DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col4a4

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_031761.1	Gene:	Col4a4 / 12829	MGIID:	104687	Length:	1682	Species:	Mus musculus

Alignment Length:	1810	Identity:	803/1810 - (44%)
Similarity:	944/1810 - (52%)	Gaps:	271/1810 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 KNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGD---PGPYGQRGD 132
            :||:      |.:|..|||:||.||||||.|..|.:|..|..|..|:||::||   |||.|::||
Mouse    42 RNCS------VCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEKGD 100

  Fly   133 KGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKG 197
            ||..|.||..|..||||..||.|..|.||:   ||.:|..|.||..|..|.|||.|..||.|..|
Mouse   101 KGPTGVPGFPGVDGVPGHPGPPGPRGKPGV---DGYNGSRGDPGYPGERGAPGPGGPPGQPGENG 162

  Fly   198 EKGEPAKENGDY--AKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKG 260
            |||......|..  .:|::|:||..|..|..|.||.||..|..|..|..|..|..|..||||..
Mouse   163 EKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPGSPGLKGNP- 226

  Fly   261 ASCYGPMKPGAPGIKGEKGEP---ASSFPVKPTHTVMGP-----RGDMGQKGEPGLVGRKGEPGP 317
                      |.|:||::|||   ....|..||..|..|     :|:.|.||.||::|..|.||.
Mouse   227 ----------ATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPPGPPGR 281

  Fly   318 EGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATG 382
            :|..|: |.|||||:||.||.||..|:.||||..|.||.:|..|..||.|..|.||:.|..|..|
Mouse   282 KGAPGV-GIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGPKGDLGDRGYPG 345

  Fly   383 KPGLL-----------GPPGPPG-----GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQG 431
            .||:|           |.|||||     |..|.||||||.|..|.. .||..|..|..|:|||.|
Mouse   346 PPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGET-CPGMMGPPGPPGVPGPPG 409

  Fly   432 YNGQKGGAGLPGR----PGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPG- 491
            :.|:   ||:|||    ||..|.||..|..|..|..||.||......||.|||.|:||..|.||
Mouse   410 FPGE---AGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVIYCRPGCPGPMGEKGKVGPPGR 471

  Fly   492 YGIQGSKGDAGI-------------PGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPG 543
            .|.:|:||:.|:             ||.||.:||||:.|..|..|..||.  |:||..|..|.||
Mouse   472 RGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGDLGLPGWHGEKGDP--GQPGAEGPPGPPG 534

  Fly   544 QKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIP---GKDGARGPPGERGYP 605
            :.|..|.||..|:||||.|         |...|.||::|..|.||.|   |:||..|.|||||.|
Mouse   535 RPGAMGPPGHKGEKGDMVI---------SRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDP 590

  Fly   606 GERG--HDGINGQTGPPGEKGEDGRTGL--PGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKG 666
            |.||  .|...|:.|.||..|..||||.  |...|.||.|            |.:|.||.||..|
Mouse   591 GPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGLGFPGPP------------GQRGLPGEPGRPG 643

  Fly   667 VQGFKGAE--------------GLPGIPGPKGEFGFKGEKGLSGAPG---NDGTPGRAGRDGYPG 714
            .:||.|.:              |.||:||..|..|.||..|..||||   .||..|:.|:.|..|
Mouse   644 TRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGFPGPPGAPGMRCPDGQKGQRGKPGMSG 708

  Fly   715 IPGQSIKGEPGFHGRDGAKGDKGSFGRS--GEKGEPGSCALDEIKMPAK-GNKGEPGQT--GMPG 774
            ||     |.|||.|..|..|.||..|.|  |..|.|||        |.| |.||.||..  |.||
Mouse   709 IP-----GPPGFRGDMGDPGIKGEKGTSPIGPPGPPGS--------PGKDGQKGIPGDPAFGDPG 760

  Fly   775 PPGEDGSPGERGYTGLKGN---TGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIP 836
            ||||.|.||..|..|.||:   .|..||||:.|..||.||:|.:|::|..|:||:||....||.|
Mouse   761 PPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAP 825

  Fly   837 GRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVS 901
            |..||||        .||..|.||..|.:|:.||.||:||.|..|..|..|.||..|..|.||:
Mouse   826 GIPGQPG--------LPGTPGDPGAPGWKGQPGDMGPSGPAGMKGLPGLPGLPGADGLRGPPGI- 881

  Fly   902 GRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLV---GMPGNKGD---RGA 960
              ||..||.|..|..|..|:.|.||.||..|.||:.|..||||..|.|   |.||.|||   |||
Mouse   882 --PGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGKPGPDGEPGRKGEVGEKGWPGLKGDLGERGA 944

  Fly   961 PGNDGPKGFAG--VT---GAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGI 1020
            .|:.|..|.||  ||   |.||..||.|..|.||.:||||::|:.      ||||.||..||.|:
Mouse   945 KGDRGLPGDAGEAVTSRKGEPGDAGPPGDGGFSGERGDKGSSGMR------GGRGDPGRDGLPGL 1003

  Fly  1021 -KGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTP 1084
             :|..|:.|.||.   .|.||..|:.|..|:.|.||.|||   :|..|.|||.|..||.|..|..
Mouse  1004 HRGQPGIDGPPGP---PGPPGPPGSPGLRGVIGFPGFPGD---QGDPGSPGPPGFPGDDGARGPK 1062

  Fly  1085 GWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEK---GEQGLQGVWGQPGEKGSVGAP 1146
            |:.|:..       .:.||||.||:.|..|..||.|:.|||   |::|.:|..|:||:.||.|.|
Mouse  1063 GYKGDPA-------SQCGPPGPKGEPGSPGYQGRTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPP 1120

  Fly  1147 GIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGI 1211
            |.||.|||   ||..|.||.||.||.|||.|:   .|.||..|..||:|..|..|..|.|||:|.
Mouse  1121 GCPGDPGM---PGLKGHPGEVGDPGPRGDAGD---FGRPGPAGVKGPLGSPGLNGLHGLKGEKGT 1179

  Fly  1212 RGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIP 1276
            :|..||.                          |.|..||.|:.|.||::|..|.||
Mouse  1180 KGASGLL--------------------------EMGPPGPMGMPGQKGEKGDPGSPG-------- 1210

  Fly  1277 GAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPI 1341
                 |.|.|        :.||||.||.|||.|..|..||||...:..:|. .|.||..|.|||.
Mouse  1211 -----ISPPG--------LPGEKGFPGPPGRPGPPGPAGAPGRAAKGDIPD-PGPPGDRGPPGPD 1261

  Fly  1342 GPAGSKGERGLAGS----PGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQG 1402
            ||.|..|..|..|:    .|.||..|.||.||.:|..||.|.:|..|.:|.:||||..|..||.|
Mouse  1262 GPRGVPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLPGLPG 1326

  Fly  1403 PSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPK 1467
            |.||||..|:|   |.||..|..||||.||.||..||..  ..|..|.:||..|.||...|.|..
Mouse  1327 PPGLPGAPGEK---GLPGPPGRKGPVGPPGCRGEPGPPA--DVDSCPRIPGLPGVPGPRGPEGAM 1386

  Fly  1468 GEPGQPGRNGP--KGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYE 1530
            ||||:.|..||  |||||..|.||..||.|..|..|.:|..||.|..|.|||.|..|:||.:|:
Mouse  1387 GEPGRRGLPGPGCKGEPGPDGRRGQDGIPGSPGPPGRKGDTGEAGCPGAPGPPGPTGDPGPKGF- 1450

  Fly  1531 GAIGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQ 1595
                      .||:        |:|.|:..|||::..|||..|...|||||||||::|.:.||||
Mouse  1451 ----------GPGS--------LSGFLLVLHSQTDQEPACPVGMPRLWTGYSLLYMEGQEKAHNQ 1497

  Fly  1596 DLGSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCE 1660
            |||..|||:|.|||||...|..:.||:||.|||:::||::.|.:||||:...|||.|||||.|||
Mouse  1498 DLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLSSAAPLPMMPLSEEEIRSYISRCAVCE 1562

  Fly  1661 APANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECN 1725
            |||..:|||||...:|.||..|..|||||||||||..|:.||||||.|||||||||||.||:||.
Mouse  1563 APAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFVECQ 1627

  Fly  1726 GAKGTCHFYETMTSFWMYNLESSQPF-ERPQQQTIKAGERQSH-VSRCQVCMKNS 1778
            |.:|||||:....|||:..:.....| ..|...|:|..:.|.. :||||||||:|
Mouse  1628 GRQGTCHFFANEYSFWLTTVNPDLQFASGPSPDTLKEVQAQRRKISRCQVCMKHS 1682

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	29/59 (49%)
		Collagen	322..380	CDD:189968	30/57 (53%)
		Collagen	413..465	CDD:189968	23/55 (42%)
		Collagen	499..561	CDD:189968	30/74 (41%)
		Collagen	574..632	CDD:189968	31/64 (48%)
		Collagen	657..714	CDD:189968	28/73 (38%)
		Collagen	765..824	CDD:189968	31/63 (49%)
		Collagen	854..911	CDD:189968	26/56 (46%)
		Collagen	884..943	CDD:189968	26/58 (45%)
		Collagen	923..982	CDD:189968	35/69 (51%)
		Collagen	1028..1085	CDD:189968	25/56 (45%)
		Collagen	1229..1287	CDD:189968	14/57 (25%)
		Collagen	1318..1376	CDD:189968	24/61 (39%)
		Collagen	1399..1458	CDD:189968	29/58 (50%)
		Collagen	1477..1534	CDD:189968	28/58 (48%)
		C4	1555..1662	CDD:128421	61/106 (58%)
		C4	1663..1777	CDD:128421	64/115 (56%)
Col4a4	NP_031761.1	7S domain. /evidence=ECO:0000250\|UniProtKB:P53420	31..56		7/19 (37%)
		Collagen	54..111	CDD:189968	30/56 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	56..255		92/212 (43%)
		Triple-helical region. /evidence=ECO:0000250\|UniProtKB:P53420	57..1451		663/1548 (43%)
		Cell attachment site. /evidence=ECO:0000255	86..88		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	137..139		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	181..183		0/1 (0%)
		Collagen	291..348	CDD:189968	29/56 (52%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	379..1453		517/1213 (43%)
		Cell attachment site. /evidence=ECO:0000255	587..589		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	593..595		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	716..718		0/1 (0%)
		Collagen	805..858	CDD:189968	27/60 (45%)
		Collagen	889..948	CDD:189968	29/58 (50%)
		Cell attachment site. /evidence=ECO:0000255	980..982		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	992..994		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	1144..1146		1/1 (100%)
		Collagen	1338..1405	CDD:189968	34/68 (50%)
		C4	1458..1562	CDD:279721	58/103 (56%)
		C4	1566..1679	CDD:279721	61/112 (54%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	146	1.000	Domainoid score	I4520
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.810