DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Col4a1 and si:dkey-61l1.4

DIOPT Version :9

Sequence 1:	NP_723044.1	Gene:	Col4a1 / 33727	FlyBaseID:	FBgn0000299	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	XP_021332163.1	Gene:	si:dkey-61l1.4 / 100004431	ZFINID:	ZDB-GENE-070705-483	Length:	1813	Species:	Danio rerio

Alignment Length:	1488	Identity:	542/1488 - (36%)
Similarity:	676/1488 - (45%)	Gaps:	348/1488 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    51 IDDSYDIVDSAG----------------VARGDLPPKNCTAGYAGCVPK-----CIAEKGNRGLP 94
            :|.|.::..|||                ...|.|......:||:..|.|     ..::||...|.
Zfish   383 LDSSLNVKTSAGQKDLLRPTVHPELNQLTEEGFLLSSQSISGYSSQVVKQPSVDVSSQKGYEMLI 447

  Fly    95 GP------------------LGPTGLKGEM--GFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSP 139
            |.                  ||..|.:..:  |.||..||.|.:|..|:.|..|.:|||   ||.
Zfish   448 GTVRADNKFSRVIRKHGDVILGKDGRRYRLLSGPPGPVGPPGKRGCAGNRGYIGYKGDK---GSQ 509

  Fly   140 GLHGQAGVPGVQGPAGNPGAPGI----NGKDGCDGQDGIPGLEGL-SGMPGPRGYAGQLGSKGEK 199
            |:.|..|:.|:|||.|.||.|.:    |..:........|..:.| :|.|..   .||.|..||.
Zfish   510 GVQGMDGLRGIQGPPGPPGLPALYLWRNTDEDWAAFRRTPYYQLLAAGWPRE---TGQPGPMGET 571

  Fly   200 GEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCY 264
            |.|            |.||..|..|.:||   ||.:|:.|..||.|.:|..|..|..|.||..
Zfish   572 GRP------------GLPGLPGDPGQSGP---PGRRGDMGGMGPKGVRGRAGTQGRDGRKGLD-- 619

  Fly   265 GPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGE 329
                                                |.:|.||:.|.:|..|.:|||...|:|||
Zfish   620 ------------------------------------GIRGSPGIPGLQGPRGYKGDTAPPGEKGE 648

  Fly   330 KGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPG 394
            :|..|.||.||.:      |.|||||.:||.|..|.      .|.||..|..|..|:.|.|||
Zfish   649 EGFLGAPGPRGDK------GQTGQKGSKGEMGFTGF------SGPPGFVGLQGVEGIPGQPGP-- 699

  Fly   395 GGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGT 459
              :|.||..|.:||.|.||||      |..||.|.||.||.:|.|
Zfish   700 --KGDPGHDGKRGPAGTVGAP------GATGLVGAQGVNGSEGDA-------------------- 736

  Fly   460 AGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGA 524
                   |..||||..||.||              :|:.|..|.||.|||:
Zfish   737 -------GPAGPIGRKGPQGP--------------RGTDGIRGPPGQPGLQ-------------- 766

  Fly   525 PGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGI 589
                     |.||..|..|.|||.|:.|..|.:|..||:|.:         |..||.|..|.||:
Zfish   767 ---------GLPGQGGPQGLKGDIGKEGPVGNRGLQGIEGPM---------GMTGDPGPKGFPGV 813

  Fly   590 PGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKG 654
            .|..|..|..||.|..|::|..|..|..||.||:|:.|.|||||..|:
Zfish   814 TGNRGPDGEKGEEGPKGKKGPRGAPGIMGPQGERGQSGLTGLPGVKGQ----------------- 861

  Fly   655 DKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQS 719
                   |||.|:||..|..|..|:.|.:|:.|.:||.|.:|..|:.|:.||||:.|.|||||
Zfish   862 -------PGAAGIQGIDGEVGPQGMLGKEGQKGSRGEPGGNGRTGSKGSRGRAGQHGPPGIPG-- 917

  Fly   720 IKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGE 784
            |.|..|..|.:|.:|..|:.|.||..|.|             |:||..|..|:.|.||:.|..|.
Zfish   918 IVGLRGSVGAEGPEGKPGTQGVSGSVGNP-------------GDKGPHGLRGVIGEPGKQGPSGS 969

  Fly   785 RGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPG 849
            :|..|..|..|.||||   |.:|..|..|..|.||.||:.|.||..||||.||..|:   |||||
Zfish   970 QGPPGRPGQDGSQGPP---GEKGFTGKAGLAGAQGPVGMYGYPGAIGLRGPPGHTGE---RGEPG 1028

  Fly   850 ISRPGPMGPPGLN---GLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKG 911
            |.  ||:||||..   ||.|..|:||..||.|..|..|..|:.|..|.:||||..|..|..|..|
Zfish  1029 IR--GPLGPPGKRGPPGLAGPAGERGSRGPPGRQGETGPTGFQGLPGPSGLPGAEGVTGKQGPPG 1091

  Fly   912 DVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAP 976
            .:||.|..|..||.|:|            |.|||.|::|..|.||::|.||..|..|..|:.|..
Zfish  1092 SLGPQGSKGKQGPIGLP------------GSPGSSGMIGQTGEKGEQGVPGPAGDPGSVGLYGPQ 1144

  Fly   977 GKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGE 1041
            |..|..|:.|.:|:|||            ||..||.|.||..|.:|:|   |..|.||:.|.||.
Zfish  1145 GHPGRVGLEGPTGSKGD------------VGSPGPKGKPGPNGFQGEQ---GPQGSQGVLGAPGP 1194

  Fly  1042 KGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGE 1106
            :|.:|  |: ||||:.|:...|||||:||..|..|..||.|..|..||:||
Zfish  1195 RGKEG--GI-GPPGITGNQGPKGQKGDPGHRGFSGQQGPPGLLGDGGERGL-------------- 1242

  Fly  1107 KGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPG 1171
            ||::|..|..|::|:.|:.|..||.|:      :|.:|..|.|||.|..|..|..|:||..||||
Zfish  1243 KGNKGYLGSQGQNGLMGKIGPSGLMGL------EGLMGLRGPPGANGPPGPKGETGSPGPNGYPG 1301

  Fly  1172 DRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGY 1236
            |:|::|..|..||.|:.|:.||:|:      .||||.:|:.|..|.|
Zfish  1302 DQGNQGLAGRRGLQGVMGKAGPLGV------VGPKGPQGLMGPKGFP------------------ 1342

  Fly  1237 TGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGL 1301
             |::|.:||.|..||.|:.|..|..|..||.|..|..|:|   |::||.|.:|.||.  :|.||.
Zfish  1343 -GQEGPKGEPGRRGPPGLKGPPGLTGNNGPSGFPGKEGLP---GELGPDGPVGPPGP--QGPKGK 1401

  Fly  1302 PGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGP---IGPAGSKGERGLAGSPGQPGQDG 1363
            ||..||||.:|..|.....|..|..|.||..|..|..||   .||.|.||::|..|:.|.||:.|
Zfish  1402 PGPQGRNGPKGQKGNDARKGSSGKTGHAGRRGKAGKAGPRGLRGPKGHKGQQGAQGNTGPPGRQG 1466

  Fly  1364 FPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPV 1428
            ..|.||::|.      |||.|..|||   |:||||||.||   ||..|.||:.|..|:.|..||
Zfish  1467 QYGLPGIRGQ------KGENGAKGFE---GEKGDRGLLGP---PGGDGLKGNRGLQGVKGRPGP- 1518

  Fly  1429 GAPGERGFTGPKGRDGRDGTPGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPG 1486
              .||:|..||.|:.|..|.||:||..||.|:...||..|..|:.||.||.|.||.||
Zfish  1519 --KGEQGDIGPTGQRGTPGLPGMPGLFGEKGLKGYPGLTGPKGKRGRQGPPGLPGPPG 1574

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a1	NP_723044.1	Collagen	104..161	CDD:189968	25/58 (43%)
		Collagen	322..380	CDD:189968	23/57 (40%)
		Collagen	413..465	CDD:189968	13/51 (25%)
		Collagen	499..561	CDD:189968	19/61 (31%)
		Collagen	574..632	CDD:189968	25/57 (44%)
		Collagen	657..714	CDD:189968	23/56 (41%)
		Collagen	765..824	CDD:189968	25/58 (43%)
		Collagen	854..911	CDD:189968	27/59 (46%)
		Collagen	884..943	CDD:189968	21/58 (36%)
		Collagen	923..982	CDD:189968	22/58 (38%)
		Collagen	1028..1085	CDD:189968	27/56 (48%)
		Collagen	1229..1287	CDD:189968	20/57 (35%)
		Collagen	1318..1376	CDD:189968	24/60 (40%)
		Collagen	1399..1458	CDD:189968	27/58 (47%)
		Collagen	1477..1534	CDD:189968	7/10 (70%)
		C4	1555..1662	CDD:128421
		C4	1663..1777	CDD:128421
si:dkey-61l1.4	XP_021332163.1	LamG	<78..217	CDD:328935
		Collagen	563..629	CDD:189968	31/118 (26%)
		Collagen	614..672	CDD:189968	29/101 (29%)
		Collagen	644..703	CDD:189968	31/74 (42%)
		Med15	696..>1109	CDD:312941	204/554 (37%)
		Glutenin_hmw	970..>1468	CDD:281191	236/585 (40%)
		Collagen	1127..1184	CDD:189968	26/71 (37%)
		Collagen	1427..1484	CDD:189968	26/62 (42%)
		Collagen	1460..1531	CDD:189968	40/85 (47%)
		COLFI	1603..1812	CDD:321934
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910