DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment vkg and Col4a4

DIOPT Version :9

Sequence 1:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster
Sequence 2:	NP_031761.1	Gene:	Col4a4 / 12829	MGIID:	104687	Length:	1682	Species:	Mus musculus

Alignment Length:	1824	Identity:	739/1824 - (40%)
Similarity:	885/1824 - (48%)	Gaps:	307/1824 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    34 NTTLCDCKGIKGRMGAPGPIGVPGLEGPAGDIGPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPK 98
            |.::|.|...||..|.|||:|..|..||.|.:||.|..|..||:||.|..|..|||   ||.||.
Mouse    43 NCSVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEK---GDKGPT 104

  Fly    99 GEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGI 163
            |..|:||:.|..|.||.|||||..|.||..|.:|..|.||:.|..||.|.|||.|..||.|....
Mouse   105 GVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVY 169

  Fly   164 NSKGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLI 228
            .:.|.||.:|:.|.|   ||||..|.||::|.   .|..|..|.|||.||.|..|:
Mouse   170 ITGGVKGIQGDRGDP---GPPGLPGSRGAQGS---PGPMGHAGAPGLAGPIGHPGS--------- 219

  Fly   229 GPPGAKGEPGDSLSGVLKPDDTLKGYKGYVGLQGDEGPQGPT------------GEQGAVGRNGL 281
              ||.||.|...          |||.:|..|..|..||.|||            ||:|.   .|:
Mouse   220 --PGLKGNPATG----------LKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGV---KGM 269

  Fly   282 PGARGEIGGPGERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAG 346
            ||..|..|.||.:|.||       .|.||.||.||:.|.   .|.||..|..||.|..|:||.||
Mouse   270 PGMIGPPGPPGRKGAPG-------VGIKGEKGIPGFPGP---RGEPGSHGPPGFPGFKGIQGAAG 324

  Fly   347 PPGIYDPSLTKSLPGPIGSQGDIGPPGEQGPPG--------LPGKPGRRGPIGLAGQSGDPGLNG 403
            .||::         |.:|.:||:|..|..||||        |.|.||..||.|..|:.||.||.|
Mouse   325 EPGLF---------GFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPG 380

  Fly   404 SRGPPGRSERGEAGDYGFIGPPGPQGPPGEAGLPGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGY 468
            ..|||||.  ||... |.:|||||.|.||..|.||..|:   ||:....| |:||..|.|||.
Mouse   381 PPGPPGRP--GETCP-GMMGPPGPPGVPGPPGFPGEAGV---PGRLDCAP-GKPGKPGLPGLP-- 436

  Fly   469 RGDRGEVGLPGDKGLPGEGYNIVGPPGSQGPPGFRGLPGDDGYNGLRGLPGEKGLRGDDCPVCNA 533
                   |.||.:|.||... |...||..||.|.:|..|..|..|.:|..|.|||    | .|..
Mouse   437 -------GAPGPEGPPGSDV-IYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGL----C-TCPP 488

  Fly   534 GPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQGNPGRAGHKGLPGPAGIPGEPGKVGAAGP 598
            ||.||.|..|.   ||..|::|.:||.   |..|.:|:||:.|.:|.|||      ||:.||.||
Mouse   489 GPMGPPGPPGP---PGRQGSKGDLGLP---GWHGEKGDPGQPGAEGPPGP------PGRPGAMGP 541

  Fly   599 DGKAIEVGSLRKGEI-GDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDA-----GY 657
            .|...|.|.:....: |..|:.|..|..|..|..|:||.||..||||:.|.|||:.||     |.
Mouse   542 PGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGL 606

  Fly   658 QGRDGEPGRDGRDGAPGRNATTP--KVYLIGEP------GYDGIKGERGD--------------- 699
            .|..|.|||.|.:|.||.....|  :..|.|||      |:||.||::||
Mouse   607 PGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLP 671

  Fly   700 --DGDTGFKGVKGEPNPGQIYDNTGEPG---EDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGE 759
              ||..|.||..|.|         |.||   .||..|.:|..|..|..|..|.||::||.|..||
Mouse   672 GLDGPPGLKGFPGPP---------GAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGE 727

  Fly   760 VIPGPVGAKGYPGPTGDYGQQGAP-----GLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPP 819
            ....|:|..|.||..|..||:|.|     |.||..||.||.|..|.|||:|.||   ..|..|||
Mouse   728 KGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPG---CPGAGGPP 789

  Fly   820 GRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQP 884
            |..|..|..|..|..|..|..|.||..|...|:|..|..||.||||..|.     .|.||.||||
Mouse   790 GIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGD-----PGAPGWKGQP 849

  Fly   885 GRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGL------ 943
            |..|...|.|.||..|..|..|.:|..|..|..|.:|..||.|..||:||||.||.||.
Mouse   850 GDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGK 914

  Fly   944 PGMIGEIGERGEI---GYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKGETGNPG 1005
            ||..||.|.:||:   |:.|.:||:|.||..|:   :||.||.| :......|.||..|..|:.|
Mouse   915 PGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGD---RGLPGDAG-EAVTSRKGEPGDAGPPGDGG 975

  Fly  1006 FPGRPGAKGVAAYSGIKGDDGESGL-------------TGPIGYPGAPGAKGQRGPVGDSQPALD 1057
            |.|..|.||.:...|.:||.|..||             .||.|.||.||:.|.||.:|     ..
Mouse   976 FSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIG-----FP 1035

  Fly  1058 GVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQ-GRPGEPGAKGLGGYPGRNGINGLKGATG 1121
            |..|.:|:.|||||.|.||..|.:|.:|.:|.|..| |.||..|..|..||.||.|:.|.|   |
Mouse  1036 GFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEK---G 1097

  Fly  1122 FPGPQGPKGP---------------QGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEV 1171
            |||.:||:||               .|:.|:.||.|..|::||.||||..|:.|..|..|.:|.:
Mouse  1098 FPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKGPL 1162

  Fly  1172 GIPGRLENLRDRSFYRGFTGDQGLQGERGEQG-----DMGPIGFIGPPGAKGERGDIGYAGQLGF 1231
            |.|             |..|..||:||:|.:|     :|||.|.:|.||.|||:||.|..| :..
Mouse  1163 GSP-------------GLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKGDPGSPG-ISP 1213

  Fly  1232 DGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGH 1296
            .|..|.|||.|..|..|||           |.||..|||.:    |....|||||:.||.|..|.
Mouse  1214 PGLPGEKGFPGPPGRPGPP-----------GPAGAPGRAAK----GDIPDPGPPGDRGPPGPDGP 1263

  Fly  1297 RGPQIQGPP----------GPQGDVGFPGAPGHNGRHGLIGPKGELGDMGRQGERGESGYAIVGR 1351
            ||  :.|||          |..||.|.||.||..|..|..|.:|..|..|:.|::|..|..
Mouse  1264 RG--VPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLP---- 1322

  Fly  1352 QGDIGDIGFQGEPGWDGAKGEQGYPGLPGKNGRVGAPGPR---GPTGDA-GWGGIDGMDGLVGPK 1412
                   |..|.||..||.||:|.||.||:.|.||.||.|   ||..|. ....|.|:.|:.||:
Mouse  1323 -------GLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPGPR 1380

  Fly  1413 GQPGVTYSYSMARPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQ 1477
            |..|     :|..||.||.|| .|.:||.|..|.                  :|:.|..|:.||.
Mouse  1381 GPEG-----AMGEPGRRGLPG-PGCKGEPGPDGR------------------RGQDGIPGSPGPP 1421

  Fly  1478 GQRGDKGYMGLTGAPGLRGLPGPQGEPAPAPPAPKS-RGFIFARHSQSVHVPQCPANTNLLWEGY 1541
            |::||.|..|..|||   |.|||.|:|.|....|.| .||:...|||:...|.||.....||.||
Mouse  1422 GRKGDTGEAGCPGAP---GPPGPTGDPGPKGFGPGSLSGFLLVLHSQTDQEPACPVGMPRLWTGY 1483

  Fly  1542 SLSGNVAASRAVGQDLGQSGSCMMRFTTMPYMLCDITNVCHFAQNNDDSLWLSTAEPMPMTMTPI 1606
            ||.......:|..||||.:|||:..|:|:|:..|:|..|||:||.||.|.|||:|.|:|  |.|:
Mouse  1484 SLLYMEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLSSAAPLP--MMPL 1546

  Fly  1607 QGRDLMKYISRCVVCETTTRIIALHSQSMSIPDCPGGWEEMWTGYSYFMSTLDNVGGVGQNLVSP 1671
            ...::..|||||.|||...:.:|:|||..|||.||..|..:|.|||:.|.|.....|.||.|:||
Mouse  1547 SEEEIRSYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSP 1611

  Fly  1672 GSCLEEFRAQPVIECHG-HGRCNYYDALASFWLTVIEEQDQFVQ-PRQQTLKADFTS--KISRCT 1732
            |||||:|||.|.:||.| .|.|:::....|||||.:....||.. |...|||.....  |||||.
Mouse  1612 GSCLEDFRAAPFVECQGRQGTCHFFANEYSFWLTTVNPDLQFASGPSPDTLKEVQAQRRKISRCQ 1676

  Fly  1733 VCRR 1736
            ||.:
Mouse  1677 VCMK 1680

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	30/58 (52%)
		Collagen	102..152	CDD:189968	26/49 (53%)
		Collagen	277..334	CDD:189968	22/56 (39%)
		Collagen	534..593	CDD:189968	24/58 (41%)
		Collagen	814..871	CDD:189968	25/56 (45%)
		Collagen	957..1014	CDD:189968	22/56 (39%)
		Collagen	990..1049	CDD:189968	27/71 (38%)
		Collagen	1070..1128	CDD:189968	27/58 (47%)
		C4	1515..1624	CDD:128421	51/108 (47%)
		C4	1625..1737	CDD:128421	54/116 (47%)
Col4a4	NP_031761.1	7S domain. /evidence=ECO:0000250\|UniProtKB:P53420	31..56		5/12 (42%)
		Collagen	54..111	CDD:189968	29/59 (49%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	56..255		98/228 (43%)
		Triple-helical region. /evidence=ECO:0000250\|UniProtKB:P53420	57..1451		627/1577 (40%)
		Cell attachment site. /evidence=ECO:0000255	86..88		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	137..139		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	181..183		0/1 (0%)
		Collagen	291..348	CDD:189968	28/68 (41%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	379..1453		472/1205 (39%)
		Cell attachment site. /evidence=ECO:0000255	587..589		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	593..595		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	716..718		1/1 (100%)
		Collagen	805..858	CDD:189968	25/57 (44%)
		Collagen	889..948	CDD:189968	28/61 (46%)
		Cell attachment site. /evidence=ECO:0000255	980..982		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	992..994		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1144..1146		1/1 (100%)
		Collagen	1338..1405	CDD:189968	31/72 (43%)
		C4	1458..1562	CDD:279721	48/105 (46%)
		C4	1566..1679	CDD:279721	53/112 (47%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838355
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.740