Protein Alignment Ir25a and Ekar

DIOPT Version :9

• Sequence 1: NP_001260049.1
• Gene: Ir25a / 33683
• FlyBaseID: FBgn0031634
• Length: 947
• Species: Drosophila melanogaster
• Sequence 2: NP_001036250.1
• Gene: Ekar / 43806
• FlyBaseID: FBgn0039916
• Length: 899
• Species: Drosophila melanogaster
• Alignment Length: 966
• Identity: 244/966 - (25%)
• Similarity: 415/966 - (42%)
• Gaps: 232/966 - (24%)

  Fly    16 GFLSLLSSFSLEIAAQTTQNINVLFINEVDNEPAAKAVEVVLTY------LKKNIRYGLSVQLDS 74
            ||| |....|..:.......|..:|    .|:|.....|:...|      :.|::....:|....
  Fly     6 GFL-LFQFLSYGLGVPPLVRIGAIF----SNQPGMYNSELAFRYAIHRLNMDKSLLPETTVDYYV 65

  Fly    75 IEANKSDAKVLLEAICNKYATSIEKKQTPHLILDTTKSGIASETVKSFTQALGLPTISASYGQQG 139
            ...|:.|:...::.:|......::...:|      |.|.:|:. :.|...||.:|.|..|
  Fly    66 EYVNRFDSFETVQKVCKLIRVGVQAVFSP------TDSVLATH-INSICDALDIPNIGRS----- 118

  Fly   140 DLRQWRDLDEAKQKYLLQVMPPADIIPEAIRSIVIHMNITNAAILY--DDSFVMDHKY-KSLLQN 201
                       ...:.:.|.|...::..|...::.::|.|...||:  ::..:..|:. :|....
  Fly   119 -----------AHDFSINVYPSKQLVNYAFNDVIQYLNWTRFGILHEKENGIINLHQLSRSFHGE 172

  Fly   202 IQTRHVITAIAKDGKREREEQIEKL---RNLDINNFFILGTLQSIRMVLESV--------KPAY- 254
            :..|.|          .|:..:..|   :..:|:|..|......|.::|:::        |..|
  Fly   173 VHMRQV----------SRDSYVSALNEFKGKEIHNIIIDTNSNGISILLKNILQQQMNEYKYHYL 227

  Fly   255 -------------FERNFAWHAITQNEGEISSQR--DNATI---------------MFMKPMAYT 289
                         |:.||.         .|:|.|  |.|.:               :|.||..
  Fly   228 FTSFDLETYDLEDFKYNFV---------NITSFRLVDTADVGVKQILKDIGLYSHHIFKKPYL-- 281

  Fly   290 QYRDRLGLLRTTYNLNEEPQL--SSAFYFDLALRSF-----LTIKEML--QSGAWPKDM---EYL 342
                .|.:.::|. |..||.|  .|.:.|.:.|::.     ||:..:.  :..:|...:   .||
  Fly   282 ----NLHIKKSTI-LESEPALMFDSVYVFAIGLQTLEQSHSLTLLNISCEEENSWDGGLSLINYL 341

  Fly   343 NCDDFQG---------GNTPQRNLDL----RDYFTKITEPTSYGTFDLVTQSTQPFNGHSFMKFE 394
            |..:::|         |...|..|||    :....|:.|.|.:|..::    |:|     .|.|:
  Fly   342 NAVEWKGLTGPIQFKDGQRVQFKLDLIKLKQHSIVKVGEWTPHGHLNI----TEP-----SMFFD 397

  Fly   395 MDINVLQIRGGSSVNSKSIGKWISGLNSELIVKDEEQMKNLTADTVYRIFTVVQAPFIM----RD 455
                     .||             :|..|:|                 .|:::.|::|    ::
  Fly   398 ---------AGS-------------MNVTLVV-----------------ITILETPYVMMHYGKN 423

  Fly   456 ETAPKGYKGYCIDLINEIAAIVHFDYTIQEVEDGKFGNMD-ENGQWNGIVKKLMDKQADIGLGSM 519
            .|..:.:.|:|:|::..|:..|.|||.:..|.|.|:|..| |.|:|||:|.:||..:||:.:|||
  Fly   424 FTGNERFYGFCVDILETISREVGFDYILDLVPDRKYGAKDPETGEWNGMVAQLMKYKADLAVGSM 488

  Fly   520 SVMAEREIVIDFTVPYYDLVGITIMMQRPSS-PSSLFKFLTVLETNVWLCILAAYFFTSFLMWIF 583
            ::...||.|||||.|:.:| ||:|:.:.|:| |:.||.|:..|...:|:.:|.|||..|..::|.
  Fly   489 TITYARESVIDFTKPFMNL-GISILFKVPTSEPTRLFSFMNPLAIEIWIYVLIAYFLVSLCIYIV 552

  Fly   584 DRWSPYSYQ-NNREKYKDDEEKREFNLKECLWFCMTSLTPQGGGEAPKNLSGRLVAATWWLFGFI 647
            .:.||..:: .|....::.....:|:|.:..||.:.:...|.....|:.:|.|::::||..|..|
  Fly   553 GKLSPIEWKCINACDLENISIGNQFSLTDSFWFTIGTFMQQSPDIYPRAMSTRIISSTWGFFSLI 617

  Fly   648 IIASYTANLAAFLTVSRLDTPVESLDDLAKQYKILYAPLNGSSAMTYFERMSNIEQMFYEIWKDL 712
            |:||||||||||||..|:..|:|:.:|||.|.:|.|..|:..|.||:|
  Fly   618 IVASYTANLAAFLTTERMINPIENAEDLASQTEISYGTLDSGSTMTFF----------------- 665

  Fly   713 SLNDSLTAVERSKLAVWDYPVSDKYTKMWQAMQEAKLPA---TLDEAVARVRNSTAATGFAFLGD 774
              .||               |.:.|.|:|::|...|..|   |.::.:.||...    .:|||.:
  Fly   666 --RDS---------------VIETYKKIWRSMDNKKPSAFTTTYEDGIKRVNQG----NYAFLME 709

  Fly   775 ATDIRYLQLTNCDLQVVGEEFSRKPYAIAVQQGSHLKDQFNNAILTLLNKRQLEKLKEKWWKN-D 838
            :|.:.|:...:|:|..:|.....|.|.||..:||..:|:.:.|||.|..:..::.|.:||||| |
  Fly   710 STMLDYIVQRDCNLTQIGGLLDTKGYGIATPKGSPWRDKISLAILELQERGDIQMLYDKWWKNTD 774

  Fly   839 EALA-KCDKPEDQSDGISIQNIGGVFIVIFVGIGMACITLVFEYWW---YRYRKNP 890
            |... |....:.:::.:.:::|||||:|:..||.:|.:...||:|:   |.|...|
  Fly   775 ETCTRKNTSKQSKANSLGLESIGGVFVVLIAGIIVAAVVAFFEFWYNFRYNYEATP 830

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ir25a	NP_001260049.1	PBP1_iGluR_AMPA_Like	40..426	CDD:107378	84/461 (18%)
		PBP2_iGluR_putative	438..836	CDD:270435	132/407 (32%)
		Lig_chan	565..870	CDD:278489	95/310 (31%)
Ekar	NP_001036250.1	PBP1_iGluR_Kainate	24..388	CDD:107377	77/416 (19%)
		ANF_receptor	39..371	CDD:279440	69/380 (18%)
		PBP2_iGluR_Kainate	402..771	CDD:270432	135/424 (32%)
		Lig_chan	533..807	CDD:278489	95/311 (31%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45463128
Domainoid	1	1.000	82	0.746	Domainoid score	I2907
eggNOG	1	0.900	-	-		E1_KOG1052
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S6275
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000021
OrthoInspector	1	1.000	-	-		mtm1027
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR18966
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	8	7.790