DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a1 and col4a1

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_002933064.3	Gene:	col4a1 / 100124870	XenbaseID:	XB-GENE-6451485	Length:	1669	Species:	Xenopus tropicalis

Alignment Length:	1807	Identity:	803/1807 - (44%)
Similarity:	974/1807 - (53%)	Gaps:	259/1807 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    74 TAGYAGC-VPKC-----IAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGD 132
            :|..:|| ..||     ..:||.||||      ||.|.|||||::||      :|.|||.||:||
Frog    21 SAACSGCGTSKCDCSGVKGQKGERGLP------GLPGLMGFPGLQGP------EGPPGPLGQKGD 73

  Fly   133 KGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLS---------GMPGPRG 188
            .||.|.||..|..|.||..|..||||.|||.|:||..|..||||..|..         |:||..|
Frog    74 TGEPGLPGTKGTRGPPGSHGFPGNPGLPGIPGQDGPPGPSGIPGCNGTKGDRGPLGPPGLPGIIG 138

  Fly   189 YAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEH 253
            |.|..|..|.||||....|......|||.|:.|..|:.|..|.||..|..|..||.|:.||.|.|
Frog   139 YPGSPGQPGPKGEPGDITGIDVSMIKGEKGFPGINGIPGTPGLPGTPGPMGPIGPPGSAGPPGSH 203

  Fly   254 GLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKG-EP-G 316
                            ||||.|||.|   .||        .||:|:.|.:|.||...:.. || |
Frog   204 ----------------GAPGPKGEMG---ISF--------YGPKGERGDRGPPGAPCKPSTEPQG 241

  Fly   317 PEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGAT 381
            .|.:....|:|||||:||..|:||:.|.       ..||::||||..|..|.||:.|:.|..|..
Frog   242 SENEQVYYGEKGEKGMPGENGERGQPGQ-------SFKGEKGEPGPLGPRGKPGKDGDQGEKGLQ 299

  Fly   382 GKPGLLGPPGPP--GGGRGTPGPPGPKGPRGYVGAPG-----PQGLNGVDGLPGPQGYNGQKGGA 439
            |.||:.|.||||  ||.:|..|..||:||.|.|.:.|     .:|..|:.|.||.:|..|.||..
Frog   300 GVPGIPGFPGPPGFGGTKGEKGESGPQGPPGIVVSNGEIEVCEKGDRGLPGFPGSKGDKGLKGAQ 364

  Fly   440 GLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIP 504
            |.||.||:.|.|             ..||.||   ||.||..|:||..||||..:||.||..|.|
Frog   365 GFPGIPGSPGVP-------------LPGSTGP---PGFPGERGEKGQHGLPGLALQGPKGRDGFP 413

  Fly   505 GYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGA-----PGQKGDAGRPGTPGQ---KGDMG 561
            |.|||                       ||.|||...     .||.||.|.||.|||   :|:.|
Frog   414 GPPGL-----------------------PGPPGAIDTSIECKSGQPGDPGLPGNPGQNGFQGERG 455

  Fly   562 IKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGER------GYPGERGHDGINGQTGPP 620
            .|||.|..|.||...|         ||:||..|.:||||::      |..|:|||.|.:|..|.|
Frog   456 QKGDKGDACPSCDLVP---------PGLPGPPGPQGPPGDQGASGVPGIKGDRGHPGYDGPPGAP 511

  Fly   621 GEKGEDGRTGLPGATGEPGKPALCDLSLIEP-LKGDK------GYPGAPGAKGVQGFKGAEGLPG 678
            |.:|..|..|.|||.||||:      ...|| |||||      |:|||||.:|..|..|.:||||
Frog   512 GPQGSAGLMGSPGAKGEPGE------VFFEPRLKGDKGESGLPGHPGAPGREGRPGIDGQQGLPG 570

  Fly   679 IPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSG 743
            ..|..|..|.|||:||   ||:.|:||..|..|:||..|....|.||..|..|..|..|..|..|
Frog   571 SKGVAGTIGAKGEQGL---PGDRGSPGNPGERGFPGPAGTGTSGLPGEKGEPGVPGFVGIPGTPG 632

  Fly   744 EKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGL 808
            .|||||...   ||....|..|..|..|.||..||.||||..|..|.:|..|..|.|||    |.
Frog   633 AKGEPGKAI---IKTGPPGPMGGAGIPGFPGDQGEKGSPGHPGRPGSQGEKGEPGLPGV----GY 690

  Fly   809 NGPRGEKGNQGAVGVPGN---PGKDGLRGIPGRNGQPGPRGEPGISRPGP---MGPPGLNGLQGE 867
            .||.||||.||..|.||.   ||..|:.|:||..|.|||:||||:..|||   .||||..|:.|:
Frog   691 PGPSGEKGFQGMPGTPGQAGFPGPQGVDGLPGLRGDPGPKGEPGLGLPGPKGVQGPPGPVGIPGD 755

  Fly   868 KGDRGPTGPIGFPGADGSVGYPGDRGDAGL---PGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPG 929
            ||:.|.:|..|..|..|..|:||.:|:||:   ||:.|.||:.|    :||.|..|.:||.|:.|
Frog   756 KGNPGASGVPGEQGFSGVPGHPGHKGEAGIVGVPGIQGPPGVPG----MGPKGQQGSSGPQGLSG 816

  Fly   930 IDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDK 994
            ..|..|..|.:|..|.||| .|||.|||:|..|:.||.|..|:.|.||..|..|:||..|.||:.
Frog   817 FPGPAGVKGERGYAGFPGL-DMPGPKGDKGNIGSPGPTGATGIPGQPGAPGKEGLPGFPGPKGEM 880

  Fly   995 GATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPG---- 1055
            |..|:.|:   :|..|.||:||.:|.|||.|..|.||..|..|..||||.:   ||.|.||
Frog   881 GVMGMPGS---IGQPGAPGSPGALGEKGDAGPFGPPGTSGYPGTKGEKGEE---GLRGEPGKMSE 939

  Fly  1056 -----LPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGI 1115
                 :.|...|||::||.|.||.:|..|..|:||.||:.|..|  |.|.:||.||||.|   ||
Frog   940 TDKETMKGSKGEKGERGEIGISGEKGSLGDFGSPGMPGKDGQQG--VQGESGPKGEKGSQ---GI 999

  Fly  1116 DGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPG 1180
            .|..|:.|:||..|..|:.|..||||..|..|.||:||:.|:.|..|..|.||.||       .|
Frog  1000 KGSQGLQGQKGSIGEMGLPGPTGEKGQHGISGSPGSPGLGGIKGEKGLKGEVGLPG-------IG 1057

  Fly  1181 LSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGE 1245
            :.|:||:||:.|..|..|..|..|.||:.||.|.||.|.: ....|..|..|..|..||||.:|.
Frog  1058 IPGVPGVKGDAGRDGPHGLPGERGDKGDVGIPGMPGFPGS-KGATGHAGIPGSPGLPGEKGAKGT 1121

  Fly  1246 RGLTGPAGVAGAKGDRGLQGPPGASGLNGIP---GAKGDIGPRGEIGYPGVTIKGEKGLPGRPGR 1307
            .||.|..|..|..||.|:|||||.||..|.|   |..|..|.:||.|.||      :||||.||.
Frog  1122 HGLVGIPGQKGLTGDSGVQGPPGHSGEKGEPGYDGIPGSPGEKGEAGIPG------RGLPGHPGE 1180

  Fly  1308 NGRQGL---IGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGL-----AGSPGQPGQDGF 1364
            :|.:|.   ||.||..|..|:||:.|:.||.|..||.||.|..|..|:     .|..|||||.|:
Frog  1181 HGEKGSKGDIGFPGAPGISGVPGIKGDQGLPGFQGPQGPPGFPGPSGIPMEGPKGEKGQPGQAGY 1245

  Fly  1365 PGAP---GLKGDTGPQGFKGERGLN------GFEGQKGDKGDRGLQGPSGLPGLVGQKGDT---- 1416
            ||:|   ||.|.||..|.|||:|..      ||:|.|||.|::|..|.:|..|:.||||||
Frog  1246 PGSPGPVGLPGHTGQDGLKGEKGERGWPGALGFQGIKGDTGNQGPSGDAGPSGITGQKGDTGPPG 1310

  Fly  1417 --GYPGLNGN---DGPVGAPGERGFTGPKGRDGRDGTPG----LPGQKGEPGMLPPPGPKGEPGQ 1472
              |:|||.|:   .|..|..|::|.|||||..|..|..|    ..|:.||||...|.||:|..|.
Frog  1311 IPGFPGLKGSLGISGEKGDRGDQGITGPKGDLGPPGPAGTHTVTKGESGEPGPQGPSGPRGIQGL 1375

  Fly  1473 PGRNGPK---GEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIG 1534
            ||..|.|   |..|.||..|:.||.|..|:|||.|..|.||..|.|||      ||..|..|.||
Frog  1376 PGPKGDKGVTGASGLPGPSGIPGIHGTPGQKGETGPSGPTGQTGYPGP------PGSDGLPGPIG 1434

  Fly  1535 LIGQKGEPGAPAPAALDYLTGILITRHSQSETVPACSAGHTELWTGYSLLYVDGNDYAHNQDLGS 1599
            |      ||:|:.|     .|.|:|||||:...|.|..|.|.:::|||||||.||:.||.||||:
Frog  1435 L------PGSPSMA-----HGFLVTRHSQTTETPQCPFGTTAVYSGYSLLYVQGNERAHGQDLGA 1488

  Fly  1600 PGSCVPRFSTLPVLSCGQNNVCNYASRNDKTFWLTTNAAIP--MMPVENIEIRQYISRCVVCEAP 1662
            .|||:.:|||:|.|.|..|||||:|||||.::||:|...:|  |.|:....|:.:||||.|||.|
Frog  1489 SGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGDGIKPFISRCTVCEVP 1553

  Fly  1663 ANVIAVHSQTIEVPDCPNGWEGLWIGYSFLMHTAVGNGGGGQALQSPGSCLEDFRATPFIECNGA 1727
            |.|:|||||||::|.||:||..|||||||:|.|:.|..|.||||.|||||||:||:.|||||:|
Frog  1554 AMVMAVHSQTIQIPQCPDGWISLWIGYSFVMQTSAGAEGSGQALASPGSCLEEFRSVPFIECHG- 1617

  Fly  1728 KGTCHFYETMTSFWMYNLESSQPFERPQQQTIKAGERQSHVSRCQVCMKNSS 1779
            :|||::|....|||:..:|.::.|::|...|:|||:.::|||||||||:.:|
Frog  1618 RGTCNYYANTYSFWLATIEKNEMFKKPTPSTLKAGDLRTHVSRCQVCMRRTS 1669

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	30/56 (54%)
		Collagen	322..380	CDD:189968	24/57 (42%)
		Collagen	413..465	CDD:189968	17/56 (30%)
		Collagen	499..561	CDD:189968	23/69 (33%)
		Collagen	574..632	CDD:189968	23/63 (37%)
		Collagen	657..714	CDD:189968	28/56 (50%)
		Collagen	765..824	CDD:189968	29/58 (50%)
		Collagen	854..911	CDD:189968	27/62 (44%)
		Collagen	884..943	CDD:189968	25/61 (41%)
		Collagen	923..982	CDD:189968	28/58 (48%)
		Collagen	1028..1085	CDD:189968	26/65 (40%)
		Collagen	1229..1287	CDD:189968	28/60 (47%)
		Collagen	1318..1376	CDD:189968	30/65 (46%)
		Collagen	1399..1458	CDD:189968	29/71 (41%)
		Collagen	1477..1534	CDD:189968	26/59 (44%)
		C4	1555..1662	CDD:128421	59/108 (55%)
		C4	1663..1777	CDD:128421	67/113 (59%)
col4a1	XP_002933064.3	Collagen	546..600	CDD:396114	27/56 (48%)
		Collagen	579..637	CDD:396114	27/60 (45%)
		Collagen	675..733	CDD:396114	30/61 (49%)
		PRK14959	<807..929	CDD:184923	58/128 (45%)
		Collagen	848..900	CDD:396114	23/54 (43%)
		Collagen	1066..1120	CDD:396114	22/54 (41%)
		Collagen	1117..1171	CDD:396114	25/53 (47%)
		Collagen	1238..1294	CDD:396114	28/55 (51%)
		Collagen	1355..1405	CDD:396114	22/49 (45%)
		Collagen	1383..1439	CDD:396114	29/67 (43%)
		C4	1445..1551	CDD:396133	56/105 (53%)
		C4	1555..1665	CDD:396133	64/110 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	163	1.000	Domainoid score	I3912
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1305	1.000	Inparanoid score	I154
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	1	1.000	-	-		otm48261
Panther	1	1.100	-	-	LDO	PTHR24023
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2460
SonicParanoid	1	1.000	-	-		X1239
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.100