DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col4a3

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_031760.2	Gene:	Col4a3 / 12828	MGIID:	104688	Length:	1669	Species:	Mus musculus

Alignment Length:	1796	Identity:	795/1796 - (44%)
Similarity:	937/1796 - (52%)	Gaps:	257/1796 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    79 GCVPK------CIAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERG 137
            |||.|      |...||.:|..|..|..|..|:.||||.||..|.:|.||.||..|..|.||.||
Mouse    30 GCVCKGKGQCLCAGTKGEKGEKGVPGSPGFPGQKGFPGPEGLPGPQGPKGSPGLPGLTGPKGIRG 94

  Fly   138 SPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEP 202
            ..||.|.||.||:.|..|:||..|:.|..||:|..|..|..|..|.||..|..|..|.||:||||
Mouse    95 ITGLPGFAGPPGLPGLPGHPGPRGLAGLPGCNGSKGEQGFPGFPGTPGYAGLPGPDGLKGQKGEP 159

  Fly   203 A--KENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYG 265
            |  ::.|...||:.|.||..|..|..|..||||..|..|..|.:|..|..|..|.||..|.|..|
Mouse   160 AQGEDRGFNGKGDPGPPGVPGFQGFPGLPGFPGPAGPPGPPGFFGLPGAMGPRGPKGHMGDSVIG 224

  Fly   266 PMKPGAPGIKGEKGEPASSFPVKPTHTVM-------------GPRGDMGQKGEPGLVGRKGEPGP 317
              :.|..|:||..|.|.      |..||:             |.:||.|::|||      |.|||
Mouse   225 --QKGERGMKGLTGPPG------PPGTVIFTLTQPYNKSDFKGEKGDEGERGEP------GPPGP 275

  Fly   318 EGDTGLDGQKGEKGLPGGPGDRGRQGNFGP---PGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAG 379
            .|..| |....|||.||.||.||:.|..|.   ||:.|.||:||.|||.|..|..|:||:
Mouse   276 SGPPG-DSYGSEKGAPGEPGPRGKPGKDGAPGFPGTEGAKGNRGFPGLRGEAGIKGRKGD----- 334

  Fly   380 ATGKPGLLGPPGPPG-----------GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYN 433
                   :||||.||           |.||.||.|||||.||..|..||.|:.|..||..| |..
Mouse   335 -------IGPPGFPGPTEYYDAYLEKGERGMPGLPGPKGARGPQGPSGPPGVPGSPGLSRP-GLR 391

  Fly   434 GQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGL---PGYGIQ 495
            |..|..||.|..|..||||    |.|.|..||.|..|..|.||||||.|..||...   ||.  .
Mouse   392 GPIGWPGLKGSKGERGPPG----KDTVGPPGPLGCPGSPGPPGPPGPPGCPGDIVFKCSPGE--H 450

  Fly   496 GSKGDAGIPGYPGLKGSKGERG--------FKGNAGAPGDSKL-GRPGTPGAAGAPGQKGDAGRP 551
            |..||.|.||.|||.|.|||.|        |.|..|.||...| |..|.||..|.||.||:.|.|
Mouse   451 GMPGDTGPPGVPGLDGPKGEPGSPCTECHCFPGPPGVPGFPGLDGIKGIPGGRGVPGLKGNPGSP 515

  Fly   552 GTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLP----GIPGKDGARGPPGERGYPGERGHDG 612
            |:.|..|..|..||.|      ..|.|||||.:.||    |.||..|.||.||.:|:.|..|..|
Mouse   516 GSAGLPGFAGFPGDQG------HPGLKGDKGDTPLPWGQVGNPGDPGLRGLPGRKGFDGTPGGPG 574

  Fly   613 INGQTGPPGE------KGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFK 671
            ..|..||.||      ||:.|..|.||..|.|| ||           |..|.||. |.:|..|.|
Mouse   575 AKGPPGPQGEPALSGRKGDQGPPGPPGFPGPPG-PA-----------GPAGPPGY-GPQGEPGPK 626

  Fly   672 GAEGLPGIPGPKGEFGFKGEKGLS----GAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGA 732
            ||:|:||:.||.||.|.|||...|    |.||..|.||:||..|.||:||...|.:||..|.||.
Mouse   627 GAQGVPGVLGPPGEAGLKGEPSTSTPDLGPPGPPGPPGQAGPRGLPGLPGPVGKCDPGLPGPDGE 691

  Fly   733 KG--DKGSFGRSGEKGEPGSCALDEIKMP-AKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNT 794
            .|  :.|..|..|.||..|        .| .||:.|.||:.|.||.|||.|.||.:|...: |..
Mouse   692 PGIPEAGCPGPPGPKGNQG--------FPGTKGSPGCPGEMGKPGRPGEPGIPGAKGEPSV-GRP 747

  Fly   795 GPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQ---PGPRGEPGISRPGPM 856
            |..|.||..|.||..|..|:.|..|..|:||.||:.||.|.||..||   ||.:|.||...|||.
Mouse   748 GKPGKPGFPGERGNAGENGDIGLPGLPGLPGTPGRGGLDGPPGDPGQPGSPGAKGSPGRCIPGPR 812

  Fly   857 GP---PGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGP 918
            |.   ||||||:|:.|.||.|||               :||.|:||:. |.|:.|:
Mouse   813 GTQGLPGLNGLKGQPGRRGDTGP---------------KGDPGIPGMD-RSGVPGD--------- 852

  Fly   919 AGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGD---RGAPGNDGPKGFAGVTGAPGKRG 980
               .||||.||..|..|..|.||.||:||..|.||.||:   .|.||..||.|..|..|:.|:||
Mouse   853 ---PGPPGTPGCPGEMGPPGQKGYPGAPGFPGPPGEKGEVGMMGYPGTTGPPGLPGKPGSQGQRG 914

  Fly   981 PAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQ 1045
            ..||||:.|.||..||.|..|.      :|.||......:|||:   |.||.:|..|.||||||:
Mouse   915 SLGIPGMKGEKGRPGAKGERGE------KGKPGPSQTTLLKGDK---GEPGLKGFVGNPGEKGNR 970

  Fly  1046 GFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPG-LAVHGRAGPPGEKGD 1109
            |.|||.||.||      :|..|.|||.|.|||||..|.||.||..|:|| :.:.|..||.|.||.
Mouse   971 GNPGLPGPKGL------EGLPGLPGPPGPRGDTGSRGNPGRPGPHGMPGSMGIMGVPGPKGRKGT 1029

  Fly  1110 QGRSGIDGRDGINGEKGEQGLQGVWG-----QPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGY 1169
            .|..|:.||.|:.|..|.||.:|..|     :||..|..|.||:||..|..|..|..|.||..|.
Mouse  1030 SGLPGLAGRPGLTGIHGPQGDKGEPGYSEGARPGPPGPKGDPGLPGDKGKKGERGVPGPPGQSGP 1094

  Fly  1170 PGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGER 1234
            .|..|..|.||..|.||..|..|.:||:|..|.|||.|..|..|.||||. :|...|.:|.||..
Mouse  1095 AGPDGAPGSPGSPGHPGKPGPAGDLGLKGQKGFPGPPGSTGPPGPPGLPG-LPGPMGMRGDQGRD 1158

  Fly  1235 GYTGEKGEQGERGL-------TGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPG 1292
            |..|..||:||.||       .|..||.|||||||:   ||.|||.|..|..||:||:|.   ||
Mouse  1159 GIPGPPGEKGETGLLGAYPGPKGSPGVPGAKGDRGV---PGLSGLPGRKGVMGDVGPQGP---PG 1217

  Fly  1293 VTIKGEKGLPGRPGRNGRQGLIGA--PGLIGERGLPGLAGEPGLVGLPGPIGPAGS--KGERGLA 1353
            ..     ||||.|      ||.||  ||..|:||||||.|.||..|.|||.||.|.  ||::|..
Mouse  1218 TA-----GLPGPP------GLPGAIIPGPKGDRGLPGLRGNPGEPGPPGPPGPIGKGIKGDKGFM 1271

  Fly  1354 GSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGY 1418
            |.||.      .|.||..||.||.||.|..|..|..|.:||.|.      .|.||:.|:||:.|:
Mouse  1272 GPPGP------KGLPGTVGDMGPPGFPGAPGTPGLPGVRGDPGF------PGFPGIKGEKGNPGF 1324

  Fly  1419 PGLNGNDGPVGAPGERGFTGPKGRDGR------DGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNG 1477
            .|..|:.|||   |.:|..||:|:.|.      .|:||.||..|:|||...|||.|.||.||..|
Mouse  1325 LGPIGHPGPV---GPKGPPGPRGKPGTLKVISLPGSPGPPGVPGQPGMKGDPGPLGLPGIPGPCG 1386

  Fly  1478 PKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEP 1542
            |:|:||:.|:.|..|..|.:|.||.:|..|..|..|.||.||:   ||:||
Mouse  1387 PRGKPGKDGKPGTPGPAGTKGNKGLKGQQGPPGLDGLPGLKGN---PGDRG-------------- 1434

  Fly  1543 GAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRF 1607
               .||....:.|.:.|||||:..:|:|..|...|::|:|||:|.||..||.||||:.|||:.||
Mouse  1435 ---TPATGTRMRGFIFTRHSQTTAIPSCPEGTQPLYSGFSLLFVQGNKRAHGQDLGTLGSCLQRF 1496

  Fly  1608 STLPVLSCGQNNVCNYASRNDKTFWLTTNAAIP--MMPVENIEIRQYISRCVVCEAPANVIAVHS 1670
            :|:|.|.|..|||||:|||||.::||:|.|.:|  |.|:....:..|||||.|||.||..|||||
Mouse  1497 TTMPFLFCNINNVCNFASRNDYSYWLSTPALMPMDMAPISGRALEPYISRCTVCEGPAMAIAVHS 1561

  Fly  1671 QTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGAKGTCHFYE 1735
            ||..:|.||..|..||.|:||:|.|:.|:.|.||||.|||||||:|||:|||||:| :|||::|.
Mouse  1562 QTTAIPPCPQDWVSLWKGFSFIMFTSAGSEGAGQALASPGSCLEEFRASPFIECHG-RGTCNYYS 1625

  Fly  1736 TMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMK 1776
            ...|||:.:|...:.|.:|...|:|||:.:..:||||||||
Mouse  1626 NSYSFWLASLNPERMFRKPIPSTVKAGDLEKIISRCQVCMK 1666

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	30/56 (54%)
		Collagen	322..380	CDD:189968	28/60 (47%)
		Collagen	413..465	CDD:189968	22/51 (43%)
		Collagen	499..561	CDD:189968	33/70 (47%)
		Collagen	574..632	CDD:189968	29/67 (43%)
		Collagen	657..714	CDD:189968	30/60 (50%)
		Collagen	765..824	CDD:189968	26/58 (45%)
		Collagen	854..911	CDD:189968	24/59 (41%)
		Collagen	884..943	CDD:189968	19/58 (33%)
		Collagen	923..982	CDD:189968	31/61 (51%)
		Collagen	1028..1085	CDD:189968	31/56 (55%)
		Collagen	1229..1287	CDD:189968	32/64 (50%)
		Collagen	1318..1376	CDD:189968	29/59 (49%)
		Collagen	1399..1458	CDD:189968	24/64 (38%)
		Collagen	1477..1534	CDD:189968	24/56 (43%)
		C4	1555..1662	CDD:128421	57/108 (53%)
		C4	1663..1777	CDD:128421	63/114 (55%)
Col4a3	NP_031760.2	7S domain. /evidence=ECO:0000250\|UniProtKB:Q01955	29..42		4/11 (36%)
		Collagen	42..94	CDD:189968	23/51 (45%)
		Triple-helical region	43..1436		667/1550 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	44..473		200/462 (43%)
		Collagen	288..343	CDD:189968	29/66 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	500..1439		460/1062 (43%)
		Collagen	712..765	CDD:189968	25/53 (47%)
		Cell attachment site. /evidence=ECO:0000255	830..832		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	994..996		1/1 (100%)
		Collagen	998..1055	CDD:189968	25/56 (45%)
		Cell attachment site. /evidence=ECO:0000255	1152..1154		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1304..1306		0/1 (0%)
		Collagen	1377..1436	CDD:189968	29/78 (37%)
		Epitope recognized by Goodpasture antibodies. /evidence=ECO:0000250\|UniProtKB:Q01955	1425..1443		9/37 (24%)
		C4	1445..1551	CDD:279721	54/105 (51%)
		Required for the anti-angiogenic activity of tumstatin. /evidence=ECO:0000250\|UniProtKB:Q01955	1478..1556		43/77 (56%)
		C4	1555..1665	CDD:279721	59/110 (54%)
		Required for the anti-tumor cell activity of tumstatin. /evidence=ECO:0000250\|UniProtKB:Q01955	1609..1627		11/18 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	164	1.000	Domainoid score	I3922
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1346	1.000	Inparanoid score	I143
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1239
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	10	9.970