DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Shaw and Kcnb1

DIOPT Version :9

Sequence 1:	NP_001137782.1	Gene:	Shaw / 33599	FlyBaseID:	FBgn0003386	Length:	619	Species:	Drosophila melanogaster
Sequence 2:	NP_032446.2	Gene:	Kcnb1 / 16500	MGIID:	96666	Length:	857	Species:	Mus musculus

Alignment Length:	670	Identity:	220/670 - (32%)
Similarity:	321/670 - (47%)	Gaps:	155/670 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 RVVLNVGGIRHETYKATLKKIPATRLSRLTEALANYDPIL----------NEYFFDRHPGVFAQV 80
            ||.|||||:.||....||.::|.|||.:|.:. ..:|.:|          ||||||||||.|..:
Mouse    32 RVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDC-NTHDSLLQVCDDYSLEDNEYFFDRHPGAFTSI 95

  Fly    81 LNYYRTGKLHYPTDVCGPLFEEELEFWGLDSNQVEPCCWMTYTQHRDTQETLAVLDRLDLDTEKP 145
            ||:||||:||...::|...|.:||::||:|...:|.||...|.|.::           .::.|..
Mouse    96 LNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKE-----------QMNEELK 149

  Fly   146 SEEELARKFGFEEDYYKGTISWWQEMKPRIWSLFDEPYSSNAAKTIGVVSVFFICISILSFCLKT 210
            .|.|..|:...||  :..|..  .|.:.::|.|.::|.||.|||.:.::|:.||.:|.::..|.|
Mouse   150 REAETLREREGEE--FDNTCC--AEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNT 210

  Fly   211 HPDMRVPIVRNITVKTANGSNGWFLD---KTQTNAHIAFFYIECVCNAWFTFEILVRFISSPNKW 272
            .|:::                  .||   ::..|..:|  ::|.||.||||.|.|:||:|||.||
Mouse   211 LPELQ------------------SLDEFGQSTDNPQLA--HVEAVCIAWFTMEYLLRFLSSPKKW 255

  Fly   273 EFIKSSVNIIDYIATLSFYIDLVLQRFASHLENADILEF---------FSIIRIMRLFKLTRHSS 328
            :|.|..:|.||.:|.|.:|:.:.|..     .|..:|:|         |.|:||:|:.||.|||:
Mouse   256 KFFKGPLNAIDLLAILPYYVTIFLTE-----SNKSVLQFQNVRRVVQIFRIMRILRILKLARHST 315

  Fly   329 GLKILIQTFRASAKELTLLVFFLVLGIVIFASLVYYAERIQPNPHNDFNSIPLGLWWALVTMTTV 393
            ||:.|..|.|.|..||.||:.||.:||:||:|||::||:.:.:  ..|.|||...|||.:|||||
Mouse   316 GLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDD--TKFKSIPASFWWATITMTTV 378

  Fly   394 GYGDMAPKTYIGMFVGALCALAGVLTIALPVPVIVSNFAMYYSHTQARAKLPKKRRRVLPVEQPR 458
            ||||:.|||.:|..||.||.:||||.||||:|:||:||:.:|.. |.|.:...|||..|  |:.:
Mouse   379 GYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKE-QKRQEKAIKRREAL--ERAK 440

  Fly   459 QPRLPGAPGGVSGCGTPGSGPHSGPM-----GSGGTGPRRMN---------NK---TKDLVSPKS 506
            :      .|.:.......:...|..|     ...|.|..:.:         ||   ||..:|..|
Mouse   441 R------NGSIVSMNMKDAFARSIEMMDIVVEKNGEGVAKKDKVQDNHLSPNKWKWTKRALSETS 499

  Fly   507 VAQLF-AGPLGASIVAMSPRTMLDLN-----------------PALAMGK--PTFQPR------- 544
            .::.| ....|:...|.|..:...||                 |.|...:  |..||:
Mouse   500 SSKSFETKEQGSPEKARSSSSPQHLNVQQLQDMYSKMAKTQSQPILNTKEMAPQSQPQEELEMGS 564

  Fly   545 IPTPLAATPP--------------------------------PPVSS----AGGMTASGIGTTSA 573
            :|:|:|..|.                                .|::|    :||.||..:|...|
Mouse   565 MPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLASLSGKSGGSTAPEVGWRGA 629

  Fly   574 TGATSAPQPAT-PLPSIAVS 592
            .||:......| |:|..:.|
Mouse   630 LGASGGRLMETNPIPEASRS 649

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Shaw	NP_001137782.1	BTB	27..125	CDD:197585	47/107 (44%)
		BTB_2	27..117	CDD:280393	44/99 (44%)
		Ion_trans	244..439	CDD:278921	98/203 (48%)
		Ion_trans_2	355..429	CDD:285168	42/73 (58%)
Kcnb1	NP_032446.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		BTB_POZ_KCNB2	15..141	CDD:349719	49/109 (45%)
		Self-association. /evidence=ECO:0000250\|UniProtKB:P15387	59..75		3/16 (19%)
		Ion_trans	188..424	CDD:366146	110/263 (42%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	377..382		4/4 (100%)
		Self-association. /evidence=ECO:0000250\|UniProtKB:P15387	448..481		4/32 (13%)
		Kv2channel	467..678	CDD:367541	37/183 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	475..569		18/93 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	608..627		7/18 (39%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	768..802
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	816..857
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X19
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810