Protein Alignment CG3225 and DHX9

DIOPT Version :10

• Sequence 1: NP_608860.1
• Gene: CG3225 / 33680
• FlyBaseID: FBgn0031631
• Length: 678
• Species: Drosophila melanogaster
• Sequence 2: NP_001348.2
• Gene: DHX9 / 1660
• HGNCID: 2750
• Length: 1270
• Species: Homo sapiens
• Alignment Length: 723
• Identity: 213/723 - (29%)
• Similarity: 339/723 - (46%)
• Gaps: 134/723 - (18%)

  Fly    35 NHNM-GLMEQRERLPIRQYRDQILYCLEKHQVVILVGETGSGKSTQVPQY-LYEWGWHTKGL--- 94
            :|:: .::::||.||::::..:||..:.::.|||:.|.||.||:|||||: |.::..:.:..
Human   375 DHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECN 439

  Fly    95 IGITEPRRVSTVTLANRVAQERGELVGDTVGYVVRFLESM--SSCTKIKFMTEGILLREVLADPL 157
            |.:|:|||:|.|::|.|||.||||..|.:.||.||| ||:  .....|.|.|.|:|||::.|
Human   440 IVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRF-ESILPRPHASIMFCTVGVLLRKLEA--- 500

  Fly   158 LTQYGV-----IIVDEAHERNMLTDMILGLLKKILRKRSSLKLIISSATIDASFFSE-FFSWPGS 216
                |:     :||||.|||::.||.:|.:|:.:::....:::::.|||||.|.|.| ||:.|
Human   501 ----GIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCP-- 559

  Fly   217 GEVSVKLSIEGRMHPVSNFYL---------------------------------NEPCAD-YVKE 247
                 .:.:.||.:||..::|                                 |..|.| |..|
Human   560 -----IIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPE 619

  Fly   248 TVETVWKLHQKEP-----------------PGDILAFLTG------QEEVLEALDLLREYIASSE 289
            |..::.:|::||.                 ||.:|.||.|      .::.||    :..:..|
Human   620 TRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLE----MNPHFGS-- 678

  Fly   290 QENLKVLPMYGSMSSTDQLSVFFTPPKGTRKVVLATNIAETSITIPGIVYVIDCGYVKVKWYNPK 354
             ...::||::..:...:|..||...|.|..||:|:||||||||||..:|||||....|||.:...
Human   679 -HRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAH 742

  Fly   355 TCSDSLVIVPVSKASAIQRAGRAGRMRPGKVYRLYTKSDYEALAPRQPPEMRRSELSGAILQLKA 419
            ....:...|..||.:..||.|||||:|||..:.|.:::.:|.|.....|||.|:.|....|.:|.
Human   743 NNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKL 807

  Fly   420 LGIGNILRF--DFPSPPPAQNLLSALESLFALDAIDEQGNLTKPVGYLLAELPFSAMLSKMLYVS 482
            |.:|.|.:|  ....|||...::.|..:|..|||:|....|| |:|.:||:||......||:.
Human   808 LRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELT-PLGRILAKLPIEPRFGKMMI-- 869

  Fly   483 GQMGCSEEIITIIALLQVQSIFSRPASAVAQQSGRIAHRKFEVAE-GDFITMLNAYTGFVEEGMT 546
              |||...:...|..:...:.|..|.....::.|.| ||.|.... .|.:.:|:.:..:.:..|.
Human   870 --MGCIFYVGDAICTIAAATCFPEPFINEGKRLGYI-HRNFAGNRFSDHVALLSVFQAWDDARMG 931

  Fly   547 KE-----FCGQYFLIYRNLKRAHSLREQLITVARKKYGIP-------IFSCKG---DVEKLCKCI 596
            .|     ||....|....|:.....:.||..:.... |.|       :|:..|   :::.:...:
Human   932 GEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINS-GFPEDCLLTQVFTNTGPDNNLDVVISLL 995

  Fly   597 TAGFFTQVAYLHHSGVYRQI--SSGTELAIHPNST---------LYTLPQAQYVVYGELLQTTKL 650
            ..|.:..|.|....   |:|  :.|....||.:|.         .|..|   :.|:||.::|..:
Human   996 AFGVYPNVCYHKEK---RKILTTEGRNALIHKSSVNCPFSSQDMKYPSP---FFVFGEKIRTRAI 1054

  Fly   651 FMNYVTVI 658
            ....:|::
Human  1055 SAKGMTLV 1062

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG3225	NP_608860.1	HrpA	38..>669	CDD:441249	212/720 (29%)
DHX9	NP_001348.2	Interaction with CREBBP. /evidence=ECO:0000269|PubMed:9323138	1..250
		DSRM_DHX9_rpt1	3..71	CDD:380683
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYY	5..9
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYY	53..55
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	83..118
		DSRM_DHX9_rpt2	178..252	CDD:380684
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYX	182..186
		Interaction with BRCA1. /evidence=ECO:0000269|PubMed:9662397	230..325
		siRNA-binding. /evidence=ECO:0000305|PubMed:23361462	234..236
		Necessary for interaction with RNA polymerase II holoenzyme. /evidence=ECO:0000269|PubMed:9323138	255..664		95/303 (31%)
		Necessary for interaction with H2AX. /evidence=ECO:0000269|PubMed:15613478	313..952		191/604 (32%)
		DEXHc_DHX9	330..563	CDD:350730	75/202 (37%)
		MTAD. /evidence=ECO:0000269|PubMed:11416126	331..380		1/4 (25%)
		HrpA	388..>886	CDD:441249	173/524 (33%)
		Core helicase. /evidence=ECO:0000305|PubMed:25062910	398..809		143/432 (33%)
		DEIH box	511..514		2/2 (100%)
		Nuclear localization signal (NLS1). /evidence=ECO:0000255	586..595		0/8 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	588..608		0/19 (0%)
		HA2. /evidence=ECO:0000269|PubMed:25062910	831..919		28/93 (30%)
		OB-fold. /evidence=ECO:0000269|PubMed:20696886, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:25149208	958..1074		22/112 (20%)
		OB_NTP_bind	994..1073	CDD:400182	16/75 (21%)
		RGG. /evidence=ECO:0000269|PubMed:10207077, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:25149208, ECO:0000269|PubMed:9111062	1150..1270
		Nuclear localization signal (NLS2). /evidence=ECO:0000269|PubMed:16375861	1155..1173
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1229..1248