DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col5a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_056549.2	Gene:	Col5a1 / 12831	MGIID:	88457	Length:	1838	Species:	Mus musculus

Alignment Length:	1435	Identity:	582/1435 - (40%)
Similarity:	675/1435 - (47%)	Gaps:	322/1435 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    94 PGPLGPTGLKGEMG--FPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGN 156
            |..:|| |:.....  |.|:.||.|:|||||:|...          .||:..: |.||.:||||.
Mouse   427 PSEIGP-GMPANQDTIFEGIGGPRGEKGQKGEPAII----------EPGMLIE-GPPGPEGPAGL 479

  Fly   157 PGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRG 221
            ||.||..|.                        .||:|..||:|.|            |.||..|
Mouse   480 PGPPGTTGP------------------------TGQMGDPGERGPP------------GRPGLPG 508

  Fly   222 TAGLAGPQG----FPGEKGERGDSGPYG--------------------AKGPRGEHGLKGEKGAS 262
            ..||.||.|    .|...|..||:|..|                    .:||.|..||.|..|.
Mouse   509 ADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGP- 572

  Fly   263 CYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQK 327
             .||  ||:.|:|||.|:             |||:|..|.:|.||..|:   ||..|..|.||.:
Mouse   573 -MGP--PGSGGLKGEPGD-------------MGPQGPRGVQGPPGPTGK---PGRRGRAGSDGAR 618

  Fly   328 GEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGP 392
            |..|..|..||||..|..|.|   |:||.||:||.:|.||.||..||.|..|..|..||.|.|||
Mouse   619 GMPGQTGPKGDRGFDGLAGLP---GEKGHRGDPGPSGPPGIPGDDGERGDDGEVGPRGLPGEPGP 680

  Fly   393 PG--GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKG 455
            .|  |.:|.||||||.|..|..|.|||:|..|..|.|||.|..|..|..||||..|..||||:||
Mouse   681 RGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKG 745

  Fly   456 EKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKG 520
            ..|..||.|..|:.||.||||..||.|:||..|.|     |.:|..|.||..|:||:.|.||.||
Mouse   746 PLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPP-----GPQGPIGYPGPRGVKGADGIRGLKG 805

  Fly   521 NAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSG 585
            ..|..|:.  |.||..|..|..|.:|:.|.||..|:.|..|.||..|........||.|:||..|
Mouse   806 TKGEKGED--GFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLG 868

  Fly   586 LPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIE 650
            :||:||..|.:||.|..|:||..|.:|..|..|.||:.|..|:.|..|..||
Mouse   869 VPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGE------------- 920

  Fly   651 PLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGI 715
              :|.:|..|.||.||..|..|..|.||..||      .|.:|.:|.||..|.||..|:||.||.
Mouse   921 --RGPRGITGKPGPKGNSGGDGPAGPPGERGP------NGPQGPTGFPGPKGPPGPPGKDGLPGH 977

  Fly   716 PGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDG 780
            |||  :||.||.|:.|..|..|..|..|..||.|.             .||.|..|.||||||.|
Mouse   978 PGQ--RGETGFQGKTGPPGPPGVVGPQGPTGETGP-------------MGERGHPGPPGPPGEQG 1027

  Fly   781 SPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPR 845
            .||..|..|.||:.||.|.||.:||                        .||||.||..|.|||.
Mouse  1028 LPGAAGKEGTKGDPGPAGLPGKDGP------------------------PGLRGFPGDRGLPGPV 1068

  Fly   846 GEPGI-SRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGE 909
            |..|: ...||.||||..|..||:|..|..||||.||..|..|.||..|:.||||..|..|..|.
Mouse  1069 GALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGLPGEKGPQGPAGR 1133

  Fly   910 KGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPK---GFAG 971
            .|..||:|..|.|||.|.|      |.||.|||.|.||..|..|:||::|.||..||:   |..|
Mouse  1134 DGLQGPVGLPGPAGPVGPP------GEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPG 1192

  Fly   972 VTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLD 1036
            .:||.|:.||.|..|:.|.|||:|:.|..|..||||.:|.||.||..|..||.|..|.||.
Mouse  1193 PSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGP---- 1253

  Fly  1037 GMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRA 1101
              ||.:|..|.||.|||.|.||..      |.||..|.:|:.|.||.||.|||        .|..
Mouse  1254 --PGPRGPSGAPGADGPQGPPGGI------GNPGAVGEKGEPGEAGDPGLPGE--------GGPL 1302

  Fly  1102 GPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGA 1166
            ||.||:|::|.:|..|..|..|.||..      |..|.|||.|..|.||.||..|.||.||..|.
Mouse  1303 GPKGERGEKGEAGPSGAAGPPGPKGPP------GDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGP 1361

  Fly  1167 VGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQ 1231
               |||:||.||||.:|.|      ||      ||.|||.|..|.||.|| ||
Mouse  1362 ---PGDKGDDGEPGQTGSP------GP------TGEPGPSGPPGKRGPPG-PA------------ 1398

  Fly  1232 GERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIK 1296
                  |.:|.|||:         ||||:.||:||||.:         |.|||
Mouse  1399 ------GPEGRQGEK---------GAKGEAGLEGPPGKT---------GPIGP------------ 1427

  Fly  1297 GEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQ 1361
              :|.||:||.:|.:|:   ||.:||:|||   |.||..|.|||:||
Mouse  1428 --QGAPGKPGPDGLRGI---PGPVGEQGLP---GSPGPDGPPGPMGP------------------ 1466

  Fly  1362 DGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDG 1426
               ||.||||||:||               ||:||..||.|..|.||..|:|||.|.||..|:.|
Mouse  1467 ---PGLPGLKGDSGP---------------KGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSG 1513

  Fly  1427 PVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGLI 1491
            |   .|::|.|||.|..|..|.|||||         ||||||..|..|..|||||.|.||..|..
Mouse  1514 P---KGDQGITGPSGPLGPPGPPGLPG---------PPGPKGAKGSSGPTGPKGEAGHPGLPGPP 1566

  Fly  1492 GIQGE 1496
            |..||
Mouse  1567 GPPGE 1571

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	22/58 (38%)
		Collagen	322..380	CDD:189968	28/57 (49%)
		Collagen	413..465	CDD:189968	27/51 (53%)
		Collagen	499..561	CDD:189968	26/61 (43%)
		Collagen	574..632	CDD:189968	26/57 (46%)
		Collagen	657..714	CDD:189968	24/56 (43%)
		Collagen	765..824	CDD:189968	24/58 (41%)
		Collagen	854..911	CDD:189968	29/56 (52%)
		Collagen	884..943	CDD:189968	27/58 (47%)
		Collagen	923..982	CDD:189968	28/61 (46%)
		Collagen	1028..1085	CDD:189968	23/56 (41%)
		Collagen	1229..1287	CDD:189968	19/57 (33%)
		Collagen	1318..1376	CDD:189968	23/57 (40%)
		Collagen	1399..1458	CDD:189968	29/58 (50%)
		Collagen	1477..1534	CDD:189968	12/20 (60%)
		C4	1555..1662	CDD:128421
		C4	1663..1777	CDD:128421
Col5a1	NP_056549.2	TSPN	39..230	CDD:214560
		Nonhelical region. /evidence=ECO:0000250	231..443		4/16 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	242..545		51/165 (31%)
		Interrupted collagenous region. /evidence=ECO:0000250	444..558		46/160 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	559..1574		531/1255 (42%)
		Triple-helical region. /evidence=ECO:0000250	559..1570		529/1252 (42%)
		Collagen	726..>767	CDD:189968	22/40 (55%)
		Collagen	796..855	CDD:189968	24/60 (40%)
		Collagen	829..886	CDD:189968	24/56 (43%)
		Collagen	871..930	CDD:189968	25/73 (34%)
		Nonhelical region. /evidence=ECO:0000250	1571..1605		1/1 (100%)
		COLFI	1610..1836	CDD:279718
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810