DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment vkg and col5a2a

DIOPT Version :9

Sequence 1:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster
Sequence 2:	NP_001139254.1	Gene:	col5a2a / 564821	ZFINID:	ZDB-GENE-030616-13	Length:	1491	Species:	Danio rerio

Alignment Length:	1703	Identity:	573/1703 - (33%)
Similarity:	711/1703 - (41%)	Gaps:	480/1703 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 LGSLVSVTLADGKICNTTLCDCKGIKGRMGAPGPIGVPGLEGPAG----DIGPPGRAGPLGEKGD 79
            :.:...|.:..|:.|  .:|.....:|..|.||.....|.:|..|    .:|..||.||:|..|.
Zfish    74 VSNCAKVVIPPGECC--PVCQGDAAEGGNGRPGGRIAKGQKGEPGVVPHIVGIRGRPGPMGPPGA 136

  Fly    80 VGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGP------------------------RG 120
            .|..|:.|.||..|..||.|..|.|||.|..||||.|||                        .|
Zfish   137 PGFRGDSGPKGRPGLRGPPGYDGEPGIPGHPGEPGPPGPPPGGELASHMAGGFADKMGPQALMPG 201

  Fly   121 IDGCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGNRGETGQPGGVGPPG 185
            :.|..|..|..||:|:||.:|.:||||..|..||.|.||:           ||..|.||..|..|
Zfish   202 VRGEAGTRGPPGPNGSPGPSGPQGPPGDVGDPGPMGGAGQ-----------RGPEGPPGKPGEDG 255

  Fly   186 FDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDT 250
            ..|..||.|:.|:.|..|.:|.||.|                 ||||.||.
Zfish   256 EAGTPGSAGEMGFPGSPGGRGFPGTP-----------------GPPGLKGH-------------- 289

  Fly   251 LKGYKGYVGLQGDEGPQGPTGEQGAVGRNGLPGARGEIGGPGERGKPGKDGEPGRFGDKGMKGAP 315
             :|::|..||:|:.|..|..||.|..|..|.||..|..|.|||||:||..|..|..|.:|..|.|
Zfish   290 -RGHQGPDGLKGERGALGSKGESGGSGPMGAPGPMGPRGMPGERGRPGPGGAAGTRGGQGNMGKP 353

  Fly   316 GWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGDIGPPGEQGPPGL 380
            |..|..|::|:|      |:.|.||::|..||.|:..|...:...|..|.||..||.|:|||.|.
Zfish   354 GPMGPMGINGAP------GYPGSPGMKGQPGPTGVRGPEGPQGQRGETGHQGRSGPTGQQGPMGT 412

  Fly   381 PGKPGRRGPIGLAGQSGDPGLNGSRGPPGRSERGEAGDYGFIGPPGPQGPPGEAGLPGRYGLHGE 445
            .|.||.:||:|:.|..|..||.|..|||              ||.|..|.||..|..|..|:.|.
Zfish   413 DGGPGSKGPVGVPGPQGPVGLLGPTGPP--------------GPQGSTGQPGIKGQLGDLGVAGF 463

  Fly   446 PGQNVVGPKGEPGLNGQPGLEGYRGDRGEVGLPGDKGLPGEGYNIVGPP---GSQGPPGFRGLPG 507
            .|:  .||||||   |.||.:|..|.:||.|..|.:|.||.    :|||   |.:|.||.||.||
Zfish   464 KGE--AGPKGEP---GPPGSQGVLGPQGEEGKRGPRGDPGS----IGPPGPVGERGAPGNRGFPG 519

  Fly   508 DDGYNGLRGLPGEKGLRGDDCPVCNAGPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQGNP 572
            .||..|.:|..|::|         .:|..||:|..||.|..|..|..||.||||..||||.:|.
Zfish   520 QDGLQGPKGAQGDRG---------TSGTSGPKGSTGDPGRTGEPGLPGARGLTGTPGVQGAEGK- 574

  Fly   573 GRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEKGRDGSD 637
                    |||.|.|||.|:.|.||..|        .:|.||..|..|.:|.:||.|:.|..||.
Zfish   575 --------PGPLGAPGEDGRPGPAGSTG--------TRGPIGTMGPIGPKGFSGDPGKAGEQGSA 623

  Fly   638 GSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGERGDDGD 702
            |..|:||..|:.|:.|.|                              |.||..|..||||:.|.
Zfish   624 GVPGQRGPPGKDGEVGPA------------------------------GPPGPHGTAGERGEQGP 658

  Fly   703 TGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVIPGPVGA 767
            .|..|.:|.|.|      .|.|||.|..|.:|:.               |:.||.|::  ||.|.
Zfish   659 PGMHGFQGLPGP------AGAPGEGGKPGDQGIP---------------GEGGPVGQI--GPRGE 700

  Fly   768 KGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVG 832
            :|.||..|:.|..|.||..|..|.||.|      |.:|.|         ||.|..|.:|.||..|
Zfish   701 RGIPGERGELGPAGLPGAKGIPGAPGPD------GPKGSP---------GPTGTLGNQGPPGLQG 750

  Fly   833 APGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKG 897
            .||:.|::|.|||   ||::|..|..|..|.||:.|.||  |.||.||.|         |||..|
Zfish   751 MPGERGISGPPGP---KGDRGAIGEKGSEGTPGSDGARG--LTGPIGPAG---------PHGPSG 801

  Fly   898 QKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQ 962
            :|||.|..|..|..|::                  .:|||.|.|
Zfish   802 EKGEPGPKGPPGPQGSR------------------AMPGSRGEP--------------------- 827

  Fly   963 GDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKGETGNP---GFPGRPGAKGVAAYSGIKGD 1024
               ||.|..|..||.||.|..||.|.||.   ||:||:.|:|   |..|.||.:|....:|.||.
Zfish   828 ---GPIGAVGFVGPPGLDGQPGPKGPPGE---PGQKGDAGSPGPQGLAGPPGPQGKLGVAGPKGG 886

  Fly  1025 DGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGL 1089
            .|..|..||.|:||:.|..|..||.              |.:|.|||.||||:.|..|.|||.|.
Zfish   887 RGTQGAPGPTGFPGSAGRVGPPGPT--------------GPIGEPGPLGLPGKEGPPGLRGDHGP 937

  Fly  1090 PGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRG 1154
            ||:||..|.||.   .|.||..|.:|..|.   |||.||.||.|.:               |.||
Zfish   938 PGRQGERGPPGP---AGGPGDKGDSGEDGP---PGPDGPPGPAGTT---------------GQRG 981

  Fly  1155 LIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGE 1219
            ::|..|::||:   |..|:||.             .|..|.||..|..||.||.|.:|.|||.|.
Zfish   982 IVGLPGQRGER---GMAGLPGP-------------AGPPGKQGTSGAPGDKGPPGPVGVPGANGP 1030

  Fly  1220 RGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGP 1284
            |||.|..|..|.||..|.:|..|.||.||.|        |.|||            .|..|.|||
Zfish  1031 RGDPGPDGPAGSDGPPGKEGVIGAQGDRGDP--------GPEGV------------LGTVGQPGP 1075

  Fly  1285 PGENGPNGAIGHRG-PQIQGPPGPQGDVGFPGAPGHNGRHGLIGPKGELGDMGRQGERGESGYAI 1348
            ||..|..|.:|.|| |..:||.||         ||..|:.||.||:|..||
Zfish  1076 PGPVGATGGVGKRGEPGSKGPSGP---------PGPAGKRGLTGPQGPRGD-------------- 1117

  Fly  1349 VGRQGDIGDIGFQGEPGWDGAKGEQGYPGLPGKNGRVGAPGPRGPTGDAGWGGIDGMDGLVGPKG 1413
               :|||||.|.:|:.|..|..|.||.||.||..|..||                  .|:|||.|
Zfish  1118 ---KGDIGDHGERGQKGHRGFAGLQGLPGSPGTTGEQGA------------------TGIVGPSG 1161

  Fly  1414 QPGVTYSYSMARPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQG 1478
            |.|        .||..|.||.:|:.|:.|..|.|            |.||..|::   |.:||.|
Zfish  1162 QRG--------PPGPIGPPGKEGYIGQPGPMGPP------------GSRGSSGDI---GPEGPPG 1203

  Fly  1479 QRGDKGYMGLTGAPGLRGLPGPQGEP----------------APAPPAPKSRGFIFARHSQSVHV 1527
            :            ||..|.|||.|.|                .|:||          .::|...:
Zfish  1204 E------------PGPPGPPGPAGPPTAAVDDLFGGAHDFDSGPSPP----------DNNQDEAL 1246

  Fly  1528 PQCPANTNLLWEGYSLSGNVAASRAVGQDL----GQSGSCMMRFTTMPYMLCDITNVCHFAQNND 1588
            |.  :|:..:::  :..|..|..:|:...:    ...||     ...|...|:....| :.|...
Zfish  1247 PM--SNSTSVFQ--ADPGVQATLKALSSQIDNMRSPDGS-----RKHPARTCEDLKQC-YPQKKS 1301

  Fly  1589 DSLWLSTAEPMPMTMTPIQG--RDLMKYISRCVVCETTTRIIALHSQSMSIP-----DCPGGWEE 1646
            ...|:.          |.||  .|.:|     :.|...|....:.:...|||     ..||. :.
Zfish  1302 GEYWVD----------PNQGSAEDAIK-----IYCNMDTGETCISAIPSSIPRKSWWSSPGN-KP 1350

  Fly  1647 MW-----TGYSYF 1654
            :|     .|.:||
Zfish  1351 VWFGANMNGGTYF 1363

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	30/86 (35%)
		Collagen	102..152	CDD:189968	27/73 (37%)
		Collagen	277..334	CDD:189968	24/56 (43%)
		Collagen	534..593	CDD:189968	28/58 (48%)
		Collagen	814..871	CDD:189968	24/56 (43%)
		Collagen	957..1014	CDD:189968	24/59 (41%)
		Collagen	990..1049	CDD:189968	25/61 (41%)
		Collagen	1070..1128	CDD:189968	28/57 (49%)
		C4	1515..1624	CDD:128421	19/114 (17%)
		C4	1625..1737	CDD:128421	10/40 (25%)
col5a2a	NP_001139254.1	VWC	36..91	CDD:278520	3/18 (17%)
		Collagen	366..425	CDD:189968	26/58 (45%)
		Collagen	501..560	CDD:189968	27/67 (40%)
		Collagen	546..604	CDD:189968	31/74 (42%)
		Collagen	690..747	CDD:189968	27/73 (37%)
		Collagen	723..780	CDD:189968	28/74 (38%)
		COLFI	1260..1490	CDD:279718	26/126 (21%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	287	1.000	Domainoid score	I1547
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810