Protein Alignment HTR2B and Htr2b

DIOPT Version :10

• Sequence 1: NP_000858.3
• Gene: HTR2B / 3357
• HGNCID: 5294
• Length: 481
• Species: Homo sapiens
• Sequence 2: NP_058946.2
• Gene: Htr2b / 29581
• RGDID: 61801
• Length: 479
• Species: Rattus norvegicus
• Alignment Length: 481
• Identity: 393/481 - (81%)
• Similarity: 426/481 - (88%)
• Gaps: 2/481 - (0%)

Human     1 MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQGNKLHWAALLILMVI 65
            ||.||::|| ||||.|||||.|..|:|.::.|||:.||..|||||..|.|||.:|||||||..||
  Rat     1 MASSYKMSE-QSTISEHILQKTCDHLILTDRSGLKAESAAEEMKQTAENQGNTVHWAALLIFAVI 64

Human    66 IPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPA 130
            |||||||.||||||||||:|||||||||||||||||||||||||||||||||||.|||||.||||
  Rat    65 IPTIGGNILVILAVSLEKRLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEATWPLPLALCPA 129

Human   131 WLFLDVLFSTASIMHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGI 195
            ||||||||||||||||||||:||||||||||||||.|||.|||:|||||||||||||||||||||
  Rat   130 WLFLDVLFSTASIMHLCAISLDRYIAIKKPIQANQCNSRTTAFVKITVVWLISIGIAIPVPIKGI 194

Human   196 ETDVDNPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKNKPPQRLT 260
            |.||.|.:||||.|||:|||.|||||||||||.||.|||||||||||||:||||||:|:||||||
  Rat   195 EADVVNAHNITCELTKDRFGSFMLFGSLAAFFAPLTIMIVTYFLTIHALRKKAYLVRNRPPQRLT 259

Human   261 WLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDETLMRRTSTIGKKSVQTISNEQRASKV 325
            ..|||||.||:::..|||||:||||||.|||.||||.|||||||.|:.|||..||||||||||||
  Rat   260 RWTVSTVLQREDSSFSSPEKMAMLDGSHKDKILPNSIDETLMRRMSSAGKKPAQTISNEQRASKV 324

Human   326 LGIVFFLFLLMWCPFFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDA 390
            |||||..||||||||||||:||.||||||||||:.||:||||:||||||||||:|||||||||:|
  Rat   325 LGIVFLFFLLMWCPFFITNVTLALCDSCNQTTLKTLLQIFVWVGYVSSGVNPLIYTLFNKTFREA 389

Human   391 FGRYITCNYRATKSVKTLRKRSSKIYFRNPMAENSKFFKKHGIRNGINPAMYQSPMRLRSSTIQS 455
            |||||||||:||||||.|||.||.:||.|.|.||||||.||||||||||||||||:|||||||||
  Rat   390 FGRYITCNYQATKSVKVLRKCSSTLYFGNSMVENSKFFTKHGIRNGINPAMYQSPVRLRSSTIQS 454

Human   456 SSIILLDTLLLTENEGDKTEEQVSYV 481
            ||||||:| .||||:|||.|:||||:
  Rat   455 SSIILLNT-FLTENDGDKVEDQVSYI 479

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HTR2B	NP_000858.3	7tmA_5-HT2B	55..398	CDD:341347	293/342 (86%)
		TM helix 1	56..82	CDD:341347	22/25 (88%)
		TM helix 2	89..115	CDD:341347	25/25 (100%)
		TM helix 3	128..158	CDD:341347	28/29 (97%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000305|PubMed:23519215, ECO:0000305|PubMed:28129538	152..154		1/1 (100%)
		TM helix 4	170..193	CDD:341347	20/22 (91%)
		[DE]RFG motif, may stabilize a conformation that preferentially activates signaling via beta-arrestin family members. /evidence=ECO:0000305|PubMed:23519215, ECO:0000305|PubMed:28129538	212..215		1/2 (50%)
		TM helix 5	214..243	CDD:341347	25/28 (89%)
		TM helix 6	317..347	CDD:341347	26/29 (90%)
		TM helix 7	359..384	CDD:341347	19/24 (79%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000305|PubMed:23519215, ECO:0000305|PubMed:28129538	376..380		3/3 (100%)
		PDZ-binding. /evidence=ECO:0000269|PubMed:11150294	479..481		1/1 (100%)
Htr2b	NP_058946.2	7tm_GPCRs	54..397	CDD:475119	293/342 (86%)
		TM helix 1	56..80	CDD:410628	20/23 (87%)
		TM helix 2	89..111	CDD:410628	21/21 (100%)
		TM helix 3	128..150	CDD:410628	21/21 (100%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	151..153		1/1 (100%)
		TM helix 4	173..189	CDD:410628	14/15 (93%)
		[DE]RFG motif, may stabilize a conformation that preferentially activates signaling via beta-arrestin family members. /evidence=ECO:0000250|UniProtKB:P41595	211..214		1/2 (50%)
		TM helix 5	214..237	CDD:410628	19/22 (86%)
		TM helix 6	322..344	CDD:410628	19/21 (90%)
		TM helix 7	358..383	CDD:410628	19/24 (79%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	375..379		3/3 (100%)
		PDZ-binding. /evidence=ECO:0000250|UniProtKB:P41595	477..479		1/1 (100%)