Protein Alignment Col4a1 and col4a2

DIOPT Version :10

• Sequence 1: NP_723044.1
• Gene: Col4a1 / 33727
• FlyBaseID: FBgn0000299
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_002933063.2
• Gene: col4a2 / 100486079
• XenbaseID: XB-GENE-5950101
• Length: 1722
• Species: Xenopus tropicalis
• Alignment Length: 1871
• Identity: 773/1871 - (41%)
• Similarity: 966/1871 - (51%)
• Gaps: 342/1871 - (18%)

  Fly    52 DDSYDIVDSAGVARGDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPLGPTGLKGE---MGFPGMEG 113
            |.||         .|....::|:   .||  :|:.|||:||.|||||..|..|.   :|..|::|
 Frog    48 DKSY---------TGPCGGRDCS---QGC--QCLPEKGSRGQPGPLGGQGTSGPPGLIGIAGLQG 98

  Fly   114 PSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLE 178
            ..||||::|.||..|..||||:.|..|..|..||||..|..|..|.|   |.|||:|..|..|:.
 Frog    99 RKGDKGERGFPGVTGPSGDKGQSGVTGFPGADGVPGHTGQGGPRGKP---GHDGCNGTQGDAGVG 160

  Fly   179 GLSGMPGPRGYAGQLGSKGEKGEP---AKENGDYAKGEKGEPGWR---------GTAGLAGPQGF 231
            |..|..||.|.:|..|:||:||||   :.|..:..:|:.||.|:.         ||||..||||:
 Frog   161 GSHGSRGPPGISGGFGAKGQKGEPFHVSIEVKNRLRGDPGEAGFNGIQGPRGSPGTAGFIGPQGY 225

  Fly   232 PGEKGERGDSGPYGAKGPRGEHGL--KGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTV- 293
               :|.||..||.|.:||:|..||  .||||.    |..||.||.:..:||.      :..|.:
 Frog   226 ---RGPRGPPGPPGPQGPQGNRGLGYYGEKGE----PGDPGPPGEQPRQGER------EQKHKIE 277

  Fly   294 ------MGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTG 352
                  .|.:||.|:||.|   |.||:|      .:.|:|||:|:.|.||.||..||   .|::|
 Frog   278 ILLLQYKGAKGDQGEKGNP---GDKGQP------AVTGEKGEEGITGFPGQRGFPGN---DGNSG 330

  Fly   353 QKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGP 417
            ..|:.|.||.||:||.||.||:.|..|.      |||.||.   ....|....||..|.:|.||.
 Frog   331 ASGEIGFPGANGVPGLPGTKGKKGEVGD------LGPQGPV---TFVNGQQKRKGYEGEIGFPGL 386

  Fly   418 QGLNGVDGLPGPQGYNGQKGGAGLPGR-PGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPE 481
            .|:.|.||.||..|:.||.|.   |.| ||..|..|.||::|..|..|..|.  |...|||||.:
 Frog   387 NGVYGDDGDPGDPGFPGQNGS---PSRFPGQTGDTGLKGQRGPKGYQGDTGL--PAFQPGPPGID 446

  Fly   482 ---GQKGDAGLPGY-----------GIQGSKGDAGIPGYPGLKGSKGERG----------FKGNA 522
               |::|..|.|||           |..|..|..|.||..||:|.|||:|          .:|.|
 Frog   447 GAPGKEGPPGQPGYPGGRIGMKGLPGFPGRDGQRGAPGSRGLRGIKGEQGECRCYEGDEAGRGPA 511

  Fly   523 GAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGI---------KGDVGGKCSSCRAGPK 578
            |.||  .:|.||..|.||..|:.||.|..|.||..|.:|:         .|:.|.|..:...|.|
 Frog   512 GEPG--PVGFPGFQGHAGRKGEAGDRGVQGLPGAPGTVGLAGPAGFPGPAGEKGDKVFATEKGSK 574

  Fly   579 GDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPAL 643
            |.:|.||.||:||:|         ||||..|.||..|..||.|    ||..||||:.|.||.|..
 Frog   575 GAQGDSGFPGVPGRD---------GYPGRNGRDGSPGFPGPSG----DGIKGLPGSHGFPGLPGN 626

  Fly   644 CDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGN-------- 700
            ..      .:|:.|:||       .||.|::||.|.||.:|..||.|..||||..|:
 Frog   627 AG------ARGETGFPG-------PGFPGSKGLRGEPGNRGVSGFPGVPGLSGPQGDCYQETDEE 678

  Fly   701 -----DGT-------PGRAGRDGYPGIPG-QSIKGEPGF---HGRDGA---KGDKGSFGRSGEKG 746
                 :||       |..||..|.||:|| ..|:|:.||   ||.||.   ||.||..|.....|
 Frog   679 NEIGKEGTGCRLIAPPAPAGAPGPPGLPGYNGIRGQKGFPGPHGFDGVFGLKGIKGDRGTDSLPG 743

  Fly   747 EPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQ--GPPGVEGPRGLN 809
            .||...       .:|::|..|..|:||.||..|.||.:|..|.||:.|.:  .|.|.:|..||.
 Frog   744 PPGFSG-------QRGDRGNAGLPGLPGVPGLSGKPGFKGVQGEKGSVGDRLGAPSGEQGNTGLP 801

  Fly   810 GPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPT 874
            |.:|.||.||..|:||..||.|..|.||..|..|..|.||:  ||..||..|
 Frog   802 GLQGLKGTQGDQGIPGLQGKVGTPGTPGNKGIKGSPGAPGV--PGIPGPAAL------------- 851

  Fly   875 GPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGA 939
              .||||..|:.|.||.:|..|.||..|:.|..||||..||.|..|:.|..||.|..|||||.|.
 Frog   852 --FGFPGMPGNPGTPGPQGSMGPPGPPGQRGDEGEKGPPGPAGMKGLPGELGVGGFIGVRGRHGN 914

  Fly   940 KGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDG 1004
            .|.||..|:.|:||.||.:|:||.:|.:|..|..|.|..||..|..|..|..|.||..|.:|..|
 Frog   915 PGIPGLVGMHGLPGVKGIKGSPGMEGFQGMKGAKGRPAWRGLKGPSGNIGFPGVKGGNGTSGTKG 979

  Fly  1005 PVGGRGPPGAP-----------------------GLMGIKGDQGLAGAPGQQGLDGMP------- 1039
            ..|.:||||.|                       |:.|:||.:|:.|.|||.||.|:|
 Frog   980 DRGEQGPPGDPPKMPEMMLLTKGETGDQGVSGFKGISGLKGSKGMPGPPGQLGLPGLPGLRSFEF 1044

  Fly  1040 GEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGP- 1103
            |:||..|.||:.|..|:.|:....|..|.||.:|.||..|.:|.||.|||:|..|..  |..|.
 Frog  1045 GDKGETGTPGVIGNQGILGEVGPDGIIGFPGFTGPRGTPGISGFPGVPGERGKYGDI--GERGDT 1107

  Fly  1104 ---PGEKGDQGRS---GIDGRDGINGEK---GEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPG 1159
               |||:|.:|:.   |:.|:.||:|||   |::|.:|:.|..||.|..|..|.||..|:.|.||
 Frog  1108 IDLPGERGTKGQGGTPGLPGQRGIHGEKGTTGDEGFRGIEGVIGEHGQTGEKGFPGQQGLVGFPG 1172

  Fly  1160 AAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDI 1224
            :.|.||..|.||::|.      ||.|||.|:.|..|::|..|..|..|.:||.||||     .||
 Frog  1173 SQGFPGLPGVPGEKGS------SGFPGLPGQHGFPGIRGIAGLDGLPGTKGINGQPG-----ADI 1226

  Fly  1225 RGDKGSQGERGYTGEKGEQGER----GLTGPAGVAGAKGDRGLQ---GPPGASGLNGIPGAKGDI 1282
            .|.||.   :|.:|.||:.||.    |:.||||..||:||:||.   |.||..|:.|:||.....
 Frog  1227 IGLKGF---KGLSGGKGQPGEASTIVGMPGPAGSKGAEGDQGLPGLIGLPGTVGVRGLPGFSNKT 1288

  Fly  1283 GPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSK 1347
            |..|::|.|     |..||||.||..||               |||..:||:.|:.|.:|..|:.
 Frog  1289 GLLGDVGPP-----GPPGLPGFPGPIGR---------------PGLPSKPGVKGVIGDLGLQGNY 1333

  Fly  1348 GERGLAGSPGQ---PGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQK---GDKGDRGLQGPSGL 1406
            |.:|:.|..|:   ||..|.|||.||||..|.|||.|..|..|.:|.|   ||.|:.||:||.|.
 Frog  1334 GAKGIPGDEGKVGVPGLAGIPGAKGLKGSAGFQGFVGVSGYRGDQGPKGLRGDIGEYGLKGPPGQ 1398

  Fly  1407 PGLVGQ----KGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPK 1467
            ||.:.:    ..:.||||.:   |.:|..|.:|..|||   |..||||.|||.|:.|:
 Frog  1399 PGPMSEPPLITAEQGYPGAH---GIIGHQGVQGDMGPK---GLVGTPGAPGQTGKEGL------- 1450

  Fly  1468 GEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGA 1532
              ||.|      |.|..||.|                  ||.|.:||.||         .|::||
 Frog  1451 --PGVP------GWPAPPGSR------------------GEQGPMGRTGP---------NGFKGA 1480

  Fly  1533 IGLIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDL 1597
            .|:.|:.|.||.|..:.   ..|.|:.:|||::..|.|..|...|||||||||.:|.:.||||||
 Frog  1481 SGVPGRAGLPGMPGRSV---NIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDL 1542

  Fly  1598 GSPGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIPMMPVENIEIRQYISRCVVCEAP 1662
            |..|||:.||||:|.|.|...:||.||:||||::||:|.|.:|||||...|||.|||||.|||||
 Frog  1543 GLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAP 1607

  Fly  1663 ANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGA 1727
            |..||||||.:.:|.||:||..||||||||||||.|:.||||:|.|||||||||||||||||||.
 Frog  1608 AVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGG 1672

  Fly  1728 KGTCHFYETMTSFWMYNLESSQPFE-RPQQQTIKAGERQSHVSRCQVCMKN 1777
            :||||::....|||:..::  :||: .|...|:|||..::|:|||||||||
 Frog  1673 RGTCHYFANKYSFWLTTID--EPFQSSPPADTLKAGLIRTHISRCQVCMKN 1721

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	gly_rich_SclB	<107..>361	CDD:468478	109/274 (40%)
		gly_rich_SclB	<355..>642	CDD:468478	127/320 (40%)
		gly_rich_SclB	<543..820	CDD:468478	117/314 (37%)
		gly_rich_SclB	<727..>968	CDD:468478	103/245 (42%)
		gly_rich_SclB	<969..>1218	CDD:468478	112/288 (39%)
		gly_rich_SclB	<1186..>1420	CDD:468478	101/250 (40%)
		gly_rich_SclB	<1321..>1547	CDD:468478	87/235 (37%)
		C4	1555..1662	CDD:128421	63/106 (59%)
		C4	1663..1777	CDD:128421	71/114 (62%)
col4a2	XP_002933063.2	gly_rich_SclB	<95..>363	CDD:468478	123/301 (41%)
		gly_rich_SclB	<285..>515	CDD:468478	101/255 (40%)
		gly_rich_SclB	<502..>772	CDD:468478	111/304 (37%)
		gly_rich_SclB	<803..1050	CDD:468478	106/263 (40%)
		gly_rich_SclB	<965..>1223	CDD:468478	102/265 (38%)
		gly_rich_SclB	<1112..>1355	CDD:468478	109/276 (39%)
		gly_rich_SclB	<1312..>1495	CDD:468478	87/230 (38%)
		C4	1501..1606	CDD:460201	61/104 (59%)
		C4	1611..1720	CDD:460201	69/110 (63%)