Protein Alignment Col4a1 and Col4a2

DIOPT Version :10

• Sequence 1: NP_723044.1
• Gene: Col4a1 / 33727
• FlyBaseID: FBgn0000299
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_001414185.1
• Gene: Col4a2 / 306628
• RGDID: 1308085
• Length: 1707
• Species: Rattus norvegicus
• Alignment Length: 1866
• Identity: 799/1866 - (42%)
• Similarity: 970/1866 - (51%)
• Gaps: 343/1866 - (18%)

  Fly    62 GVARGDLP--PKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGE---MGFPGMEGPSGDKGQK 121
            ||.:.|:|  .::|:   .||  :|..|||.||.||.:||.|..|.   .||||::|..||||::
  Rat    34 GVKKSDVPCGGRDCS---GGC--QCFPEKGARGQPGEVGPQGYNGPPGLQGFPGLQGRKGDKGER 93

  Fly   122 GDPGPYGQRGDKGER---GSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGM 183
            |.|||.|.:||.|.|   |.||..|..|.||..||.|.||..|.||..|..|..|..|..|..|:
  Rat    94 GAPGPTGPKGDVGARGVSGFPGADGIPGHPGQGGPRGRPGYDGCNGTRGDAGPQGPSGTGGFPGL 158

  Fly   184 PGPRGYAGQLGSKGEKGEP---AKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYG 245
            |||:      |.||:||||   :||:.|..:||.|||   |:.|..||.|.||..|:.|..|..|
  Rat   159 PGPQ------GPKGQKGEPYALSKEDRDKYRGEPGEP---GSVGYQGPPGRPGPIGQMGPMGAPG 214

  Fly   246 AKGPRGEHGLKGE---KGASCYGPMKPGAPGIKGEKGEPASSFP--VKPTHTVMGP--------- 296
            ..||.|..|.||:   :|...||.        |||||:.....|  :....|::||
  Rat   215 RPGPPGPPGPKGQPGNRGLGFYGE--------KGEKGDVGQPGPNGIPSDITLIGPTPSTYHPDM 271

  Fly   297 -RGDMGQKGEPGLVG--RKGE------------PGPEGDTGLDGQKGEKGL-----PGGP-GDRG 340
             :|:.|.:||||:.|  .|||            ||.:|:.|:.||||.:||     |.|| |.:|
  Rat   272 YKGEKGSQGEPGIPGITLKGEEGIMGFPGTRGFPGLDGEKGVSGQKGSRGLDGFQGPSGPRGPKG 336

  Fly   341 RQGNFGPPGSTG-------QKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRG 398
            .:|..||||...       .||.||:||..|..|.||.:|||      |.||.:||||...|...
  Rat   337 ERGELGPPGPPAYSPHPSLAKGARGDPGFQGAHGEPGSRGEP------GDPGPVGPPGLSIGDED 395

  Fly   399 TP-GPPGPKGPRGYVGAPGPQ----GLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKG 458
            :. |.||..||:|:.|.|||.    |..|.||.|||||.            ||..||||..|
  Rat   396 SKRGLPGEMGPKGFSGEPGPSAYYPGPPGADGKPGPQGL------------PGPAGPPGPDG--- 445

  Fly   459 TAGLNGPKGSIGPIGHPGP---PGPEGQKGDAGLPGYGIQGSKGDAG-------IPGYPGLKGSK 513
              .|.|.|||.|.:|:|||   ||..|||           |.||:||       |||.|||.|.|
  Rat   446 --FLFGLKGSEGRVGYPGPSGFPGGRGQK-----------GWKGEAGDCQCGQVIPGLPGLPGPK 497

  Fly   514 GERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPK 578
            |..|..|..|..||.  |.||..|..|.||.||..|..|.||.|   |:|||      |.....|
  Rat   498 GFPGVNGEFGKKGDQ--GDPGLHGIPGFPGFKGAPGIAGAPGPK---GVKGD------SRTITTK 551

  Fly   579 GDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPAL 643
            |::|..|:||:      .|..|:.|.||..|.||..|..||||    ||..|.||..|.||.|
  Rat   552 GERGQPGIPGV------HGMKGDDGVPGRDGLDGFPGLPGPPG----DGIKGPPGDAGLPGTP-- 604

  Fly   644 CDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAG 708
                      |.||:||..|..|          .|:||||||.||.|:.||.|.||..|.|
  Rat   605 ----------GTKGFPGEVGPPG----------QGLPGPKGERGFPGDAGLPGPPGFPGPP---- 645

  Fly   709 RDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTG-M 772
              |.||.|||             |..|.|                  :|.|..|.:....|.| :
  Rat   646 --GLPGTPGQ-------------ADCDTG------------------VKRPIGGGQQVVIQPGCV 677

  Fly   773 PGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPG 837
            .||.|..|.||..|.||.||..|..|.||..|.:||.|..|:.|.:   |.||.||..|.||..|
  Rat   678 EGPAGSPGQPGPPGPTGAKGIRGIPGFPGASGEQGLKGFPGDPGRE---GFPGPPGFMGPRGSKG 739

  Fly   838 RNGQPGPRGEPG-ISRPGPMGPPGLNGLQGE--------KGDRGPTGPIGFPGADGSVGYPGDRG 893
            ..|.|||.|.|| |..|||.||||..|:.||        :||.|..|..|..|..|.:|.||.||
  Rat   740 APGLPGPDGPPGPIGLPGPAGPPGDRGIPGEVLGAQPGARGDAGLPGQPGLKGFPGEIGAPGFRG 804

  Fly   894 DAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDR 958
            ..|:||:   ||:.|:.|..||.|..|::||||..|..|..||:|..|.||||||.|:||::|:.
  Rat   805 SQGMPGM---PGLKGQPGFPGPSGQPGLSGPPGQHGFPGAPGREGPLGLPGSPGLGGLPGDRGEP 866

  Fly   959 GAPGNDGPKGFAGVT---------------GAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGG 1008
            |.||..||.|..||:               |:||.:|.||:||:.|.|||:|:.|:.|..|.:|.
  Rat   867 GEPGEPGPVGMKGVSGDRGDAGVSGERGHPGSPGFKGMAGMPGIPGQKGDRGSPGMDGFQGMLGL 931

  Fly  1009 RGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPG--LPGDASEKGQKGEPGP 1071
            :|.||.|   ||||:.|..|.||.:||.|.||.|||:|..|..|||.  |||....||:||:.||
  Rat   932 KGRPGFP---GIKGEAGFFGVPGLKGLPGEPGVKGNRGDRGPPGPPPLILPGMKDIKGEKGDEGP 993

  Fly  1072 SGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQ 1136
            .||:|..|..|..|.|   |:|||:                                |:.|:.|:
  Rat   994 MGLKGYLGLKGIQGMP---GVPGLS--------------------------------GIPGLPGR 1023

  Fly  1137 PG-EKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQG-- 1198
            || .||:.|..|:||.||:.|.||.:|.||..|:||..|.:||.|..|:.|:.|||||.|..|
  Rat  1024 PGFIKGAKGDIGVPGTPGLPGFPGVSGPPGITGFPGFTGSRGEKGTPGVAGVFGETGPTGDFGDI 1088

  Fly  1199 -----FTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAK 1258
                 ..|:||.|||||:.|.|||..    :.|:||::|:.|:.|..|..|.:|..|..|..|..
  Rat  1089 GDTVDLPGSPGLKGERGVTGIPGLKG----LFGEKGAEGDVGFPGITGMAGAQGSPGLKGQTGFP 1149

  Fly  1259 GDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVT-IKGEKGLPGRPGRNGRQGLIGAPGL--I 1320
            |..|||||.|..|..||||.|||.|..|..|.||:. |:|..||.|.|   |.:|..|:||:  .
  Rat  1150 GLTGLQGPQGEPGRIGIPGDKGDFGWPGVPGRPGIPGIRGISGLHGLP---GTKGFPGSPGVDAH 1211

  Fly  1321 GERGLPGLAGEPGLVG----LPGPIGPAGSKGERGLAGS---PGQPGQDGFP------------- 1365
            |:.|.||..|:.|..|    ||||:|..|.|||:|:.|.   .|.||..|||
  Rat  1212 GDPGFPGPTGDRGDRGEANTLPGPVGAPGQKGEQGIPGERGPVGSPGLQGFPGISPPSNISGLPG 1276

  Fly  1366 --GAPGL------KGDTGP------QGFKGERGLN---GFEGQKGDKGDRGLQGPSGLPGLVGQK 1413
              ||||:      :|..||      .|.||:.|.:   ||.|:||..||.|.||..|:.||.|:|
  Rat  1277 DVGAPGIFGLQGYQGPPGPPGPNALPGIKGDEGSSGAAGFPGEKGWVGDPGPQGQPGVHGLPGEK 1341

  Fly  1414 GDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPG-PKGEPGQPGRNG 1477
            |..|..|..||.||.||.|:|   ||||..|..|.||.||..|.||:   || |:....|||..|
  Rat  1342 GPKGEQGFMGNTGPSGAVGDR---GPKGPKGDQGFPGAPGSMGSPGI---PGIPQKIAVQPGTMG 1400

  Fly  1478 PKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPK---GDRGEPGERGYEGAIGLIGQK 1539
            |:|..|.||..|.:|.||..|:.|.||..|:.|..||.|..   |.||:.|..|::|.||..|:.
  Rat  1401 PQGRRGLPGALGEMGPQGPPGDPGFRGAPGKAGPQGRGGVSAVPGFRGDQGPMGHQGPIGQEGEP 1465

  Fly  1540 GEPGAPAPAAL---DYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPG 1601
            |.||:|....:   ....|.|:.:|||::..|.|..|..:||:||||||.:|.:.|||||||..|
  Rat  1466 GRPGSPGLPGMPGRSVSIGYLLVKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAG 1530

  Fly  1602 SCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEAPANVI 1666
            ||:.||||:|.|.|...:||.|||||||::||:|.|.:|||||...||:.|||||.||||||..|
  Rat  1531 SCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEEEIKPYISRCSVCEAPAVAI 1595

  Fly  1667 AVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGTC 1731
            |||||.:.:|.||.||..||||||||||||.|:.||||:|.|||||||||||||||||||.:|||
  Rat  1596 AVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTC 1660

  Fly  1732 HFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMKN 1777
            |::....|||:..:........|...|:|||..::|:|||||||||
  Rat  1661 HYFANKYSFWLTTIPEQNFQSTPSADTLKAGLIRTHISRCQVCMKN 1706

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	gly_rich_SclB	<107..>361	CDD:468478	120/301 (40%)
		gly_rich_SclB	<355..>642	CDD:468478	126/301 (42%)
		gly_rich_SclB	<543..820	CDD:468478	100/277 (36%)
		gly_rich_SclB	<727..>968	CDD:468478	104/250 (42%)
		gly_rich_SclB	<969..>1218	CDD:468478	110/273 (40%)
		gly_rich_SclB	<1186..>1420	CDD:468478	115/280 (41%)
		gly_rich_SclB	<1321..>1547	CDD:468478	112/266 (42%)
		C4	1555..1662	CDD:128421	62/106 (58%)
		C4	1663..1777	CDD:128421	69/113 (61%)
Col4a2	NP_001414185.1	None