DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and COL4A4

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_000083.3	Gene:	COL4A4 / 1286	HGNCID:	2206	Length:	1690	Species:	Homo sapiens

Alignment Length:	1809	Identity:	788/1809 - (43%)
Similarity:	950/1809 - (52%)	Gaps:	269/1809 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 KNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGE 135
            ::|:      |..|:.|||:||.|||.||.|..|.:|.||..|.||:||.:||.||.|..||||:
Human    50 RDCS------VCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGD 108

  Fly   136 RGS---PGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKG 197
            :|.   ||..|..|:||..||.|..|.||::|.:|..|..|.||..|..|..||.|:.|:   ||
Human   109 KGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGE---KG 170

  Fly   198 EKGEPAKENGDY--AKGEKGEPG-------WRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEH 253
            |||......|..  .:|::|:||       | |..|.|||.|:|||      .|..|..|..|..
Human   171 EKGNSVFILGAVKGIQGDRGDPGLPGLPGSW-GAGGPAGPTGYPGE------PGLVGPPGQPGRP 228

  Fly   254 GLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPE 318
            ||||..|....|.|  |.||..|::|.|..:..|:|....: .:|:.|.||.||:||..|.||.:
Human   229 GLKGNPGVGVKGQM--GDPGEVGQQGSPGPTLLVEPPDFCL-YKGEKGIKGIPGMVGLPGPPGRK 290

  Fly   319 GDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGK 383
            |::|: |.|||||:||.||.||..|::|.||..|.||:.|..|..||.|..|.||:||..|..|.
Human   291 GESGI-GAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGP 354

  Fly   384 PGLL--------GPPGPPG-----GGRGTPGPPGPKG----P----RGYVGAPGPQGLNGVDGLP 427
            ||:|        ||||.||     |..|..|||||.|    |    .|.:|.|||||..|:.|||
Human   355 PGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLP 419

  Fly   428 GPQGYNGQKGGA-GLPGRPGNEGPPGKKGEKGTAGLN---------GPKGSIGPIGHPGPPGPEG 482
            |..|..|:...| |.||:||:.|.||..|.:|..|.:         ||:|..|.:|.||..||:|
Human   420 GEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKG 484

  Fly   483 QKGDAGL----PGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPG 543
            :||:.||    |     |..|..|.||.||.:||||:.|..|..|..||.  |.||..|..|.||
Human   485 EKGNEGLCACEP-----GPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDP--GPPGAEGPPGLPG 542

  Fly   544 QKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGER 608
            :.|.:|.||..|.||||.:         |...|.||::|..|.||.||:.|:.|..|..|..|:.
Human   543 KHGASGPPGNKGAKGDMVV---------SRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDP 598

  Fly   609 GHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGA 673
            |..| :.:...||.||..|..|.||..|..|.|.|             |:||.||.      :|.
Human   599 GPPG-DHEDATPGGKGFPGPLGPPGKAGPVGPPGL-------------GFPGPPGE------RGH 643

  Fly   674 EGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGS 738
            .|:||.||.:|..|.||:|| .....|...|||.|..|:.|.||..     ||.|..||.|..||
Human   644 PGVPGHPGVRGPDGLKGQKG-DTISCNVTYPGRHGPPGFDGPPGPK-----GFPGPQGAPGLSGS 702

  Fly   739 FGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVE 803
               .|.||.||:                ||...:|||||..|..|:.|:.|.||:: |.||||..
Human   703 ---DGHKGRPGT----------------PGTAEIPGPPGFRGDMGDPGFGGEKGSS-PVGPPGPP 747

  Fly   804 GPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEK 868
            |..|:||.:|..|:. |.|..|.|||.||.|:||..   ||||:||.  ||..||.|:.|..|.|
Human   748 GSPGVNGQKGIPGDP-AFGHLGPPGKRGLSGVPGIK---GPRGDPGC--PGAEGPAGIPGFLGLK 806

  Fly   869 GDRGPTGPIGFPGADGSVGY------PGDRGDAGLPGVSGRPGIVGEK---GDVGPIGPAGVAGP 924
            |.:|..|..||||..|..|:      ||..|..||||..|.||..|.|   |||||.||||:.|.
Human   807 GPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGL 871

  Fly   925 PGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSG 989
            ||:|      ||.||.|.||.||:   ||..||.|.||..|||            ||.|:||..|
Human   872 PGLP------GRPGAHGPPGLPGI---PGPFGDDGLPGPPGPK------------GPRGLPGFPG 915

  Fly   990 AKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPP 1054
            ..|:               ||.|||.|..|.||:      ||::|:.|:||::|.:|..|..|||
Human   916 FPGE---------------RGKPGAEGCPGAKGE------PGEKGMSGLPGDRGLRGAKGAIGPP 959

  Fly  1055 GLPGD---ASEKGQKGEPGP---SGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPG-EKGDQGR 1112
            |..|:   .|:||..|||||   .|..|:.|..||||..|.:|.|     ||.|||| .:|:.|.
Human   960 GDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEP-----GRYGPPGFHRGEPGE 1019

  Fly  1113 SGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYP----GDR 1173
            .|..|..|..|..|..||:|..|.||..|..|.||.||.||..|:.||.|..|..|.|    |..
Human  1020 KGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPP 1084

  Fly  1174 GDKGEPGLSGLP---GLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERG 1235
            |.|||||..|.|   |..||.|..|:||..|:||..|..|..|.||.|       ||.|..|.||
Human  1085 GPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCP-------GDHGMPGLRG 1142

  Fly  1236 YTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKG--------DIGPRGEIGYPG 1292
            ..||.|:.|.|||.|..|:.|..|.:|..|.||.:||:|:.|.||        |:||.|.:|.||
Human  1143 QPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPG 1207

  Fly  1293 VTIKGEKGLPGRPGRN--GRQGLIGAPGLIGERGLPGLA-------------GEPGLVGLPGPIG 1342
              :|||:|.||.||.:  |.:|..|.||..|..|.||.|             |.||..|.|||.|
Human  1208 --LKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDG 1270

  Fly  1343 PAGSKGERGLAGS----PGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGP 1403
            |.|:.|..||.||    .|:||..|.||.||..|..||.|:||..|.:|.:||||..|..|.|||
Human  1271 PRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGP 1335

  Fly  1404 SGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKG 1468
            .|.||..|:|   |.||..|..||.|.||.||..||..  ..|..|.:||..|.|||..|.|..|
Human  1336 HGFPGPPGEK---GLPGPPGRKGPTGLPGPRGEPGPPA--DVDDCPRIPGLPGAPGMRGPEGAMG 1395

  Fly  1469 EPGQPGRNGP--KGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEG 1531
            .||..|.:||  |||||..|.||:.|:.|..|..|.:|..||.|..|.|||.|..|:||.:|:
Human  1396 LPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGF-- 1458

  Fly  1532 AIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQD 1596
                     .||        ||.|.|:..|||::..|.|..|...|||||||||::|.:.|||||
Human  1459 ---------GPG--------YLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQD 1506

  Fly  1597 LGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEA 1661
            ||..|||:|.|||||...|..:.||:||.|||:::||.:.|.:||||:....||.|:|||.||||
Human  1507 LGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEA 1571

  Fly  1662 PANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNG 1726
            ||..:|||||...:|.||..|..|||||||||||..|:.||||||.|||||||||||.||:||.|
Human  1572 PAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQG 1636

  Fly  1727 AKGTCHFYETMTSFWMYNLESSQPF-ERPQQQTIKAGERQ-SHVSRCQVCMKNS 1778
            .:|||||:....|||:..:::...| ..|...|:|..:.| ..:||||||:|.|
Human  1637 RQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS 1690

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	29/59 (49%)
		Collagen	322..380	CDD:189968	30/57 (53%)
		Collagen	413..465	CDD:189968	25/61 (41%)
		Collagen	499..561	CDD:189968	30/61 (49%)
		Collagen	574..632	CDD:189968	22/57 (39%)
		Collagen	657..714	CDD:189968	23/56 (41%)
		Collagen	765..824	CDD:189968	26/58 (45%)
		Collagen	854..911	CDD:189968	28/65 (43%)
		Collagen	884..943	CDD:189968	32/67 (48%)
		Collagen	923..982	CDD:189968	24/58 (41%)
		Collagen	1028..1085	CDD:189968	27/62 (44%)
		Collagen	1229..1287	CDD:189968	28/65 (43%)
		Collagen	1318..1376	CDD:189968	30/74 (41%)
		Collagen	1399..1458	CDD:189968	27/58 (47%)
		Collagen	1477..1534	CDD:189968	27/58 (47%)
		C4	1555..1662	CDD:128421	58/106 (55%)
		C4	1663..1777	CDD:128421	63/115 (55%)
COL4A4	NP_000083.3	7S domain	39..64		6/19 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	61..173		56/114 (49%)
		Triple-helical region	65..1459		652/1547 (42%)
		Cell attachment site. /evidence=ECO:0000255	94..96		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	145..147		0/1 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	187..258		31/79 (39%)
		Cell attachment site. /evidence=ECO:0000255	189..191		0/1 (0%)
		Collagen	296..355	CDD:189968	31/58 (53%)
		Cell attachment site. /evidence=ECO:0000255	310..312		1/1 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	369..390		10/20 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	405..451		23/45 (51%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	469..1457		472/1116 (42%)
		Cell attachment site. /evidence=ECO:0000255	724..726		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	785..787		1/1 (100%)
		Collagen	829..880	CDD:189968	27/56 (48%)
		Collagen	914..964	CDD:189968	24/70 (34%)
		Cell attachment site. /evidence=ECO:0000255	989..991		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1212..1214		0/1 (0%)
		C4	1466..1570	CDD:279721	55/103 (53%)
		C4	1574..1686	CDD:279721	60/111 (54%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	164	1.000	Domainoid score	I3938
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	7	6.820