DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment vkg and Col5a1

DIOPT Version :9

Sequence 1:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster
Sequence 2:	NP_056549.2	Gene:	Col5a1 / 12831	MGIID:	88457	Length:	1838	Species:	Mus musculus

Alignment Length:	1677	Identity:	618/1677 - (36%)
Similarity:	725/1677 - (43%)	Gaps:	434/1677 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    49 APGPIGVPGLEGPAG-DIGPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGE 112
            :|..|| ||:  ||. |....|..||.||||..||                     |.|:    |
Mouse   426 SPSEIG-PGM--PANQDTIFEGIGGPRGEKGQKGE---------------------PAII----E 462

  Fly   113 P-----GTPGPRGIDGCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGNR 172
            |     |.|||.|..|..|.||..||:|..|..|.|||||:||..|..|..
Mouse   463 PGMLIEGPPGPEGPAGLPGPPGTTGPTGQMGDPGERGPPGRPGLPGADGLP-------------- 513

  Fly   173 GETGQPGGVGPPGF----------DGDRGSKG-----DTGYAGLTGEKGDPGLPGPKGDTGAVSE 222
                     ||||.          .||.||||     ....|....::....|.||.|
Mouse   514 ---------GPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAG------- 562

  Fly   223 LPYSLIGPPGAKGEPGDSLSGVLKPDDTLKGYKGYVGLQGDEGPQGPTGEQGAVGRNGLPGARGE 287
             |..|.|.||..|.||   ||.||            |..||.|||||.|.||..|..|.||.||.
Mouse   563 -PMGLTGRPGPMGPPG---SGGLK------------GEPGDMGPQGPRGVQGPPGPTGKPGRRGR 611

  Fly   288 IGGPGERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYD 352
            .|..|.||.||:.|..   ||:|.         |||.|.|||:|.      .|..|.:|||||
Mouse   612 AGSDGARGMPGQTGPK---GDRGF---------DGLAGLPGEKGH------RGDPGPSGPPGI-- 656

  Fly   353 PSLTKSLPGPIGSQGDIGPPGEQGPPGLPGKPGRR---GPIGLAGQSGDPGLNGSRGPPGRSERG 414
                   ||..|.:||   .||.||.||||:||.|   ||.|..|..|.||:.|..|.||  .:|
Mouse   657 -------PGDDGERGD---DGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPG--PKG 709

  Fly   415 EAGDYGFIGPPGPQGPPGEAGLPGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGYRGDRGEVGLPG 479
            ..|..|..||||.||.||..||||..|..|.||:.  ||.|:|||.|.||.:|..|..|:.|.||
Mouse   710 NVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEK--GPLGKPGLPGMPGADGPPGHPGKEGPPG 772

  Fly   480 DKGLPGEGYNIVGPPGSQGPPGFRGLPGDDGYNGLRGLPGEKGLRGDDCPVCNAGPRGPRGQEGD 544
            :||  |:     ||||.|||.|:.|..|..|.:|:|||.|.||.:|:|         |..|.:||
Mouse   773 EKG--GQ-----GPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGED---------GFPGFKGD 821

  Fly   545 TGYPGSHGNRGAIGLTGPRGVQGLQGNPGRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLR 609
            .|.   .|:||.||..||||..|.:|..||.|..|.|||.|..||.||:|..|..|..   |  |
Mouse   822 MGI---KGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYP---G--R 878

  Fly   610 KGEIGDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPG 674
            :|..|..|..|..|..|:.|.:|..|..|.:|:||.||.||:.|..|..|:.|..|..|.||.
Mouse   879 QGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGP-- 941

  Fly   675 RNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAK 739
                      .|.||.   :|..|..|.|||.|.||.|.|         ||:|      |:.|..
Mouse   942 ----------AGPPGE---RGPNGPQGPTGFPGPKGPPGP---------PGKD------GLPGHP 978

  Fly   740 GEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQR 804
            |::|..|.:|:.|..||     ||.||.:|..|.||..|::|.||.||..||.||.|..|.:|.:
Mouse   979 GQRGETGFQGKTGPPGP-----PGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTK 1038

  Fly   805 GVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQ 869
            |.||...:.|:.|||   |::|||||.|.||..|..      |:||.:||.
Mouse  1039 GDPGPAGLPGKDGPP---GLRGFPGDRGLPGPVGAL------GLKGSEGPP-------------- 1080

  Fly   870 RGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGL 934
                  |||||.|.|                     |:.|..||.|.||..||      .|.||.
Mouse  1081 ------GPPGPAGSP---------------------GERGPAGAAGPIGIPGR------PGPQGP 1112

  Fly   935 PGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKG 999
            ||..|..|||   ||.|.:|..|.:|.||.:|..||.|..||.|..||.|..|.||..|..|.||
Mouse  1113 PGPAGEKGLP---GEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKG 1174

  Fly  1000 ETGNPGFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDGVAGRKG 1064
            |.|.|                     |.:|..||||.||..||.|:
Mouse  1175 EQGPP---------------------GPTGPQGPIGQPGPSGADGE------------------- 1199

  Fly  1065 EVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQGPK 1129
                |||.   |:.||.||:||.   |.:|.||.||..||.|.||..|..|..|..|..||.||.
Mouse  1200 ----PGPR---GQQGLFGQKGDE---GSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPP 1254

  Fly  1130 GPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQG 1194
            ||:|.||..|.||..|..|..|..|.:||:||.||.||.   |:||.             .|..|
Mouse  1255 GPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGDP---GLPGE-------------GGPLG 1303

  Fly  1195 LQGERGEQGDMGPIGFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKG 1259
            .:|||||:|:.||.|..||||.||..||.|.      .|:.|..||.||.||.|.||   ||  |
Mouse  1304 PKGERGEKGEAGPSGAAGPPGPKGPPGDDGP------KGSPGPVGFPGDPGPPGEPG---PA--G 1357

  Fly  1260 DEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGPQIQGPPGPQGDVGFPGAPGHNGRHG 1324
            .:|..|..|..|.|   ||.|:|||.||.||:|..|.|     |||||.|..|..|..|..|..|
Mouse  1358 QDGPPGDKGDDGEP---GQTGSPGPTGEPGPSGPPGKR-----GPPGPAGPEGRQGEKGAKGEAG 1414

  Fly  1325 LIGPKGELGDMGRQGERGESG-YAIVGRQGDIGDIGFQGEPGWDGAKGEQGYPGLPGKNGRVGAP 1388
            |.||.|:.|.:|.||..|:.| ..:.|..|.:|:.|..|.||.||..|..|.||||      |..
Mouse  1415 LEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLP------GLK 1473

  Fly  1389 GPRGPTGDAGWGGIDGMDGLVGPKGQPGVTYSYSMARPGDRGEPGLDGFQGEEGDGGAPGLIGFQ 1453
            |..||.|:.|.   .|:.||:||.|:.|        ..||||.||..|..|.:||.|..|..|..
Mouse  1474 GDSGPKGEKGH---PGLIGLIGPPGEQG--------EKGDRGLPGPQGSSGPKGDQGITGPSGPL 1527

  Fly  1454 GQRGAVGYRGDQGEVGYTGADGPQGQRGDKGYMGLTGAPGLRGLPGPQGE---PAPAPPAPKSRG 1515
            |..|..|..|..|..|..|:.||.|.:|:.|:      |||.|.|||.||   |.|. .|.::|.
Mouse  1528 GPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGH------PGLPGPPGPPGEVIQPLPI-QASRTRR 1585

  Fly  1516 FIFARHSQSVHVPQCPANTNLLWEGYSLSGNVAASRAVGQD--LGQSGSCMMRFTTM-------- 1570
            .|.|              :.||.:|   :|......|.|.:  .|...|..:....|
Mouse  1586 NIDA--------------SQLLDDG---AGESYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQ 1633

  Fly  1571 -PYMLCDITNVCHFAQNNDDSLWLSTAEPMPMTMTPIQG--RDLMKYI-------SRCVVCETTT 1625
             |...|....:|| ....|...|:.          |.||  ||..|..       |.||..:..:
Mouse  1634 NPARTCKDLQLCH-PDFPDGEYWVD----------PNQGCSRDSFKVYCNFTAGGSTCVFPDKKS 1687

  Fly  1626 ---RIIALHSQSMSIPDCPGGWEEMWTGYSYF-----MSTLDNVG---GVGQ 1666
               ||.:...::      ||.|      :|.|     :|.:|..|   ||.|
Mouse  1688 EGARITSWPKEN------PGSW------FSEFKRGKLLSYVDAEGNPVGVVQ 1727

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	19/64 (30%)
		Collagen	102..152	CDD:189968	25/54 (46%)
		Collagen	277..334	CDD:189968	24/56 (43%)
		Collagen	534..593	CDD:189968	27/58 (47%)
		Collagen	814..871	CDD:189968	19/56 (34%)
		Collagen	957..1014	CDD:189968	24/56 (43%)
		Collagen	990..1049	CDD:189968	19/58 (33%)
		Collagen	1070..1128	CDD:189968	26/57 (46%)
		C4	1515..1624	CDD:128421	26/128 (20%)
		C4	1625..1737	CDD:128421	13/53 (25%)
Col5a1	NP_056549.2	TSPN	39..230	CDD:214560
		Nonhelical region. /evidence=ECO:0000250	231..443		8/19 (42%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	242..545		54/169 (32%)
		Interrupted collagenous region. /evidence=ECO:0000250	444..558		47/161 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	559..1574		519/1297 (40%)
		Triple-helical region. /evidence=ECO:0000250	559..1570		517/1293 (40%)
		Collagen	726..>767	CDD:189968	21/42 (50%)
		Collagen	796..855	CDD:189968	30/70 (43%)
		Collagen	829..886	CDD:189968	28/61 (46%)
		Collagen	871..930	CDD:189968	24/63 (38%)
		Nonhelical region. /evidence=ECO:0000250	1571..1605		11/51 (22%)
		COLFI	1610..1836	CDD:279718	31/141 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	287	1.000	Domainoid score	I1573
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.900