DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ir25a and Grid2

DIOPT Version :9

Sequence 1:	NP_001260049.1	Gene:	Ir25a / 33683	FlyBaseID:	FBgn0031634	Length:	947	Species:	Drosophila melanogaster
Sequence 2:	NP_077355.2	Gene:	Grid2 / 79220	RGDID:	68368	Length:	1007	Species:	Rattus norvegicus

Alignment Length:	1017	Identity:	233/1017 - (22%)
Similarity:	417/1017 - (41%)	Gaps:	214/1017 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 LLGFLSLLSSFSLEIAAQTTQNINVLFINEVDNEPAAKAVEVVLTY---LKKNIRY----GLSVQ 71
            ||.|||...|.:.::|...    :::.|..:.:|.|.|..||..|.   |.:|...    .::..
  Rat     6 LLFFLSFWWSRTWDLATSD----SIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFS 66

  Fly    72 LDSIEANKSDAKVLLEAICNKYATSIEKKQTPHLILDTTKSGIASETVKSFTQALGLPTISASYG 136
            :..::.|.....|  :..|......|       |.|.::....::.:::|...|:.:|.:.....
  Rat    67 VTFVDGNNPFQAV--QEACELMNQGI-------LALVSSIGCTSAGSLQSLADAMHIPHLFIQRS 122

  Fly   137 QQGDLRQWRDLDEAKQK--YLLQVMPPADIIPEAIRSIVIHMNITNAAILYDDSFVMDHKYKSLL 199
            ..|..|....|..:.:.  |.|.|.||. .:.|.|..:|.........|.||..:.        :
  Rat   123 TAGTPRSGCGLTRSNRNDDYTLSVRPPV-YLNEVILRVVTEYAWQKFIIFYDSEYD--------I 178

  Fly   200 QNIQTRHVITAIAKDGKREREEQIEKLRNLDINNFFILGTLQSIRMVLESVKPAYFERNFA---- 260
            :.||  ..:..:::.|.....:::|...|..|...|....::.:....::::.|....|.|
  Rat   179 RGIQ--EFLDKVSQQGMDVALQKVENNINKMITTLFDTMRIEELNRYRDTLRRAILVMNPATAKS 241

  Fly   261 ----------------WHAITQNEGEISSQRDNATIMFMKPMAYTQYRDRLGLLRTTY----NLN 305
                            |..|.:...::..|.     :..:.:.      ||.::|.|:    |::
  Rat   242 FISEVVETNLVAFDCHWIIINEEINDVDVQE-----LVRRSIG------RLTIIRQTFPVPQNIS 295

  Fly   306 E-----------------EP-----QLSSAFYFDLALRSFLTIKEMLQSGAWPKDMEYLNC---- 344
            :                 :|     ::|:.:.:|..|.......:.|:...| ..|..|:|
  Rat   296 QRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKW-HSMASLSCIRKN 359

  Fly   345 -DDFQGGNTPQRNLDLRDYFTKITEPTSYGTFDLVTQSTQPFNGHSFMKFEMDINVLQIRGGSSV 408
             ..:|||.:....:. :.....:|....:|          ...|:..:.||    :|....|..:
  Rat   360 SKPWQGGRSMLETIK-KGGVNGLTGDLEFG----------ENGGNPNVHFE----ILGTNYGEEL 409

  Fly   409 NS--KSIGKW--ISGLNSELIVKDEEQMKNLTADTVYRIFTVVQAPFIMRDETA---PKGYKGYC 466
            ..  :.:|.|  ::|||..|   .:::::|.....|.|:.||::.||:|..|..   ||.|:|:.
  Rat   410 GRGVRKLGCWNPVTGLNGSL---TDKKLENNMRGVVLRVVTVLEEPFVMVSENVLGKPKKYQGFS 471

  Fly   467 IDLINEIAAIVHFDYTIQEVEDGKFGNMDENGQWNGIVKKLMDKQADIGLGSMSVMAEREIVIDF 531
            ||:::.::..:.|:|.|....|.|:|:..|:|.|||:|.:|:.|:||||:.::::..:||.|:||
  Rat   472 IDVLDALSNYLGFNYEIYVAPDHKYGSPQEDGTWNGLVGELVFKRADIGISALTITPDRENVVDF 536

  Fly   532 TVPYYDLVGITIMMQRPSSPSSLFKFLTVLETNVWLCILAAYFFTSFLMWIFDRWSPYSYQNNRE 596
            |..|.| ..:.::::|......:|..|...:.::|.||.........|:::.:..:|...|..
  Rat   537 TTRYMD-YSVGVLLRRAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVYLLNWLNPPRLQMG-- 598

  Fly   597 KYKDDEEKREFNLKECLWFCMTSLTPQGGGEAPKNLSGRLVAATWWLFGFIIIASYTANLAAFLT 661
                  ......|...:||...|...|||......|:.|::...||||..|:|:|||||||||||
  Rat   599 ------SMTSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFALIVISSYTANLAAFLT 657

  Fly   662 VSRLDTPVESLDDLAKQYKILYAPLNGSSAMTY--------FERMSNIEQMFYEIWKDLS-LNDS 717
            ::|:::.::||.||:||..|.|..:..|:...:        |||    :.|:.::|:.:: .|.|
  Rat   658 ITRIESSIQSLQDLSKQTDIPYGTVLDSAVYQHVRMKGLNPFER----DSMYSQMWRMINRSNGS 718

  Fly   718 LTAVERSKLAVWDYPVSDKYTKMWQAMQEAKLPATLDEAVARVRNSTAATGFAFLGDATDIRYLQ 782
            ...|..|:..:                |:.|.                 ..:||:.||..:.|:.
  Rat   719 ENNVLESQAGI----------------QKVKY-----------------GNYAFVWDAAVLEYVA 750

  Fly   783 LT--NCDLQVVGEEFSRKPYAIAVQQGSHLKDQFNNAILTLLNKRQLEKLKEKWW-KNDEALAKC 844
            :.  :|....||...:.:.|.||:|.||..:|.|:..||.|.....::.||.||| ||    .:|
  Rat   751 INDPDCSFYTVGNTVADRGYGIALQHGSPYRDVFSQRILELQQSGDMDILKHKWWPKN----GQC 811

  Fly   845 D-----KPEDQSDGISIQNIGGVFIVIFVGIGMACITLVFEYWWYRYRKNPRI--------IDVA 896
            |     ..:.:...:.|:::.|||.::..||.::|:..|.|.||.| ||..|:        ||:.
  Rat   812 DLYSSVDAKQKGGALDIKSLAGVFCILAAGIVLSCLIAVLETWWSR-RKGSRVPSKEDDKEIDLE 875

  Fly   897 EANAERSNAA----DHPGKLVDGVILGHSGEKFEKSKAALRP 934
            ..: .|.|:.    |.|.|            :|..|...|.|
  Rat   876 HLH-RRVNSLCTDDDSPHK------------QFSTSSIDLTP 904

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ir25a	NP_001260049.1	PBP1_iGluR_AMPA_Like	40..426	CDD:107378	75/449 (17%)
		PBP2_iGluR_putative	438..836	CDD:270435	120/412 (29%)
		Lig_chan	565..870	CDD:278489	85/321 (26%)
Grid2	NP_077355.2	Interaction with CBLN1 homotrimer. /evidence=ECO:0000250\|UniProtKB:O43424	24..345		55/355 (15%)
		Periplasmic_Binding_Protein_type1	28..429	CDD:415822	74/447 (17%)
		PBP2_iGluR_delta_2	440..806	CDD:270449	119/411 (29%)
		Interaction with AP4M1. /evidence=ECO:0000269\|PubMed:14572453	921..991
		PDZ-binding. /evidence=ECO:0000250	1005..1007
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166350001
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1052
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000021
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.740