DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Shaw and Kcnb1

DIOPT Version :9

Sequence 1:	NP_001137782.1	Gene:	Shaw / 33599	FlyBaseID:	FBgn0003386	Length:	619	Species:	Drosophila melanogaster
Sequence 2:	NP_037318.2	Gene:	Kcnb1 / 25736	RGDID:	2954	Length:	857	Species:	Rattus norvegicus

Alignment Length:	672	Identity:	227/672 - (33%)
Similarity:	325/672 - (48%)	Gaps:	131/672 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 RVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPIL----------NEYFFDRHPGVFAQV 80
            ||.|||||:.||....||.::|.|||.:|.:. ..:|.:|          ||||||||||.|..:
  Rat    32 RVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDC-NTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSI 95

  Fly    81 LNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKP 145
            ||:||||:||...::|...|.:||::||:|...:|.||...|.|.::           .::.|..
  Rat    96 LNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKE-----------QMNEELK 149

  Fly   146 SEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKT 210
            .|.|..|:...||  :..|..  .|.:.::|.|.::|.||.|||.:.::|:.||.:|.::..|.|
  Rat   150 REAETLREREGEE--FDNTCC--AEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNT 210

  Fly   211 HPDMRVPIVRNITVKTANGSNGWFLD---KTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKW 272
            .|:::                  .||   ::..|..:|  ::|.||.||||.|.|:||:|||.||
  Rat   211 LPELQ------------------SLDEFGQSTDNPQLA--HVEAVCIAWFTMEYLLRFLSSPKKW 255

  Fly   273 EFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEF---------FSIIRIMRLFKLTRHSS 328
            :|.|..:|.||.:|.|.:|:.:.|..     .|..:|:|         |.|:||:|:.||.|||:
  Rat   256 KFFKGPLNAIDLLAILPYYVTIFLTE-----SNKSVLQFQNVRRVVQIFRIMRILRILKLARHST 315

  Fly   329 GLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTV 393
            ||:.|..|.|.|..||.||:.||.:||:||:|||::||:.:.:  ..|.|||...|||.:|||||
  Rat   316 GLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDD--TKFKSIPASFWWATITMTTV 378

  Fly   394 GYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPR 458
            ||||:.|||.:|..||.||.:||||.||||:|:||:||:.:|.. |.|.:...|||..|  |:.:
  Rat   379 GYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKE-QKRQEKAIKRREAL--ERAK 440

  Fly   459 QPRLPGAPGGVSGCGTPGSGPHSGPM-----GSGGTGPRRMN---------NK---TKDLVSPKS 506
            :      .|.:.......:...|..|     ...|....:.:         ||   ||..:|..|
  Rat   441 R------NGSIVSMNMKDAFARSIEMMDIVVEKNGESIAKKDKVQDNHLSPNKWKWTKRALSETS 499

  Fly   507 VAQLF-AGPLGASIVAMSPRTMLDLNPA------LAMGKPTFQPRIPT----PLAATP------- 553
            .::.| ....|:...|.|..:...||..      ..|.|...||.:.|    |.:..|
  Rat   500 SSKSFETKEQGSPEKARSSSSPQHLNVQQLEDMYSKMAKTQSQPILNTKEMAPQSKPPEELEMSS 564

  Fly   554 -PPPVSSAGGMTASGIGTTSATG-------ATSAPQ-------PATPLPSIAVSTTA-------S 596
             |.||:.....|...|...|.:.       ||..|:       |...|.|.|.|:||       :
  Rat   565 MPSPVAPLPARTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSSKAGSSTAPEVGWRGA 629

  Fly   597 VGKDLGISTTTTTAQETSKKAF 618
            :|...|..|.|....|||:..|
  Rat   630 LGASGGRLTETNPIPETSRSGF 651

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Shaw	NP_001137782.1	BTB	27..125	CDD:197585	47/107 (44%)
		BTB_2	27..117	CDD:280393	44/99 (44%)
		Ion_trans	244..439	CDD:278921	98/203 (48%)
		Ion_trans_2	355..429	CDD:285168	42/73 (58%)
Kcnb1	NP_037318.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		BTB_POZ_KCNB2	15..141	CDD:349719	49/109 (45%)
		Self-association. /evidence=ECO:0000269\|PubMed:18463252, ECO:0000269\|PubMed:19690160	59..75		3/16 (19%)
		Ion_trans	188..424	CDD:395416	110/263 (42%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	377..382		4/4 (100%)
		Self-association. /evidence=ECO:0000269\|PubMed:18463252, ECO:0000269\|PubMed:19690160	448..481		3/32 (9%)
		Kv2channel	467..678	CDD:397541	44/185 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	476..524		10/47 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	540..569		6/28 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	610..658		14/42 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	770..802
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..857
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X19
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.810