DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col1a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_031768.2	Gene:	Col1a1 / 12842	MGIID:	88467	Length:	1453	Species:	Mus musculus

Alignment Length:	1395	Identity:	563/1395 - (40%)
Similarity:	649/1395 - (46%)	Gaps:	305/1395 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    73 CTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERG 137
            |...|.....:.:..:|.:|.|||.||   :|.:|.||.:|..|..|..|.|||      .|..|
Mouse    86 CPEEYVSPNSEDVGVEGPKGDPGPQGP---RGPVGPPGRDGIPGQPGLPGPPGP------PGPPG 141

  Fly   138 SPGLHG-------------QAG--VPGVQGPAGN---PGAPGINGKDGCDGQDGIPGLEGLSGMP 184
            .|||.|             .||  |||..||:|.   ||.||..|..|..|..|.||..|.||..
Mouse   142 PPGLGGNFASQMSYGYDEKSAGVSVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGGSGPM 206

  Fly   185 GPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAK-- 247
            ||||..|..|..|:.||..|..   ..||:|.||.:|..||.|..|.||.||.||.||..|||
Mouse   207 GPRGPPGPPGKNGDDGEAGKPG---RPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGD 268

  Fly   248 ----GPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGL 308
                ||:||.|..||.||    |.:.|..|:.||:|.|             ||.|..|.:|..|.
Mouse   269 AGPAGPKGEPGSPGENGA----PGQMGPRGLPGERGRP-------------GPPGTAGARGNDGA 316

  Fly   309 VGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKG 373
            ||..|.|||.|.||..|..|..|..|..|.:|.:|:.||.|..|:.|..|..|..|..||||..|
Mouse   317 VGAAGPPGPTGPTGPPGFPGAVGAKGEAGPQGARGSEGPQGVRGEPGPPGPAGAAGPAGNPGADG 381

  Fly   374 EPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGG 438
            :||..||.|.||:.|.||.| |.||..||.||.||                  |||      ||.
Mouse   382 QPGAKGANGAPGIAGAPGFP-GARGPSGPQGPSGP------------------PGP------KGN 421

  Fly   439 AGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGI 503
            :|.||.|||:|..|.|||.|..|:.||         |||.|.||::|..|.|        |.:|:
Mouse   422 SGEPGAPGNKGDTGAKGEPGATGVQGP---------PGPAGEEGKRGARGEP--------GPSGL 469

  Fly   504 PGYPGLKGSKGERGFKGN---AGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGD 565
            ||.||.:|..|.|||.|.   ||..|.|  |..|.||.||..|..|:|||||
Mouse   470 PGPPGERGGPGSRGFPGADGVAGPKGPS--GERGAPGPAGPKGSPGEAGRPG------------- 519

  Fly   566 VGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTG 630
                    .||..|.||.:|.||.||.||..||      ||..|.||..|..||||.:|:.|..|
Mouse   520 --------EAGLPGAKGLTGSPGSPGPDGKTGP------PGPAGQDGRPGPAGPPGARGQAGVMG 570

  Fly   631 LP---GATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEK 692
            .|   |..|||||            .|::|.||.|||.|..|..|..|..|.|||.|..|.:||:
Mouse   571 FPGPKGTAGEPGK------------AGERGLPGPPGAVGPAGKDGEAGAQGAPGPAGPAGERGEQ 623

  Fly   693 GLSGAPGNDGTPGRAGRDGYPGIPG-QSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEI 756
            |.:|:||..|.||.||..|..|.|| |.:.|:.|..|..||:|::|..|..|.:|.||
Mouse   624 GPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPG------- 681

  Fly   757 KMPA--KGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQG 819
              ||  :||.|.||..|..|..|..|:||.:|..||      ||.||..|..||.||:|::|:.|
Mouse   682 --PAGPRGNNGAPGNDGAKGDTGAPGAPGSQGAPGL------QGMPGERGAAGLPGPKGDRGDAG 738

  Fly   820 AVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADG 884
            ..|..|:|||||.||:                 .||:||||..|..|:||:.||:||   ||..|
Mouse   739 PKGADGSPGKDGARGL-----------------TGPIGPPGPAGAPGDKGEAGPSGP---PGPTG 783

  Fly   885 SVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLV 949
            :.|.|||||:|                  ||.||||.|||||..      |:.|||||||..|:
Mouse   784 ARGAPGDRGEA------------------GPPGPAGFAGPPGAD------GQPGAKGEPGDTGV- 823

  Fly   950 GMPGNKGDRGAPGNDGPKGFAGV---TGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGP 1011
                 |||.|.||..||.|..|.   .||||.:||.|..|..||.|..||.|..|..||.|..||
Mouse   824 -----KGDAGPPGPAGPAGPPGPIGNVGAPGPKGPRGAAGPPGATGFPGAAGRVGPPGPSGNAGP 883

  Fly  1012 PGAPGLM------GIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPG 1070
            ||.||.:      |.:|:.|.||.||:.|..|.||..|.:|.||.|||.|.|         |.||
Mouse   884 PGPPGPVGKEGGKGPRGETGPAGRPGEVGPPGPPGPAGEKGSPGADGPAGSP---------GTPG 939

  Fly  1071 PSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWG 1135
            |.|:.|..|..|.||..||:|.|||     .||.||.|.||.||..|..|..|..|..||.   |
Mouse   940 PQGIAGQRGVVGLPGQRGERGFPGL-----PGPSGEPGKQGPSGSSGERGPPGPMGPPGLA---G 996

  Fly  1136 QPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFT 1200
            .|||.|..|:||..|:||.|      |||||.|..|:.|..|.||..|.||..|..||.|..|..
Mouse   997 PPGESGREGSPGAEGSPGRD------GAPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKNGDR 1055

  Fly  1201 GAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQG 1265
            |..||.|..|..|..|       .||..|.||.||..||.||||:|      |:.|.:|..||||
Mouse  1056 GETGPAGPAGPIGPAG-------ARGPAGPQGPRGDKGETGEQGDR------GIKGHRGFSGLQG 1107

  Fly  1266 PPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAG 1330
            |||:                                   ||..|.||..||.|..|.||.||.||
Mouse  1108 PPGS-----------------------------------PGSPGEQGPSGASGPAGPRGPPGSAG 1137

  Fly  1331 EP---GLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFK------GERGLN 1386
            .|   ||.||||||||.|.:|..|.:|..|.||..|.||.||  ..:|...|.      .|:..:
Mouse  1138 SPGKDGLNGLPGPIGPPGPRGRTGDSGPAGPPGPPGPPGPPG--PPSGGYDFSFLPQPPQEKSQD 1200

  Fly  1387 GFEGQKGDKG----DRGLQGPSGLPGLVGQ 1412
            |....:.|..    ||.|:..:.|..|..|
Mouse  1201 GGRYYRADDANVVRDRDLEVDTTLKSLSQQ 1230

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	27/74 (36%)
		Collagen	322..380	CDD:189968	23/57 (40%)
		Collagen	413..465	CDD:189968	18/51 (35%)
		Collagen	499..561	CDD:189968	29/64 (45%)
		Collagen	574..632	CDD:189968	27/57 (47%)
		Collagen	657..714	CDD:189968	28/56 (50%)
		Collagen	765..824	CDD:189968	25/58 (43%)
		Collagen	854..911	CDD:189968	24/56 (43%)
		Collagen	884..943	CDD:189968	24/58 (41%)
		Collagen	923..982	CDD:189968	28/61 (46%)
		Collagen	1028..1085	CDD:189968	24/56 (43%)
		Collagen	1229..1287	CDD:189968	22/57 (39%)
		Collagen	1318..1376	CDD:189968	31/60 (52%)
		Collagen	1399..1458	CDD:189968	4/14 (29%)
		Collagen	1477..1534	CDD:189968
		C4	1555..1662	CDD:128421
		C4	1663..1777	CDD:128421
Col1a1	NP_031768.2	VWC	31..86	CDD:214564	563/1395 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	94..1210		555/1365 (41%)
		Nonhelical region (N-terminal)	152..167		2/14 (14%)
		Triple-helical region	168..1181		522/1253 (42%)
		Collagen	225..284	CDD:189968	28/61 (46%)
		Collagen	258..317	CDD:189968	28/75 (37%)
		Collagen	486..545	CDD:189968	32/87 (37%)
		Collagen	525..584	CDD:189968	32/76 (42%)
		Collagen	657..714	CDD:189968	25/65 (38%)
		Collagen	705..755	CDD:189968	27/72 (38%)
		Cell attachment site. /evidence=ECO:0000255	734..736		0/1 (0%)
		Collagen	<784..822	CDD:189968	26/61 (43%)
		Collagen	1068..1123	CDD:189968	31/102 (30%)
		Cell attachment site. /evidence=ECO:0000255	1082..1084		1/1 (100%)
		Nonhelical region (C-terminal)	1182..1207		4/24 (17%)
		COLFI	1219..1452	CDD:279718	3/12 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.900