNP 1181

  Fly  1277 GAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPI 1341
            |.||::   ||.|.|..|    .|:||..|..|..|..|..|.||:||:||..|:.|...:.|..
Zfish  1182 GTKGEL---GETGIPSTT----TGVPGSLGHKGTTGDSGNKGEIGQRGVPGPQGQQGFSDIKGTQ 1239

  Fly  1342 GPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGL 1406
            |..|..|.:|:.|.||..|..|.|...|.|||      :|..|..|..|:||.:||||.:|.||.
Zfish  1240 GDYGFPGIKGIPGFPGTRGTPGIPSPFGAKGD------RGNVGDFGLFGEKGVQGDRGEKGSSGE 1298

  Fly  1407 PGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPP--PGPKGE 1469
            .|:.|:|||.|.||:.|..|..|..|:||..||||..|..|.|||||.||.| .:|.  ||.:|.
Zfish  1299 SGVSGRKGDKGEPGMMGFPGRRGFQGDRGPFGPKGDTGPPGFPGLPGAKGYP-PVPQKLPGEQGP 1362

  Fly  1470 PGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIG 1534
            |||.|..||.|..|.||..|..|..|..|..|.:|:   .|..|.||..|.|||||..|:.|..|
Zfish  1363 PGQMGIQGPSGRNGNPGPAGPPGDSGFVGPSGHKGM---PGLPGIPGSPGFRGEPGSMGHSGLQG 1424

  Fly  1535 LIGQKGEPGAPAP---AALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQD 1596
            ..|.:|.||.|.|   |......|.|:.:|||||.:|.|..|.:.||.||||||.:|.:.|||||
Zfish  1425 EQGTRGRPGMPGPLGMAGRSVNVGYLLVKHSQSEEIPMCPQGMSLLWMGYSLLYFEGQEKAHNQD 1489

  Fly  1597 LGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEA 1661
            ||..|||:|||:|:|.|.|...::|.|||||||::||:|...|||||||..||:.|||||.||||
Zfish  1490 LGLAGSCLPRFNTMPFLYCNPGDICYYASRNDKSYWLSTTQPIPMMPVEESEIKPYISRCSVCEA 1554

  Fly  1662 PANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNG 1726
            |:..||||||...:|:||.||..||||||||||||.|:.||||:|.||||||||||.||||||||
Zfish  1555 PSVAIAVHSQDTTIPNCPVGWRSLWIGYSFLMHTAAGDEGGGQSLASPGSCLEDFRTTPFIECNG 1619

  Fly  1727 AKGTCHFYETMTSFWMYNLESSQPFE-RPQQQTIKAGERQSHVSRCQVCMKN 1777
            ||||||::....|||:.:::.|  |: .|..:|:|||:..|.:|||||||||
Zfish  1620 AKGTCHYFANKHSFWLTSIDES--FQSSPSSETLKAGQLLSRISRCQVCMKN 1669

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	26/56 (46%)
		Collagen	322..380	CDD:189968	29/67 (43%)
		Collagen	413..465	CDD:189968	20/56 (36%)
		Collagen	499..561	CDD:189968	28/73 (38%)
		Collagen	574..632	CDD:189968	24/59 (41%)
		Collagen	657..714	CDD:189968	30/65 (46%)
		Collagen	765..824	CDD:189968	26/58 (45%)
		Collagen	854..911	CDD:189968	26/56 (46%)
		Collagen	884..943	CDD:189968	25/58 (43%)
		Collagen	923..982	CDD:189968	26/58 (45%)
		Collagen	1028..1085	CDD:189968	20/60 (33%)
		Collagen	1229..1287	CDD:189968	24/59 (41%)
		Collagen	1318..1376	CDD:189968	24/57 (42%)
		Collagen	1399..1458	CDD:189968	31/58 (53%)
		Collagen	1477..1534	CDD:189968	24/56 (43%)
		C4	1555..1662	CDD:128421	64/106 (60%)
		C4	1663..1777	CDD:128421	71/114 (62%)
col4a2	XP_017213247.1	Collagen	59..118	CDD:189968	30/58 (52%)
		Collagen	113..169	CDD:189968	28/58 (48%)
		Collagen	495..559	CDD:189968	29/78 (37%)
		Collagen	879..935	CDD:189968	28/67 (42%)
		Collagen	996..1054	CDD:189968	35/103 (34%)
		Collagen	1069..1126	CDD:189968	26/56 (46%)
		C4	1449..1553	CDD:307529	61/103 (59%)
		C4	1559..1667	CDD:307529	69/109 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	161	1.000	Domainoid score	I3989
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H1390
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	1	1.000	-	-		mtm6613
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	7	6.820