DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and COL5A1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_000084.3	Gene:	COL5A1 / 1289	HGNCID:	2209	Length:	1838	Species:	Homo sapiens

Alignment Length:	1703	Identity:	630/1703 - (36%)
Similarity:	738/1703 - (43%)	Gaps:	458/1703 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 KTAGTAGSIQD-SVKHYNRNEPKFPIDDSY--DIVDSAGVARGDLPPKNCTAGYAGC-VPKCIAE 87
            :|:..||..:| .:..|:    ..|.:|.|  ...|......|:..|...|...||. :|...|:
Human   322 ETSEGAGKEEDVGIGDYD----YVPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTAD 382

  Fly    88 KGNRGLPGP-----------------------------LGPTGLKGEMG----------FPGMEG 113
            ..|...|.|                             ..||....|:|          :.|:.|
Human   383 TSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGG 447

  Fly   114 PSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLE 178
            |.|:|||||:|...          .||:..: |.||.:||||.||.||..|.
Human   448 PRGEKGQKGEPAII----------EPGMLIE-GPPGPEGPAGLPGPPGTMGP------------- 488

  Fly   179 GLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQG----FPGEKGERG 239
                       .||:|..||:|.|            |.||..|..||.||.|    .|...|..|
Human   489 -----------TGQVGDPGERGPP------------GRPGLPGADGLPGPPGTMLMLPFRFGGGG 530

  Fly   240 DSGPYG--------------------AKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASS 284
            |:|..|                    .:||.|..||.|..|.  .||  ||:.|:|||.|:
Human   531 DAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGP--VGP--PGSGGLKGEPGD---- 587

  Fly   285 FPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPG 349
                     :||:|..|.:|.||..|:   ||..|..|.||.:|..|..|..||||..|..|.|
Human   588 ---------VGPQGPRGVQGPPGPAGK---PGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLP- 639

  Fly   350 STGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPG--GGRGTPGPPGPKGPRGYV 412
              |:||.||:||.:|.||.||..||.|..|..|..||.|.|||.|  |.:|.||||||.|..|..
Human   640 --GEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMD 702

  Fly   413 GAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGP 477
            |.|||:|..|..|.|||.|..|..|..||||..|..||||:||..|..||.|..|:.||.||||.
Human   703 GQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGK 767

  Fly   478 PGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAP 542
            .||.|:||..|.|     |.:|..|.||..|:||:.|.||.||.
Human   768 EGPPGEKGGQGPP-----GPQGPIGYPGPRGVKGADGIRGLKGT--------------------- 806

  Fly   543 GQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGE 607
              ||:.|..|.||.||||||               |||:|..|.||..|:||..||.| ||
Human   807 --KGEKGEDGFPGFKGDMGI---------------KGDRGEIGPPGPRGEDGPEGPKG-RG---- 849

  Fly   608 RGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKG 672
             |.:|..|..|||||||:.|..||||..|..|.            ||..|:||.|||.|.:|.:|
Human   850 -GPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGP------------KGSIGFPGFPGANGEKGGRG 901

  Fly   673 AEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQ-SIKGEPGFHGRDGAKGDK 736
            ..|.||..|.:|..|.:||:|..|..|..|..|.:|.||..|.||: ...|..|..|..|.||..
Human   902 TPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPP 966

  Fly   737 GSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPP---GEDGSPGERGYTGLKGNTGPQG 798
            |..|:.|..|.||.          :|..|..|:||.||||   |..|..||.|..|.:|:.||.|
Human   967 GPPGKDGLPGHPGQ----------RGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPG 1021

  Fly   799 PPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGI-SRPGPMGPPGLN 862
            |||.:|..||.|..|.||:.|..|:||..|..||||.||..|.|||.|..|: ...||.||||..
Human  1022 PPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPA 1086

  Fly   863 GLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGV 927
            |..||:|..|..||||.||..|..|.||..|:.|.||..|..|..|..|..||:|..|.|||.|.
Human  1087 GSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGP 1151

  Fly   928 PGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPK---GFAGVTGAPGKRGPAGIPGVSG 989
            |      |.||.|||.|.||..|..|:||::|.||..||:   |..|.:||.|:.||.|..|:.|
Human  1152 P------GEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFG 1210

  Fly   990 AKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPP 1054
            .|||:|..|..|..||||.:|.||.||..|..||.|..|.||.      ||.:|..|.||.|||.
Human  1211 QKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGP------PGPRGPSGAPGADGPQ 1269

  Fly  1055 GLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRD 1119
            |.||..      |.||..|.:|:.|.||.||.|||.|.|        ||.||:|::|.||..|..
Human  1270 GPPGGI------GNPGAVGEKGEPGEAGEPGLPGEGGPP--------GPKGERGEKGESGPSGAA 1320

  Fly  1120 GINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGL 1184
            |..|.||..      |..|.|||.|..|.||.||..|.||.||..|.   |||:||.||||.:|.
Human  1321 GPPGPKGPP------GDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGP---PGDKGDDGEPGQTGS 1376

  Fly  1185 PGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLT 1249
            |      ||      ||.|||.|..|.||.|| ||                  |.:|.|||:
Human  1377 P------GP------TGEPGPSGPPGKRGPPG-PA------------------GPEGRQGEK--- 1407

  Fly  1250 GPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLI 1314
                  ||||:.||:||||.:         |.|||              :|.||:||.:|.:|:
Human  1408 ------GAKGEAGLEGPPGKT---------GPIGP--------------QGAPGKPGPDGLRGI- 1442

  Fly  1315 GAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGF 1379
              ||.:||:|||   |.||..|.|||:||                     ||.||||||:||
Human  1443 --PGPVGEQGLP---GSPGPDGPPGPMGP---------------------PGLPGLKGDSGP--- 1478

  Fly  1380 KGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDG 1444
                        ||:||..||.|..|.||..|:|||.|.||..|:.||   .||:|.|||.|..|
Human  1479 ------------KGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGP---KGEQGITGPSGPIG 1528

  Fly  1445 RDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGET 1509
            ..|.|||||         ||||||..|..|..|||||.|.||.
Human  1529 PPGPPGLPG---------PPGPKGAKGSSGPTGPKGEAGHPGP---------------------- 1562

  Fly  1510 GNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGH 1574
                 |||.|..||..:                  |.|                      ..|..
Human  1563 -----PGPPGPPGEVIQ------------------PLP----------------------IQASR 1582

  Fly  1575 TELWTGYSLLYVDGN-----DYAHNQD--LGSPGSCVPRFSTL---------PVLSCGQNNVCNY 1623
            |......|.|..|||     |||...:  .||..|.......:         |..:|....:| :
Human  1583 TRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLC-H 1646

  Fly  1624 ASRNDKTFWLTTN 1636
            ....|..:|:..|
Human  1647 PDFPDGEYWVDPN 1659

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	23/66 (35%)
		Collagen	322..380	CDD:189968	28/57 (49%)
		Collagen	413..465	CDD:189968	27/51 (53%)
		Collagen	499..561	CDD:189968	21/61 (34%)
		Collagen	574..632	CDD:189968	27/57 (47%)
		Collagen	657..714	CDD:189968	25/56 (45%)
		Collagen	765..824	CDD:189968	30/61 (49%)
		Collagen	854..911	CDD:189968	28/56 (50%)
		Collagen	884..943	CDD:189968	26/58 (45%)
		Collagen	923..982	CDD:189968	28/61 (46%)
		Collagen	1028..1085	CDD:189968	23/56 (41%)
		Collagen	1229..1287	CDD:189968	19/57 (33%)
		Collagen	1318..1376	CDD:189968	23/57 (40%)
		Collagen	1399..1458	CDD:189968	30/58 (52%)
		Collagen	1477..1534	CDD:189968	14/56 (25%)
		C4	1555..1662	CDD:128421	19/98 (19%)
		C4	1663..1777	CDD:128421
COL5A1	NP_000084.3	TSPN	39..230	CDD:214560
		Nonhelical region	231..443		23/124 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	242..269
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	281..457		31/138 (22%)
		Interrupted collagenous region	444..558		46/160 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	470..520		27/85 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	526..545		5/18 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	559..1574		540/1287 (42%)
		Triple-helical region	559..1570		538/1283 (42%)
		Collagen	726..>767	CDD:189968	22/40 (55%)
		Collagen	871..930	CDD:189968	28/70 (40%)
		Collagen	1474..1519	CDD:189968	26/62 (42%)
		Nonhelical region	1571..1605		11/73 (15%)
		COLFI	1610..1836	CDD:279718	9/51 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810