DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment vkg and COL4A3

DIOPT Version :9

Sequence 1:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster
Sequence 2:	NP_000082.2	Gene:	COL4A3 / 1285	HGNCID:	2204	Length:	1670	Species:	Homo sapiens

Alignment Length:	1858	Identity:	738/1858 - (39%)
Similarity:	886/1858 - (47%)	Gaps:	339/1858 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    18 LLGSLVSVTLA------DGKIC---NTTLCD-CKGIKGRMGAPGPIGVPGLEGPAGDIGPPGRAG 72
            ||..|:.|.||      .|.:|   ....|| .||.||..|.|||   ||..|..|..||.|..|
Human    12 LLLPLLLVLLAAAPAASKGCVCKDKGQCFCDGAKGEKGEKGFPGP---PGSPGQKGFTGPEGLPG 73

  Fly    73 PLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQGPSGAP 137
            |.|.||..|..|..|.||.||..|..|..|.||:.|..|..|..|..|:.||.|..|.||..|.|
Human    74 PQGPKGFPGLPGLTGSKGVRGISGLPGFSGSPGLPGTPGNTGPYGLVGVPGCSGSKGEQGFPGLP 138

  Fly   138 GQNGVRGPPGK---PGQQGPPGEAGEGGINSKGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGYA 199
            |..|..|.||.   .||:|.|.:..:..:::||..|..|..|..|..|||||.|..|..|..|:.
Human   139 GTLGYPGIPGAAGLKGQKGAPAKEEDIELDAKGDPGLPGAPGPQGLPGPPGFPGPVGPPGPPGFF 203

  Fly   200 GLTGEKGDPGLPGPKGDTGAVSELPY---------SLIGPPGAKGEPGDSLSGVLKPDD--TLKG 253
            |.      ||..||:|..|.:.|...         .|.||||..|....:|:|   ||:  .|||
Human   204 GF------PGAMGPRGPKGHMGERVIGHKGERGVKGLTGPPGPPGTVIVTLTG---PDNRTDLKG 259

  Fly   254 YKGYVGLQGDEGPQGPTGEQGAVGRNGLPG-----ARGEIGGPGERGKPGKDGEPGRFGDKGMKG 313
            .||..|..|:.||.||         :||||     .:|..|.||.:|||||||.||..|.:|:||
Human   260 EKGDKGAMGEPGPPGP---------SGLPGESYGSEKGAPGDPGLQGKPGKDGVPGFPGSEGVKG 315

  Fly   314 APGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTK---SLPGPIGSQGDIGPPGEQ 375
            ..|:.|..|.||..|::|:   .|.||.:   ||...||....|   ..|||.|.:|..||.|..
Human   316 NRGFPGLMGEDGIKGQKGD---IGPPGFR---GPTEYYDTYQEKGDEGTPGPPGPRGARGPQGPS 374

  Fly   376 GPPGLPGKPGRRGPIGLAGQSGDPGLNGS---RGPPGRSERGEAGDYGFI---------GPPGP- 427
            ||||:||.||...| ||.|..|.|||.||   ||.||:...|..|..|..         |||||
Human   375 GPPGVPGSPGSSRP-GLRGAPGWPGLKGSKGERGRPGKDAMGTPGSPGCAGSPGLPGSPGPPGPP 438

  Fly   428 ------QGPPGEAGLPGRYGLHGEPGQNVVGPKGEPGL-----------------------NGQP 463
                  :||||:.||||..|..|.||  |.||||||||                       .|.|
Human   439 GDIVFRKGPPGDHGLPGYLGSPGIPG--VDGPKGEPGLLCTQCPYIPGPPGLPGLPGLHGVKGIP 501

  Fly   464 GLEGYRGDRGEVGLPGDKGLPGEGYNIVGPPGSQ----------------------GPPGFRGLP 506
            |.:|..|.:|..|.||:.||||    ..|.||:|                      |.||.||.|
Human   502 GRQGAAGLKGSPGSPGNTGLPG----FPGFPGAQGDPGLKGEKGETLQPEGQVGVPGDPGLRGQP 562

  Fly   507 GDDGYNGLRGLPGEKGLRGDDCPVCNAGPRGPRGQEGDTGYPGS-----------HGNRGAIGLT 560
            |..|.:|:.|.||.|||.|....:..:|.:|.:|..||.|.|||           :|.:|..||.
Human   563 GRKGLDGIPGTPGVKGLPGPKGELALSGEKGDQGPPGDPGSPGSPGPAGPAGPPGYGPQGEPGLQ 627

  Fly   561 GPRGVQGLQGNPGRAGHKG-------LPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGD 618
            |.:||.|..|.||.||.:|       :|||.|.||.||..|..||.|        ..|.:|..||
Human   628 GTQGVPGAPGPPGEAGPRGELSVSTPVPGPPGPPGPPGHPGPQGPPG--------IPGSLGKCGD 684

  Fly   619 SGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVY 683
            .|..|..|:.|..| .|..|..|.:|:.|..|..|..|..|:.||||..|:.|.||... .|.|.
Human   685 PGLPGPDGEPGIPG-IGFPGPPGPKGDQGFPGTKGSLGCPGKMGEPGLPGKPGLPGAKG-EPAVA 747

  Fly   684 LIGEPGYDGIKGERGDDGDTGFKGVKGEPN-PGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGL 747
            :.|.||..|..||||:.|:.|..|:.|.|. |       |.||.:|..||:|..|..|..
Human   748 MPGGPGTPGFPGERGNSGEHGEIGLPGLPGLP-------GTPGNEGLDGPRGDPGQPGPP----- 800

  Fly   748 RGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDG--GIGYKGQRGVPGQE 810
                |::||.|..|.||.||:|.||..|..||||..|..|..|:||:.|  ..|:.|:.|.||..
Human   801 ----GEQGPPGRCIEGPRGAQGLPGLNGLKGQQGRRGKTGPKGDPGIPGLDRSGFPGETGSPGIP 861

  Fly   811 VIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLV 875
            ..|||:||.|:.|..|.||.:|.||:                  ||.:|..|.||        .:
Human   862 GHQGEMGPLGQRGYPGNPGILGPPGE------------------DGVIGMMGFPG--------AI 900

  Fly   876 GPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGS---------IGFSGRRGLLGNAGL 931
            |||||.|.||..|::...|..|.||:.|:.|..|:.|.||:         ||..|..||.|.||.
Human   901 GPPGPPGNPGTPGQRGSPGIPGVKGQRGTPGAKGEQGDKGNPGPSEISHVIGDKGEPGLKGFAGN 965

  Fly   932 QGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGA---NG 993
            .|..|:.|:||:||:         .|..|..|..||.||.|:.|..|..|:.|..|.||:   .|
Human   966 PGEKGNRGVPGMPGL---------KGLKGLPGPAGPPGPRGDLGSTGNPGEPGLRGIPGSMGNMG 1021

  Fly   994 LPGRKGETGNPGFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDG 1058
            :||.||:.|..|||||.|..|:....|::||.||.|.:... .||.|      ||.||  |.|.|
Human  1022 MPGSKGKRGTLGFPGRAGRPGLPGIHGLQGDKGEPGYSEGT-RPGPP------GPTGD--PGLPG 1077

  Fly  1059 VAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFP 1123
            ..|:|||:|.|||   ||.         .|..|.:|.||.||:.||.|.||.:|..|.||..|..
Human  1078 DMGKKGEMGQPGP---PGH---------LGPAGPEGAPGSPGSPGLPGKPGPHGDLGFKGIKGLL 1130

  Fly  1124 GPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRG 1188
            ||.|.:||.|..|..|..|..|..||||..|:.|..||:||.|                  ..|.
Human  1131 GPPGIRGPPGLPGFPGSPGPMGIRGDQGRDGIPGPAGEKGETG------------------LLRA 1177

  Fly  1189 FTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGIT 1253
            ..|.:|..|.:|.:||.|..||.|.||.||..||.         |..|..|.:|..||.|.||..
Human  1178 PPGPRGNPGAQGAKGDRGAPGFPGLPGRKGAMGDA---------GPRGPTGIEGFPGPPGLPGAI 1233

  Fly  1254 LPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGEN-----GPNGAIGHRGPQIQGPPGPQGDVGF 1313
            :|.:.|:.|..|..|..|.|      |.|||||.:     |..|::||.||  :||||..||:|.
Human  1234 IPGQTGNRGPPGSRGSPGAP------GPPGPPGSHVIGIKGDKGSMGHPGP--KGPPGTAGDMGP 1290

  Fly  1314 PGAPGHNGRHGLIGPKGELGDMGRQGERGESGYAIVGRQGDIGDIGFQGEPGWDGAKGEQGYPGL 1378
            ||..|..|..||.||:                          ||.||||.|   |.|||:|.||.
Human  1291 PGRLGAPGTPGLPGPR--------------------------GDPGFQGFP---GVKGEKGNPGF 1326

  Fly  1379 PGKNGRVGAPGPRGPTGDAGWGGIDGMDGLVGPKGQPGVTYSYSMARPGDRGEPGLDGFQGEEGD 1443
            .|.   :|.|||.||.|..|..|..|...::...|.||        .||..||||:   |||.|.
Human  1327 LGS---IGPPGPIGPKGPPGVRGDPGTLKIISLPGSPG--------PPGTPGEPGM---QGEPGP 1377

  Fly  1444 GGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQGQRGDKGYMGLTGAPGLRGLPGPQGEPAPAP 1508
            .|.||.:|..|.||..|..|..|..|..|..|.:|.:|:.|..|..|.|||:|..|..|.||
Human  1378 PGPPGNLGPCGPRGKPGKDGKPGTPGPAGEKGNKGSKGEPGPAGSDGLPGLKGKRGDSGSPA--- 1439

  Fly  1509 PAPKSRGFIFARHSQSVHVPQCPANTNLLWEGYS---LSGNVAASRAVGQDLGQSGSCMMRFTTM 1570
             ...:|||:|.||||:..:|.||..|..|:.|:|   :.||   .||.|||||..|||:.|||||
Human  1440 -TWTTRGFVFTRHSQTTAIPSCPEGTVPLYSGFSFLFVQGN---QRAHGQDLGTLGSCLQRFTTM 1500

  Fly  1571 PYMLCDITNVCHFAQNNDDSLWLSTAEPMPMTMTPIQGRDLMKYISRCVVCETTTRIIALHSQSM 1635
            |::.|::.:||:||..||.|.||||...|||.|.||.||.|..|||||.|||.....||:|||:.
Human  1501 PFLFCNVNDVCNFASRNDYSYWLSTPALMPMNMAPITGRALEPYISRCTVCEGPAIAIAVHSQTT 1565

  Fly  1636 SIPDCPGGWEEMWTGYSYFMSTLDNVGGVGQNLVSPGSCLEEFRAQPVIECHGHGRCNYYDALAS 1700
            .||.||.||..:|.|:|:.|.|.....|.||.|.|||||||||||.|.:||||.|.||||....|
Human  1566 DIPPCPHGWISLWKGFSFIMFTSAGSEGTGQALASPGSCLEEFRASPFLECHGRGTCNYYSNSYS 1630

  Fly  1701 FWLTVIEEQDQFVQPRQQTLKADFTSK-ISRCTVCRRR 1737
            |||..:..:..|.:|...|:||....| ||||.||.::
Human  1631 FWLASLNPERMFRKPIPSTVKAGELEKIISRCQVCMKK 1668

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	26/58 (45%)
		Collagen	102..152	CDD:189968	22/52 (42%)
		Collagen	277..334	CDD:189968	28/61 (46%)
		Collagen	534..593	CDD:189968	31/76 (41%)
		Collagen	814..871	CDD:189968	18/56 (32%)
		Collagen	957..1014	CDD:189968	27/59 (46%)
		Collagen	990..1049	CDD:189968	24/61 (39%)
		Collagen	1070..1128	CDD:189968	23/57 (40%)
		C4	1515..1624	CDD:128421	61/111 (55%)
		C4	1625..1737	CDD:128421	57/112 (51%)
COL4A3	NP_000082.2	7S domain	29..42		2/12 (17%)
		Triple-helical region	43..1438		607/1588 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	49..78		15/31 (48%)
		Collagen	67..124	CDD:189968	25/56 (45%)
		Collagen	97..155	CDD:189968	23/57 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	167..469		132/328 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	502..1442		414/1107 (37%)
		Cell attachment site. /evidence=ECO:0000255	791..793		0/1 (0%)
		Collagen	830..885	CDD:189968	23/54 (43%)
		Cell attachment site. /evidence=ECO:0000255	996..998		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1154..1156		0/1 (0%)
		Collagen	1164..1219	CDD:189968	25/81 (31%)
		Cell attachment site. /evidence=ECO:0000255	1306..1308		0/27 (0%)
		Cell attachment site. /evidence=ECO:0000255	1345..1347		0/1 (0%)
		Collagen	1390..1438	CDD:189968	19/47 (40%)
		Epitope recognized by Goodpasture antibodies	1427..1444		7/20 (35%)
		Cell attachment site. /evidence=ECO:0000255	1432..1434		0/1 (0%)
		C4	1446..1552	CDD:279721	58/108 (54%)
		Required for the anti-angiogenic activity of tumstatin	1479..1557		46/80 (58%)
		C4	1556..1666	CDD:279721	56/109 (51%)
		Required for the anti-tumor cell activity of tumstatin	1610..1628		11/17 (65%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	133	1.000	Domainoid score	I5070
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.920