Protein Alignment Ir25a and Grin2b

DIOPT Version :10

• Sequence 1: NP_001260049.1
• Gene: Ir25a / 33683
• FlyBaseID: FBgn0031634
• Length: 947
• Species: Drosophila melanogaster
• Sequence 2: NP_036706.1
• Gene: Grin2b / 24410
• RGDID: 2738
• Length: 1482
• Species: Rattus norvegicus
• Alignment Length: 995
• Identity: 223/995 - (22%)
• Similarity: 382/995 - (38%)
• Gaps: 268/995 - (26%)

  Fly     5 NPKTSKILWL-LGFLSLLSSFSLEIAAQTTQNINVLFINEVDNEPAAKAVEVVLTYLKKNIRYGL 68
            :||    .|| |..|::..|.:....:..:..|.|:.:...| |.|.|...      :|:..:.|
  Rat     9 SPK----FWLVLAVLAVSGSKARSQKSPPSIGIAVILVGTSD-EVAIKDAH------EKDDFHHL 62

  Fly    69 SV--QLDSIEANKSDAKVLLEAICNKYATSIEKKQTPHLILDTTKSGIASETVKSFTQALGLPTI 131
            ||  :::.:..|::|.|.::..||:  ..|..|.|......||.:..||.  :..|..|..|..|
  Rat    63 SVVPRVELVAMNETDPKSIITRICD--LMSDRKIQGVVFADDTDQEAIAQ--ILDFISAQTLTPI 123

  Fly   132 SASYGQQGDLRQWRDLDEAKQKY------------LLQVMPPADIIPEAIRSIVIHMNITNAAIL 184
            ...:|  |......|.||:...:            :|.:|...|..   |.|||     |.....
  Rat   124 LGIHG--GSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWY---IFSIV-----TTYFPG 178

  Fly   185 YDDSFVMDHKYKSLLQN------IQTRHVITAIAKDGKREREEQIEKLRNLDINNFFILGTLQSI 243
            |.| ||  :|.:|.::|      ::...::.....||..:.:.|::||::..|..:........|
  Rat   179 YQD-FV--NKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYI 240

  Fly   244 RMVLESVKPAYFERNFAW---HAITQNEGEISSQRDNATIMFMKPMAYTQY--------RDRLGL 297
            ..|..||....:  .:.|   ..:..:...:.|:.....|    .::|.::        ||.:.:
  Rat   241 FEVANSVGLTGY--GYTWIVPSLVAGDTDTVPSEFPTGLI----SVSYDEWDYGLPARVRDGIAI 299

  Fly   298 LRTTYNLNEEPQLSSAFYFDLALRSFLTIKEMLQSGAW---PKDMEYLNCDDFQGGNTPQRNL-- 357
            :.|                        ...:||...::   ||...|         ||.::.:
  Rat   300 ITT------------------------AASDMLSEHSFIPEPKSSCY---------NTHEKRIYQ 331

  Fly   358 --DLRDYFTKITEPTSYGTFDLVTQSTQPFNGH--SFMK--FEMDINVLQIRGGSSVNSKSIGKW 416
              .|..|...:|                 |.|.  ||.:  ::|...::.|........:.:|||
  Rat   332 SNMLNRYLINVT-----------------FEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKW 379

  Fly   417 ISGLNSELIVKDEE-QMKNLT-----------ADTVYRIFTVVQAPFIMRDETAP---------- 459
                      ||:. |||...           .|....|.|:.:|||::.:...|
  Rat   380 ----------KDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTV 434

  Fly   460 ---------------KGY-----KGYCIDLINEIAAIVHFDYTIQEVEDGKFGNMDENGQWNGIV 504
                           .||     ||:|||::.:|:..|.|.|.:..|.:||.|. ..||.|||::
  Rat   435 PCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGK-KINGTWNGMI 498

  Fly   505 KKLMDKQADIGLGSMSVMAEREIVIDFTVPYYDLVGITIMMQRPSSPSSLFKFLTVLETNVWLCI 569
            .:::.|:|.:.:||:::..||..|:||:||:.: .||::|:.|.:...|...||.....:||:.:
  Rat   499 GEVVMKRAYMAVGSLTINEERSEVVDFSVPFIE-TGISVMVSRSNGTVSPSAFLEPFSADVWVMM 562

  Fly   570 LAAYFFTSFL-MWIFDRWSPYSYQNNREKYKDDEEK--REFNLKECLWFCM-----TSLTPQGGG 626
            .......|.: :::|:.:||..|  || ...|..|.  ..|.:.:.:|...     .|:..|.
  Rat   563 FVMLLIVSAVAVFVFEYFSPVGY--NR-CLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQN-- 622

  Fly   627 EAPKNLSGRLVAATWWLFGFIIIASYTANLAAFLTVSRLDTPVESLDDLAKQYKILYAP------ 685
              ||..:.:::.:.|..|..|.:||||||||||:........|..|.|...|....::|
  Rat   623 --PKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGT 685

  Fly   686 -LNGSSAMTYFERMSNIEQMFYEI------WKDLSLNDSLTAVERSKLAVWDYPVSDKYTKMWQA 743
             .|||:     ||  ||...:.|:      :....::|:|.:::..||.
  Rat   686 VPNGST-----ER--NIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLD---------------- 727

  Fly   744 MQEAKLPATLDEAVARVRNSTAATGFAFLGDATDIRYL--QLTNCDLQVV--GEEFSRKPYAIAV 804
                                      ||:.||..:.|:  :...|.|..:  |:.|:...|.||:
  Rat   728 --------------------------AFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAI 766

  Fly   805 QQGSHLKDQFNNAILTLLNKRQLEKLKEKWWKNDEALAKCDKPEDQSDGISIQNIGGVFIVIFVG 869
            |:.|..|.|.:.|||.|....::|:|:..|.   ..:...:|.|..|..:.|.|:.|||.::...
  Rat   767 QKDSGWKRQVDLAILQLFGDGEMEELEALWL---TGICHNEKNEVMSSQLDIDNMAGVFYMLGAA 828

  Fly   870 IGMACITLVFE---YWWYRY 886
            :.::.||.:.|   ||.:|:
  Rat   829 MALSLITFICEHLFYWQFRH 848

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ir25a	NP_001260049.1	PBP1_iGluR_AMPA_Like	37..418	CDD:380606	81/422 (19%)
		PBP2_iGluR_putative	438..836	CDD:270435	115/452 (25%)
Grin2b	NP_036706.1	PBP1_iGluR_NMDA_NR2	33..388	CDD:380601	86/444 (19%)
		PBP2_iGluR_NMDA_Nr2	403..803	CDD:270436	115/460 (25%)
		Pore-forming. /evidence=ECO:0000269|PubMed:24876489, ECO:0000305|PubMed:24876489	604..623		3/22 (14%)
		NMDAR2_C	840..1482	CDD:463148	3/9 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1161..1194
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1269..1301
		Interaction with DAPK1. /evidence=ECO:0000250|UniProtKB:Q01097	1292..1304
		PDZ-binding	1480..1482