Protein Alignment ed and nphs1

DIOPT Version :10

• Sequence 1: NP_523469.2
• Gene: ed / 33619
• FlyBaseID: FBgn0000547
• Length: 1332
• Species: Drosophila melanogaster
• Sequence 2: XP_073779348.1
• Gene: nphs1 / 692352
• ZFINID: ZDB-GENE-051102-1
• Length: 1287
• Species: Danio rerio
• Alignment Length: 1291
• Identity: 247/1291 - (19%)
• Similarity: 428/1291 - (33%)
• Gaps: 426/1291 - (32%)

  Fly    45 SIDTRENADLTLKCRFNDKYEANDFSFFWTRWTANPAQFDNVAIGEVQLSSG---YRLDFQPERG 106
            ::..|..|...|||.......|       .:|..     |.:.:|.::...|   |.:....::|
Zfish    80 NVTVRAGATALLKCEVLRASGA-------VQWVK-----DGLLLGPLRSLPGYPRYSMTGDQKKG 132

  Fly   107 IYDLQIKNVSYNRDNGRFECRIKAKGTGADVHQEFHNLTVLTPPHPPVISPGNIAVATEDKPMEL 171
            .|.|||.:|:. .|:|.:||::....:...:......|.|..|...|.....:.....|.:...:
Zfish   133 QYHLQILDVTL-EDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESEEPWVEGEQYTV 196

  Fly   172 TCSSIGGSPDPTITWYREGSNTPLPATVLKGGTKD--QPTNATLSIIPRREDDGAKYKCVVRNRA 234
            ||.:....|...||.:|:|.......:....|::|  |.|:|.::|..|..||..:..|..:|.|
Zfish   197 TCIAPDAKPPAEITMFRDGEELLESESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPA 261

  Fly   235 MNEGKRLEATATLNVNYYPRVE--VGPENPLRVERDRTAKLECNVDAKPKVPNVRWNRNGRFISS 297
              ..:.||...|:.|.:.|:..  :|.||. .|:.....|:.|.......:..:.|.:||..:|:
Zfish   262 --SPRALETGMTMKVYFPPQAPMIMGLENE-EVKAGSFLKVVCMSYGGNPLATLHWTKNGEVLST 323

  Fly   298 S--------LVHTIHRVSVQDAGKYTCIADNGLGKTGEQEL----ILDILYPPMVVIESKTREAE 350
            |        ...::.::.|:....:..:....:.:.....:    .|.:|:.|..|....:.||.
Zfish   324 SWDVDFVSRRASSVLKMEVKPEDNHAVLQCQSINQVTRSPMSLTRTLTVLFEPAEVTLLGSFEAV 388

  Fly   351 EGDTVTIRCNVTANPAPVTIEW---LKE-NSPDFRY----NGDVLTLTSV-----RADHAGNYIC 402
            ||:.:.:.|:.:::..||.|.|   .|| |..:..:    ||.::|::::     |.|:..:..|
Zfish   389 EGEEINLSCSTSSSNPPVHIRWWLGFKELNRTEVTFAEGDNGGMMTVSNLTHKVSREDNGLSLQC 453

  Fly   403 RAVNIMQSQGMERSERVGNSTVA-LLVRHRPGQAYITPNKPVV--HVGNGVTLTC---SANPPGW 461
            .|.|        :..|..:...| |:|.:.|.:.::....|.|  |.|:.:.|.|   ..||.|
Zfish   454 EAFN--------KGTRFSSVRAAELIVYYPPQKVWLDAPPPNVFLHSGSTLRLVCFSSGGNPTG- 509

  Fly   462 PVPQYRWFR-------------------------------------------------------- 470
               |..|.:
Zfish   510 ---QLTWLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKFRVL 571

  Fly   471 ---------------------DMD---GEFSSTQKI-------LAQGPQYSIPKAHLGNEGK--- 501
                                 |:|   |..:.|..|       :.:|...:..:|..|...:
Zfish   572 FPAVSVKIVAKQKELRRGQTLDLDCLAGSSNPTANISWSLGPVILKGVDQAPKRAEFGGMSRTSK 636

  Fly   502 -------YH------CHAV-NELGIGMMATIVLEIHQPPQFLAKLQQHMTRRVADTDYTVTCSAK 552
                   :|      |.|. |.|..|:.....|::..||:|..:....:  :|.: |.|.|..||
Zfish   637 LSLTLESHHWRKRITCQAFSNVLSEGVNNFYSLDVLFPPEFSDEQPDEV--QVIE-DETATLPAK 698

  Fly   553 GKPAP---SVKWLKDAVEILPEENL---------------------YEVQTNPDQGLNG---MVT 590
            ....|   :.:|:....:::.|.:.                     |.::.:..:|.|.   .:.
Zfish   699 VSANPDEITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLD 763

  Fly   591 VQSQLKFRGKARP--------------------------------------------NGNAL--- 608
            ||.....|.|:.|                                            :|..:
Zfish   764 VQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEESEDDGTGVLTI 828

  Fly   609 --VPGDR-GLYTCLYQNEVNSANS-SMQLRIEHEPIVLH--QYNKVAF--DIRETAEVVCKVQAY 665
              |..|| |:|.|...|.::...| ..||.:...|.:..  |:.|||.  |....|.|||:.|..
Zfish   829 YEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGV 893

  Fly   666 PKPEFQWQFGNNPSPLTMSSDGHYEISTTTDNNDIYTSVLK-INSLTHSDYGEYTCRVANTLDTI 729
            |:.:|:|  ..|..||.:.:..:.|  ||.....|:||:|. ||.....||..:||...|::...
Zfish   894 PRVQFRW--AKNGFPLDLGNPRYEE--TTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGED 954

  Fly   730 RAPIRLQQKGPPEKPTNLRATEVGHNYVSLSWDPGFDGGLSKTKFF---------VSYRRVAMPR 785
            ...|:|.....|:.|::|:...|....|:|.|.|||||||  |:.|         .||..|
Zfish   955 SLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGL--TQIFRVRYQWAGSASYMYV---- 1013

  Fly   786 EEQLIPDCATLANSNSAWVEVDCQRDIPCKVTALEQHQSYAFKVKALNPKSDSPYSSEIMVTTKV 850
              .:.|..||:..                 ||.|.....|.|.|.|||...:|.|:.
Zfish  1014 --DVFPPTATVYT-----------------VTGLNPSTMYNFSVNALNSIGESSYAD-------- 1051

  Fly   851 SRIPPPLQVTYEPNTRTLGIDVGATCLNLVAVVESMVNADSPMAAWEVVTTMDNLQLSGNGPTHK 915
                         |.:.|                             :|||.|
Zfish  1052 -------------NNKIL-----------------------------MVTTAD------------ 1062

  Fly   916 EKIIDRIIGARRVGGGRALGHTISEDEDDNGLNSLALEDENSPTVRVKLCLRTNPEHCGDYTDAE 980
                     |:...||      :|..:.|:.|
Zfish  1063 ---------AKEADGG------VSTADQDSAL--------------------------------- 1079

  Fly   981 IGRPYIAEANALATPTLIAIIVSCVVFALFAGLILMFCRCKRNQSKK---SAAAKDYEMDSVRPS 1042
                   ....|....::.::|..|:|.|...:..:..|.||.:|.|   |:.:::.:.:..|.:
Zfish  1080 -------AGGGLPVYVVVILVVGGVLFLLNPFICFLLARWKRRRSLKGDTSSISQETKSEEQRST 1137

  Fly  1043 IVAAQQNQAPPPYYPASGLDNKALEHSMDLALSMEDQKTALYAT----QNGYSYHP 1094
            ..::.:       |.:..|.|.:.:.:: |..|..:..:::|.:    :|.:.|:|
Zfish  1138 ASSSTR-------YASRELINASAQRTL-LVDSSSEPDSSVYESYGRQENPHYYYP 1185

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ed	NP_523469.2	V-set	50..147	CDD:462230	21/99 (21%)
		Ig	153..249	CDD:472250	24/97 (25%)
		Ig strand B	169..173	CDD:409353	0/3 (0%)
		Ig strand C	183..187	CDD:409353	2/3 (67%)
		Ig strand E	211..215	CDD:409353	1/3 (33%)
		Ig strand F	225..230	CDD:409353	1/4 (25%)
		Ig strand G	242..245	CDD:409353	1/2 (50%)
		Ig_3	253..320	CDD:464046	13/76 (17%)
		Ig_3	337..406	CDD:464046	21/81 (26%)
		Ig_2	437..>504	CDD:464026	20/174 (11%)
		Ig	525..635	CDD:472250	31/187 (17%)
		Ig strand B	545..549	CDD:409568	1/3 (33%)
		Ig strand C	558..562	CDD:409568	0/3 (0%)
		Ig strand E	587..591	CDD:409568	0/6 (0%)
		Ig strand F	615..620	CDD:409568	2/4 (50%)
		Ig strand G	628..631	CDD:409568	1/3 (33%)
		Ig_3	639..724	CDD:464046	30/89 (34%)
		FN3	741..848	CDD:238020	33/115 (29%)
nphs1	XP_073779348.1	None