DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col2a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_989220.1	Gene:	col2a1 / 394828	XenbaseID:	XB-GENE-6258353	Length:	1492	Species:	Xenopus tropicalis

Alignment Length:	1736	Identity:	616/1736 - (35%)
Similarity:	747/1736 - (43%)	Gaps:	412/1736 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 LLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRNEPKFPIDDSYDIVDSAGVARGDL---P 69
            :|:||.....|.....|..:.....||.....:.|:..:...|......:.|:..|...::   .
Frog    11 VLFAATQVILLAVVRCQDEEDVLATGSCVQHGQRYSDKDVWKPEPCQICVCDTGNVLCDEIICED 75

  Fly    70 PKNCTAG---YAGCVPKCIAE-----------KGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQ 120
            ||:|...   :..|.|.|..|           ||.:|.||.:  ..:.|..|.||.:||||::|.
Frog    76 PKDCPNAEIPFGECCPICPTEQSSTSSGQGVLKGQKGEPGDI--KDVVGPKGPPGPQGPSGEQGP 138

  Fly   121 KGDPGPYGQRGDKGERGSP---GLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSG 182
            :||      ||||||:|:|   |..|:.|.||..||.|.||.|            |.|||.|
Frog   139 RGD------RGDKGEKGAPGPRGRDGEPGTPGNPGPVGPPGPP------------GPPGLGG--- 182

  Fly   183 MPGPRGYAGQL-GSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGA 246
                 .:|.|: |...||.                    |.|.:...||..|..|.||..||.||
Frog   183 -----NFAAQMTGGFDEKA--------------------GGAQMGVMQGPMGPMGPRGPPGPTGA 222

  Fly   247 KGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGR 311
            .||:|..|..||          ||.||..|.                |||||..|..|:||..|.
Frog   223 PGPQGFQGNPGE----------PGEPGAGGP----------------MGPRGPPGPAGKPGDDGE 261

  Fly   312 KGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPG 376
            .|:||..|:.|..|.:|.:|.|            |.||..|.||.||.|||:|..|..|..|..|
Frog   262 AGKPGKSGERGPPGPQGARGFP------------GTPGLPGVKGHRGYPGLDGSKGEAGAAGAKG 314

  Fly   377 RAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGL 441
            ..||||:.|..||.||    ||.|      |.||..||.|..|..|.||||||.|..|..|.||.
Frog   315 EGGATGEAGSPGPMGP----RGLP------GERGRPGASGAAGARGNDGLPGPAGPPGPVGPAGA 369

  Fly   442 PGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGY-GIQGSKGDAGIPG 505
            ||.|   |.||.|||.|..|..||:|:.||.|..|.||..|..|.:|.||. ||.|:||.:|.||
Frog   370 PGFP---GAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPAGASGNPGTDGIPGAKGSSGAPG 431

  Fly   506 YPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKC 570
            ..|..|..|.|              |.||..||.|..|.||..|.||..|.||:.|.||::|...
Frog   432 IAGAPGFPGPR--------------GPPGPQGATGPLGPKGQTGDPGVAGFKGEHGPKGEIGSAG 482

  Fly   571 SSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGER---GHDGINGQTGPPGEKGEDGRTGLP 632
            .....||.|::|..|..|.||..|..|||||||.||.|   |.||:.|..|.|||:|..|..|..
Frog   483 PQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPK 547

  Fly   633 GATGEPGKPALCDLSLIEP-LKGDKGYPGAPGAKGVQ------GFKGAEGLPGIPGPKGEFGFKG 690
            ||.|:||:|.       || |.|.:|..|.||..|.|      |..|.:|.||.|||:|..|..|
Frog   548 GANGDPGRPG-------EPGLPGARGLTGRPGDAGPQGKVGPSGASGEDGRPGPPGPQGARGQPG 605

  Fly   691 EKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDE 755
            ..|..|..|.:|.||:||..|..|.||  ::|.||..|..||:|..|..|.:||:||
Frog   606 VMGFPGPKGANGEPGKAGEKGLLGAPG--LRGLPGKDGETGAQGPNGPAGPAGERGE-------- 660

  Fly   756 IKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGA 820
                    :|.||.:|..|.||..|||||      .|..|.||.||..|..||.|||||:|..|.
Frog   661 --------QGPPGPSGFQGLPGPPGSPGE------GGKPGDQGVPGEAGAPGLVGPRGERGFPGE 711

  Fly   821 VGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRP-GPMGPPGLNGLQGEKGDRGPTGPIGFPGADG 884
            .|..|..|..|.||:||..|..||:|..|.|.| |..|||||.|:.||:|..|.:||
Frog   712 RGSSGPQGLQGPRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGP-------- 768

  Fly   885 SVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLV 949
                .|||||.|..|..|.||..|.:|..|||||.|.:||
Frog   769 ----KGDRGDTGEKGPEGAPGKDGSRGLTGPIGPPGPSGP------------------------- 804

  Fly   950 GMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGA 1014
                 .|::|..|..||.|..|..||||.||..|.|                  ||.|..|||||
Frog   805 -----NGEKGESGPSGPAGIVGARGAPGDRGETGPP------------------GPAGFAGPPGA 846

  Fly  1015 PGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTG 1079
            .|..|:|||||.:            |:||:.|.||..||.|.||   .:|..|..||.|.||..|
Frog   847 DGQAGLKGDQGES------------GQKGDAGAPGPQGPSGAPG---PQGPTGVNGPKGARGAQG 896

  Fly  1080 PAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVG 1144
            |      ||..|.||.|  ||.||||..|:.|.||..|..|..|.||.:|..|..|:.|:.|..|
Frog   897 P------PGATGFPGAA--GRVGPPGPNGNPGPSGAPGSAGKEGPKGARGDAGPTGRAGDPGLQG 953

  Fly  1145 APGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGER 1209
            ..|:||..|..|..|.:|..|.   ||.:|..|:.|:.||||.:||      :||.|.|||.||.
Frog   954 PAGVPGEKGESGEDGPSGPDGP---PGPQGLSGQRGIVGLPGQRGE------RGFPGLPGPSGEP 1009

  Fly  1210 GIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNG 1274
            |.:|.|             ||.|:|   |..|..|..|||||||..|.:|:.|..||||..|..|
Frog  1010 GKQGGP-------------GSAGDR---GPPGPVGPPGLTGPAGEPGREGNAGSDGPPGRDGATG 1058

  Fly  1275 IPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPG 1339
            |.|.:|:.||                             :|||            |.||..|.||
Frog  1059 IKGDRGETGP-----------------------------LGAP------------GAPGAPGAPG 1082

  Fly  1340 PIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPS 1404
            |:||.|.:|:||.:|..|..|..|..||.||.|..||:|.|||.|..|..||||.:|..||||..
Frog  1083 PVGPTGKQGDRGESGPQGPLGPSGPAGARGLPGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLP 1147

  Fly  1405 GLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGE 1469
            |.||..|.:|.:         ||.|..|.||..||.|..|:||:.||||..|      ||||:|.
Frog  1148 GPPGTAGDQGAS---------GPAGPGGPRGPPGPVGPSGKDGSNGLPGPIG------PPGPRGR 1197

  Fly  1470 PGQPGRNGPKGEPGRPGERGLIG-----------IQGERGEKGERGLIGETGNVGRP-------- 1515
            .|:.|..||.|:||.||..|..|           .|.|:|....|.:..:..:...|
Frog  1198 GGETGPAGPPGQPGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVPQRDVDVEA 1262

  Fly  1516 -------------GPKGDRGEPGERGYEGAIGLIGQKGEPGAPAPAALDY-------LTGILITR 1560
                         .|.|.:..|.....:  :.|...:.:.|       ||       .|...|..
Frog  1263 TLKSLNNQIESIRSPDGTKKNPARTCRD--LKLCHPEWKSG-------DYWIDPNQGCTVDAIKV 1318

  Fly  1561 HSQSETVPAC--------------SAGHTE---LWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFS 1608
            ....||...|              ||...|   :|.|.::  ..|..:::..|..:|.:...:.:
Frog  1319 FCNMETGETCVYPNPSKIPKKNWWSAKGKEKKHIWFGETI--NGGFQFSYGDDSSAPNTANIQMT 1381

  Fly  1609 TLPVLSCGQNNVCNYASRNDKTFWLTTNA----AIPMMPVENIEIR 1650
            .|.:||........|..:|...|....:.    |:.:....::|||
Frog  1382 FLRLLSTDATQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIR 1427

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	29/59 (49%)
		Collagen	322..380	CDD:189968	21/57 (37%)
		Collagen	413..465	CDD:189968	27/51 (53%)
		Collagen	499..561	CDD:189968	23/61 (38%)
		Collagen	574..632	CDD:189968	30/60 (50%)
		Collagen	657..714	CDD:189968	26/62 (42%)
		Collagen	765..824	CDD:189968	29/58 (50%)
		Collagen	854..911	CDD:189968	24/56 (43%)
		Collagen	884..943	CDD:189968	20/58 (34%)
		Collagen	923..982	CDD:189968	15/58 (26%)
		Collagen	1028..1085	CDD:189968	20/56 (36%)
		Collagen	1229..1287	CDD:189968	28/57 (49%)
		Collagen	1318..1376	CDD:189968	22/57 (39%)
		Collagen	1399..1458	CDD:189968	26/58 (45%)
		Collagen	1477..1534	CDD:189968	17/88 (19%)
		C4	1555..1662	CDD:128421	22/117 (19%)
		C4	1663..1777	CDD:128421
col2a1	NP_989220.1	VWC	38..93	CDD:214564	9/54 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	98..1255		567/1464 (39%)
		Triple-helical region	206..1219		514/1272 (40%)
		Collagen	263..>303	CDD:189968	20/51 (39%)
		Med15	393..>785	CDD:312941	188/448 (42%)
		Collagen	497..556	CDD:189968	31/58 (53%)
		Collagen	807..865	CDD:189968	32/87 (37%)
		PRK07764	<816..1057	CDD:236090	125/306 (41%)
		Collagen	842..900	CDD:189968	33/78 (42%)
		Collagen	1094..1151	CDD:189968	28/56 (50%)
		Nonhelical region (C-terminal)	1220..1246		5/25 (20%)
		COLFI	1256..1491	CDD:366624	30/183 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910