DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Col4a1 and COL5A3

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_056534.2	Gene:	COL5A3 / 50509	HGNCID:	14864	Length:	1745	Species:	Homo sapiens

Alignment Length:	1613	Identity:	608/1613 - (37%)
Similarity:	722/1613 - (44%)	Gaps:	400/1613 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    60 SAGVARGDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMG-------FPGMEGPSGD 117
            :|.:....|.|.:.|.  .|.:....|.:...|....:||.....|..       |||    :|:
Human   316 NATILERSLDPDSGTE--LGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPG----AGE 374

  Fly   118 KGQKGDPGPYGQRGDKGER--GSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGL 180
            ||.||:|...    :||::  |.||..|..||.|..||.|.||.||..|..|..|..||||::|:
Human   375 KGAKGEPAVI----EKGQQFEGPPGAPGPQGVVGPSGPPGPPGFPGDPGPPGPAGLPGIPGIDGI 435

  Fly   181 SGMPG-----PRGYAGQLGSKGEKGEPAKENGDYAK--------GEKGEPGWRGTAGLAGPQGFP 232
            .|.||     |..:||  ||  .||.|.......|:        ..||.||..|..|..||.|.|
Human   436 RGPPGTVIMMPFQFAG--GS--FKGPPVSFQQAQAQAVLQQTQLSMKGPPGPVGLTGRPGPVGLP 496

  Fly   233 GE---KGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVM 294
            |.   |||.|..||.|.:|.:|.||..|..|       |.|.||..|.:|.|..:
Human   497 GHPGLKGEEGAEGPQGPRGLQGPHGPPGRVG-------KMGRPGADGARGLPGDT---------- 544

  Fly   295 GPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGE 359
            ||:||.|..|.|||.|.||:   .||.|..||      ||.||:.|.:|..||||.|||.|:.|.
Human   545 GPKGDRGFDGLPGLPGEKGQ---RGDFGHVGQ------PGPPGEDGERGAEGPPGPTGQAGEPGP 600

  Fly   360 PGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVD 424
            .||.|..|:||..|.||..|..|.||..|..|||    |.|||||.:|..|..|.||||||.|..
Human   601 RGLLGPRGSPGPTGRPGVTGIDGAPGAKGNVGPP----GEPGPPGQQGNHGSQGLPGPQGLIGTP 661

  Fly   425 GLPGPQGYNGQKGGAGLPGRPGNEGP---PGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGD 486
            |..||      .|..|:||.||::||   ||.:|..|..|..||.||.||.|:|||.|.:|..|:
Human   662 GEKGP------PGNPGIPGLPGSDGPLGHPGHEGPTGEKGAQGPPGSAGPPGYPGPRGVKGTSGN 720

  Fly   487 AGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRP 551
            .||     ||.||:.|..|:||.||..|.:|.:|..||||..  |..|..|..|..||.|:.|.|
Human   721 RGL-----QGEKGEKGEDGFPGFKGDVGLKGDQGKPGAPGPR--GEDGPEGPKGQAGQAGEEGPP 778

  Fly   552 GTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQ 616
            |:.|:||.:|:.|..|      ..|..|.||:.|.|      |..||.||:|..|:.|..|:.|:
Human   779 GSAGEKGKLGVPGLPG------YPGRPGPKGSIGFP------GPLGPIGEKGKSGKTGQPGLEGE 831

  Fly   617 TGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPG 681
            .||||.:||.|:   |||||:||.            |||.|..||||..|.:|..|.:|.||.||
Human   832 RGPPGSRGERGQ---PGATGQPGP------------KGDVGQDGAPGIPGEKGLPGLQGPPGFPG 881

  Fly   682 PKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKG 746
            ||                  |.||..|:||.||.|||  :||.||.|:.|..|..|..|..|:.|
Human   882 PK------------------GPPGHQGKDGRPGHPGQ--RGELGFQGQTGPPGPAGVLGPQGKTG 926

  Fly   747 EPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGP 811
            |.|..             ||.|..|.||||||.|.||..|..|.||..||.||.|.|||.||.|.
Human   927 EVGPL-------------GERGPPGPPGPPGEQGLPGLEGREGAKGELGPPGPLGKEGPAGLRGF 978

  Fly   812 RGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGP 876
            .|.||.      ||:||..||:|..|..|..|..|.||  ..||:||.|..||.|:.|..||.||
Human   979 PGPKGG------PGDPGPTGLKGDKGPPGPVGANGSPG--ERGPLGPAGGIGLPGQSGSEGPVGP 1035

  Fly   877 IGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKG 941
                     .|..|.||:.|.||.:|:.||   .|.:||:||.|.|||         .|.:|.||
Human  1036 ---------AGKKGSRGERGPPGPTGKDGI---PGPLGPLGPPGAAGP---------SGEEGDKG 1079

  Fly   942 EPGSPGLVGMPGNKGDRGAPGND---GPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGND 1003
            :.|:||..|..|:|||.|.||..   ||.|..|..||.|.:|..|.||:.|.|||.|..|.
Human  1080 DVGAPGHKGSKGDKGDAGPPGQPGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGF---- 1140

  Fly  1004 GPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGE 1068
              ||..||||..||.|..|::|..|..|..|..|.||.:|.||..|.:|.|||||...:.|..||
Human  1141 --VGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPQGPTGSEGTPGLPGGVGQPGAVGE 1203

  Fly  1069 PGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGV 1133
            .|..|..||.||.|.||.||.|           |..|||||.|.||..|..|..|..||.|.:|.
Human  1204 KGERGDAGDPGPPGAPGIPGPK-----------GDIGEKGDSGPSGAAGPPGKKGPPGEDGAKGS 1257

  Fly  1134 WGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQG 1198
            .|..|..|.:|.||.||..|:||.||..|.||.||.||..|..|||
Human  1258 VGPTGLPGDLGPPGDPGVSGIDGSPGEKGDPGDVGGPGPPGASGEP------------------- 1303

  Fly  1199 FTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGL 1263
              |||||.|:||       |:               |:.|.:|.:||:         ||||:.|.
Human  1304 --GAPGPPGKRG-------PS---------------GHMGREGREGEK---------GAKGEPGP 1335

  Fly  1264 QGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGL 1328
            .||||.:         |.:|.||.                 |||.|.:||.|.||.:||   |||
Human  1336 DGPPGRT---------GPMGARGP-----------------PGRVGPEGLRGIPGPVGE---PGL 1371

  Fly  1329 AGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKG 1393
            .|.||.:|.|||:||:                     |.|||||||||               ||
Human  1372 LGAPGQMGPPGPLGPS---------------------GLPGLKGDTGP---------------KG 1400

  Fly  1394 DKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEP 1458
            :||..||.|..|.||..|:|||.|.||:.|
Human  1401 EKGHIGLIGLIGPPGEAGEKGDQGLPGVQG----------------------------------- 1430

  Fly  1459 GMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGE 1523
                ||||||:||.||..|..|.||.||..|.:|.:|.:|..|..|..|:||..|.|||
Human  1431 ----PPGPKGDPGPPGPIGSLGHPGPPGVAGPLGQKGSKGSPGSMGPRGDTGPAGPPGP------ 1485

  Fly  1524 PGERGYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDG 1588
                              |||||.     |.|:...|......:|....|..|:....:.|.::
Human  1486 ------------------PGAPAE-----LHGLRRRRRFVPVPLPVVEGGLEEVLASLTSLSLE- 1526

  Fly  1589 NDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRN-----DKTFWLTTN 1636
                 .:.|..|    |..:..|.|      ||:...||     |..:|:..|
Human  1527 -----LEQLRRP----PGTAERPGL------VCHELHRNHPHLPDGEYWIDPN 1564

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	24/65 (37%)
		Collagen	322..380	CDD:189968	27/57 (47%)
		Collagen	413..465	CDD:189968	24/54 (44%)
		Collagen	499..561	CDD:189968	27/61 (44%)
		Collagen	574..632	CDD:189968	23/57 (40%)
		Collagen	657..714	CDD:189968	21/56 (38%)
		Collagen	765..824	CDD:189968	31/58 (53%)
		Collagen	854..911	CDD:189968	23/56 (41%)
		Collagen	884..943	CDD:189968	23/58 (40%)
		Collagen	923..982	CDD:189968	25/61 (41%)
		Collagen	1028..1085	CDD:189968	26/56 (46%)
		Collagen	1229..1287	CDD:189968	17/57 (30%)
		Collagen	1318..1376	CDD:189968	23/57 (40%)
		Collagen	1399..1458	CDD:189968	14/58 (24%)
		Collagen	1477..1534	CDD:189968	19/56 (34%)
		C4	1555..1662	CDD:128421	18/87 (21%)
		C4	1663..1777	CDD:128421
COL5A3	NP_056534.2	LamG	31..210	CDD:304605
		Nonhelical region	211..391		21/84 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	230..304
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	322..362		9/41 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	387..439		24/51 (47%)
		Triple-helical region	392..1489		565/1429 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	476..1492		534/1349 (40%)
		Collagen	482..541	CDD:189968	27/65 (42%)
		Collagen	533..591	CDD:189968	30/76 (39%)
		Collagen	572..631	CDD:189968	31/64 (48%)
		Collagen	725..783	CDD:189968	26/59 (44%)
		Collagen	791..850	CDD:189968	30/73 (41%)
		Collagen	995..1053	CDD:189968	27/68 (40%)
		Collagen	1142..1201	CDD:189968	27/58 (47%)
		COLFI	1515..1743	CDD:279718	14/66 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910