DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col5a2

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_031763.2	Gene:	Col5a2 / 12832	MGIID:	88458	Length:	1497	Species:	Mus musculus

Alignment Length:	1653	Identity:	597/1653 - (36%)
Similarity:	715/1653 - (43%)	Gaps:	447/1653 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    76 GYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPG 140
            |..|.||   ...|.||.|||.||         ||.:||.||:|.||.|||   ||.:|..|.||
Mouse   114 GEPGLVP---VVTGIRGRPGPAGP---------PGSQGPRGDRGPKGRPGP---RGPQGIDGEPG 163

  Fly   141 LHGQAGVPGVQGPAGNPGAPGING--------------KDGCDGQDGI-PGLEGLSGMPGPRGYA 190
            :.||   ||..||.|:|..||.:|              |.|...|.|: ||..|..|..||:|..
Mouse   164 VPGQ---PGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMPGSVGPVGPRGPQGLQ 225

  Fly   191 GQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGL 255
            ||.|..|..|.|         ||.||||..|..|..||:|.||:.||.|:.   |..|..||.|.
Mouse   226 GQQGGVGPAGPP---------GEPGEPGPMGPIGSRGPEGPPGKPGEDGEP---GRNGNTGEVGF 278

  Fly   256 KGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGD 320
            .|..||..: |..||.||:||.:|.                :|..|.|||.|..|.|||.||.|.
Mouse   279 SGSPGARGF-PGAPGLPGLKGHRGH----------------KGLEGPKGEIGAPGAKGEAGPTGP 326

  Fly   321 TGLDGQKGEKGLPG-----GP-GDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAG 379
            .|..|..|.:|:||     || |..|::|..|.||..|..|..|.||.:|.|||||.|||.|..|
Mouse   327 MGAMGPLGPRGMPGERGRLGPQGAPGKRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTG 391

  Fly   380 ATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGR 444
            |.                   ||.||:|.||..|.|||                  .|..||||.
Mouse   392 AR-------------------GPEGPQGQRGETGPPGP------------------AGSQGLPGA 419

  Fly   445 PGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGL 509
            .|.:|.||.||..|:||.:||.|..||   ||.|||:|..|.     .||:|..||.|:||:.|.
Mouse   420 VGTDGTPGAKGPTGSAGTSGPPGLAGP---PGSPGPQGSTGP-----QGIRGQSGDPGVPGFKGE 476

  Fly   510 KGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCR 574
            .|.|||                          ||..|..|..|.||::|               :
Mouse   477 AGPKGE--------------------------PGPHGIQGPIGPPGEEG---------------K 500

  Fly   575 AGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPG 639
            .||:||.||.|.||..|:   ||.||.||:||.   ||:.|..|..||:|..|.:|..|..|:||
Mouse   501 RGPRGDPGTVGPPGPMGE---RGAPGNRGFPGS---DGLPGPKGAQGERGPVGSSGPKGGQGDPG 559

  Fly   640 KPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTP 704
            :|               |.||.|||:|:      .|.||:.||:|:.      |..||||.||.|
Mouse   560 RP---------------GEPGLPGARGL------TGNPGVQGPEGKL------GPLGAPGEDGRP 597

  Fly   705 GRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQ 769
            |..|..|        |:|:||..|..|.||..|..|:.||                .||.|.|||
Mouse   598 GPPGSIG--------IRGQPGSMGLPGPKGSSGDLGKPGE----------------AGNAGVPGQ 638

  Fly   770 TGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGL---NGPRGEKGNQGAVGVPGNPGKDG 831
            .|.||..||.|..|..|..||.|..|.|||||..|.:||   .||.||.|..|..||||.||..|
Mouse   639 RGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKAGDQGVPGEPGAVG 703

  Fly   832 LRGIPGRNGQPGPRGEPGIS-RPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDA 895
            ..|..|..|.||.||||||: .||..|..|.:|..|.||:.||||.||..|..|..|.||:||.|
Mouse   704 PLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGNPGPTGTIGDTGPPGLQGMPGERGIA 768

  Fly   896 GLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGA 960
            |.||..|..|.:||||..|..|..|..|.||..|..|..|..|.|||||..||||.||::|:.|:
Mouse   769 GTPGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGS 833

  Fly   961 PGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQG 1025
            .|.:||   .|..|..|.:||.|.|||.|..|:.|..|..|:.||                  ||
Mouse   834 RGENGP---TGAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGP------------------QG 877

  Fly  1026 LAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEK 1090
            |||:||..|..|:||.||.:|..|..|..|.||.|   |:.|.|||:|..|..||||.|
Mouse   878 LAGSPGPHGPHGVPGLKGGRGTQGPPGATGFPGSA---GRVGPPGPAGAPGPAGPAGEP------ 933

  Fly  1091 GLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMD 1155
                    |:.||||.:||.|..                     |:.|::|..|.||.|
Mouse   934 --------GKEGPPGLRGDPGSH---------------------GRVGDRGPAGPPGSP------ 963

  Fly  1156 GLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPAT 1220
                              ||||:||..|.||..|..||.|..|..|..|..|:||.||.||||
Mouse   964 ------------------GDKGDPGEDGQPGPDGPPGPAGTTGQRGIVGMPGQRGERGMPGLP-- 1008

  Fly  1221 VPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPR 1285
                    |..|..|..|..|..|::|..||.|..|:.|..|..||.|.:|.:|.||..|.:|.|
Mouse  1009 --------GPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPAGNDGTPGRDGAVGER 1065

  Fly  1286 GEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGER 1350
            |:.|.|     |..||||..|..|..|.:||||..|:||.||..         ||:||.|..|:|
Mouse  1066 GDRGDP-----GPAGLPGSQGAPGTPGPVGAPGDAGQRGEPGSR---------GPVGPPGRAGKR 1116

  Fly  1351 GLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGD 1415
            ||                     .||||.:|::|.||..|.:|.||.||..|..||||..|..|:
Mouse  1117 GL---------------------PGPQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPNGE 1160

  Fly  1416 TGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKG 1480
            .|..|:.|..||.|.|      ||.|..|::|.||..|..|      |||.:|..|:.|..||.|
Mouse  1161 QGSAGIPGPFGPRGPP------GPVGPSGKEGNPGPLGPIG------PPGVRGSVGEAGPEGPPG 1213

  Fly  1481 EPGRPGERGLIG--------IQGERGEKGERGLIGETGNVGRPGPK--GDRGEPGERGYEGAIGL 1535
            |||.||..|..|        |.|...|           |:..|.|:  .|:..|.:.
Mouse  1214 EPGPPGPPGPPGHLTAALGDIMGHYDE-----------NMPDPLPEFTEDQAAPDDT-------- 1259

  Fly  1536 IGQKGEPGAPAPAALDYLTGILITRHSQSETV--PACSAGHTELWTGYSLLYVDGNDYAHNQDLG 1598
              .|.:||.       ::|  |.:..||.||:  |..|..|
Mouse  1260 --NKTDPGI-------HVT--LKSLSSQIETMRSPDGSKKH------------------------ 1289

  Fly  1599 SPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVE---NIEIRQYISRCVVCE 1660
                        |..:|....:| :.::....:|:..|.......::   |:|..:   .|:
Mouse  1290 ------------PARTCDDLKLC-HPTKQSGEYWIDPNQGSAEDAIKVYCNMETGE---TCI--- 1335

  Fly  1661 APANVIAVHSQT---IEVPDCPNGWEGL 1685
             .||..:|..:|   .:.||....|.||
Mouse  1336 -SANPASVPRKTWWASKSPDNKPVWYGL 1362

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	26/56 (46%)
		Collagen	322..380	CDD:189968	29/63 (46%)
		Collagen	413..465	CDD:189968	18/51 (35%)
		Collagen	499..561	CDD:189968	18/61 (30%)
		Collagen	574..632	CDD:189968	27/57 (47%)
		Collagen	657..714	CDD:189968	24/56 (43%)
		Collagen	765..824	CDD:189968	31/61 (51%)
		Collagen	854..911	CDD:189968	28/56 (50%)
		Collagen	884..943	CDD:189968	28/58 (48%)
		Collagen	923..982	CDD:189968	27/58 (47%)
		Collagen	1028..1085	CDD:189968	27/56 (48%)
		Collagen	1229..1287	CDD:189968	23/57 (40%)
		Collagen	1318..1376	CDD:189968	15/57 (26%)
		Collagen	1399..1458	CDD:189968	24/58 (41%)
		Collagen	1477..1534	CDD:189968	18/66 (27%)
		C4	1555..1662	CDD:128421	16/111 (14%)
		C4	1663..1777	CDD:128421	9/26 (35%)
Col5a2	NP_031763.2	VWC	40..95	CDD:214564
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	103..1265		569/1501 (38%)
		Cell attachment site. /evidence=ECO:0000255	141..143		0/1 (0%)
		Collagen	268..326	CDD:189968	28/74 (38%)
		Collagen	310..368	CDD:189968	25/57 (44%)
		Collagen	358..417	CDD:189968	33/95 (35%)
		Collagen	493..551	CDD:189968	30/78 (38%)
		Cell attachment site. /evidence=ECO:0000255	504..506		0/1 (0%)
		Collagen	523..581	CDD:189968	29/81 (36%)
		Collagen	589..647	CDD:189968	31/81 (38%)
		Collagen	700..757	CDD:189968	28/56 (50%)
		Collagen	730..787	CDD:189968	29/56 (52%)
		Collagen	817..875	CDD:189968	26/60 (43%)
		Cell attachment site. /evidence=ECO:0000255	942..944		0/1 (0%)
		Collagen	1042..1098	CDD:189968	27/60 (45%)
		Cell attachment site. /evidence=ECO:0000255	1065..1067		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	1068..1070		0/1 (0%)
		Collagen	1072..1131	CDD:189968	32/88 (36%)
		Cell attachment site. /evidence=ECO:0000255	1125..1127		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1134..1136		0/1 (0%)
		COLFI	1265..1496	CDD:279718	27/151 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810