DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG3225 and Dhx36

DIOPT Version :9

Sequence 1:	NP_608860.1	Gene:	CG3225 / 33680	FlyBaseID:	FBgn0031631	Length:	678	Species:	Drosophila melanogaster
Sequence 2:	NP_001101148.1	Gene:	Dhx36 / 310461	RGDID:	1308767	Length:	1000	Species:	Rattus norvegicus

Alignment Length:	768	Identity:	218/768 - (28%)
Similarity:	362/768 - (47%)	Gaps:	131/768 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 ITDSAGGEQQSSAFVFNVNHNMGLMEQ--------------------RERLPIRQYRDQILYCLE 61
            |||.:..::.|...:.....|:||.:|                    |::||....:.:::..:.
  Rat   149 ITDKSYIDRDSEYLLQQNEPNLGLDQQLLEDLQKKKTDPRYIEMQRFRKKLPSYGMQKELVNLIN 213

  Fly    62 KHQVVILVGETGSGKSTQVPQYLYEWGWHTKGL-----IGITEPRRVSTVTLANRVAQERGELV- 120
            .|||.::.||||.||:|||.|::.: .:..:|:     |..|:|||:|.:::|.|||.||.|..
  Rat   214 NHQVTVISGETGCGKTTQVTQFILD-NYIERGIGSACRIVCTQPRRISAISVAERVAAERAESCG 277

  Fly   121 -GDTVGYVVRFLESM-SSCTKIKFMTEGILLREVLADPLLTQYGVIIVDEAHERNMLTDMILGLL 183
             |::.||.:|....: .....|.:.|.||:|:.:.:|..|:....|::||.||||:.:|:::.::
  Rat   278 NGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVI 342

  Fly   184 KKILRKRSSLKLIISSATIDASFFSEFFS------WPGSGEVSVKLSIEGRMHPVSNF------- 235
            |.:|..||.||:|:.|||::|..|||:|.      .||.....|:..:|..:..:..|
  Rat   343 KDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYFPEQKEHR 407

  Fly   236 ------------------------------YLNEPCADYVKETVETVWKLHQ------------- 257
                                          |:.|....|...|::.:..:..
  Rat   408 SQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELQTRYSASTIDVLEMMDDDKVDLNLIAALIR 472

  Fly   258 ----KEPPGDILAFLTGQEEVLEALDLLREYIASSEQENLKVLPMYGSMSSTDQLSVFFTPPKGT 318
                :|..|.||.||.|.:.:....|||...: ..:.:...::|::..|.:.:|..||...|.|.
  Rat   473 YIVLEEEDGAILVFLPGWDNISTLHDLLMSQV-MFKSDRFLIIPLHSLMPTVNQTQVFKKTPPGV 536

  Fly   319 RKVVLATNIAETSITIPGIVYVIDCGYVKVKWYNPKTCSDSLVIVPVSKASAIQRAGRAGRMRPG 383
            ||:|:|||||||||||..:|||||.|.:|...::.:....::....||||:|.||.|||||::||
  Rat   537 RKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPG 601

  Fly   384 KVYRLYTKSDYEALAPRQPPEMRRSELSGAILQLKALGIGNILRF--DFPSPPPAQNLLSALESL 446
            ..|.||.......|...|.||:.|:.|....||:|.|.:|.|..|  ....||..:.::.:::.|
  Rat   602 HCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSDEAVVLSIKHL 666

  Fly   447 FALDAIDEQGNLTKPVGYLLAELPFSAMLSKMLYVSGQMGCSEEIITIIALLQVQSIFSRPASAV 511
            ..|.|:|:|..|| |:|..||.||....:.||:.......|.:.::||.|.|..:..|..|   :
  Rat   667 MELSALDKQEELT-PLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIP---L 727

  Fly   512 AQQSGRIAHRKFEVAE---GDFITMLNAYTGFVE---EGM--TKEFCGQYFLIYRNLKRAHSLRE 568
            .::....|.|| |:|:   .|.:|::||:.|:.|   .|.  .|::|.:|||....|:..|:::.
  Rat   728 GKEKIADARRK-ELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKG 791

  Fly   569 QLIT-------VARKKYGIPIFSCKGDVEKLCKC-ITAGFFTQVAYLH-HSGVYRQI------SS 618
            |...       |:.:....|..:...|.||:.|. |.||.:.:||.:. :.|..|::      |.
  Rat   792 QFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSD 856

  Fly   619 GTELAIHPNSTLYTLPQAQY--VVYGELLQTTKLFMNYVTVIKREWLTELAPH 669
            |. ::|||.|.........|  ::|...::|:.:::..        .||::|:
  Rat   857 GL-VSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYD--------CTEVSPY 900

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG3225	NP_608860.1	HrpA	40..668	CDD:224557	211/742 (28%)
		DEXDc	64..202	CDD:238005	54/145 (37%)
		HELICc	289..380	CDD:197757	39/90 (43%)
		HA2	448..534	CDD:214852	28/88 (32%)
		OB_NTP_bind	<581..670	CDD:285018	24/99 (24%)
Dhx36	NP_001101148.1	HrpA	187..893	CDD:224557	207/713 (29%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	257..309		18/51 (35%)
		DEAH box. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00541	326..329		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250\|UniProtKB:Q05B79	379..620		65/241 (27%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	490..549		19/59 (32%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	509..520		1/10 (10%)
		WH domain. /evidence=ECO:0000250\|UniProtKB:Q05B79	621..690		25/69 (36%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	630..689		20/59 (34%)
		OB-fold-like subdomains. /evidence=ECO:0000250\|UniProtKB:Q05B79	833..897		14/72 (19%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	841..852		2/10 (20%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250\|UniProtKB:Q05B79	862..892		6/29 (21%)
		Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..192		8/42 (19%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000269\|PubMed:23651854	1..96
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..53
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	1..43
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000250\|UniProtKB:Q9H2U1	45..97
		DSM (DHX36-specific motif). /evidence=ECO:0000250\|UniProtKB:Q05B79, ECO:0000250\|UniProtKB:Q9H2U1	45..67
		RecA-like domain 1. /evidence=ECO:0000250\|UniProtKB:Q05B79	98..378		72/229 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1643
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53574
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.820