DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col27a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_942042.1	Gene:	Col27a1 / 298101	RGDID:	735115	Length:	1855	Species:	Rattus norvegicus

Alignment Length:	1506	Identity:	543/1506 - (36%)
Similarity:	693/1506 - (46%)	Gaps:	363/1506 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   346 GPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRG 410
            |||||.|..|..|.|||.||||:||.:|..|..|..|.|||   |||| |.:|..|.||....:.
  Rat   619 GPPGSKGDCGLPGPPGLPGLPGSPGPRGPRGPPGPFGNPGL---PGPP-GAKGQKGDPGLSPGQA 679

  Fly   411 YVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHP 475
            :.||.|..||.|:.|.|||.|..|.||..|..|.||.:|.||.:|..|..|..|.:|..||:
  Rat   680 HDGAKGNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPV--- 741

  Fly   476 GPPGPEGQKGDAGLPG-YGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAA 539
            |.|||:|.:|..|||| :|:.||.|:.|:||.||.:|..|..||      |||  .|..|.||..
  Rat   742 GDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGEMGRPGF------PGD--FGERGPPGLD 798

  Fly   540 GAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGY 604
            |.||:.|..|.||..|..||||..|.|                                    ||
  Rat   799 GNPGEIGLPGPPGVLGLLGDMGALGPV------------------------------------GY 827

  Fly   605 PGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQG 669
            ||.:|..|:.|..|.||.||:.|..|:||.:|:||            .:||||..|.|      |
  Rat   828 PGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPG------------FQGDKGSHGLP------G 874

  Fly   670 FKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKG 734
            |.||.|.   |||.|:.|.||..||.|.||.:|.||..              |.||.:|.:|.
  Rat   875 FPGARGK---PGPMGKAGDKGSLGLPGPPGPEGFPGDI--------------GPPGDNGPEGM-- 920

  Fly   735 DKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGP 799
                                         ||:||..|:|||||:         .|.:|:.||.||
  Rat   921 -----------------------------KGKPGARGLPGPPGQ---------LGPEGDEGPMGP 947

  Fly   800 PGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGL 864
            |||.   ||.|..|.|      |.||.||.||.:|.||..|:|||.||.|:.  |.:|..|..|:
  Rat   948 PGVP---GLEGQPGRK------GFPGRPGLDGSKGEPGDPGRPGPVGEQGLM--GFVGLVGEPGI 1001

  Fly   865 QGEKGDR---------GPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAG 920
            .||||||         ||.|.:|.||..|.||.||:.|..|.||..|.||:.|.||..||.||
  Rat  1002 VGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGP-- 1064

  Fly   921 VAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGV---TGAPGKRGPA 982
             .||.|..|..|::||.|.:|.||.||..|..|.:|..||      |||.|:   :|.||.:|..
  Rat  1065 -DGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGA------KGFLGIPGPSGPPGAKGLP 1122

  Fly   983 GIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGF 1047
            |.||..|.:|..|..|..|..||.|..|.||.||..|:|||.|..|.||:|||.|..||.|.:|.
  Rat  1123 GEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGD 1187

  Fly  1048 PGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGR 1112
            .|..||.||      ||.:|:|||.|..|:.|..|.   .||:|||        ||||..|.:||
  Rat  1188 LGPVGPDGL------KGDRGDPGPDGEHGEKGQEGL---KGEEGLP--------GPPGITGVRGR 1235

  Fly  1113 SGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKG 1177
               :|:.|..||||::|.:|..|..|:.|.:|.||.||.||..|..|:.|..|.:|.||..|.:|
  Rat  1236 ---EGKPGSQGEKGQRGAKGAKGYQGQLGEMGIPGDPGPPGTPGPKGSRGTLGPMGAPGRMGAQG 1297

  Fly  1178 EPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGE 1242
            ||||:|..|.||.|||:      |.||||||:|.:|:.|.....|...|::|..|:|   |::||
  Rat  1298 EPGLAGYNGHKGITGPL------GPPGPKGEKGEQGEDGKTEGAPGPPGERGPVGDR---GDRGE 1353

  Fly  1243 QGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGR 1307
            .|:.|..|..||.|.:|:.|.||.||..|..|.||.||.   :||        :|.||.||:.|.
  Rat  1354 PGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGS---KGE--------EGPKGKPGKAGA 1407

  Fly  1308 NGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKG 1372
            :||:                  |..||.|||||.|..|.:|..|:||..|.||:||.|
  Rat  1408 SGRR------------------GTQGLQGLPGPRGVVGRQGPEGMAGQDGNPGRDGRP------- 1447

  Fly  1373 DTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGF- 1436
                          |::|::|:.||.|..||:      |::|:.|..||.|..||.|..||.|.
  Rat  1448 --------------GYQGEQGNDGDPGPVGPA------GRRGNPGVAGLPGAQGPPGFKGESGLP 1492

  Fly  1437 --TGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGE---PGQPGRNGPKGEPGRPGERGLIGIQGE 1496
              .||.|:.|.:|..||||.:||||....||..||   ||..|..||||.||..|.:|:.|.:|.
  Rat  1493 GQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGP 1557

  Fly  1497 RGEKGERGLIGETGNV---GRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAPA--ALDYLTGI 1556
            .|..|:.|:||..|.:   |.||||||||..|:.|.:|..|..|.:|.||.|.|.  .:.:
  Rat  1558 PGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDWGLQGPRGPPGPRGRPGPPGPPWHPVQF---- 1618

  Fly  1557 LITRHSQSETVPAC------SAGHTELWTGYS----LLYVDGND------YAHN--QDLGSP-GS 1602
                  |.:.:.|.      :.|...|..|||    |:...|.:      |..|  |.:.:| |:
  Rat  1619 ------QQDDLEAAFQTWMDAHGAVRLEQGYSYPDQLMLDQGGEIFKTLHYLSNLIQSIKTPLGT 1677

  Fly  1603 -------------CVPRFS-----TLPVLSCGQNNV---CNYASRNDKTFWLTTNAAIPMMPVEN 1646
                         |..:.:     ..|.|.|..:.:   ||:          |......:.|:..
  Rat  1678 KENPARVCRDLMDCEQKMADGIYWVDPNLGCSSDTIEVSCNF----------THGGQTCLKPITA 1732

  Fly  1647 IEIRQYISRC------VVCEAPANVIAVH--SQTI--EVPDCPNGWEGL----WIGYSFLMHTAV 1697
            .:....:||.      ::.......|.:|  :.|:  |.|..|:..:.:    |.|..|
  Rat  1733 SKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPARPSARQAVRFRAWNGQVF------ 1791

  Fly  1698 GNGGGGQALQSPGSCLEDFRATPFIECNGAK---GTCHFYETMTSFW--------MYNLESSQPF 1751
              ..|||           ||  |.:..:|.|   |..|  :|:.:|.        :.::::..|.
  Rat  1792 --EAGGQ-----------FR--PEVSMDGCKVHDGRWH--QTLFTFRTQDPQQLPIVSVDNLPPV 1839

  Fly  1752 ERPQQQTIKAG 1762
            ...:|..::.|
  Rat  1840 SSGKQYRLEVG 1850

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968
		Collagen	322..380	CDD:189968	19/33 (58%)
		Collagen	413..465	CDD:189968	24/51 (47%)
		Collagen	499..561	CDD:189968	27/61 (44%)
		Collagen	574..632	CDD:189968	14/57 (25%)
		Collagen	657..714	CDD:189968	23/56 (41%)
		Collagen	765..824	CDD:189968	24/58 (41%)
		Collagen	854..911	CDD:189968	29/65 (45%)
		Collagen	884..943	CDD:189968	28/58 (48%)
		Collagen	923..982	CDD:189968	26/61 (43%)
		Collagen	1028..1085	CDD:189968	26/56 (46%)
		Collagen	1229..1287	CDD:189968	24/57 (42%)
		Collagen	1318..1376	CDD:189968	20/57 (35%)
		Collagen	1399..1458	CDD:189968	25/61 (41%)
		Collagen	1477..1534	CDD:189968	28/59 (47%)
		C4	1555..1662	CDD:128421	24/152 (16%)
		C4	1663..1777	CDD:128421	24/119 (20%)
Col27a1	NP_942042.1	LamG	52..230	CDD:304605
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	317..428
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	511..580
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	617..787		84/180 (47%)
		Triple-helical region	619..1612		494/1223 (40%)
		Collagen	688..746	CDD:189968	27/60 (45%)
		Collagen	718..777	CDD:189968	29/61 (48%)
		Collagen	754..810	CDD:189968	29/63 (46%)
		Collagen	826..884	CDD:189968	31/78 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	838..1617		384/958 (40%)
		Collagen	913..984	CDD:189968	40/119 (34%)
		Collagen	1047..1105	CDD:189968	28/66 (42%)
		Collagen	1139..1193	CDD:189968	26/53 (49%)
		Collagen	1168..1227	CDD:189968	32/75 (43%)
		Collagen	1201..1259	CDD:189968	29/71 (41%)
		Collagen	1334..1391	CDD:189968	24/59 (41%)
		Collagen	1376..1433	CDD:189968	30/85 (35%)
		Collagen	1439..1498	CDD:189968	27/85 (32%)
		Collagen	1472..1529	CDD:189968	27/56 (48%)
		Collagen	1511..1570	CDD:189968	27/58 (47%)
		COLFI	1656..1854	CDD:295304	39/228 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910