DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment vkg and Col5a3

DIOPT Version :9

Sequence 1:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster
Sequence 2:	NP_058615.1	Gene:	Col5a3 / 53867	MGIID:	1858212	Length:	1739	Species:	Mus musculus

Alignment Length:	1758	Identity:	603/1758 - (34%)
Similarity:	741/1758 - (42%)	Gaps:	474/1758 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    85 EQGEKGHRGDIGPKGEMG----------YPGI--MGKSGEPGTPGPRGIDGCDGRPGMQ--GPSG 135
            |:|| |....:|||....          :||.  .|..|||.|.          ..|.|  ||:|
Mouse   341 EEGE-GGGPTMGPKFRAAEQSLQTEFQIFPGAGEKGAKGEPATV----------EQGQQFEGPAG 394

  Fly   136 APGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGYAG 200
            |||..|:.||.|.|                                |||||.||||..|..|..|
Mouse   395 APGPRGISGPSGPP--------------------------------GPPGFPGDRGLPGPAGLPG 427

  Fly   201 LTGEKGDPGLPGPKGDTGAVSELPY-----SLIGPP-----------------GAKGEPGD-SLS 242
            :      ||:.|.:|..|.|..:|:     |:.|||                 ..||.||. .|:
Mouse   428 I------PGIDGARGLPGTVIMMPFHFASSSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLT 486

  Fly   243 GVLKPDDT-LKGYKGYVGLQGDEGPQGPTGEQGAVGRNGLPGARGEIGGPGERGKPGKDGEPGRF 306
            |  :|... |.||.|..|..|:.|||||.|.||..|..|..|..|..|..|.||.|
Mouse   487 G--RPGPVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLP--------- 540

  Fly   307 GDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGDIGP 371
            ||.|.||..|:.|..||.|..|:||:.|..|.||..|..|..|:..|      |||.|..|:.||
Mouse   541 GDTGPKGDRGFDGLPGLPGEKGQRGDFGRVGQPGPPGEDGVKGLQGP------PGPTGQAGEPGP 599

  Fly   372 PGEQGPPGLPGKPGRRGPIGLAGQSGDPGLNGSRGPPGRSERGEAGDYGFIGPPGPQGPPGEAGL 436
            .|..||.||||..||.   |:.|..|.||..|:.||||..           ||||.||..|..|:
Mouse   600 RGLIGPRGLPGPLGRP---GVTGSDGAPGAKGNVGPPGEP-----------GPPGQQGNHGSQGI 650

  Fly   437 PGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGYRGDRGEVGLPGDKGLPGEGYNIVGPPGSQGPPG 501
            ||..|..|.||:.  ||.|.||:.|.||.||..|..|..|..|:||..       |||||.||.|
Mouse   651 PGPQGPIGTPGEK--GPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQ-------GPPGSAGPRG 706

  Fly   502 FRGLPGDDGYNGLRGLPGEKGLRGDDCPVCNAGPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQ 566
            :.||.|..|.:|.|||.||||.||:|......|..||:|..|:.|.||..|..|..|..||.|:.
Mouse   707 YPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLP 771

  Fly   567 GLQGNPGRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEK 631
            |.:|.||.||.||..|..|:||.||:.|..|..|        ..|.:|..|:.|.||..|..||:
Mouse   772 GDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIG--------FPGPLGPLGEKGKRGKAGQPGEE 828

  Fly   632 GRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGE 696
            |..|:.|::|:||:.|..|..|..|..|::|.||..|..|.||         |.|.||:.|.||.
Mouse   829 GERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPG---------LQGPPGFPGPKGP 884

  Fly   697 RGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVI 761
            .|..|..|..|     :|||    .||.|..|.|||.|..|..|.||.:|..|.:|:|||
Mouse   885 PGPQGKDGISG-----HPGQ----RGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGP----- 935

  Fly   762 PGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKG 826
                     |||.|..|:||.||:.||:|..|..|.:|..|:.|.||.               :|
Mouse   936 ---------PGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGP---------------RG 976

  Fly   827 FPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQA 891
            |||..||||.      |||.|:||::||.|.||..|.||.:|.     |||.|..|.||::|.|.
Mouse   977 FPGPQGAPGD------PGPIGLKGDKGPPGPVGANGSPGERGP-----VGPSGGIGLPGQSGGQG 1030

  Fly   892 PHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGI---PGLPGMIGEIGER 953
            |.|..|:||.                  .|.||..|..|..|:||.||:   .|..|..||.|::
Mouse  1031 PIGPAGEKGS------------------PGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGDK 1077

  Fly   954 GEIGYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKGETGNPGFPGRPGAKGVAAY 1018
            ||:|..|.:|..|.:|..|..||.|:.|..|..|.|||:|..||:|.   ||..|:
Mouse  1078 GEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHSGLPGADGAQGRRGP---PGLFGQ--------- 1130

  Fly  1019 SGIKGDDGESGLTGPIGYP---GAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGL 1080
               |||||..|..|.||.|   |.||..|::|.|||              |||.||:|.||..|.
Mouse  1131 ---KGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGD--------------VGSMGPHGAPGPRGP 1178

  Fly  1081 KGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQ---GPKGPQGESGVVGLDG 1142
            .|..|..|.||..|..|:|||.|..|.||..|..|..|..|.|||:   |.||..|.||..|..|
Mouse  1179 PGPSGSEGPPGLPGGVGQPGAVGEKGEPGDAGDAGPPGIPGIPGPKGEIGEKGDSGPSGAAGPPG 1243

  Fly  1143 RNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDM-- 1205
            :.|..|:.|.:|.:|..|..|:.|..|:.|:|                |..|:.||:|..||:
Mouse  1244 KKGPPGEDGSKGNMGPTGLPGDLGPPGDPGVP----------------GIDGIPGEKGNAGDIGG 1292

  Fly  1206 -GPIGFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGR 1269
             ||.|..|.|||:|..|..|..|::|.:|.||.||.:||.||.||||.|
Mouse  1293 PGPPGASGEPGARGLPGKRGSPGRMGPEGREGEKGAKGDAGPDGPPGRT---------------- 1341

  Fly  1270 AGRPGHFGQKGAPGPPGENGPNGAIGHRGPQIQGPPGPQGDVGFPGAPGHNGRHGLIGPKGELGD 1334
                   |..||.||||..||:|        :.|.|||.|:.|..|.|      |||||.|.||.
Mouse  1342 -------GPIGARGPPGRIGPDG--------LPGIPGPVGEPGLLGPP------GLIGPPGPLGP 1385

  Fly  1335 MGRQGERGESGYAIVGRQGDIGDIGFQGEPGWDGAKGEQGYPGLPGKNGRVGAPGPRGPTGDAGW 1399
            .|..|.:|::|..  |.:|.||.||..|.||..|.||:||.||:            :||
Mouse  1386 PGLPGLKGDAGPK--GEKGHIGLIGLIGPPGEAGEKGDQGLPGV------------QGP------ 1430

  Fly  1400 GGIDGMDGLVGPKGQPGVTYSYSMARPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGD 1464
                                      ||.:|:|||.|..|..|..|.||::|..||:|:
Mouse  1431 --------------------------PGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGS------ 1463

  Fly  1465 QGEVGYTGADGPQGQRGDKGYMGLTGAPGLRGLPGPQGEPAPAPPAPKSRGFIFARHSQSVHVPQ 1529
                  .|:.|..|.|||         ||..|.|||.|.||      :..|.   |..:||
Mouse  1464 ------KGSPGSLGPRGD---------PGPAGPPGPPGSPA------EVHGL---RRRRSV---- 1500

  Fly  1530 CPANTNLLWEGY-----SLSGNVAASRAVGQDLGQSGSCMMRFTTMPYMLCDITNVCHFAQNNDD 1589
                |:.|..|.     ||:......:.:.:.||.:.|        |.::|...:..| ....|.
Mouse  1501 ----TDTLEGGLEEVMASLNSLSLELQQLQRPLGTAES--------PGLMCRELHRDH-PHLPDG 1552

  Fly  1590 SLWLSTAEPMPMTMTPIQG--RDLMKYI-------SRCVVCETTTRIIALHSQSMSIPDCPGGWE 1645
            ..|:.          |.||  ||..|..       ..|:..:.....:.|.|.|.   :.||||
Mouse  1553 EYWID----------PNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSR---EKPGGW- 1603

  Fly  1646 EMWTGYSYF-----MSTLDNVGGVGQNLVSPGSCLEEFRAQPVIECHGHGRCNYYDALASFWLTV 1705
                 ||.|     .|.:|..|       ||.:.: :.....::....|.|..|....:..||..
Mouse  1604 -----YSTFRRGKKFSYVDADG-------SPVNVV-QLTFLKLLSAAAHQRFTYICQNSVAWLDE 1655

  Fly  1706 IE--------------EQDQFVQPRQQTLKADFTSKISRCTVCR-RRGNSFVARTAKSRADASS- 1754
            ..              |:..|.|....|:|.....       || |:|.      ||:..:.||
Mouse  1656 AAGDHRHSIRFQGTNWEELSFNQTTAATIKVSHDG-------CRVRKGQ------AKTLFEFSSS 1707

  Fly  1755 -GF 1756
             ||
Mouse  1708 VGF 1710

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	14/45 (31%)
		Collagen	102..152	CDD:189968	20/63 (32%)
		Collagen	277..334	CDD:189968	22/56 (39%)
		Collagen	534..593	CDD:189968	28/58 (48%)
		Collagen	814..871	CDD:189968	23/56 (41%)
		Collagen	957..1014	CDD:189968	22/56 (39%)
		Collagen	990..1049	CDD:189968	24/61 (39%)
		Collagen	1070..1128	CDD:189968	27/60 (45%)
		C4	1515..1624	CDD:128421	24/122 (20%)
		C4	1625..1737	CDD:128421	27/131 (21%)
Col5a3	NP_058615.1	LamG	32..211	CDD:304605
		Collagen	532..590	CDD:189968	27/72 (38%)
		Collagen	568..626	CDD:189968	28/66 (42%)
		Collagen	679..731	CDD:189968	28/58 (48%)
		Collagen	769..827	CDD:189968	25/65 (38%)
		Collagen	805..864	CDD:189968	24/66 (36%)
		Collagen	1003..1060	CDD:189968	27/79 (34%)
		Collagen	1057..1115	CDD:189968	23/57 (40%)
		Collagen	1087..1147	CDD:189968	29/74 (39%)
		Collagen	1312..1364	CDD:189968	29/82 (35%)
		Collagen	1393..1443	CDD:189968	27/95 (28%)
		COLFI	1510..1737	CDD:279718	52/250 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	287	1.000	Domainoid score	I1573
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910