DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col5a3a

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_001177685.1	Gene:	col5a3a / 100330523	ZFINID:	ZDB-GENE-030131-968	Length:	1887	Species:	Danio rerio

Alignment Length:	1733	Identity:	611/1733 - (35%)
Similarity:	743/1733 - (42%)	Gaps:	466/1733 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    88 KGNRGLP----------GPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLH 142
            ||.:|.|          ||.||.|.:|..|.||:.||         |||.|..||.|..|.|||.
Zfish   486 KGQKGEPAVVEPGAVFQGPPGPPGPQGLPGIPGITGP---------PGPSGDPGDVGPEGRPGLP 541

  Fly   143 GQAGVPGVQGPAGNPGA----PGINGKDGCDGQ--------------------DGIPGLEGLSGM 183
            |..|:|      |.||.    |...|.|...|.                    .|.||..||:|.
Zfish   542 GADGIP------GPPGTMLMLPFHYGGDSQKGPMISAQQAQAQAILQQTKLSLKGPPGPLGLTGR 600

  Fly   184 PGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKG 248
            |||.|..|..|.||:            .||.|.||.||..|.|||.|.|   |:||.||..|.:|
Zfish   601 PGPVGLPGPSGLKGD------------IGENGPPGPRGLPGSAGPNGKP---GKRGRSGADGGRG 650

  Fly   249 PRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKG 313
            ..||.|.||::|..       |.||:.|.||.                |||   :|:||..|..|
Zfish   651 APGETGAKGDRGFD-------GLPGLPGNKGH----------------RGD---RGKPGPQGPHG 689

  Fly   314 EPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRA 378
            |||.:|.   ||..|.:|.||.||.||..|..||||..||:|.:|..|:.|..||.|..||||..
Zfish   690 EPGHKGS---DGPVGPRGQPGEPGIRGLVGPRGPPGPPGQQGIQGVDGVQGPKGNLGPLGEPGPP 751

  Fly   379 GATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPG 443
            |..|.||:.|.|||    :|..|.||.|||.   |.||.|||.|:|   ||            ||
Zfish   752 GQQGNPGIQGFPGP----QGPIGLPGEKGPE---GKPGMQGLPGID---GP------------PG 794

  Fly   444 RPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPG 508
            .||.|||||:||.:|.:|..      ||:|:|||.|.:|..|..||     :||||:.|..|:||
Zfish   795 HPGREGPPGEKGHQGLSGAQ------GPVGYPGPRGVKGADGVRGL-----KGSKGEKGEDGFPG 848

  Fly   509 LKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSC 573
            .||..|.:|.:|:.|:||..     |..|..|..||.|..|.||:
Zfish   849 AKGEMGIKGDRGDNGSPGSR-----GEDGPEGPKGQSGPLGEPGS-------------------- 888

  Fly   574 RAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEP 638
             ||..|:||..|:||:||..|.:|..|..|:||..|.:|..|:.||.|:.|.||..|..||.|..
Zfish   889 -AGIAGEKGKLGVPGLPGYPGRQGSKGSVGFPGTAGLEGEKGKRGPAGQAGADGERGPNGARGGR 952

  Fly   639 GKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGT 703
            |            .:|..|.|||.|:.|..|..||.|..|..||:|..|..|.||.:|.||.||.
Zfish   953 G------------ARGPTGKPGAKGSAGHDGPPGATGERGPQGPQGRNGEAGPKGPNGPPGKDGL 1005

  Fly   704 PGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPG 768
            ||.         |||  :|||||.|:.|..|..|..|..|:.||.|          ..|::|.|
Zfish  1006 PGH---------PGQ--RGEPGFQGKTGPPGPAGVVGPQGKTGETG----------PTGDRGHP- 1048

  Fly   769 QTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLR 833
              |.||||||.|.||..|..|.||:.||.|.||..||.|..|.|||:      |:||..|..||:
Zfish  1049 --GAPGPPGEQGLPGAAGKEGTKGDPGPAGQPGKSGPAGRRGFRGER------GLPGILGSSGLK 1105

  Fly   834 GIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLP 898
            |           ||      ||:|..|..|..||:|..||.|.||.||..|.||..|..|:.|.|
Zfish  1106 G-----------GE------GPLGVAGPIGATGERGSSGPAGAIGQPGRQGGVGPAGPMGEKGEP 1153

  Fly   899 GVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGN 963
            |..|..|..|..|:.||:|..|.|||||.|      |.||.|||.|.||..|..|:||:.|.|
Zfish  1154 GEKGSIGPAGRDGEQGPVGLPGPAGPPGPP------GDDGDKGETGGPGQKGSKGDKGEGGPP-- 1210

  Fly   964 DGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAG 1028
                      |.||.:||||.|                        ||||..|..|::|.||:.|
Zfish  1211 ----------GPPGSQGPAGQP------------------------GPPGLDGEPGLRGQQGMYG 1241

  Fly  1029 APGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLP 1093
            ..|.:|..|.   ||..|.|||.|.||.||:..|.|..|..||.|..|..||.|..|..|.:|:|
Zfish  1242 PKGDEGERGF---KGATGPPGLQGMPGPPGEKGESGHVGSMGPPGQHGPRGPQGAIGGEGPQGMP 1303

  Fly  1094 GLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLP 1158
            |..  |:.|..||||:      ||..|..|..||.||.|..|:.||||..|.||..|.||:.|:|
Zfish  1304 GAV--GQPGVVGEKGE------DGEAGNPGNVGETGLVGEKGEVGEKGDAGPPGAAGPPGIRGIP 1360

  Fly  1159 GA---AGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPAT 1220
            |:   .|.||.:|:|||.|..||||::|:.|     || |.:|..|.||..|.:|..|:||.|
Zfish  1361 GSDGPKGNPGPLGFPGDSGPSGEPGVNGIDG-----GP-GAKGDNGEPGKSGPQGASGEPGPP-- 1417

  Fly  1221 VPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPR 1285
                    |..|.||:.|.:|::|::|..|..|.|||:|..|..||               :||:
Zfish  1418 --------GPPGRRGHIGPEGKEGKQGKKGAKGSAGAEGPIGKTGP---------------VGPQ 1459

  Fly  1286 GEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGER 1350
                             |.||..|.:||.|.||..||:   ||.|.||..|.|||:|||
Zfish  1460 -----------------GHPGNPGPEGLRGIPGSAGEQ---GLNGPPGQTGPPGPMGPA------ 1498

  Fly  1351 GLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGD 1415
                           |.||||||               .|.|||||..||.|..|.||.:|:|||
Zfish  1499 ---------------GLPGLKGD---------------PGIKGDKGHGGLIGLIGPPGEMGEKGD 1533

  Fly  1416 TGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKG 1480
            .|.||:.                           |:.|.||:.|...||||.|.||.||.:|..|
Zfish  1534 RGLPGIQ---------------------------GIQGTKGDGGAGGPPGPTGPPGPPGLSGAIG 1571

  Fly  1481 EPGRPGERGLIGIQGERGEKGERGLIGETGNVGRP-----GPKGDRGEPGERGYEG--------A 1532
            |.|..|::|.||.:|:.|..|..|..|....:..|     |.|..|.....:|.|.        |
Zfish  1572 EKGSKGDQGAIGARGDTGPAGPPGPPGPPAAMIEPLPIREGRKKRRRHSNRQGAESRAEAEEEEA 1636

  Fly  1533 IGLIGQKGEPGAPAPAALDYLTGI--------------------LITRHSQSETVPACSAGHTEL 1577
            :.|   ..|....|..|||...|:                    |.|..|.:.|.......|.:.
Zfish  1637 VQL---NMEDFLQADEALDEPEGMEEVFASLNSMKTEVELMRKPLGTFESPARTCKELMLCHPDY 1698

  Fly  1578 WTGYSLLYVDGNDYAHNQDL-------GSPGSCV---PRFSTLPVLSCGQNNVCNYAS--RNDKT 1630
            ..|.  .::|.|...|...:       ....:|:   .||.|:.:.:..:....::.|  |..|.
Zfish  1699 KDGE--YWIDPNQGCHRDSIRVFCNFTAEGETCLHPDKRFETVKLAAWNKEKPGSWYSQYRKGKQ 1761

  Fly  1631 FWLTTNAAIPMMPVENIEIRQYISRCVVCEAPANVIAVHSQTIEVPDCPN--GWEGLWIGYSFLM 1693
            |........| :||..:...:.:|            |...||... .|.|  ||..:
Zfish  1762 FSYIDTEGNP-VPVVQMTFLKLLS------------ATAMQTFTF-SCQNSIGWYDM-------- 1804

  Fly  1694 HTAVGNGGGGQALQSPGSCLEDFR--ATPFIE-----CNGAKG 1729
                .|.....||:..||..|:..  .:|||.     |...||
Zfish  1805 ----SNHNHQHALRFRGSNDEEMTQDKSPFITALYDGCQTRKG 1843

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	23/60 (38%)
		Collagen	322..380	CDD:189968	28/57 (49%)
		Collagen	413..465	CDD:189968	23/51 (45%)
		Collagen	499..561	CDD:189968	22/61 (36%)
		Collagen	574..632	CDD:189968	26/57 (46%)
		Collagen	657..714	CDD:189968	25/56 (45%)
		Collagen	765..824	CDD:189968	29/58 (50%)
		Collagen	854..911	CDD:189968	26/56 (46%)
		Collagen	884..943	CDD:189968	27/58 (47%)
		Collagen	923..982	CDD:189968	24/58 (41%)
		Collagen	1028..1085	CDD:189968	25/56 (45%)
		Collagen	1229..1287	CDD:189968	19/57 (33%)
		Collagen	1318..1376	CDD:189968	22/57 (39%)
		Collagen	1399..1458	CDD:189968	17/58 (29%)
		Collagen	1477..1534	CDD:189968	20/69 (29%)
		C4	1555..1662	CDD:128421	22/138 (16%)
		C4	1663..1777	CDD:128421	19/76 (25%)
col5a3a	NP_001177685.1	TSPN	30..220	CDD:214560
		Collagen	644..703	CDD:189968	30/87 (34%)
		Collagen	755..813	CDD:189968	36/79 (46%)
		Collagen	791..849	CDD:189968	33/80 (41%)
		Collagen	827..886	CDD:189968	27/68 (40%)
		Collagen	860..917	CDD:189968	26/82 (32%)
		Collagen	893..945	CDD:189968	23/51 (45%)
		Collagen	959..1028	CDD:189968	36/79 (46%)
		Collagen	1022..1080	CDD:189968	29/70 (41%)
		Collagen	1100..1157	CDD:189968	29/73 (40%)
		Collagen	1223..1274	CDD:189968	25/53 (47%)
		Collagen	1256..1314	CDD:189968	26/59 (44%)
		Collagen	1357..1407	CDD:189968	24/55 (44%)
		COLFI	1659..1885	CDD:279718	40/213 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910