DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col5a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_021324673.1	Gene:	col5a1 / 100334431	ZFINID:	ZDB-GENE-041105-6	Length:	2024	Species:	Danio rerio

Alignment Length:	1542	Identity:	585/1542 - (37%)
Similarity:	707/1542 - (45%)	Gaps:	410/1542 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    15 AGALVGADAQFWKTAGTAG------SI------QDSVKHYN--RNEPKFPIDDSYDIVDSAGVAR 65
            |||..||::.  .|.|..|      ||      ::|:..|.  ..|..:..||.|...|.|
Zfish   559 AGAAAGAESV--ATGGQGGIGGEAESIDIDKFKEESITDYTDAELEKMYDYDDLYTDGDKA---- 617

  Fly    66 GDLPPKNCTAGYAGCVPKCIAEKGNRGLPGPL-------GPTGLKGEMGFPGMEGPSGDKGQKGD 123
              ||.:  |....|.|.....|||.:|.|..:       ||:|.:|..|.||..|.:|..|..|:
Zfish   618 --LPAE--TDEIYGQVDGLRGEKGQKGEPAIIEPGMLVEGPSGPEGPTGLPGPPGSTGPPGSAGE 678

  Fly   124 PGPYGQRGDKGERGSPGLHGQAGVPGV----------------QGPA------------------ 154
            |   |:||..|..|.||..|..|.||.                :|||
Zfish   679 P---GERGLPGRAGLPGADGLPGPPGTVLMLPFRMSTGGDGGQKGPAVSAQEAQMQAIMQQARLA 740

  Fly   155 --GNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKE---NGDYAKGEK 214
              |..|..|:.|:.|..|..|:.||:|.||..||:|..|.:||.|..|:|.:.   ..|.|:|..
Zfish   741 MRGPTGPMGLTGRPGPLGPPGVSGLKGESGESGPQGPRGPMGSAGPTGKPGRRGRPGSDGARGMP 805

  Fly   215 GEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKG 279
            |:.|.:|..|..|..|.|||||.||::||.|..||.||.|.:|:.|.  .||.  |.||..|.:|
Zfish   806 GQTGPKGDRGFDGLAGLPGEKGHRGETGPQGPPGPSGEDGERGDDGE--VGPR--GLPGEPGPRG 866

  Fly   280 EPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGN 344
                         |:||:|..|..|.||:.|..|.|||:|:.|..|:.|..|..|.||.:|..|.
Zfish   867 -------------VLGPKGPQGPPGPPGVTGMDGHPGPKGNIGPQGEPGPPGQQGNPGAQGLNGP 918

  Fly   345 FGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPR 409
            .||.|..|:||..|:|||.|:||..|..|.||:.|             |.|.:|..||.||:||.
Zfish   919 QGPIGPPGEKGPTGKPGLPGMPGADGPPGHPGKEG-------------PSGEKGNLGPHGPQGPI 970

  Fly   410 GYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTA------GLNGPKGS 468
            ||   |||:|:.|.||:.|.:|..|:||..|.||..|:.|..|.|||.|.|      |..||||.
Zfish   971 GY---PGPRGVKGADGVRGLKGNKGEKGEDGFPGFKGDMGVKGDKGELGAAGPRGEDGPEGPKGR 1032

  Fly   469 IGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRP 533
            .|..|.|||.||.|:||..|:|           |:|||||.:|.||.:||:|..|..|:.
Zfish  1033 SGLPGDPGPLGPLGEKGKLGVP-----------GLPGYPGRQGPKGSQGFQGFQGTSGEK----- 1081

  Fly   534 GTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGP 598
            ||.|.||.||.:|..|..|..|::|..|..|         :|||||:.|:.|.||.||:.|..||
Zfish  1082 GTRGTAGKPGPRGQRGPTGPRGERGPRGPTG---------KAGPKGNSGSDGPPGPPGERGLLGP 1137

  Fly   599 PGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPG 663
            .|..|:||.:         ||||..|:||..|.||..||.|            .:|..|.||.||
Zfish  1138 QGPAGFPGPK---------GPPGPPGKDGLPGHPGQRGETG------------FQGKTGPPGPPG 1181

  Fly   664 AKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHG 728
            ..|.|            ||.||.|..||:               |..|.||.||:  :|.||..|
Zfish  1182 VVGPQ------------GPTGETGPMGER---------------GHPGPPGPPGE--QGLPGAAG 1217

  Fly   729 RDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGN 793
            ::|||||.|..|.||:.|.||.             :|.||:.|:|||.|.         .|||||
Zfish  1218 KEGAKGDPGPAGPSGKDGPPGL-------------RGFPGERGLPGPVGS---------AGLKGN 1260

  Fly   794 TGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGP 858
            .||.|||   ||.|..|.||..|..|..|:||                          ||||.||
Zfish  1261 EGPPGPP---GPAGSPGERGPAGPAGPTGIPG--------------------------RPGPQGP 1296

  Fly   859 PGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAG 923
            ||..|.:|..|::||.||.|..|..|.||.|                           ||||..|
Zfish  1297 PGPAGEKGGPGEKGPQGPAGRDGIQGPVGLP---------------------------GPAGPIG 1334

  Fly   924 PPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFA---GVTGAPGKRGPAGIP 985
            ||         |.||.|||.|.||..|..|:||::|.||..||:|.|   |.:||.|:.||.|..
Zfish  1335 PP---------GEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPAGQPGPSGADGEPGPRGQQ 1390

  Fly   986 GVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGL 1050
            |:.|.|||:|:.|..|..||||.:|.||.||..|..||.|..|.||.      ||.:|..|.||.
Zfish  1391 GLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGP------PGPRGPSGPPGA 1449

  Fly  1051 DGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGI 1115
            |||.|.||..      |.||..|.:||.|.||.||..|:.|.|        ||.||:|::|.:|.
Zfish  1450 DGPQGPPGGI------GNPGAVGEKGDAGEAGEPGPQGDIGPP--------GPRGERGEKGEAGP 1500

  Fly  1116 DGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPG 1180
            .|..|..|.||..|..|..|.||..|..|.||.||.||..||.|:         |||:||.||||
Zfish  1501 AGSGGPPGPKGPPGDDGPKGSPGPSGFPGDPGPPGEPGPSGLDGS---------PGDKGDDGEPG 1556

  Fly  1181 LSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGE 1245
            ..|.||..|||||         |||.|:||..|    ||                  |.:|.|||
Zfish  1557 QPGSPGPTGETGP---------PGPPGKRGPHG----PA------------------GPEGRQGE 1590

  Fly  1246 RGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGR 1310
            :         ||||:.||:||||.:         |.:||:|.                 ||:.|.
Zfish  1591 K---------GAKGESGLEGPPGKT---------GPVGPQGS-----------------PGKPGP 1620

  Fly  1311 QGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTG 1375
            :||.|.||.:||:|||   |.||..|.|||:||                     ||.||||||.|
Zfish  1621 EGLRGVPGPVGEQGLP---GAPGPDGPPGPMGP---------------------PGLPGLKGDLG 1661

  Fly  1376 PQGFKGERGLNGF---EGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFT 1437
            .:|.||..||.|.   .|::|:||||||.||.|..   |.|||.|..|.:|..||:|.||..|..
Zfish  1662 IKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGTS---GPKGDNGIAGPSGPIGPIGPPGLPGPP 1723

  Fly  1438 GPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPG-----QPGRNGPK 1479
            ||||..|..|..|..|:.|.||   ||||.|.||     .|.::.||
Zfish  1724 GPKGAKGSSGQTGPKGESGIPG---PPGPPGPPGDVIHPMPYQSSPK 1767

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	25/92 (27%)
		Collagen	322..380	CDD:189968	26/57 (46%)
		Collagen	413..465	CDD:189968	24/57 (42%)
		Collagen	499..561	CDD:189968	26/61 (43%)
		Collagen	574..632	CDD:189968	26/57 (46%)
		Collagen	657..714	CDD:189968	16/56 (29%)
		Collagen	765..824	CDD:189968	27/58 (47%)
		Collagen	854..911	CDD:189968	19/56 (34%)
		Collagen	884..943	CDD:189968	16/58 (28%)
		Collagen	923..982	CDD:189968	28/61 (46%)
		Collagen	1028..1085	CDD:189968	24/56 (43%)
		Collagen	1229..1287	CDD:189968	18/57 (32%)
		Collagen	1318..1376	CDD:189968	23/57 (40%)
		Collagen	1399..1458	CDD:189968	27/58 (47%)
		Collagen	1477..1534	CDD:189968	2/3 (67%)
		C4	1555..1662	CDD:128421
		C4	1663..1777	CDD:128421
col5a1	XP_021324673.1	TSPN	16..207	CDD:214560
		Med15	637..>974	CDD:312941	135/372 (36%)
		Collagen	776..831	CDD:189968	22/54 (41%)
		Collagen	929..986	CDD:189968	31/72 (43%)
		PHA03169	<941..>1115	CDD:223003	85/214 (40%)
		Collagen	962..1021	CDD:189968	31/61 (51%)
		Collagen	1589..>1632	CDD:189968	24/77 (31%)
		COLFI	1796..2022	CDD:307527
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810