DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and Col2a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_112440.2	Gene:	Col2a1 / 12824	MGIID:	88452	Length:	1487	Species:	Mus musculus

Alignment Length:	1727	Identity:	632/1727 - (36%)
Similarity:	751/1727 - (43%)	Gaps:	411/1727 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 LLYAAVIAGALVGADAQFWKTAGTAGSIQDSVKHYNRNEPKFPIDDSYDIVDSAGVARGDL---P 69
            ||.|||:  ...|.|||      .|||...:.:.|...:...|......:.|:..|...|:   .
Mouse    15 LLIAAVL--RCQGQDAQ------EAGSCLQNGQRYKDKDVWKPSSCRICVCDTGNVLCDDIICED 71

  Fly    70 PK--NCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGEMG----FPGMEGPSGDKGQKGDPGPYG 128
            |.  |....:..|.|.|.|:.....  |.|||.|.|||.|    ..|..||.|.:|..|:.||.|
Mouse    72 PDCLNPEIPFGECCPICPADLATAS--GKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRG 134

  Fly   129 QRGDKGERGSP---GLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYA 190
            .||||||:|:|   |..|:.|.||..||||.||.|            |.|||..       ..:|
Mouse   135 DRGDKGEKGAPGPRGRDGEPGTPGNPGPAGPPGPP------------GPPGLSA-------GNFA 180

  Fly   191 GQL-GSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHG 254
            .|: |...||.                    |.|.:...||..|..|.||..||.||.||:|..|
Mouse   181 AQMAGGYDEKA--------------------GGAQMGVMQGPMGPMGPRGPPGPAGAPGPQGFQG 225

  Fly   255 LKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEG 319
            ..||          ||.||:.|.                |||||..|..|:||..|..|:||..|
Mouse   226 NPGE----------PGEPGVSGP----------------MGPRGPPGPAGKPGDDGEAGKPGKSG 264

  Fly   320 DTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKP 384
            :.||.|.:|.:|.||.||..|.:|:.|.||..|.||:.|.||:.|..|:||:.|.||..|..|.|
Mouse   265 ERGLPGPQGARGFPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLP 329

  Fly   385 GLLGPPGPPG--GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGN 447
            |..|..||.|  |.||..|.|||.||                  |||.   |..||.|.|
Mouse   330 GERGRTGPAGAAGARGNDGQPGPAGP------------------PGPV---GPAGGPGFP----- 368

  Fly   448 EGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGY-GIQGSKGDAGIPGYPGLKG 511
             |.||.|||.|..|..||:|:.|..|.||.||..|..|.:|.||. ||.|:||.||.||..|..|
Mouse   369 -GAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPG 432

  Fly   512 SKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAG 576
            ..|.|              |.||..||.|..|.||.||.||..|.|||.|.||:.|........|
Mouse   433 FPGPR--------------GPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPG 483

  Fly   577 PKGDKGTSGLPGIPGKDGARGPPGERGYPGER---GHDGINGQTGPPGEKGEDGRTGLPGATGEP 638
            |.|::|..|..|.||..|..|||||||.||.|   |.||:.|..|.|||:|..|.||..||.|:|
Mouse   484 PAGEEGKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDP 548

  Fly   639 GKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGT 703
            |:|               |.||.|||:|:      .|.||..||:|:.      |.|||||.||.
Mouse   549 GRP---------------GEPGLPGARGL------TGRPGDAGPQGKV------GPSGAPGEDGR 586

  Fly   704 PGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPG 768
            ||..|..|        .:|:||..|..|.||..|..|::||||..|:..|       :|..|:.|
Mouse   587 PGPPGPQG--------ARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL-------RGLPGKDG 636

  Fly   769 QTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLR 833
            :||..||||..|..||||..|..|.:|.||.||..||.|..|.:|::|..|..|.||..|..|.|
Mouse   637 ETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPRGER 701

  Fly   834 GIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLP 898
            |.||..|.||.:|     ..||.|.||..|..|.||..||.||.|..|..|..|.||:||.||:.
Mouse   702 GFPGERGSPGAQG-----LQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIA 761

  Fly   899 GVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGN 963
            |..|..|.||||            ||.|.||.||.||..|..|.||.   .|..|.||:.|.|
Mouse   762 GPKGDRGDVGEK------------GPEGAPGKDGGRGLTGPIGPPGP---AGANGEKGEVGPP-- 809

  Fly   964 DGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAG 1028
             ||.|..|..||||:||..|.|                  ||.|..|||||.|..|.|||||.|
Mouse   810 -GPSGSTGARGAPGERGETGPP------------------GPAGFAGPPGADGQPGAKGDQGEA- 854

  Fly  1029 APGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLP 1093
                       |:||:.|.||..||.|.||   .:|..|..||.|.||..||      ||..|.|
Mouse   855 -----------GQKGDAGAPGPQGPSGAPG---PQGPTGVTGPKGARGAQGP------PGATGFP 899

  Fly  1094 GLAVHGRAGPPGEKGDQGRSGID---GRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMD 1155
            |.|  ||.||||..|:.|.:|..   |:||..|.:|:.|..|..|.||.:|..||||..|.||.|
Mouse   900 GAA--GRVGPPGANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDD 962

  Fly  1156 GLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPAT 1220
            |..|..|.||..|..|.|      |:.||||.:||      :||.|.|||.||.|.:|.|
Mouse   963 GPSGLDGPPGPQGLAGQR------GIVGLPGQRGE------RGFPGLPGPSGEPGKQGAP----- 1010

  Fly  1221 VPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPR 1285
                    |:.|:|   |..|..|..|||||||..|.:|..|..||||..|..|:   |||.|..
Mouse  1011 --------GASGDR---GPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDGAAGV---KGDRGET 1061

  Fly  1286 GEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGER 1350
            |.:|.||.        ||.||.                              |||.||.|.:|:|
Mouse  1062 GALGAPGA--------PGPPGS------------------------------PGPAGPTGKQGDR 1088

  Fly  1351 GLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGD 1415
            |.||:.|..|..|..||.|:.|..||:|.|||   :|.:|::|.||.||..|..||||..|..||
Mouse  1089 GEAGAQGPMGPSGPAGARGIAGPQGPRGDKGE---SGEQGERGLKGHRGFTGLQGLPGPPGPSGD 1150

  Fly  1416 TGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKG 1480
                  .|..||.|..|.||..||.|..|:||:.|:||..|      ||||:|..|:.|..||.|
Mouse  1151 ------QGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIG------PPGPRGRSGETGPVGPPG 1203

  Fly  1481 EPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPG--ERGYEGAIGLIGQKGEPG 1543
            .||.||.                           |||.|    ||  ...:.|    :||: |.|
Mouse  1204 SPGPPGP---------------------------PGPPG----PGIDMSAFAG----LGQR-EKG 1232

  Fly  1544 APAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGSPGSCVPRFS 1608
               |..:.|:         :::...:....|.        :.||....:.|..:.|..|  |..|
Mouse  1233 ---PDPMQYM---------RADEADSTLRQHD--------VEVDATLKSLNNQIESIRS--PDGS 1275

  Fly  1609 TL-PVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEAPANVIAVHSQT 1672
            .. |..:|....:| :.......:|:..|....:..::           |.|........|:...
Mouse  1276 RKNPARTCQDLKLC-HPEWKSGDYWIDPNQGCTLDAMK-----------VFCNMETGETCVYPNP 1328

  Fly  1673 IEVPDCPNGWEG-------LWIGYSFL--MHTAVGNG 1700
            ..||. .|.|..       :|.|.:..  .|.:.|:|
Mouse  1329 ATVPR-KNWWSSKSKEKKHIWFGETMNGGFHFSYGDG 1364

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	31/63 (49%)
		Collagen	322..380	CDD:189968	27/57 (47%)
		Collagen	413..465	CDD:189968	16/51 (31%)
		Collagen	499..561	CDD:189968	26/61 (43%)
		Collagen	574..632	CDD:189968	31/60 (52%)
		Collagen	657..714	CDD:189968	25/56 (45%)
		Collagen	765..824	CDD:189968	28/58 (48%)
		Collagen	854..911	CDD:189968	29/56 (52%)
		Collagen	884..943	CDD:189968	26/58 (45%)
		Collagen	923..982	CDD:189968	29/58 (50%)
		Collagen	1028..1085	CDD:189968	20/56 (36%)
		Collagen	1229..1287	CDD:189968	26/57 (46%)
		Collagen	1318..1376	CDD:189968	18/57 (32%)
		Collagen	1399..1458	CDD:189968	26/58 (45%)
		Collagen	1477..1534	CDD:189968	14/58 (24%)
		C4	1555..1662	CDD:128421	15/107 (14%)
		C4	1663..1777	CDD:128421	10/47 (21%)
Col2a1	NP_112440.2	VWC	34..88	CDD:278520	9/53 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	96..179		42/103 (41%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	191..1237		536/1361 (39%)
		Triple-helical region	201..1214		523/1296 (40%)
		Collagen	258..317	CDD:189968	28/58 (48%)
		Collagen	375..425	CDD:189968	25/49 (51%)
		Collagen	468..541	CDD:189968	34/72 (47%)
		Collagen	546..617	CDD:189968	38/105 (36%)
		Collagen	684..732	CDD:189968	22/52 (42%)
		Collagen	801..860	CDD:189968	36/91 (40%)
		Collagen	834..893	CDD:189968	34/79 (43%)
		Collagen	933..992	CDD:189968	30/70 (43%)
		Collagen	969..1046	CDD:189968	41/104 (39%)
		Nonhelical region (C-terminal)	1215..1241		10/46 (22%)
		COLFI	1254..1486	CDD:279718	24/126 (19%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810