DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col2a1b

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_001268407.1	Gene:	col2a1b / 503730	ZFINID:	ZDB-GENE-050302-9	Length:	1493	Species:	Danio rerio

Alignment Length:	1762	Identity:	619/1762 - (35%)
Similarity:	745/1762 - (42%)	Gaps:	481/1762 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 LLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRN----EP-KFPIDDSYDIV-------DS 60
            ||..|.:...|.|..||. :.....|..||...:.:::    || :..:.||..::       :.
Zfish    14 LLCVAALGALLTGGAAQD-EEQDPGGCSQDGQLYRDKDVWKPEPCRICVCDSGTVLCDEIVCEEL 77

  Fly    61 AGVARGDLPPKNCTAGYAGCVPKCIAEKGN--RGLPGPLGPTGLKGEM-GFPGMEGPSGDKGQKG 122
            ...|:.::|       ...|.|.|.:...:  ..|||..|..|..|:: ...|..||||..|..|
Zfish    78 RDCAKPEIP-------LGECCPVCASADASTPERLPGAKGQKGEPGDITDVVGPRGPSGPMGPPG 135

  Fly   123 DPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQ---------DGIPGLE 178
            :.||.|.||:|||:||||..|:.|.||..|..|.||.||.||..|..|.         |...|..
Zfish   136 EQGPRGDRGEKGEKGSPGPRGRDGEPGTPGNPGPPGPPGPNGPPGLGGNFASQMAGGFDDKAGAA 200

  Fly   179 GLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGF---PGEKGERGD 240
            .:..|.||.|..|..|..|..|.|                        |||||   |||.||.|.
Zfish   201 QMGVMQGPMGPMGPRGPPGPNGAP------------------------GPQGFQGNPGEGGEPGS 241

  Fly   241 SGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGE 305
            |||.|.:||.|..|..|:.|       :||.||..||:|.|             ||:|..|..|.
Zfish   242 SGPMGPRGPPGPPGKPGDDG-------EPGKPGNGGERGPP-------------GPQGARGFPGT 286

  Fly   306 PGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQK---GDRGEPGLNGLPG 367
            |||.|.||.   .|.|||||.|||.|..|..|:.|..|..|.||..|.:   |:||.||.:|..|
Zfish   287 PGLPGIKGH---RGYTGLDGAKGESGATGAKGESGSPGESGAPGPMGPRGLPGERGRPGPSGASG 348

  Fly   368 NPGQKGEPGRAGATGKPGLLGPPGPPG--GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQ 430
            ..|..|.||.||..|..|..|.||.||  |.:|..||.|.:||.   ||.||:|.:||.|..||.
Zfish   349 ARGNDGLPGGAGPPGPVGTAGSPGFPGSPGAKGEAGPTGARGPE---GAQGPRGESGVPGASGPS 410

  Fly   431 GYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGY--- 492
            |.:|..|..|:||..|:.|.|   |..|..|..||:|..||.|..||.||:||.||:||.|:
Zfish   411 GVSGNPGSDGMPGAKGSVGAP---GIGGAPGFPGPRGPPGPQGATGPLGPKGQSGDSGLAGFKGE 472

  Fly   493 -GIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQ 556
             |.:|..|:||:.|.||..|.:|:||.:|.              |||||.||..|:.|.||..|.
Zfish   473 AGPKGEIGNAGLQGAPGPAGEEGKRGPRGE--------------PGAAGPPGPTGERGTPGNRGF 523

  Fly   557 KGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPG 621
            .|..|:            |||||..|..|..|:.|..||.|.||.   |||.|..|..|.||.||
Zfish   524 PGQDGL------------AGPKGAPGERGPAGVSGPKGAGGDPGR---PGEPGLPGARGLTGRPG 573

  Fly   622 EKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEF 686
            :.|..|:.|..||.||.|:                  ||.||.:||:|..|..|.||..|..||.
Zfish   574 DAGPQGKVGPSGAPGEDGR------------------PGPPGPQGVRGQPGVMGFPGPKGGNGEA 620

  Fly   687 GFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSC 751
            |..|||||:||||..|.||:.|..|..|.||.:                 ||.|..||:|:||
Zfish   621 GKAGEKGLAGAPGLRGLPGKDGETGAAGPPGPA-----------------GSAGERGEQGQPG-- 666

  Fly   752 ALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKG 816
                    ..|.:|.||.   ||||||.|.||:            ||.||..|..|..|||||:|
Zfish   667 --------PSGFQGLPGP---PGPPGEGGKPGD------------QGVPGEAGGAGATGPRGERG 708

  Fly   817 NQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPG-ISRPGPMGPPGLNGLQGEKGDRGPTGPIGFP 880
            ..|..|..|..|..|.||:||..|..||:|..| ....|..|||||.|:.||:|..|..||
Zfish   709 FPGERGGAGPQGLQGPRGLPGTPGTDGPKGGVGPAGTAGAQGPPGLQGMPGERGTSGNPGP---- 769

  Fly   881 GADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGS 945
                    .|||||.|..|..|.||..|.:|..|||||.|.|||      :|.:|..|..|..|.
Zfish   770 --------KGDRGDNGDKGPEGAPGKDGSRGLTGPIGPTGPAGP------NGEKGESGPAGPSGV 820

  Fly   946 PGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRG 1010
            .|..|:||::|:.|.|   ||.||||..||.|:      |||.|.:|:.|..|..|..||.|..|
Zfish   821 AGTRGVPGDRGETGPP---GPAGFAGPPGADGQ------PGVKGEQGEGGQKGDAGAPGPQGPSG 876

  Fly  1011 PPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLR 1075
            .||..|..|:.|.:|..||.|..|..|.||..|..|.|   ||.|.||.|...|..|:.||.|:|
Zfish   877 APGPQGPTGVSGPKGARGAQGPPGATGFPGAAGRVGPP---GPNGNPGPAGPAGPPGKDGPKGVR 938

  Fly  1076 GDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEK 1140
            ||.||   ||.||:.||     .|.|||.|||||.|..|..|.||..|.:|..|.:|:.|.||::
Zfish   939 GDGGP---PGRPGDAGL-----RGSAGPAGEKGDPGEDGPHGPDGPAGPQGLAGQRGIVGLPGQR 995

  Fly  1141 GSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGP 1205
            |..|.|         ||||.:|.||..|.||..||:|.|            |||      |||
Zfish   996 GERGFP---------GLPGPSGEPGKQGAPGGPGDRGPP------------GPV------GAP-- 1031

  Fly  1206 KGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGAS 1270
                                                     ||||.||..|.:|:.|..||||..
Zfish  1032 -----------------------------------------GLTGAAGEPGREGNPGSDGPPGRD 1055

  Fly  1271 GLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLV 1335
            |..||.|.:||.||                             .|||            |.||..
Zfish  1056 GSAGIKGDRGDTGP-----------------------------AGAP------------GAPGGP 1079

  Fly  1336 GLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGL 1400
            |.|||:||.|.:|:||.||..|..|..|..||.|:.|..||:|.|||.|.:|..||||.:|..||
Zfish  1080 GAPGPVGPTGKQGDRGEAGPHGPSGPPGPAGARGMPGPQGPRGDKGEGGDSGDRGQKGHRGFTGL 1144

  Fly  1401 QGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPG 1465
            |   ||||..||.||      .|..||.|..|.||..||.|..|:||..||||..|      |||
Zfish  1145 Q---GLPGSPGQPGD------QGASGPSGPGGARGPPGPVGPAGKDGANGLPGPIG------PPG 1194

  Fly  1466 PKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPG--ERG 1528
            |:|..|:.|.:||.|.||.||.                           |||.|    ||  ...
Zfish  1195 PRGRSGETGPSGPPGTPGPPGP---------------------------PGPPG----PGIDMSA 1228

  Fly  1529 YEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAH 1593
            :.|...|        ..:|..|.|:      |..|:                     .||| :.|
Zfish  1229 FAGLSQL--------EKSPDPLRYM------RADQA---------------------ADGN-HQH 1257

  Fly  1594 NQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKT----------------------FWLTTN 1636
            :.::.         :||..|    ||......|.|.|                      :|:..|
Zfish  1258 DAEVD---------ATLKSL----NNQMENIRRPDGTKKSPARTCRDLKQCHPDWKSGEYWIDPN 1309

  Fly  1637 AAIPMMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGW-------EGLWIGYSFLMH 1694
            ....:..::           |.|........::.:...:|. .|.|       :.:|.|.:.
Zfish  1310 QGCTVDAIK-----------VFCNMETGESCIYPKPANIPR-KNWWTTKGGDRKHIWFGEAM--- 1359

  Fly  1695 TAVGNGG 1701
                |||
Zfish  1360 ----NGG 1362

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	28/57 (49%)
		Collagen	322..380	CDD:189968	28/60 (47%)
		Collagen	413..465	CDD:189968	22/51 (43%)
		Collagen	499..561	CDD:189968	24/61 (39%)
		Collagen	574..632	CDD:189968	27/57 (47%)
		Collagen	657..714	CDD:189968	29/56 (52%)
		Collagen	765..824	CDD:189968	26/58 (45%)
		Collagen	854..911	CDD:189968	24/56 (43%)
		Collagen	884..943	CDD:189968	25/58 (43%)
		Collagen	923..982	CDD:189968	23/58 (40%)
		Collagen	1028..1085	CDD:189968	26/56 (46%)
		Collagen	1229..1287	CDD:189968	21/57 (37%)
		Collagen	1318..1376	CDD:189968	22/57 (39%)
		Collagen	1399..1458	CDD:189968	29/58 (50%)
		Collagen	1477..1534	CDD:189968	14/58 (24%)
		C4	1555..1662	CDD:128421	18/128 (14%)
		C4	1663..1777	CDD:128421	8/46 (17%)
col2a1b	NP_001268407.1	VWC	39..94	CDD:278520	10/61 (16%)
		Collagen	264..322	CDD:189968	31/73 (42%)
		Collagen	315..389	CDD:189968	32/73 (44%)
		Collagen	368..424	CDD:189968	27/58 (47%)
		Collagen	468..526	CDD:189968	26/71 (37%)
		Collagen	498..557	CDD:189968	30/87 (34%)
		Collagen	537..585	CDD:189968	22/50 (44%)
		Collagen	711..769	CDD:189968	25/57 (44%)
		Collagen	744..803	CDD:189968	31/70 (44%)
		Collagen	795..852	CDD:189968	30/71 (42%)
		Collagen	828..899	CDD:189968	34/79 (43%)
		Collagen	954..1012	CDD:189968	30/66 (45%)
		COLFI	1260..1492	CDD:279718	20/135 (15%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910