DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and let-2

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_510663.1	Gene:	let-2 / 181708	WormBaseID:	WBGene00002280	Length:	1759	Species:	Caenorhabditis elegans

Alignment Length:	1758	Identity:	880/1758 - (50%)
Similarity:	1021/1758 - (58%)	Gaps:	97/1758 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly    79 GCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHG 143
            ||.  |:.|||:.|.|||.||.|.:|..||||.||.:|.||.||..||.|..|.||:||:.|:.|
Worm    37 GCF--CVGEKGSMGAPGPQGPPGTQGIRGFPGPEGLAGPKGLKGAQGPPGPVGIKGDRGAVGVPG 99

  Fly   144 QAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGD 208
            ..|..|..|..|.||.||..|.|||:|.||.||:.|..|.||..|:.|..|..|.|||||.....
Worm   100 FPGNDGGNGRPGEPGPPGAPGWDGCNGTDGAPGIPGRPGPPGMPGFPGPPGMDGLKGEPAIGYAG 164

  Fly   209 YAKGEKGEPGWRGTAGLAGP---QGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPM-KP 269
             |.||||:.|..|..||.||   .|:|||||:|||:|..|.:||.||.|..|..|....||. .|
Worm   165 -APGEKGDGGMPGMPGLPGPSGRDGYPGEKGDRGDTGNAGPRGPPGEAGSPGNPGIGSIGPKGDP 228

  Fly   270 GAPGIKGEKGEPASSFPVKPTHTVMGPRGDM---GQKGEPGLVGRKGEPGP---EGDTGLDGQKG 328
            |..|..|..|.|..........|::||:||:   |:|||||..|::|.||.   .|..||.|.||
Worm   229 GDIGAMGPAGPPGPIASTMSKGTIIGPKGDLGEKGEKGEPGEGGQRGYPGNGGLSGQPGLPGMKG 293

  Fly   329 EKGL--PGGP-GDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPP 390
            ||||  |.|| |..||.||.||||.   |||||..||.|:||.||||||.|..|..|..|..|||
Worm   294 EKGLSGPAGPRGKEGRPGNAGPPGF---KGDRGLDGLGGIPGLPGQKGEAGYPGRDGPKGNSGPP 355

  Fly   391 GPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKG 455
            ||||||....|.|||.|..|..|.|||.|.:|..|.|||.|..|..||.||||.|||||.||.||
Worm   356 GPPGGGTFNDGAPGPPGLPGRPGNPGPPGTDGYPGAPGPAGPIGNTGGPGLPGYPGNEGLPGPKG 420

  Fly   456 EKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKG 520
            :||..|:.|..|..||.|.||.|||:|:.|..|.||..|.|..|..|.||..|..|.|||.|..|
Worm   421 DKGDGGIPGAPGVSGPSGIPGLPGPKGEPGYRGTPGQSIPGLPGKDGKPGLDGAPGRKGENGLPG 485

  Fly   521 NAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSG 585
            ..|.||||..|.||.||..||||..|..||.|..|..|..|.|||.||.||:|..|.||:||..|
Worm   486 VRGPPGDSLNGLPGAPGQRGAPGPNGYDGRDGVNGLPGAPGTKGDRGGTCSACAPGTKGEKGLPG 550

  Fly   586 LPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIE 650
            ..|.||..|.||.||..|..|:.|.||:.|..|.||..|..|:.|.||..|:.|:|....|.
Worm   551 YSGQPGPQGDRGLPGMPGPVGDAGDDGLPGPAGRPGSPGPPGQDGFPGLPGQKGEPTQLTLR--- 612

  Fly   651 PLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEF------GFKGEKGLSGAPGNDGTPGRAGR 709
                 .|.||.||.||..||.|..|:.|:|||.|..      |:.||||.:|.||..|.||:.|.
Worm   613 -----PGPPGYPGLKGENGFPGQPGVDGLPGPSGPVGPPGAPGYPGEKGDAGLPGLSGKPGQDGL 672

  Fly   710 DGYPGIPGQSIKGEPGFHGRDGAKGD---KGSFGRSGEKGEPGSCALDEIKMPA-------KGNK 764
            .|.||..|::..|:||..|..|||||   .|..|..|.:|.||..|.:....||       .|..
Worm   673 PGLPGNKGEAGYGQPGQPGFPGAKGDGGLPGLPGTPGLQGMPGEPAPENQVNPAPPGQPGLPGLP 737

  Fly   765 GEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGK 829
            |..|:.|.||.|||.|.||..|..|:||::|..||||:.|..|:.|.:|..|..|..|:||..|.
Worm   738 GTKGEGGYPGRPGEVGQPGFPGLPGMKGDSGLPGPPGLPGHPGVPGDKGFGGVPGLPGIPGPKGD 802

  Fly   830 DGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGD--- 891
            .|..|:||.|||   :||||:..||..|.||..||   |||.|..|..|.||.:|..|:||.
Worm   803 VGNPGLPGLNGQ---KGEPGVGVPGQPGSPGFPGL---KGDAGLPGLPGTPGLEGQRGFPGAPGL 861

  Fly   892 RGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKG 956
            :|..||||:||:||..|||||.|..|..|..|.||.||.||:.|..|.|||.|.|||.|:.|.||
Worm   862 KGGDGLPGLSGQPGYPGEKGDAGLPGVPGREGSPGFPGQDGLPGVPGMKGEDGLPGLPGVTGLKG 926

  Fly   957 DRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIK 1021
            |.||||..|..|..|..|.||.:|.||||||.|.|||.|..||.|.:||.|..|.||.||..|:|
Worm   927 DLGAPGQSGAPGLPGAPGYPGMKGNAGIPGVPGFKGDGGLPGLPGLNGPKGEPGVPGMPGTPGMK 991

  Fly  1022 GDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGE---PGPSGLRGDTGPAGT 1083
            |:.||.|.||:.||.|:||.||::||.||.|..|..|.|:..||||:   ||..||||..||:|.
Worm   992 GNGGLPGLPGRDGLSGVPGMKGDRGFNGLPGEKGEAGPAARDGQKGDAGLPGQPGLRGPQGPSGL 1056

  Fly  1084 PGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGI 1148
            ||.||.||..||..:|:.|.|||||..|..|..||.|..|..|:.||.|.   ||.||..|.||.
Worm  1057 PGVPGFKGETGLPGYGQPGQPGEKGLPGIPGKAGRQGAPGSPGQDGLPGF---PGMKGESGYPGQ 1118

  Fly  1149 PGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLS---GLPGLKGETGPVGL------QGFTGAPG 1204
            .|.||.|||||..|..|.:|..|..|..|.|||.   |:||::|:.|..||      :|..|.||
Worm  1119 DGLPGRDGLPGVPGQKGDLGQSGQPGLSGAPGLDGQPGVPGIRGDKGQGGLPGIPGDRGMDGYPG 1183

  Fly  1205 PKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGA 1269
            .|||.|..||||||.    :.|:||..|..|:.|.||..|..|..|..|:.|.|||.|..|.||.
Worm  1184 QKGENGYPGQPGLPG----LGGEKGFAGTPGFPGLKGSPGYPGQDGLPGIPGLKGDSGFPGQPGQ 1244

  Fly  1270 SGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGL 1334
            .||.|:.|.||..|..|..|.||.:|.|..|.||.||..|:.|..|.||..||.||.||.|.|||
Worm  1245 EGLPGLSGEKGMGGLPGMPGQPGQSIAGPVGPPGAPGLQGKDGFPGLPGQKGESGLSGLPGAPGL 1309

  Fly  1335 VGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRG 1399
            .|..|..|..|:||:.|..|.||:.|:||.||.||..|..|..|.|||.|..|..||.|..|..|
Worm  1310 KGESGMPGFPGAKGDLGANGIPGKRGEDGLPGVPGRDGQPGIPGLKGEVGGAGLPGQPGFPGIPG 1374

  Fly  1400 LQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGM---L 1461
            |:|..||||..|.||:.|:|         |.||..|:.|.|| ||  |.|||||:.|.||.   :
Worm  1375 LKGEGGLPGFPGAKGEAGFP---------GTPGVPGYAGEKG-DG--GLPGLPGRDGLPGADGPV 1427

  Fly  1462 PPPGPKG-----EPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDR 1521
            .||||.|     |||:.|..|..|.||..||:|:.|:.|..|..|..||.|:.||.|.||..|..
Worm  1428 GPPGPSGPQNLVEPGEKGLPGLPGAPGLRGEKGMPGLDGPPGNDGPPGLPGQRGNDGYPGAPGLS 1492

  Fly  1522 GEPGERGYEGAIGLIGQKGEPGAP----APAALD--YLTGILITRHSQSETVPACSAGHTELWTG 1580
            ||.|..|..|..||.||.|.||||    ||.|..  |..|.::.:|||:..||.|..|.|:||.|
Worm  1493 GEKGMGGLPGFPGLDGQPGGPGAPGLPGAPGAAGPAYRDGFVLVKHSQTTEVPRCPEGQTKLWDG 1557

  Fly  1581 YSLLYVDGNDYAHNQDLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVE 1645
            |||||::||:.:||||||..|||:.||||:|.|.|..||||||||||||::||:|:.|||||||.
Worm  1558 YSLLYIEGNEKSHNQDLGHAGSCLQRFSTMPFLFCDFNNVCNYASRNDKSYWLSTSEAIPMMPVN 1622

  Fly  1646 NIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPG 1710
            ..||..|||||.|||||||.||||||||::|:||.||..|||||||.|||..|..||||:|.|||
Worm  1623 EREIEPYISRCAVCEAPANTIAVHSQTIQIPNCPAGWSSLWIGYSFAMHTGAGAEGGGQSLSSPG 1687

  Fly  1711 SCLEDFRATPFIECNGAKGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCM 1775
            |||||||||||||||||:|:||::....|||:..:::...|:.|:.||:|:|..::.|||||||:
Worm  1688 SCLEDFRATPFIECNGARGSCHYFANKFSFWLTTIDNDSEFKVPESQTLKSGNLRTRVSRCQVCV 1752

  Fly  1776 KNS 1778
            |::
Worm  1753 KST 1755

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	28/56 (50%)
		Collagen	322..380	CDD:189968	38/60 (63%)
		Collagen	413..465	CDD:189968	30/51 (59%)
		Collagen	499..561	CDD:189968	31/61 (51%)
		Collagen	574..632	CDD:189968	26/57 (46%)
		Collagen	657..714	CDD:189968	29/62 (47%)
		Collagen	765..824	CDD:189968	27/58 (47%)
		Collagen	854..911	CDD:189968	29/59 (49%)
		Collagen	884..943	CDD:189968	32/61 (52%)
		Collagen	923..982	CDD:189968	32/58 (55%)
		Collagen	1028..1085	CDD:189968	31/59 (53%)
		Collagen	1229..1287	CDD:189968	25/57 (44%)
		Collagen	1318..1376	CDD:189968	29/57 (51%)
		Collagen	1399..1458	CDD:189968	28/58 (48%)
		Collagen	1477..1534	CDD:189968	25/56 (45%)
		C4	1555..1662	CDD:128421	67/106 (63%)
		C4	1663..1777	CDD:128421	70/113 (62%)
let-2	NP_510663.1	Collagen	181..240	CDD:189968	27/58 (47%)
		Collagen	429..490	CDD:189968	29/60 (48%)
		Collagen	470..531	CDD:189968	31/60 (52%)
		Collagen	791..844	CDD:189968	28/58 (48%)
		Collagen	821..879	CDD:189968	28/60 (47%)
		Collagen	899..958	CDD:189968	33/58 (57%)
		Collagen	1004..1061	CDD:189968	30/56 (54%)
		Collagen	1072..1131	CDD:189968	32/61 (52%)
		Collagen	1117..1176	CDD:189968	25/58 (43%)
		Collagen	1202..1253	CDD:189968	22/50 (44%)
		Collagen	1308..1367	CDD:189968	28/58 (48%)
		Collagen	1347..1405	CDD:189968	28/66 (42%)
		C4	1533..1637	CDD:279721	64/103 (62%)
		C4	1641..1751	CDD:279721	67/109 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	161	1.000	Domainoid score	I2450
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1390
Inparanoid	1	1.050	1570	1.000	Inparanoid score	I39
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2460
SonicParanoid	1	1.000	-	-		X1239
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	10.900