DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and COL4A3

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_000082.2	Gene:	COL4A3 / 1285	HGNCID:	2204	Length:	1670	Species:	Homo sapiens

Alignment Length:	1801	Identity:	788/1801 - (43%)
Similarity:	934/1801 - (51%)	Gaps:	258/1801 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    75 AGYAGCVPK----CI-----AEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQR 130
            |...|||.|    |.     .|||.:|.|||.|..|.||..|..|:.||.|.||..|.||..|.:
Human    26 AASKGCVCKDKGQCFCDGAKGEKGEKGFPGPPGSPGQKGFTGPEGLPGPQGPKGFPGLPGLTGSK 90

  Fly   131 GDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGS 195
            |.:|..|.||..|..|:||..|..|..|..|:.|..|..|:.|.|||.|..|.||..|.|   |.
Human    91 GVRGISGLPGFSGSPGLPGTPGNTGPYGLVGVPGCSGSKGEQGFPGLPGTLGYPGIPGAA---GL 152

  Fly   196 KGEKGEPAKENG---DYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPY---GAKGPRGEHG 254
            ||:||.||||..   | |||:.|.||..|..||.||.||||..|..|..|.:   ||.||||..|
Human   153 KGQKGAPAKEEDIELD-AKGDPGLPGAPGPQGLPGPPGFPGPVGPPGPPGFFGFPGAMGPRGPKG 216

  Fly   255 LKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVM----GPRGDMGQKGEPGLVGRKGEP 315
            ..||:           ..|.|||:|....:.|..|..||:    ||......|||.|..|..|||
Human   217 HMGER-----------VIGHKGERGVKGLTGPPGPPGTVIVTLTGPDNRTDLKGEKGDKGAMGEP 270

  Fly   316 GPEGDTGLDGQK--GEKGLPGGPGDRGRQGNFGP---PGSTGQKGDRGEPGLNGLPGNPGQKGEP 375
            ||.|.:||.|:.  .|||.||.||.:|:.|..|.   |||.|.||:||.|||.|..|..||||:
Human   271 GPPGPSGLPGESYGSEKGAPGDPGLQGKPGKDGVPGFPGSEGVKGNRGFPGLMGEDGIKGQKGD- 334

  Fly   376 GRAGATGKPGLLGPPGPPG-----------GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGP 429
                       :||||..|           |..||||||||:|.||..|..||.|:.|..|...|
Human   335 -----------IGPPGFRGPTEYYDTYQEKGDEGTPGPPGPRGARGPQGPSGPPGVPGSPGSSRP 388

  Fly   430 QGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGP-------EGQKGDA 487
             |..|..|..||.|..|..|.|||.. .||.|..|..||.|..|.||||||       :|..||.
Human   389 -GLRGAPGWPGLKGSKGERGRPGKDA-MGTPGSPGCAGSPGLPGSPGPPGPPGDIVFRKGPPGDH 451

  Fly   488 GLPGY-------GIQGSKGD--------------AGIPGYPGLKGSK------GERGFKGNAGAP 525
            |||||       |:.|.||:              .|:||.|||.|.|      |..|.||:.|:|
Human   452 GLPGYLGSPGIPGVDGPKGEPGLLCTQCPYIPGPPGLPGLPGLHGVKGIPGRQGAAGLKGSPGSP 516

  Fly   526 GDSKL-GRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGI 589
            |::.| |.||.|||.|.||.||:.|....|  :|.:|:.||.|.:      |..|.||..|:||.
Human   517 GNTGLPGFPGFPGAQGDPGLKGEKGETLQP--EGQVGVPGDPGLR------GQPGRKGLDGIPGT 573

  Fly   590 PGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLP--GATGEPGKPALCDLSLIEPL 652
            ||..|..||.||....||:|..|..|..|.||..|..|..|.|  |..||||            |
Human   574 PGVKGLPGPKGELALSGEKGDQGPPGDPGSPGSPGPAGPAGPPGYGPQGEPG------------L 626

  Fly   653 KGDKGYPGAPGAKGVQGFKG-------AEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRD 710
            :|.:|.|||||..|..|.:|       ..|.||.|||.|..|.:|..|:.|:.|..|.||..|.|
Human   627 QGTQGVPGAPGPPGEAGPRGELSVSTPVPGPPGPPGPPGHPGPQGPPGIPGSLGKCGDPGLPGPD 691

  Fly   711 GYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAK----GNKGEPGQTG 771
            |.|||||....|.||..|..|..|.|||.|..|:.||||        :|.|    |.||||. ..
Human   692 GEPGIPGIGFPGPPGPKGDQGFPGTKGSLGCPGKMGEPG--------LPGKPGLPGAKGEPA-VA 747

  Fly   772 MPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIP 836
            |||.||..|.|||||.:|..|..|..|.||:.                  |.|||.|.||.||.|
Human   748 MPGGPGTPGFPGERGNSGEHGEIGLPGLPGLP------------------GTPGNEGLDGPRGDP 794

  Fly   837 GRNGQPGPRGEPGISRPGPMGP---PGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLP 898
            |:.|.||.:|.||....||.|.   ||||||:|::|.||.|||.|.||..|.     ||  :|.|
Human   795 GQPGPPGEQGPPGRCIEGPRGAQGLPGLNGLKGQQGRRGKTGPKGDPGIPGL-----DR--SGFP 852

  Fly   899 GVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGN 963
            |.:|.|||.|.:|::||:|..|.   ||.|||.|..|.||..|..|.||.:|.|      |.|||
Human   853 GETGSPGIPGHQGEMGPLGQRGY---PGNPGILGPPGEDGVIGMMGFPGAIGPP------GPPGN 908

  Fly   964 DGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAG 1028
                     .|.||:||..|||||.|.:|..||.|..|:      :|.||...:..:.||:   |
Human   909 ---------PGTPGQRGSPGIPGVKGQRGTPGAKGEQGD------KGNPGPSEISHVIGDK---G 955

  Fly  1029 APGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLP 1093
            .||.:|..|.||||||:|.||:.|..||      ||..|..||.|.|||.|..|.||.||.:|:|
Human   956 EPGLKGFAGNPGEKGNRGVPGMPGLKGL------KGLPGPAGPPGPRGDLGSTGNPGEPGLRGIP 1014

  Fly  1094 GLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPG-----EKGSVGAPGIPGAPG 1153
            |..  |..|.||.||.:|..|..||.|..|..|..||||..|:||     ..|..|..|.||.||
Human  1015 GSM--GNMGMPGSKGKRGTLGFPGRAGRPGLPGIHGLQGDKGEPGYSEGTRPGPPGPTGDPGLPG 1077

  Fly  1154 MDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLP 1218
            ..|..|..|.||..|:.|..|.:|.||..|.|||.|:.||.|..||.|..|..|..||||.||||
Human  1078 DMGKKGEMGQPGPPGHLGPAGPEGAPGSPGSPGLPGKPGPHGDLGFKGIKGLLGPPGIRGPPGLP 1142

  Fly  1219 ATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRG-LQGPPGASGLNGIPGAKGDI 1282
                      |..|..|..|.:|:||..|:.||   ||.||:.| |:.|||..|..|..|||||
Human  1143 ----------GFPGSPGPMGIRGDQGRDGIPGP---AGEKGETGLLRAPPGPRGNPGAQGAKGD- 1193

  Fly  1283 GPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPI--GPAG 1345
                         :|..|.||.|||.      ||.|..|.||..|:.|.||..||||.|  |..|
Human  1194 -------------RGAPGFPGLPGRK------GAMGDAGPRGPTGIEGFPGPPGLPGAIIPGQTG 1239

  Fly  1346 SKGERGLAGSPGQPGQDGFPGAP--GLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPG 1408
            ::|..|..||||.||..|.||:.  |:|||.|..|..|.:|.   .|..||.|..|..|..|.||
Human  1240 NRGPPGSRGSPGAPGPPGPPGSHVIGIKGDKGSMGHPGPKGP---PGTAGDMGPPGRLGAPGTPG 1301

  Fly  1409 LVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQP 1473
            |.|.:||.|:.|..|..|..|.||..|..||.|..|..|.||:.|..|...::..||..|.||.|
Human  1302 LPGPRGDPGFQGFPGVKGEKGNPGFLGSIGPPGPIGPKGPPGVRGDPGTLKIISLPGSPGPPGTP 1366

  Fly  1474 GRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQ 1538
            |..|.:||||.||..|.:|..|.||:.|:.|..|..|..|..|.||.:||||..|.:|..||.|:
Human  1367 GEPGMQGEPGPPGPPGNLGPCGPRGKPGKDGKPGTPGPAGEKGNKGSKGEPGPAGSDGLPGLKGK 1431

  Fly  1539 KGEPGAPAPAALDYLT-GILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGS 1602
            :|:.|:||    .:.| |.:.|||||:..:|:|..|...|::|:|.|:|.||..||.||||:.||
Human  1432 RGDSGSPA----TWTTRGFVFTRHSQTTAIPSCPEGTVPLYSGFSFLFVQGNQRAHGQDLGTLGS 1492

  Fly  1603 CVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIP--MMPVENIEIRQYISRCVVCEAPANV 1665
            |:.||:|:|.|.|..|:|||:|||||.::||:|.|.:|  |.|:....:..|||||.|||.||..
Human  1493 CLQRFTTMPFLFCNVNDVCNFASRNDYSYWLSTPALMPMNMAPITGRALEPYISRCTVCEGPAIA 1557

  Fly  1666 IAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGT 1730
            |||||||.::|.||:||..||.|:||:|.|:.|:.|.||||.|||||||:|||:||:||:| :||
Human  1558 IAVHSQTTDIPPCPHGWISLWKGFSFIMFTSAGSEGTGQALASPGSCLEEFRASPFLECHG-RGT 1621

  Fly  1731 CHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMK 1776
            |::|....|||:.:|...:.|.:|...|:||||.:..:||||||||
Human  1622 CNYYSNSYSFWLASLNPERMFRKPIPSTVKAGELEKIISRCQVCMK 1667

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	24/56 (43%)
		Collagen	322..380	CDD:189968	30/62 (48%)
		Collagen	413..465	CDD:189968	21/51 (41%)
		Collagen	499..561	CDD:189968	31/82 (38%)
		Collagen	574..632	CDD:189968	25/57 (44%)
		Collagen	657..714	CDD:189968	27/63 (43%)
		Collagen	765..824	CDD:189968	21/58 (36%)
		Collagen	854..911	CDD:189968	30/59 (51%)
		Collagen	884..943	CDD:189968	26/58 (45%)
		Collagen	923..982	CDD:189968	24/58 (41%)
		Collagen	1028..1085	CDD:189968	28/56 (50%)
		Collagen	1229..1287	CDD:189968	26/58 (45%)
		Collagen	1318..1376	CDD:189968	30/61 (49%)
		Collagen	1399..1458	CDD:189968	26/58 (45%)
		Collagen	1477..1534	CDD:189968	26/56 (46%)
		C4	1555..1662	CDD:128421	55/108 (51%)
		C4	1663..1777	CDD:128421	64/114 (56%)
COL4A3	NP_000082.2	7S domain	29..42		5/12 (42%)
		Triple-helical region	43..1438		659/1548 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	49..78		14/28 (50%)
		Collagen	67..124	CDD:189968	24/56 (43%)
		Collagen	97..155	CDD:189968	26/60 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	167..469		139/327 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	502..1442		455/1068 (43%)
		Cell attachment site. /evidence=ECO:0000255	791..793		1/1 (100%)
		Collagen	830..885	CDD:189968	31/64 (48%)
		Cell attachment site. /evidence=ECO:0000255	996..998		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	1154..1156		0/1 (0%)
		Collagen	1164..1219	CDD:189968	31/77 (40%)
		Cell attachment site. /evidence=ECO:0000255	1306..1308		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1345..1347		0/1 (0%)
		Collagen	1390..1438	CDD:189968	21/47 (45%)
		Epitope recognized by Goodpasture antibodies	1427..1444		7/20 (35%)
		Cell attachment site. /evidence=ECO:0000255	1432..1434		0/1 (0%)
		C4	1446..1552	CDD:279721	52/105 (50%)
		Required for the anti-angiogenic activity of tumstatin	1479..1557		42/77 (55%)
		C4	1556..1666	CDD:279721	60/110 (55%)
		Required for the anti-tumor cell activity of tumstatin	1610..1628		10/18 (56%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1354	1.000	Inparanoid score	I139
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1239
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	9	8.930